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The map label for this gene is ddl

Identifier: 170757719

GI number: 170757719

Start: 570905

End: 571807

Strand: Direct

Name: ddl

Synonym: CLD_0290

Alternate gene names: 170757719

Gene position: 570905-571807 (Clockwise)

Preceding gene: 170756281

Following gene: 170754828

Centisome position: 14.42

GC content: 31.01

Gene sequence:

>903_bases
ATGAAAATTGGAGTTATAATGGGTGGAATATCTACAGAAAGAGAGGTTTCCTTGAATTCTGGTAGAGAAGTTATAAAATA
TTTAGAGCTTTTAGAGCATGAAATTATTCCTATTATTATAGATAAAAAAGAAGATGTTATGGAAAAAGCTAAGGGGATAG
ATTTTGCTTTCTTAGCATTGCATGGTAAATTTGGTGAAGATGGAACAGTTCAATCAGTTCTTCAAACTTTAGATATACCT
TATTCAGGATGTGGTCCTTTAACAAGCGCTATATGCATGGATAAGGATATGACTAAGAAAATTTTAAAATATGCTAATAT
AAATACAGCAGATTGGGTAAATGTATCTAGTGCAGAGAATATAGATTACGAAGCTATAGAAAAAATAGGGTACCCAGTGT
TCGTAAAACCTAATAGTGGTGGTTCTAGTGTAGCTACAAACTTAGTAAAAGATAAAGAGGGTATAAAAGAAGCAGTAGAA
CTTGCATTAAAATATGATAAAGAAGTTATGATAGAAAATTATACAAAAGGTGAAGAAATAACTTGCTGTATGCTAAATGG
CAAAATGTTACCAGTATTAGCTATAAGACCACATGCAGAATTCTTTGACTACACAGCAAAATATGCAGATGGTGGATCAG
ATGAAGTGGTTATAGAATTAGAAGAAAATCTTCATAAAAAGGTAGAAGAAATGGCCTTAGCTTGTTGGAAAGAATTGAAA
TGTGAAGTATATGTAAGGGTAGATATGATAGTTAAGGATGGAATTCCTTATGTATTAGAATTAAATACATTACCTGGTAT
GACCAAAAATAGCTTGTTCCCTAAGAGTGCTAATGCAGTAGGAATATCCTTTGCAGAATTATTAAATTCAATAGTTAAAT
ATTCATTAGAAGTAGAAAGATAA

Upstream 100 bases:

>100_bases
AGCAGGTTTAAATATAAATAGAAAGATGGTAAACATAAAGAACATTAAATTTAAAATTAATAGCAATGATATTAATAAAA
AGATATTGGAGGGGTAAATA

Downstream 100 bases:

>100_bases
TAAAATAAATTAGAATAAAAAATACTTATAGATTGCAAGTGATCTATAAGTATTTTTTATTTGAATGAAGTTTTTTTCCA
TGATATTCTGTTAAAGAATA

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MKIGVIMGGISTEREVSLNSGREVIKYLELLEHEIIPIIIDKKEDVMEKAKGIDFAFLALHGKFGEDGTVQSVLQTLDIP
YSGCGPLTSAICMDKDMTKKILKYANINTADWVNVSSAENIDYEAIEKIGYPVFVKPNSGGSSVATNLVKDKEGIKEAVE
LALKYDKEVMIENYTKGEEITCCMLNGKMLPVLAIRPHAEFFDYTAKYADGGSDEVVIELEENLHKKVEEMALACWKELK
CEVYVRVDMIVKDGIPYVLELNTLPGMTKNSLFPKSANAVGISFAELLNSIVKYSLEVER

Sequences:

>Translated_300_residues
MKIGVIMGGISTEREVSLNSGREVIKYLELLEHEIIPIIIDKKEDVMEKAKGIDFAFLALHGKFGEDGTVQSVLQTLDIP
YSGCGPLTSAICMDKDMTKKILKYANINTADWVNVSSAENIDYEAIEKIGYPVFVKPNSGGSSVATNLVKDKEGIKEAVE
LALKYDKEVMIENYTKGEEITCCMLNGKMLPVLAIRPHAEFFDYTAKYADGGSDEVVIELEENLHKKVEEMALACWKELK
CEVYVRVDMIVKDGIPYVLELNTLPGMTKNSLFPKSANAVGISFAELLNSIVKYSLEVER
>Mature_300_residues
MKIGVIMGGISTEREVSLNSGREVIKYLELLEHEIIPIIIDKKEDVMEKAKGIDFAFLALHGKFGEDGTVQSVLQTLDIP
YSGCGPLTSAICMDKDMTKKILKYANINTADWVNVSSAENIDYEAIEKIGYPVFVKPNSGGSSVATNLVKDKEGIKEAVE
LALKYDKEVMIENYTKGEEITCCMLNGKMLPVLAIRPHAEFFDYTAKYADGGSDEVVIELEENLHKKVEEMALACWKELK
CEVYVRVDMIVKDGIPYVLELNTLPGMTKNSLFPKSANAVGISFAELLNSIVKYSLEVER

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=307, Percent_Identity=39.0879478827362, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1786579, Length=349, Percent_Identity=29.2263610315186, Blast_Score=164, Evalue=7e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 33321; Mature: 33321

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGVIMGGISTEREVSLNSGREVIKYLELLEHEIIPIIIDKKEDVMEKAKGIDFAFLAL
CEEEEEECCCCCCCEECCCCCHHHHHHHHHHHHCCEEEEECCCHHHHHHHCCCCEEEEEE
HGKFGEDGTVQSVLQTLDIPYSGCGPLTSAICMDKDMTKKILKYANINTADWVNVSSAEN
ECCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHCCCCCCCEEECCCCCC
IDYEAIEKIGYPVFVKPNSGGSSVATNLVKDKEGIKEAVELALKYDKEVMIENYTKGEEI
CCHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCCE
TCCMLNGKMLPVLAIRPHAEFFDYTAKYADGGSDEVVIELEENLHKKVEEMALACWKELK
EEEEECCCEEEEEEECCCHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCC
CEVYVRVDMIVKDGIPYVLELNTLPGMTKNSLFPKSANAVGISFAELLNSIVKYSLEVER
EEEEEEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEECCC
>Mature Secondary Structure
MKIGVIMGGISTEREVSLNSGREVIKYLELLEHEIIPIIIDKKEDVMEKAKGIDFAFLAL
CEEEEEECCCCCCCEECCCCCHHHHHHHHHHHHCCEEEEECCCHHHHHHHCCCCEEEEEE
HGKFGEDGTVQSVLQTLDIPYSGCGPLTSAICMDKDMTKKILKYANINTADWVNVSSAEN
ECCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHCCCCCCCEEECCCCCC
IDYEAIEKIGYPVFVKPNSGGSSVATNLVKDKEGIKEAVELALKYDKEVMIENYTKGEEI
CCHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCCE
TCCMLNGKMLPVLAIRPHAEFFDYTAKYADGGSDEVVIELEENLHKKVEEMALACWKELK
EEEEECCCEEEEEEECCCHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCC
CEVYVRVDMIVKDGIPYVLELNTLPGMTKNSLFPKSANAVGISFAELLNSIVKYSLEVER
EEEEEEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA