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The map label for this gene is nudG [H]

Identifier: 170756877

GI number: 170756877

Start: 779218

End: 779616

Strand: Reverse

Name: nudG [H]

Synonym: CLD_0106

Alternate gene names: 170756877

Gene position: 779616-779218 (Counterclockwise)

Preceding gene: 170755518

Following gene: 170755788

Centisome position: 19.7

GC content: 31.08

Gene sequence:

>399_bases
ATGAAAAAGTTAATTAAAGTTGTTGGTGCTATTATTGAAAATGAAAATAATGAAATCTTATGTGCCTTAAGATCAACTAA
AATGTCTCTTCCTAACCTATGGGAATTTCCTGGCGGTAAAATCGAAAAAGGTGAATCTTTAGCTGATGCTATAGTTCGCG
AAATTAAAGAAGAGCTTGACTGTACTATAAGCTTTATAGATGTTTTTAATGAAAACACTCATGAATATGATAAATTTATA
GTTAATTTGACAACTGCTAGATGTAAATTAATAGAAGGGAAACCTACTGCTAATGAACACGATAAGCTTATTTGGCTTTC
TAAAGAAAATTTAATTTCCTTAAATTGGGCTCCTGCTGATATTCCCGCTGTGGAGCAATTAGCTAAAGAAAAAATGTAG

Upstream 100 bases:

>100_bases
AAAAAATTATATCCAATAACTCTTATCCAAATATACATTAAGCTTGTATTATTGACTTAAACAATGTTATTTAGTTAAAA
TGTTATTGGAGGTGAAATAC

Downstream 100 bases:

>100_bases
CACCGCTAATAATAGTGCTACATCTTTTTCTTTATATGTCTATGATATTACATAATCATAAATATCTTCTCTTACTTTCG
TAATTAATTTATATCTTATT

Product: putative mutator mutT protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 132; Mature: 132

Protein sequence:

>132_residues
MKKLIKVVGAIIENENNEILCALRSTKMSLPNLWEFPGGKIEKGESLADAIVREIKEELDCTISFIDVFNENTHEYDKFI
VNLTTARCKLIEGKPTANEHDKLIWLSKENLISLNWAPADIPAVEQLAKEKM

Sequences:

>Translated_132_residues
MKKLIKVVGAIIENENNEILCALRSTKMSLPNLWEFPGGKIEKGESLADAIVREIKEELDCTISFIDVFNENTHEYDKFI
VNLTTARCKLIEGKPTANEHDKLIWLSKENLISLNWAPADIPAVEQLAKEKM
>Mature_132_residues
MKKLIKVVGAIIENENNEILCALRSTKMSLPNLWEFPGGKIEKGESLADAIVREIKEELDCTISFIDVFNENTHEYDKFI
VNLTTARCKLIEGKPTANEHDKLIWLSKENLISLNWAPADIPAVEQLAKEKM

Specific function: Specific for pyrimidine substrates. Acts on 5-methyl- dCTP, CTP and dCTP in decreasing order [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1788056, Length=123, Percent_Identity=38.2113821138211, Blast_Score=84, Evalue=2e-18,
Organism=Escherichia coli, GI1786288, Length=129, Percent_Identity=36.4341085271318, Blast_Score=78, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.-

Molecular weight: Translated: 14968; Mature: 14968

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLIKVVGAIIENENNEILCALRSTKMSLPNLWEFPGGKIEKGESLADAIVREIKEELD
CHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
CTISFIDVFNENTHEYDKFIVNLTTARCKLIEGKPTANEHDKLIWLSKENLISLNWAPAD
CCHHHHHHHCCCCHHHHHHHEEEEHHEEEEECCCCCCCCCCCEEEEECCCEEEECCCCCC
IPAVEQLAKEKM
CHHHHHHHHHCC
>Mature Secondary Structure
MKKLIKVVGAIIENENNEILCALRSTKMSLPNLWEFPGGKIEKGESLADAIVREIKEELD
CHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
CTISFIDVFNENTHEYDKFIVNLTTARCKLIEGKPTANEHDKLIWLSKENLISLNWAPAD
CCHHHHHHHCCCCHHHHHHHEEEEHHEEEEECCCCCCCCCCCEEEEECCCEEEECCCCCC
IPAVEQLAKEKM
CHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe; Mn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 11053429 [H]