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The map label for this gene is yeiE [H]

Identifier: 170757070

GI number: 170757070

Start: 975157

End: 976044

Strand: Direct

Name: yeiE [H]

Synonym: CLD_3704

Alternate gene names: 170757070

Gene position: 975157-976044 (Clockwise)

Preceding gene: 170757848

Following gene: 170754594

Centisome position: 24.64

GC content: 29.05

Gene sequence:

>888_bases
ATGTATATTAATCTTGAACTTTACAGAATTTTTTATATTACTGCCAAACTAGGCAGTATATCTAAAGCAGCAAAGGAGCT
TTTTACATCACAGCCAGCAGTTAGTCAATCTATAAAATTATTAGAAGGAAAATTAGGTGGGGAATTATTTCATAGAACTC
CGAGAGGAGTTTCACTTACCTTAGAAGGTGAGGTACTATTCAAATATATAGAGCAAGGTTATGGTCTTATGATGACAGCG
GAAAGAAAATTTTCAGAATTAAAAAATCTTAATTCAGGTCAGATAAGAATAGCTGTATGTAGTGCAGTATGCAAATACTA
TCTAATGAAATATATAGAAACTTATAATCTAAATTATCCAAATATAAAAATTTATGTTAAGGATAAGTCAAGCCATGAAA
TAGCAAAAGCATTAGATTTAGGTGAGATAGATATAGGAATTTTAAATATGAATATAGACACAAGTAATTCTCTTAATGTT
ATTAGAACATTTAAAATACAAGATTGCTTTGTGGTAGGAGATAAGTACAAGGAAATTTGTGAAAAAGAGATAACATTGAA
GAATTTAGTGAATAGATATCCTATTATTTTACTAGAGAAAGGTGGTAATACAAGGAGTTATATAGATAATTATTTTAGTT
CACATGGATCGGAGGTAGTACCGCAAATAGAGTTAAGTAATGTAGAACTAATGATAGAATTTGCAAAAAGAGGCTTGGGA
GTTTCCTGTGTTATAAAAGATTATATAAAGAAGGAATTAGAACAGAAACAATTATATGAAATATCTATTAAAGAAAAAAT
CCCAGAGAGAATTATGGGCATAGCAACTAAAAAAGATATTCCTATGTCTACAGCAGCTGAGAGATTTATTGAACTTATTG
AAGATTAG

Upstream 100 bases:

>100_bases
ATGCTTTTAATTGTTGAATAACTTTTATAAAGCAATTATAATTAATATACCGATATAAGTAAAATTAATATCTAGTATAG
GAGGTATACTAAATTTTTAT

Downstream 100 bases:

>100_bases
ATTATTTATTTGAACTTTATCTTTAGGAGATGATAAGCTAGTTTCTTATATTGATGTAATTAAGATTAAAATTTAGCATT
GCTATTAAATTGTTTAAGGT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MYINLELYRIFYITAKLGSISKAAKELFTSQPAVSQSIKLLEGKLGGELFHRTPRGVSLTLEGEVLFKYIEQGYGLMMTA
ERKFSELKNLNSGQIRIAVCSAVCKYYLMKYIETYNLNYPNIKIYVKDKSSHEIAKALDLGEIDIGILNMNIDTSNSLNV
IRTFKIQDCFVVGDKYKEICEKEITLKNLVNRYPIILLEKGGNTRSYIDNYFSSHGSEVVPQIELSNVELMIEFAKRGLG
VSCVIKDYIKKELEQKQLYEISIKEKIPERIMGIATKKDIPMSTAAERFIELIED

Sequences:

>Translated_295_residues
MYINLELYRIFYITAKLGSISKAAKELFTSQPAVSQSIKLLEGKLGGELFHRTPRGVSLTLEGEVLFKYIEQGYGLMMTA
ERKFSELKNLNSGQIRIAVCSAVCKYYLMKYIETYNLNYPNIKIYVKDKSSHEIAKALDLGEIDIGILNMNIDTSNSLNV
IRTFKIQDCFVVGDKYKEICEKEITLKNLVNRYPIILLEKGGNTRSYIDNYFSSHGSEVVPQIELSNVELMIEFAKRGLG
VSCVIKDYIKKELEQKQLYEISIKEKIPERIMGIATKKDIPMSTAAERFIELIED
>Mature_295_residues
MYINLELYRIFYITAKLGSISKAAKELFTSQPAVSQSIKLLEGKLGGELFHRTPRGVSLTLEGEVLFKYIEQGYGLMMTA
ERKFSELKNLNSGQIRIAVCSAVCKYYLMKYIETYNLNYPNIKIYVKDKSSHEIAKALDLGEIDIGILNMNIDTSNSLNV
IRTFKIQDCFVVGDKYKEICEKEITLKNLVNRYPIILLEKGGNTRSYIDNYFSSHGSEVVPQIELSNVELMIEFAKRGLG
VSCVIKDYIKKELEQKQLYEISIKEKIPERIMGIATKKDIPMSTAAERFIELIED

Specific function: Positive Regulatory Protein For The Cyn Operon. [C]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=287, Percent_Identity=27.5261324041812, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1788481, Length=295, Percent_Identity=27.7966101694915, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI145693105, Length=290, Percent_Identity=25.8620689655172, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1788296, Length=238, Percent_Identity=27.3109243697479, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1786401, Length=300, Percent_Identity=26.3333333333333, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87081904, Length=294, Percent_Identity=24.8299319727891, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787530, Length=229, Percent_Identity=23.1441048034935, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788748, Length=302, Percent_Identity=21.8543046357616, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1789173, Length=139, Percent_Identity=30.2158273381295, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787879, Length=298, Percent_Identity=24.496644295302, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787601, Length=145, Percent_Identity=27.5862068965517, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1789204, Length=245, Percent_Identity=22.4489795918367, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787128, Length=259, Percent_Identity=23.1660231660232, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790399, Length=272, Percent_Identity=20.2205882352941, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788297, Length=250, Percent_Identity=22.8, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33694; Mature: 33694

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYINLELYRIFYITAKLGSISKAAKELFTSQPAVSQSIKLLEGKLGGELFHRTPRGVSLT
CEEEEEEEEEEEEEHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHCCCCCEEEE
LEGEVLFKYIEQGYGLMMTAERKFSELKNLNSGQIRIAVCSAVCKYYLMKYIETYNLNYP
ECHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCC
NIKIYVKDKSSHEIAKALDLGEIDIGILNMNIDTSNSLNVIRTFKIQDCFVVGDKYKEIC
EEEEEEECCCCHHHHHHHCCCCCEEEEEEEECCCCCCCCEEEEEEEEEEEEECHHHHHHH
EKEITLKNLVNRYPIILLEKGGNTRSYIDNYFSSHGSEVVPQIELSNVELMIEFAKRGLG
HHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHCCCC
VSCVIKDYIKKELEQKQLYEISIKEKIPERIMGIATKKDIPMSTAAERFIELIED
EEHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MYINLELYRIFYITAKLGSISKAAKELFTSQPAVSQSIKLLEGKLGGELFHRTPRGVSLT
CEEEEEEEEEEEEEHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHCCCCCEEEE
LEGEVLFKYIEQGYGLMMTAERKFSELKNLNSGQIRIAVCSAVCKYYLMKYIETYNLNYP
ECHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCC
NIKIYVKDKSSHEIAKALDLGEIDIGILNMNIDTSNSLNVIRTFKIQDCFVVGDKYKEIC
EEEEEEECCCCHHHHHHHCCCCCEEEEEEEECCCCCCCCEEEEEEEEEEEEECHHHHHHH
EKEITLKNLVNRYPIILLEKGGNTRSYIDNYFSSHGSEVVPQIELSNVELMIEFAKRGLG
HHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHCCCC
VSCVIKDYIKKELEQKQLYEISIKEKIPERIMGIATKKDIPMSTAAERFIELIED
EEHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]