Click here to switch to the map view.

The map label for this gene is greA

Identifier: 170756932

GI number: 170756932

Start: 3833272

End: 3833754

Strand: Reverse

Name: greA

Synonym: CLD_0971

Alternate gene names: 170756932

Gene position: 3833754-3833272 (Counterclockwise)

Preceding gene: 170755264

Following gene: 170757532

Centisome position: 96.86

GC content: 29.81

Gene sequence:

>483_bases
ATGAGCGAAGCAAAAAAATATGTTATGACTTATGAAGGGGTAAAGAAATTAGAAGAAGAATTAGAATATCTAAAAACGGT
AAAGAGAAAAGAAATAACAGAAAAAATAAAAGTAGCTCTTTCATTTGGTGATTTAAGCGAAAATTCAGAATATGATGAAG
CTAAAAATGAACAAGCTTTTGTAGAAGGAAGAATAATACAACTAGAAAACATGCTAAAAAATGCTAGTATAGTTGATGAA
AATGAAGTGCCTAAAGATATAGTAAGTGTGGGCTCTATAGTAAAGGTTAAAGATTATGAATTTGATGAAGAAGTTGAATA
TATAATTGTAGGCTCTGCTGAAGCTGACCCTATGAATAATAAAATATCTAATGAATCGCCAGTAGGACATGGATTAATAG
GTAAAAAAGTTGGAGATATAATAGAAGTAACGGTTCCAGATGGTGTTAGTAAATATGAAATATTAGAGGTTAACAGAGCA
TAG

Upstream 100 bases:

>100_bases
AAATTACCTGTTTTATAATTACTTAAATGTTTCTTTTATGTTATAATGTTAAGAATGTATCATAATATATAAATAGTAAC
AAAATAAAGGGGAGAATAGC

Downstream 100 bases:

>100_bases
TATTGGAGGGAGTTTATAGTGTCAAAGGAAGATAATGTGATAAACAGTTTTGAAGAACAAGCAAATGAATTAATGAAGGA
AAGATTCCAAAAGTTAAAGG

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MSEAKKYVMTYEGVKKLEEELEYLKTVKRKEITEKIKVALSFGDLSENSEYDEAKNEQAFVEGRIIQLENMLKNASIVDE
NEVPKDIVSVGSIVKVKDYEFDEEVEYIIVGSAEADPMNNKISNESPVGHGLIGKKVGDIIEVTVPDGVSKYEILEVNRA

Sequences:

>Translated_160_residues
MSEAKKYVMTYEGVKKLEEELEYLKTVKRKEITEKIKVALSFGDLSENSEYDEAKNEQAFVEGRIIQLENMLKNASIVDE
NEVPKDIVSVGSIVKVKDYEFDEEVEYIIVGSAEADPMNNKISNESPVGHGLIGKKVGDIIEVTVPDGVSKYEILEVNRA
>Mature_159_residues
SEAKKYVMTYEGVKKLEEELEYLKTVKRKEITEKIKVALSFGDLSENSEYDEAKNEQAFVEGRIIQLENMLKNASIVDEN
EVPKDIVSVGSIVKVKDYEFDEEVEYIIVGSAEADPMNNKISNESPVGHGLIGKKVGDIIEVTVPDGVSKYEILEVNRA

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=150, Percent_Identity=43.3333333333333, Blast_Score=117, Evalue=4e-28,
Organism=Escherichia coli, GI87082261, Length=149, Percent_Identity=34.2281879194631, Blast_Score=75, Evalue=2e-15,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 17963; Mature: 17831

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAKKYVMTYEGVKKLEEELEYLKTVKRKEITEKIKVALSFGDLSENSEYDEAKNEQAF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHCCCHHH
VEGRIIQLENMLKNASIVDENEVPKDIVSVGSIVKVKDYEFDEEVEYIIVGSAEADPMNN
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCEEEEECCCCCCCCCEEEEECCCCCCCCC
KISNESPVGHGLIGKKVGDIIEVTVPDGVSKYEILEVNRA
CCCCCCCCCCCCHHHCCCCEEEEECCCCCCCEEEEEECCC
>Mature Secondary Structure 
SEAKKYVMTYEGVKKLEEELEYLKTVKRKEITEKIKVALSFGDLSENSEYDEAKNEQAF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHCCCHHH
VEGRIIQLENMLKNASIVDENEVPKDIVSVGSIVKVKDYEFDEEVEYIIVGSAEADPMNN
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCEEEEECCCCCCCCCEEEEECCCCCCCCC
KISNESPVGHGLIGKKVGDIIEVTVPDGVSKYEILEVNRA
CCCCCCCCCCCCHHHCCCCEEEEECCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA