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The map label for this gene is trmB

Identifier: 170756894

GI number: 170756894

Start: 615435

End: 616088

Strand: Direct

Name: trmB

Synonym: CLD_0249

Alternate gene names: 170756894

Gene position: 615435-616088 (Clockwise)

Preceding gene: 170757600

Following gene: 170757236

Centisome position: 15.55

GC content: 24.77

Gene sequence:

>654_bases
ATGAGACTTAGAAAAAAATGGTGGGCAAGACCGGAGATAGAAGCTAGTGATAAATTTGCAGAGGAACCTAAAGAATTAAG
AGGAAAATGGAATAAGGAATTTAATAATAATAATGATATACATTTAGAATTAGGTTGTGGAAGAGGAGGATTTATATCAC
AATTAGTAGAAAAAAATAAAGATATAAACTATGTAGGAATAGATTTAAAGGATGAAGTTATAGTTTATGCTATAAGAAAA
GTTAAAGAAAAGGAAGAAGAAGTTAAAAGAGAGTTTAAAAATATAAGATTTGTTACAATGAATATAATGGGAATAGCAGA
AGTATTTGATAAAAATGAAATAAGTAAAATATATATAAATTTTTGCAACCCTTGGCCAAAAGAAAAGCATAATAAAAGAA
GATTAACCCATACAAAATTACTAACGGAATATAAAAAATTTTTAAAACCAAATACAGAAATATGGTTTAAAACTGATGAC
AAAGAACTTTTTGAGGATTCACAAGAATATTTTAAGGAAAGTGGATTTAATATAGAATACATCACATATGATCTTCATAA
TAGTGATTTTAAAGAGAATATAAAAACAGAATATGAAACAAAATTTGAAACTATGGGAATGAAAATAATGTTTTTAAAGG
CTAGATTATTATAA

Upstream 100 bases:

>100_bases
TTGTTTTTTAAAATATAATTAAATATAGATTACTAATAATAATAGGTGTTATGATATAATATTTCAGTAGTAGATTAAAA
AGATATCTGGAGGTTAAAAA

Downstream 100 bases:

>100_bases
AAGAATTTATATAGAATGATATTTATTATAAATTGTTTACTATAGCATAGATTTTGCGAAAGATGTTTTACAAGAGTAAA
GTGAGAGTTAATACATAAAG

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MRLRKKWWARPEIEASDKFAEEPKELRGKWNKEFNNNNDIHLELGCGRGGFISQLVEKNKDINYVGIDLKDEVIVYAIRK
VKEKEEEVKREFKNIRFVTMNIMGIAEVFDKNEISKIYINFCNPWPKEKHNKRRLTHTKLLTEYKKFLKPNTEIWFKTDD
KELFEDSQEYFKESGFNIEYITYDLHNSDFKENIKTEYETKFETMGMKIMFLKARLL

Sequences:

>Translated_217_residues
MRLRKKWWARPEIEASDKFAEEPKELRGKWNKEFNNNNDIHLELGCGRGGFISQLVEKNKDINYVGIDLKDEVIVYAIRK
VKEKEEEVKREFKNIRFVTMNIMGIAEVFDKNEISKIYINFCNPWPKEKHNKRRLTHTKLLTEYKKFLKPNTEIWFKTDD
KELFEDSQEYFKESGFNIEYITYDLHNSDFKENIKTEYETKFETMGMKIMFLKARLL
>Mature_217_residues
MRLRKKWWARPEIEASDKFAEEPKELRGKWNKEFNNNNDIHLELGCGRGGFISQLVEKNKDINYVGIDLKDEVIVYAIRK
VKEKEEEVKREFKNIRFVTMNIMGIAEVFDKNEISKIYINFCNPWPKEKHNKRRLTHTKLLTEYKKFLKPNTEIWFKTDD
KELFEDSQEYFKESGFNIEYITYDLHNSDFKENIKTEYETKFETMGMKIMFLKARLL

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 26259; Mature: 26259

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLRKKWWARPEIEASDKFAEEPKELRGKWNKEFNNNNDIHLELGCGRGGFISQLVEKNK
CCCCCHHCCCCCCCCCHHHHCCHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCC
DINYVGIDLKDEVIVYAIRKVKEKEEEVKREFKNIRFVTMNIMGIAEVFDKNEISKIYIN
CCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEHHHHHHHHCCCCCEEEEEE
FCNPWPKEKHNKRRLTHTKLLTEYKKFLKPNTEIWFKTDDKELFEDSQEYFKESGFNIEY
ECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEE
ITYDLHNSDFKENIKTEYETKFETMGMKIMFLKARLL
EEEECCCCCHHHHHHHHHHHHHHHHCEEEEEEEECCC
>Mature Secondary Structure
MRLRKKWWARPEIEASDKFAEEPKELRGKWNKEFNNNNDIHLELGCGRGGFISQLVEKNK
CCCCCHHCCCCCCCCCHHHHCCHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCC
DINYVGIDLKDEVIVYAIRKVKEKEEEVKREFKNIRFVTMNIMGIAEVFDKNEISKIYIN
CCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEHHHHHHHHCCCCCEEEEEE
FCNPWPKEKHNKRRLTHTKLLTEYKKFLKPNTEIWFKTDDKELFEDSQEYFKESGFNIEY
ECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEE
ITYDLHNSDFKENIKTEYETKFETMGMKIMFLKARLL
EEEECCCCCHHHHHHHHHHHHHHHHCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA