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The map label for this gene is dosC [H]

Identifier: 170756668

GI number: 170756668

Start: 2153985

End: 2154533

Strand: Reverse

Name: dosC [H]

Synonym: CLD_2617

Alternate gene names: 170756668

Gene position: 2154533-2153985 (Counterclockwise)

Preceding gene: 170755542

Following gene: 170755820

Centisome position: 54.43

GC content: 24.77

Gene sequence:

>549_bases
ATGAATTATAATCATATGTCCAGGAGTGAATTGATAGAAGAATTAAATAGAAAAGATAAAATAATAAAGAGGATGAAATA
TATTTTTGGAAAAGATCCTGTTACATCCGTGCTTAATAGAAAATTAGGTTTAGAGAAACTTCATAGAGAAATCAAGCTAG
CAAAAATTAATAAAGGTAATTTAATTATAACCTTCGCGGATGTAAATAATCTTAAATATATAAATGATAATTATGGCCAT
GATGTCGGAGATTATGTATTAGAAACTCTATGTAGTATAATAAAAAACAATATAGGAAAAATGGATTTTATTTTTAGATA
TGGGGGAGATGAATTTATAATTGTATTTCTCAAATCTAATATGGATGATGCAAATAAAATATTGTCTAGAATTCAAAAGG
AAATAGATGAATTAGGAAAAAATCTAGAATATAAAATGGGGTTAAGTTATGGTTTAGTAGAGTACAATAAAAATACAAAT
AAAACCATAGAAGATTTAATAAGAATAGCAGATTGTAGGATGTATGAGAATAAAAGAGAAATTGGATAA

Upstream 100 bases:

>100_bases
TGAAAAAAATTTTAAATCATCTCTAAAATGTACAAATTTCGACTGGGTAATATTGTAAAATAATTATGAGTAATATTTAA
ATTAGTAGGAGTGTTTCTTT

Downstream 100 bases:

>100_bases
GACTTTGAAAATTTGTATAATATCATTATACAAATTTTTTTATTTTCGATGTTAAAATTAAATCTATTTTATATTGAGGT
AAATTATATCTTTAAAAACA

Product: GGDEF domain-containing protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MNYNHMSRSELIEELNRKDKIIKRMKYIFGKDPVTSVLNRKLGLEKLHREIKLAKINKGNLIITFADVNNLKYINDNYGH
DVGDYVLETLCSIIKNNIGKMDFIFRYGGDEFIIVFLKSNMDDANKILSRIQKEIDELGKNLEYKMGLSYGLVEYNKNTN
KTIEDLIRIADCRMYENKREIG

Sequences:

>Translated_182_residues
MNYNHMSRSELIEELNRKDKIIKRMKYIFGKDPVTSVLNRKLGLEKLHREIKLAKINKGNLIITFADVNNLKYINDNYGH
DVGDYVLETLCSIIKNNIGKMDFIFRYGGDEFIIVFLKSNMDDANKILSRIQKEIDELGKNLEYKMGLSYGLVEYNKNTN
KTIEDLIRIADCRMYENKREIG
>Mature_182_residues
MNYNHMSRSELIEELNRKDKIIKRMKYIFGKDPVTSVLNRKLGLEKLHREIKLAKINKGNLIITFADVNNLKYINDNYGH
DVGDYVLETLCSIIKNNIGKMDFIFRYGGDEFIIVFLKSNMDDANKILSRIQKEIDELGKNLEYKMGLSYGLVEYNKNTN
KTIEDLIRIADCRMYENKREIG

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=174, Percent_Identity=33.3333333333333, Blast_Score=90, Evalue=9e-20,
Organism=Escherichia coli, GI87081974, Length=149, Percent_Identity=31.5436241610738, Blast_Score=80, Evalue=7e-17,
Organism=Escherichia coli, GI87081977, Length=183, Percent_Identity=30.6010928961749, Blast_Score=76, Evalue=1e-15,
Organism=Escherichia coli, GI1787262, Length=185, Percent_Identity=28.6486486486486, Blast_Score=74, Evalue=4e-15,
Organism=Escherichia coli, GI87082007, Length=152, Percent_Identity=26.3157894736842, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI87081881, Length=170, Percent_Identity=23.5294117647059, Blast_Score=66, Evalue=1e-12,
Organism=Escherichia coli, GI1788381, Length=173, Percent_Identity=26.5895953757225, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI1787816, Length=155, Percent_Identity=29.0322580645161, Blast_Score=64, Evalue=5e-12,
Organism=Escherichia coli, GI1786584, Length=160, Percent_Identity=23.75, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1787056, Length=147, Percent_Identity=31.2925170068027, Blast_Score=60, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 21324; Mature: 21324

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYNHMSRSELIEELNRKDKIIKRMKYIFGKDPVTSVLNRKLGLEKLHREIKLAKINKGN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHEEECCCC
LIITFADVNNLKYINDNYGHDVGDYVLETLCSIIKNNIGKMDFIFRYGGDEFIIVFLKSN
EEEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEEEEECC
MDDANKILSRIQKEIDELGKNLEYKMGLSYGLVEYNKNTNKTIEDLIRIADCRMYENKRE
CCHHHHHHHHHHHHHHHHCCCCEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCC
IG
CC
>Mature Secondary Structure
MNYNHMSRSELIEELNRKDKIIKRMKYIFGKDPVTSVLNRKLGLEKLHREIKLAKINKGN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHEEECCCC
LIITFADVNNLKYINDNYGHDVGDYVLETLCSIIKNNIGKMDFIFRYGGDEFIIVFLKSN
EEEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEEEEECC
MDDANKILSRIQKEIDELGKNLEYKMGLSYGLVEYNKNTNKTIEDLIRIADCRMYENKRE
CCHHHHHHHHHHHHHHHHCCCCEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCC
IG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]