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The map label for this gene is chiA [H]

Identifier: 170757004

GI number: 170757004

Start: 1206404

End: 1208026

Strand: Direct

Name: chiA [H]

Synonym: CLD_3478

Alternate gene names: 170757004

Gene position: 1206404-1208026 (Clockwise)

Preceding gene: 170754272

Following gene: 170757756

Centisome position: 30.48

GC content: 35.86

Gene sequence:

>1623_bases
ATGAAATCCAAAAAACTTACTGCACTTGTTTTATCAGTATTAGTAGCTTCAGGAGCTACTTCGGCAATTCTTACAGAAAA
AAATATAGTACAAGCTAAAGAGGTAAAAATCAGTAGTGTACAGTCTAAAATAGCTACTAATATAGGAATAGGAGAAGAAT
CTTGGGGATCTACTGTCTTCGCACCATATGTAGATGCTTGTTCATACCCTGTGTTCTCCCTTACTGAATACGCAAAGGAA
ACAGGAACTAAACATTTTATATTGGGATTTGTAGTTGATAAAAATGGACAACCTTCCTGGGGAACTTATTATGATATGGA
AGAAGGGCCTACAGATTATCAAGGTGGTGGATTATTAAAAGAAATTAAAGCTTTAAGACAAATGGGCGGAGATGTAATGG
TATCTTTTGGGGGTGAAGCAAATACACCATTAGCAGCATCAATTAAAGATGTTAATAAATTAAAAGATGCATATAAAAAA
GTAATTAAAGACTATGGATTAACTCATATAGATTTTGATATTGAAGGAGCTTGGACAGCTGATGCGGCTTCTATAGAAAG
ACGTTCACAAGCTATTGCATTACTTCAAAAGGAACTTAAAGCTGAAAATCATCCACTTAAAATATGGTATACATTACCCG
TATTGCCTTCAGGGATGACTAATGGGGTAGATGTGGTAAAGAGTGCTGTTAAAAATGGTGTTGAGCTAAGTGGAGTAAAT
GTAATGACTATGTGTTTTGGACAGAAGGCTCCAGATGGAACTATGGGAGATTTAGTTATTGAGTCAGCTAAAAGTGTTCA
TAAACAATTAAAGGATATATACACACAAGCTAATATACAAAAGTCTGATAAAGAAATTTGGAAAATGATAGGAGTAACTC
CTATGAATGGTTTAGATTATAGTGGAAGTATACTTGATCAAAACGGAGCAAGAAAGGTTGCGAGCTTTGGTAAAACAGTA
GGAATGGGACTTATAGGTATGTGGTCAGTAAATAGAGATTATCAAAATCCTAAGGGTTCAACAAGTTACGTATCTATAAC
GGATAGTTCTATATTACAAGAAAAATTTGAGTTTGGTAAAATACTTTCAAATTATGATTCAAATGATGGTTCAGGTATAA
TAGAGAAACCAGGTGATAGTGAAGATGGTGAAGATGGTCAAGATGGTCAAGATGGTCAAGATGGAGGAGAAGTAGAAGGT
GTTCCTGCATATAAGCCACAACAAGAATATTCGGCAGGAGATAAAGTTAGCTATAAAGGGAAGATTTATCAAGCCAAATG
GTGGACAAAAGGGTTTGCACCAGATACGGCAGTACAAAATTCCTGGGATACACCGTGGGAACTAATAGGGAATTCAGATG
ATGGTAAAGATGTTGAAGACGGTAAAGATGTTGAAGATGGTAAAGATGTTGAAGACGGTAAAGATGTTGAAGATGGTAAA
GATATTGAAGATGGAGGAGAAGTAGGAAGCGCTCCTGCATATAATCTACAACAAGAATATTGGGGAGGAGATAAAGTTAG
TTACAAAGGAAAGGTTTATCAAGCCAAATGGTGGACAAAAGGATTTGCGCCAGATACAGTAGTAGAAAATACATGGGATA
CACCTTGGGAGCTAATAGGATAA

Upstream 100 bases:

>100_bases
ATTTCTCAAATGATAAATAATACCCCAAATAGTAGTTAAAAAATTACTAAGGTTTTACTATTTATCTTATTAAAATATTA
ATGATGTGGAGGTATATATT

Downstream 100 bases:

>100_bases
GATATAGTATGATAAAACTAAATATATAAATTTTTTAAGAGTATTGGGTTAGAGGTATATTTCTCTCTAATCCAATACTT
TATTTTAATCAATAAACATA

Product: putative glycosyl hydrolase

Products: NA

Alternate protein names: Chitinase; Lysozyme [H]

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MKSKKLTALVLSVLVASGATSAILTEKNIVQAKEVKISSVQSKIATNIGIGEESWGSTVFAPYVDACSYPVFSLTEYAKE
TGTKHFILGFVVDKNGQPSWGTYYDMEEGPTDYQGGGLLKEIKALRQMGGDVMVSFGGEANTPLAASIKDVNKLKDAYKK
VIKDYGLTHIDFDIEGAWTADAASIERRSQAIALLQKELKAENHPLKIWYTLPVLPSGMTNGVDVVKSAVKNGVELSGVN
VMTMCFGQKAPDGTMGDLVIESAKSVHKQLKDIYTQANIQKSDKEIWKMIGVTPMNGLDYSGSILDQNGARKVASFGKTV
GMGLIGMWSVNRDYQNPKGSTSYVSITDSSILQEKFEFGKILSNYDSNDGSGIIEKPGDSEDGEDGQDGQDGQDGGEVEG
VPAYKPQQEYSAGDKVSYKGKIYQAKWWTKGFAPDTAVQNSWDTPWELIGNSDDGKDVEDGKDVEDGKDVEDGKDVEDGK
DIEDGGEVGSAPAYNLQQEYWGGDKVSYKGKVYQAKWWTKGFAPDTVVENTWDTPWELIG

Sequences:

>Translated_540_residues
MKSKKLTALVLSVLVASGATSAILTEKNIVQAKEVKISSVQSKIATNIGIGEESWGSTVFAPYVDACSYPVFSLTEYAKE
TGTKHFILGFVVDKNGQPSWGTYYDMEEGPTDYQGGGLLKEIKALRQMGGDVMVSFGGEANTPLAASIKDVNKLKDAYKK
VIKDYGLTHIDFDIEGAWTADAASIERRSQAIALLQKELKAENHPLKIWYTLPVLPSGMTNGVDVVKSAVKNGVELSGVN
VMTMCFGQKAPDGTMGDLVIESAKSVHKQLKDIYTQANIQKSDKEIWKMIGVTPMNGLDYSGSILDQNGARKVASFGKTV
GMGLIGMWSVNRDYQNPKGSTSYVSITDSSILQEKFEFGKILSNYDSNDGSGIIEKPGDSEDGEDGQDGQDGQDGGEVEG
VPAYKPQQEYSAGDKVSYKGKIYQAKWWTKGFAPDTAVQNSWDTPWELIGNSDDGKDVEDGKDVEDGKDVEDGKDVEDGK
DIEDGGEVGSAPAYNLQQEYWGGDKVSYKGKVYQAKWWTKGFAPDTVVENTWDTPWELIG
>Mature_540_residues
MKSKKLTALVLSVLVASGATSAILTEKNIVQAKEVKISSVQSKIATNIGIGEESWGSTVFAPYVDACSYPVFSLTEYAKE
TGTKHFILGFVVDKNGQPSWGTYYDMEEGPTDYQGGGLLKEIKALRQMGGDVMVSFGGEANTPLAASIKDVNKLKDAYKK
VIKDYGLTHIDFDIEGAWTADAASIERRSQAIALLQKELKAENHPLKIWYTLPVLPSGMTNGVDVVKSAVKNGVELSGVN
VMTMCFGQKAPDGTMGDLVIESAKSVHKQLKDIYTQANIQKSDKEIWKMIGVTPMNGLDYSGSILDQNGARKVASFGKTV
GMGLIGMWSVNRDYQNPKGSTSYVSITDSSILQEKFEFGKILSNYDSNDGSGIIEKPGDSEDGEDGQDGQDGQDGGEVEG
VPAYKPQQEYSAGDKVSYKGKIYQAKWWTKGFAPDTAVQNSWDTPWELIGNSDDGKDVEDGKDVEDGKDVEDGKDVEDGK
DIEDGGEVGSAPAYNLQQEYWGGDKVSYKGKVYQAKWWTKGFAPDTVVENTWDTPWELIG

Specific function: Bifunctional enzyme with lysozyme/chitinase activity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 5 chitin-binding type-3 domains [H]

Homologues:

Organism=Escherichia coli, GI1789736, Length=298, Percent_Identity=40.2684563758389, Blast_Score=209, Evalue=3e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003610
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF02839 CBM_5_12 [H]

EC number: =3.2.1.14; =3.2.1.17 [H]

Molecular weight: Translated: 58722; Mature: 58722

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSKKLTALVLSVLVASGATSAILTEKNIVQAKEVKISSVQSKIATNIGIGEESWGSTVF
CCCHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEH
APYVDACSYPVFSLTEYAKETGTKHFILGFVVDKNGQPSWGTYYDMEEGPTDYQGGGLLK
HHHHHHHCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCEEECCCCCCCCCCCCCHHH
EIKALRQMGGDVMVSFGGEANTPLAASIKDVNKLKDAYKKVIKDYGLTHIDFDIEGAWTA
HHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCC
DAASIERRSQAIALLQKELKAENHPLKIWYTLPVLPSGMTNGVDVVKSAVKNGVELSGVN
CHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCEECCCE
VMTMCFGQKAPDGTMGDLVIESAKSVHKQLKDIYTQANIQKSDKEIWKMIGVTPMNGLDY
EEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCC
SGSILDQNGARKVASFGKTVGMGLIGMWSVNRDYQNPKGSTSYVSITDSSILQEKFEFGK
CCCEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEECCHHHHHHHHHHHH
ILSNYDSNDGSGIIEKPGDSEDGEDGQDGQDGQDGGEVEGVPAYKPQQEYSAGDKVSYKG
HHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCEEECC
KIYQAKWWTKGFAPDTAVQNSWDTPWELIGNSDDGKDVEDGKDVEDGKDVEDGKDVEDGK
EEEEEECCCCCCCCCCHHCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DIEDGGEVGSAPAYNLQQEYWGGDKVSYKGKVYQAKWWTKGFAPDTVVENTWDTPWELIG
CCCCCCCCCCCCCCCCHHHHCCCCEEEECCEEEEEECCCCCCCCCCHHCCCCCCCHHHCC
>Mature Secondary Structure
MKSKKLTALVLSVLVASGATSAILTEKNIVQAKEVKISSVQSKIATNIGIGEESWGSTVF
CCCHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEH
APYVDACSYPVFSLTEYAKETGTKHFILGFVVDKNGQPSWGTYYDMEEGPTDYQGGGLLK
HHHHHHHCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCEEECCCCCCCCCCCCCHHH
EIKALRQMGGDVMVSFGGEANTPLAASIKDVNKLKDAYKKVIKDYGLTHIDFDIEGAWTA
HHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCC
DAASIERRSQAIALLQKELKAENHPLKIWYTLPVLPSGMTNGVDVVKSAVKNGVELSGVN
CHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCEECCCE
VMTMCFGQKAPDGTMGDLVIESAKSVHKQLKDIYTQANIQKSDKEIWKMIGVTPMNGLDY
EEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCC
SGSILDQNGARKVASFGKTVGMGLIGMWSVNRDYQNPKGSTSYVSITDSSILQEKFEFGK
CCCEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEECCHHHHHHHHHHHH
ILSNYDSNDGSGIIEKPGDSEDGEDGQDGQDGQDGGEVEGVPAYKPQQEYSAGDKVSYKG
HHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCEEECC
KIYQAKWWTKGFAPDTAVQNSWDTPWELIGNSDDGKDVEDGKDVEDGKDVEDGKDVEDGK
EEEEEECCCCCCCCCCHHCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DIEDGGEVGSAPAYNLQQEYWGGDKVSYKGKVYQAKWWTKGFAPDTVVENTWDTPWELIG
CCCCCCCCCCCCCCCCHHHHCCCCEEEECCEEEEEECCCCCCCCCCHHCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 2661540; 10419961; 10760150 [H]