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The map label for this gene is hypB

Identifier: 170756885

GI number: 170756885

Start: 546780

End: 547337

Strand: Direct

Name: hypB

Synonym: CLD_0312

Alternate gene names: 170756885

Gene position: 546780-547337 (Clockwise)

Preceding gene: 170754283

Following gene: 170756902

Centisome position: 13.81

GC content: 33.33

Gene sequence:

>558_bases
ATGTCATCACCTGGTTCAGGAAAAACAACAACTCTTTTAAGAACAGTTAAGGCTTTGAAAAATGAAATGAATATTGGGGT
TCTGGAAGCAGATATTGATTCAGAGGTAGATGCAAATAAGGTTTCACAGTCTGGTGTTAAAGTAATTCAGCTACACACTG
GAGGAATGTGCCATCTTGATGCTGATATGACAAAACAAAGTTTAAATGGTCTTGGGACTGAGAATATTGATTTAGCAGTA
CTAGAGAATATTGGGAATTTAGTATGTCCCGCAGAGTTTGATACTGGTGCATCAAGAAATGCTATGATTTTAAGCATACC
AGAAGGGGATGATAAACCCTTAAAATATCCTTTAATGTTTTCAATAGTTGATGTTCTACTAATTAATAAAATAGATGCAA
AGGACTATTTTGAATTTGATTTGGAAGCAGTAAAAGAAAGGGTAAGAAAACTAAATCCAAATATTAAGGTTATTCCAATT
TCAGCAAAGACTGGAGAGGGAATTGAAGAATGGATTGATTGGATACGTAAAGAAGTAAAGATTTGGAAAGAAAATTAA

Upstream 100 bases:

>100_bases
AAGTTCTAGAAATCAAACAAAGTGTTTTCGAAAATAATGATCGCCAAGCAGAGTTACTTAGAGAAGAATTGAAAAAAAGG
GGATATTTTTATTAAATCTT

Downstream 100 bases:

>100_bases
GTGAAAATTCATAATGCTATTGATGACATAAAAAATTCTTATATAATTTTGATGATATATAATAATCAGCTTTTTCGGTT
ATTTTTGTTTTAAAAACTTA

Product: hydrogenase accessory protein HypB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 185; Mature: 184

Protein sequence:

>185_residues
MSSPGSGKTTTLLRTVKALKNEMNIGVLEADIDSEVDANKVSQSGVKVIQLHTGGMCHLDADMTKQSLNGLGTENIDLAV
LENIGNLVCPAEFDTGASRNAMILSIPEGDDKPLKYPLMFSIVDVLLINKIDAKDYFEFDLEAVKERVRKLNPNIKVIPI
SAKTGEGIEEWIDWIRKEVKIWKEN

Sequences:

>Translated_185_residues
MSSPGSGKTTTLLRTVKALKNEMNIGVLEADIDSEVDANKVSQSGVKVIQLHTGGMCHLDADMTKQSLNGLGTENIDLAV
LENIGNLVCPAEFDTGASRNAMILSIPEGDDKPLKYPLMFSIVDVLLINKIDAKDYFEFDLEAVKERVRKLNPNIKVIPI
SAKTGEGIEEWIDWIRKEVKIWKEN
>Mature_184_residues
SSPGSGKTTTLLRTVKALKNEMNIGVLEADIDSEVDANKVSQSGVKVIQLHTGGMCHLDADMTKQSLNGLGTENIDLAVL
ENIGNLVCPAEFDTGASRNAMILSIPEGDDKPLKYPLMFSIVDVLLINKIDAKDYFEFDLEAVKERVRKLNPNIKVIPIS
AKTGEGIEEWIDWIRKEVKIWKEN

Specific function: Could be involved in nickel binding and accumulation [H]

COG id: COG0378

COG function: function code OK; Ni2+-binding GTPase involved in regulation of expression and maturation of urease and hydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypB/hupM family [H]

Homologues:

Organism=Escherichia coli, GI1789082, Length=178, Percent_Identity=42.6966292134831, Blast_Score=164, Evalue=4e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003495
- InterPro:   IPR004392
- InterPro:   IPR012202 [H]

Pfam domain/function: PF02492 cobW [H]

EC number: NA

Molecular weight: Translated: 20441; Mature: 20310

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPGSGKTTTLLRTVKALKNEMNIGVLEADIDSEVDANKVSQSGVKVIQLHTGGMCHLD
CCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHCCCCEEEEEECCCEEEEC
ADMTKQSLNGLGTENIDLAVLENIGNLVCPAEFDTGASRNAMILSIPEGDDKPLKYPLMF
HHHHHHHHCCCCCCCCHHHHHHHCCCEECCCCCCCCCCCCEEEEECCCCCCCCCCCHHHH
SIVDVLLINKIDAKDYFEFDLEAVKERVRKLNPNIKVIPISAKTGEGIEEWIDWIRKEVK
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
IWKEN
HHCCC
>Mature Secondary Structure 
SSPGSGKTTTLLRTVKALKNEMNIGVLEADIDSEVDANKVSQSGVKVIQLHTGGMCHLD
CCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHCCCCEEEEEECCCEEEEC
ADMTKQSLNGLGTENIDLAVLENIGNLVCPAEFDTGASRNAMILSIPEGDDKPLKYPLMF
HHHHHHHHCCCCCCCCHHHHHHHCCCEECCCCCCCCCCCCEEEEECCCCCCCCCCCHHHH
SIVDVLLINKIDAKDYFEFDLEAVKERVRKLNPNIKVIPISAKTGEGIEEWIDWIRKEVK
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
IWKEN
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8486288 [H]