| Definition | Clostridium botulinum B1 str. Okra, complete genome. |
|---|---|
| Accession | NC_010516 |
| Length | 3,958,233 |
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The map label for this gene is pps [H]
Identifier: 170756675
GI number: 170756675
Start: 2757263
End: 2759740
Strand: Reverse
Name: pps [H]
Synonym: CLD_1983
Alternate gene names: 170756675
Gene position: 2759740-2757263 (Counterclockwise)
Preceding gene: 170754953
Following gene: 170755612
Centisome position: 69.72
GC content: 28.45
Gene sequence:
>2478_bases ATGGACACTAAAAATATTATTGGTATAAAAGATGCACGGGAAAATCAAATTGATATTGTAGGAGGAAAAGGGGCTAATTT AGGACTAATGATTTCATATGGCATTCCTGTTCCAGATGGCTTTATTGTAACGGCTAATGCATATAAAAATTTTTTAAAAA GTAATGGTATATTAGAAATAATAGAACAAAAAATTAGTAATATAGATAAAGAAACTTTATCTATAAAGGGTAAAACTGAA GAGATAAGAAATTTAATTTTAAAAGCTGAGTTCCCAAAGGACTTAAAAGAAGATATATTGAGTAGATTTAATAAATTTAA GAGACCTATTCACTTAGCAGTAAGATCTTCGGCAACAGCCGAGGATTTACCGGAAGCAAGTTTTGCAGGGCAACAAGAGA CATATTTGAATATTATGAACAAGGAAGATTTATTTGTATCAATAAAAAAGTGCTTTTCTTCTCTTTGGAGCATTAGGGCA TTTTCATATAGAACTAATAATGGATATGATCATTTGAATGTTGGTATAGCAGTTGTTATTCAAGAAATGATAGAATCTGA TATTTCTGGAGTTATGTTTACATCTAACCCAATAACCGCTGAAAAGGAAATAATGATAGATGCATCTTATAATTTGGGAG AAGCCATAGTGTCAGGAAAAGTTACGCCAGATAATTATGTTCTTGATAAAAATGGAGAAGAAATAGCTTTCACTCTTGGA AGTAAGGAAATTTCAGTTGTATATTCAGATAAAGGAACTGTGGTTGTAAATAATTCTTCTGATATGAGAGAAAGAAGATG CCTTCATAATGAAAATTTAAGAGAATTATTTGATATGGCTTTAAAGATAGAAGGCTTATATAAAAAACCTATGGATATAG AATGGGCAATTAAAAATAAAAAAGTATATATTCTTCAAGCACGCCCAATTACAACTATAAAGAAAAAACAGAATAAAAAT ACCAGAAAGCAGTACTCACAAAAAGAAAAAAAGTATTTAAATAATATGATAGAACATTTTCCAGTTGCATGTTATCCACT AGATTATGAAATTGCAATGATACTTCAAAATATGAAATTTGAACTTTTTAAGGATGTAGGACTGAAAATAGAAAGTCCTA TATATATGGATAATATGGGTATAATCCATATGAACAAAATTAGGTGGAAAGTAAATCCAAAAATTTTACGCTTTTTTAAA GTTTATTATGAATATAAAAATAATGAAAAGAATGTTGAATATGGAGAAAATATTTTAGATTCTTGTAAAAAAGAGCTCAA AGTAATAGAACAAAATATAAATGAAAATAGTTATAAATTTTCTCTTCGTGATGTAGATAAATATATGAGTGATATTATTT CAATTCATGAAAGAATTTCTTTTGCAAGATTTAGATATTTTCTTTTCCCATCTGTTATTATTGGCGAAAAACTTGATAAA GAACTTAAAGCTATAAATCCAAATTATACAGAGTATGATCTCTTATCAGATTTAGATTATGTAACAGTAGATATGAATAA GGATATTGATATTTTATCAAAAACTTTATCAAGAAATGAAGAACTAATAAAGGATTTAATAAAAGGGGCAAGCTATGCAT TTATAAAAGAAAAATATGATTATGATTCAATAGAAATAAAAGAGTTTTTTAATAAATATGGTTGGAAGTCTAATTATTGT TGTATTCCATTCTCTTCATCTTCTTGGAATGAGGAACCAAATAGATTTATAAAACTTCTTAAAATAACCATTTCAGAGAA TAAAGAATATGAAGAAAATAAATCTGGAAAGTACATGGGTATATTGAAAGAGTTGGAGGAAAGTAAAGGTAAAAAAAGAT TTAAGAAAACAATGGGAGATATTACTTATTATAGACTAGCTCATGTTAGACGTGAGGAAAGTCAATATCTTTGGGAAACA GGATTTGGATTATTGAGGTTGCTTTTAAAACAAATAGAAGATTTGTTAAATAAAAAGAAAGAAGATTTGCTATATTTGTC TTTTCAAGAGTTAAGAAAAGTAATAGCTATTGGTAAGTTAGAGCATGAATATAGAGAATTAATTTCATATAGGAAAAATC ACCGTAAGGATGCTGAAAAACTTTGGGAGCAGATGGCATTAGATGCCTTTTACGATAATGAGAAGAATATTTTCAAGGGA ATAAGCGGAAGTTCGGGTGTAGTGAGAGGTAGAGTTTGTATTATAAAAAATCATACTGAATTTGATAAACTGAAAGAAGA TAATATTCTTATATGTCCATATACTGATCCTGAATGGACCCCTCTTTTCAAAATAGCAAAAGGAGTTGTTTCAGATACAG GTGGAGCTTTATCGCACGCAGCTATTGTTGCACGTGAATATAATATCCCAGCGGTATTGGGTGTTGGAAATGCCACAGTA AATTTATCAGATGGGGAATATATATTATTAGACGGAAATAATGGTATTGTAAAGCGATTATCAGAGGAAATAATATGA
Upstream 100 bases:
>100_bases GATGCTACAGTAGATCTTTATAAATCAATATCTCTAAATGAAATAGTTGAGAATATTGAAAAGACTAAAATTGATTTAGA AGATATAGTGGGGTGAAAAA
Downstream 100 bases:
>100_bases TAAAGAATATAGAAGATAAGCAGGGAAAATTTGAAGAACTTACTAAGACGCCAATTCCCAAATTAATATTGAAAATGGCT ATACCGTCTACAATGGCAAT
Product: putative phosphoenolpyruvate synthase
Products: AMP; phosphoenolpyruvate; phosphate
Alternate protein names: Putative PEP synthase; Pyruvate, water dikinase [H]
Number of amino acids: Translated: 825; Mature: 825
Protein sequence:
>825_residues MDTKNIIGIKDARENQIDIVGGKGANLGLMISYGIPVPDGFIVTANAYKNFLKSNGILEIIEQKISNIDKETLSIKGKTE EIRNLILKAEFPKDLKEDILSRFNKFKRPIHLAVRSSATAEDLPEASFAGQQETYLNIMNKEDLFVSIKKCFSSLWSIRA FSYRTNNGYDHLNVGIAVVIQEMIESDISGVMFTSNPITAEKEIMIDASYNLGEAIVSGKVTPDNYVLDKNGEEIAFTLG SKEISVVYSDKGTVVVNNSSDMRERRCLHNENLRELFDMALKIEGLYKKPMDIEWAIKNKKVYILQARPITTIKKKQNKN TRKQYSQKEKKYLNNMIEHFPVACYPLDYEIAMILQNMKFELFKDVGLKIESPIYMDNMGIIHMNKIRWKVNPKILRFFK VYYEYKNNEKNVEYGENILDSCKKELKVIEQNINENSYKFSLRDVDKYMSDIISIHERISFARFRYFLFPSVIIGEKLDK ELKAINPNYTEYDLLSDLDYVTVDMNKDIDILSKTLSRNEELIKDLIKGASYAFIKEKYDYDSIEIKEFFNKYGWKSNYC CIPFSSSSWNEEPNRFIKLLKITISENKEYEENKSGKYMGILKELEESKGKKRFKKTMGDITYYRLAHVRREESQYLWET GFGLLRLLLKQIEDLLNKKKEDLLYLSFQELRKVIAIGKLEHEYRELISYRKNHRKDAEKLWEQMALDAFYDNEKNIFKG ISGSSGVVRGRVCIIKNHTEFDKLKEDNILICPYTDPEWTPLFKIAKGVVSDTGGALSHAAIVAREYNIPAVLGVGNATV NLSDGEYILLDGNNGIVKRLSEEII
Sequences:
>Translated_825_residues MDTKNIIGIKDARENQIDIVGGKGANLGLMISYGIPVPDGFIVTANAYKNFLKSNGILEIIEQKISNIDKETLSIKGKTE EIRNLILKAEFPKDLKEDILSRFNKFKRPIHLAVRSSATAEDLPEASFAGQQETYLNIMNKEDLFVSIKKCFSSLWSIRA FSYRTNNGYDHLNVGIAVVIQEMIESDISGVMFTSNPITAEKEIMIDASYNLGEAIVSGKVTPDNYVLDKNGEEIAFTLG SKEISVVYSDKGTVVVNNSSDMRERRCLHNENLRELFDMALKIEGLYKKPMDIEWAIKNKKVYILQARPITTIKKKQNKN TRKQYSQKEKKYLNNMIEHFPVACYPLDYEIAMILQNMKFELFKDVGLKIESPIYMDNMGIIHMNKIRWKVNPKILRFFK VYYEYKNNEKNVEYGENILDSCKKELKVIEQNINENSYKFSLRDVDKYMSDIISIHERISFARFRYFLFPSVIIGEKLDK ELKAINPNYTEYDLLSDLDYVTVDMNKDIDILSKTLSRNEELIKDLIKGASYAFIKEKYDYDSIEIKEFFNKYGWKSNYC CIPFSSSSWNEEPNRFIKLLKITISENKEYEENKSGKYMGILKELEESKGKKRFKKTMGDITYYRLAHVRREESQYLWET GFGLLRLLLKQIEDLLNKKKEDLLYLSFQELRKVIAIGKLEHEYRELISYRKNHRKDAEKLWEQMALDAFYDNEKNIFKG ISGSSGVVRGRVCIIKNHTEFDKLKEDNILICPYTDPEWTPLFKIAKGVVSDTGGALSHAAIVAREYNIPAVLGVGNATV NLSDGEYILLDGNNGIVKRLSEEII >Mature_825_residues MDTKNIIGIKDARENQIDIVGGKGANLGLMISYGIPVPDGFIVTANAYKNFLKSNGILEIIEQKISNIDKETLSIKGKTE EIRNLILKAEFPKDLKEDILSRFNKFKRPIHLAVRSSATAEDLPEASFAGQQETYLNIMNKEDLFVSIKKCFSSLWSIRA FSYRTNNGYDHLNVGIAVVIQEMIESDISGVMFTSNPITAEKEIMIDASYNLGEAIVSGKVTPDNYVLDKNGEEIAFTLG SKEISVVYSDKGTVVVNNSSDMRERRCLHNENLRELFDMALKIEGLYKKPMDIEWAIKNKKVYILQARPITTIKKKQNKN TRKQYSQKEKKYLNNMIEHFPVACYPLDYEIAMILQNMKFELFKDVGLKIESPIYMDNMGIIHMNKIRWKVNPKILRFFK VYYEYKNNEKNVEYGENILDSCKKELKVIEQNINENSYKFSLRDVDKYMSDIISIHERISFARFRYFLFPSVIIGEKLDK ELKAINPNYTEYDLLSDLDYVTVDMNKDIDILSKTLSRNEELIKDLIKGASYAFIKEKYDYDSIEIKEFFNKYGWKSNYC CIPFSSSSWNEEPNRFIKLLKITISENKEYEENKSGKYMGILKELEESKGKKRFKKTMGDITYYRLAHVRREESQYLWET GFGLLRLLLKQIEDLLNKKKEDLLYLSFQELRKVIAIGKLEHEYRELISYRKNHRKDAEKLWEQMALDAFYDNEKNIFKG ISGSSGVVRGRVCIIKNHTEFDKLKEDNILICPYTDPEWTPLFKIAKGVVSDTGGALSHAAIVAREYNIPAVLGVGNATV NLSDGEYILLDGNNGIVKRLSEEII
Specific function: Might catalyze the phosphorylation of pyruvate to phosphoenolpyruvate (Potential) [H]
COG id: COG0574
COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the PEP-utilizing enzyme family [H]
Homologues:
Organism=Escherichia coli, GI1787994, Length=356, Percent_Identity=35.9550561797753, Blast_Score=200, Evalue=4e-52, Organism=Caenorhabditis elegans, GI17564524, Length=363, Percent_Identity=31.6804407713499, Blast_Score=131, Evalue=2e-30,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR013815 - InterPro: IPR013816 - InterPro: IPR008279 - InterPro: IPR002192 [H]
Pfam domain/function: PF00391 PEP-utilizers; PF01326 PPDK_N [H]
EC number: 2.7.9.2
Molecular weight: Translated: 95444; Mature: 95444
Theoretical pI: Translated: 8.13; Mature: 8.13
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.0 %Cys (Translated Protein) 2.4 %Met (Translated Protein) 3.4 %Cys+Met (Translated Protein) 1.0 %Cys (Mature Protein) 2.4 %Met (Mature Protein) 3.4 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MDTKNIIGIKDARENQIDIVGGKGANLGLMISYGIPVPDGFIVTANAYKNFLKSNGILEI CCCCCEEECCCCCCCCEEEECCCCCCEEEEEEECCCCCCCEEEECHHHHHHHHCCCHHHH IEQKISNIDKETLSIKGKTEEIRNLILKAEFPKDLKEDILSRFNKFKRPIHLAVRSSATA HHHHHHCCCCHHEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCH EDLPEASFAGQQETYLNIMNKEDLFVSIKKCFSSLWSIRAFSYRTNNGYDHLNVGIAVVI HHCCCCCCCCCCHHHHEECCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHH QEMIESDISGVMFTSNPITAEKEIMIDASYNLGEAIVSGKVTPDNYVLDKNGEEIAFTLG HHHHHHCCCCEEEECCCCCCCCEEEEECCCCCCHHHHCCCCCCCCEEECCCCCEEEEEEC SKEISVVYSDKGTVVVNNSSDMRERRCLHNENLRELFDMALKIEGLYKKPMDIEWAIKNK CCEEEEEEECCCEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECC KVYILQARPITTIKKKQNKNTRKQYSQKEKKYLNNMIEHFPVACYPLDYEIAMILQNMKF EEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHH ELFKDVGLKIESPIYMDNMGIIHMNKIRWKVNPKILRFFKVYYEYKNNEKNVEYGENILD HHHHHCCCEECCCEEECCCCEEEEEEEEEEECHHHHHHHHHHHHHCCCCCCCHHHHHHHH SCKKELKVIEQNINENSYKFSLRDVDKYMSDIISIHERISFARFRYFLFPSVIIGEKLDK HHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH ELKAINPNYTEYDLLSDLDYVTVDMNKDIDILSKTLSRNEELIKDLIKGASYAFIKEKYD HHHHCCCCCCHHHHHCCCCEEEEECCCCHHHHHHHHHCCHHHHHHHHCCCCEEEEHHCCC YDSIEIKEFFNKYGWKSNYCCIPFSSSSWNEEPNRFIKLLKITISENKEYEENKSGKYMG CCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHEEEECCCCCCCCCCCCCHHH ILKELEESKGKKRFKKTMGDITYYRLAHVRREESQYLWETGFGLLRLLLKQIEDLLNKKK HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH EDLLYLSFQELRKVIAIGKLEHEYRELISYRKNHRKDAEKLWEQMALDAFYDNEKNIFKG HHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHCC ISGSSGVVRGRVCIIKNHTEFDKLKEDNILICPYTDPEWTPLFKIAKGVVSDTGGALSHA CCCCCCEEEEEEEEEECCCHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCHHHHH AIVAREYNIPAVLGVGNATVNLSDGEYILLDGNNGIVKRLSEEII HHEEEECCCCEEEECCCEEEEECCCCEEEEECCCHHHHHHHHHCC >Mature Secondary Structure MDTKNIIGIKDARENQIDIVGGKGANLGLMISYGIPVPDGFIVTANAYKNFLKSNGILEI CCCCCEEECCCCCCCCEEEECCCCCCEEEEEEECCCCCCCEEEECHHHHHHHHCCCHHHH IEQKISNIDKETLSIKGKTEEIRNLILKAEFPKDLKEDILSRFNKFKRPIHLAVRSSATA HHHHHHCCCCHHEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCH EDLPEASFAGQQETYLNIMNKEDLFVSIKKCFSSLWSIRAFSYRTNNGYDHLNVGIAVVI HHCCCCCCCCCCHHHHEECCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHH QEMIESDISGVMFTSNPITAEKEIMIDASYNLGEAIVSGKVTPDNYVLDKNGEEIAFTLG HHHHHHCCCCEEEECCCCCCCCEEEEECCCCCCHHHHCCCCCCCCEEECCCCCEEEEEEC SKEISVVYSDKGTVVVNNSSDMRERRCLHNENLRELFDMALKIEGLYKKPMDIEWAIKNK CCEEEEEEECCCEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECC KVYILQARPITTIKKKQNKNTRKQYSQKEKKYLNNMIEHFPVACYPLDYEIAMILQNMKF EEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHH ELFKDVGLKIESPIYMDNMGIIHMNKIRWKVNPKILRFFKVYYEYKNNEKNVEYGENILD HHHHHCCCEECCCEEECCCCEEEEEEEEEEECHHHHHHHHHHHHHCCCCCCCHHHHHHHH SCKKELKVIEQNINENSYKFSLRDVDKYMSDIISIHERISFARFRYFLFPSVIIGEKLDK HHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH ELKAINPNYTEYDLLSDLDYVTVDMNKDIDILSKTLSRNEELIKDLIKGASYAFIKEKYD HHHHCCCCCCHHHHHCCCCEEEEECCCCHHHHHHHHHCCHHHHHHHHCCCCEEEEHHCCC YDSIEIKEFFNKYGWKSNYCCIPFSSSSWNEEPNRFIKLLKITISENKEYEENKSGKYMG CCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHEEEECCCCCCCCCCCCCHHH ILKELEESKGKKRFKKTMGDITYYRLAHVRREESQYLWETGFGLLRLLLKQIEDLLNKKK HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH EDLLYLSFQELRKVIAIGKLEHEYRELISYRKNHRKDAEKLWEQMALDAFYDNEKNIFKG HHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHCC ISGSSGVVRGRVCIIKNHTEFDKLKEDNILICPYTDPEWTPLFKIAKGVVSDTGGALSHA CCCCCCEEEEEEEEEECCCHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCHHHHH AIVAREYNIPAVLGVGNATVNLSDGEYILLDGNNGIVKRLSEEII HHEEEECCCCEEEECCCEEEEECCCCEEEEECCCHHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: Mg2+; Mn2+ [C]
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): 10.5 {phosphate}} 0.083 {pyruvate}} 0.028 {ATP}} [C]
Substrates: ATP; pyruvate; H2O
Specific reaction: ATP + pyruvate + H2O = AMP + phosphoenolpyruvate + phosphate
General reaction: Phospho group transfer [C]
Inhibitor: 2-Oxoglutarate; 3-Phosphoglyceraldehyde; 5'-Adenylyl methylen ediphosphonate; ADP; ADP glucose; AMP; ATP; Ca2+; F-; Iodoacetate; Malate; Mg2+; Mn2+; Oxalacetate; PCMB; Phosphoenolpyruvate; Sulfhydryl reagents [C]
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9384377; 7704256 [H]