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The map label for this gene is trmD

Identifier: 170757200

GI number: 170757200

Start: 2566591

End: 2567313

Strand: Reverse

Name: trmD

Synonym: CLD_2195

Alternate gene names: 170757200

Gene position: 2567313-2566591 (Counterclockwise)

Preceding gene: 170756645

Following gene: 170754562

Centisome position: 64.86

GC content: 30.01

Gene sequence:

>723_bases
ATGCGAATAGATGTGCTAACATTATTTCCACAAATGTTTTCTATATTTAATCACAGCATAATAGGAAGAGCTATAGAAAA
GGAAATTTTAAAAATAAACACTGTAAATATAAGGGATTATACAATAGATAAACATAAAAAAGTAGATGATTATCCTTATG
GAGGGGGAGCTGGCATGGTAATGGCAGCACAACCTATAGTAGATTCTATAAAGACAGTAAAAAAAGAAAACAAAGGGAAG
GTCATATTTTTAGGTCCTAAAGGGAAAACTTTTAATCAAAATTTGGCTAAAGAATTAGCGAAAGAGGAAGAATTAATATT
TTTATGTGGCCATTATGAAGGTATAGATGAAAGAGCCTATGAGTACATAGATATGGAAATATCCTTAGGTGATTTTGTTT
TAACGGGAGGAGAAATGGCTTGTATCCCTATAGTAGATTCCATATGTAGATTAGTAGATGGGGTGCTTAAAAGTAGCGAA
AGTTATGAAGATGAATCTTTTTATAATGGTCTTTTAGAATATCCTCAATATACAAGACCAGCTACATATGAAGGAAAATC
TGTGCCGGAGGTGCTTTTATCAGGTCACCATGAAAATATAAAAAAATGGCGAAAAGCTAAATCGCTCATAATTACTAATA
AAGTAAGACCAGATCTATTTAAAAAATATAAATTAACAGAAGAAGATAAGAAAATATTAAAAGATTTTAATAAAAAATTA
TAG

Upstream 100 bases:

>100_bases
AAGGAAAGAGCGAGGTATTAGTACCAGCCATAAAAGATATAGTTACAAATATAGATATAGAAAACAAAACTGTAACTATA
AAACCTTTGGAGATATGGCA

Downstream 100 bases:

>100_bases
GAAAAATATTTTTTAATTGTAAAAAATATTGAATAAAAATTTTTACTATGGTAAAATGAATTTTGTGCTAGTACGGGCGG
TCCTCTGCTTATATCATTTT

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MRIDVLTLFPQMFSIFNHSIIGRAIEKEILKINTVNIRDYTIDKHKKVDDYPYGGGAGMVMAAQPIVDSIKTVKKENKGK
VIFLGPKGKTFNQNLAKELAKEEELIFLCGHYEGIDERAYEYIDMEISLGDFVLTGGEMACIPIVDSICRLVDGVLKSSE
SYEDESFYNGLLEYPQYTRPATYEGKSVPEVLLSGHHENIKKWRKAKSLIITNKVRPDLFKKYKLTEEDKKILKDFNKKL

Sequences:

>Translated_240_residues
MRIDVLTLFPQMFSIFNHSIIGRAIEKEILKINTVNIRDYTIDKHKKVDDYPYGGGAGMVMAAQPIVDSIKTVKKENKGK
VIFLGPKGKTFNQNLAKELAKEEELIFLCGHYEGIDERAYEYIDMEISLGDFVLTGGEMACIPIVDSICRLVDGVLKSSE
SYEDESFYNGLLEYPQYTRPATYEGKSVPEVLLSGHHENIKKWRKAKSLIITNKVRPDLFKKYKLTEEDKKILKDFNKKL
>Mature_240_residues
MRIDVLTLFPQMFSIFNHSIIGRAIEKEILKINTVNIRDYTIDKHKKVDDYPYGGGAGMVMAAQPIVDSIKTVKKENKGK
VIFLGPKGKTFNQNLAKELAKEEELIFLCGHYEGIDERAYEYIDMEISLGDFVLTGGEMACIPIVDSICRLVDGVLKSSE
SYEDESFYNGLLEYPQYTRPATYEGKSVPEVLLSGHHENIKKWRKAKSLIITNKVRPDLFKKYKLTEEDKKILKDFNKKL

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=240, Percent_Identity=44.5833333333333, Blast_Score=215, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 27452; Mature: 27452

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIDVLTLFPQMFSIFNHSIIGRAIEKEILKINTVNIRDYTIDKHKKVDDYPYGGGAGMV
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCCCCCCCCCCCCCE
MAAQPIVDSIKTVKKENKGKVIFLGPKGKTFNQNLAKELAKEEELIFLCGHYEGIDERAY
EEHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHH
EYIDMEISLGDFVLTGGEMACIPIVDSICRLVDGVLKSSESYEDESFYNGLLEYPQYTRP
HHEEEEEECCCEEEECCCEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCC
ATYEGKSVPEVLLSGHHENIKKWRKAKSLIITNKVRPDLFKKYKLTEEDKKILKDFNKKL
CCCCCCCHHHHHHCCCHHHHHHHHHHHHHEEECCCCHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRIDVLTLFPQMFSIFNHSIIGRAIEKEILKINTVNIRDYTIDKHKKVDDYPYGGGAGMV
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCCCCCCCCCCCCCE
MAAQPIVDSIKTVKKENKGKVIFLGPKGKTFNQNLAKELAKEEELIFLCGHYEGIDERAY
EEHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHH
EYIDMEISLGDFVLTGGEMACIPIVDSICRLVDGVLKSSESYEDESFYNGLLEYPQYTRP
HHEEEEEECCCEEEECCCEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCC
ATYEGKSVPEVLLSGHHENIKKWRKAKSLIITNKVRPDLFKKYKLTEEDKKILKDFNKKL
CCCCCCCHHHHHHCCCHHHHHHHHHHHHHEEECCCCHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA