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The map label for this gene is ispD

Identifier: 170756659

GI number: 170756659

Start: 3804074

End: 3804763

Strand: Reverse

Name: ispD

Synonym: CLD_0997

Alternate gene names: 170756659

Gene position: 3804763-3804074 (Counterclockwise)

Preceding gene: 170757652

Following gene: 170756076

Centisome position: 96.12

GC content: 28.55

Gene sequence:

>690_bases
ATGAGTAAAAATTCTGTTATTATAGTTGCTGCCGGAAAAGGTAAAAGAATGAATAGCAGTATAAGTAAGCAGTTTTTGCA
GATAAAAAACAAACCTATTCTTTATTATACTTTAAGCAAATTTTCAACCCATGAATCTATAGATGAAATAGTGCTAGTTA
CCTTAGAAGATAAAATAGAAGTTTGCGGTGATATTATAGATAAATATAATATCAATAAGGTATCTAAAATAGTTCCAGGA
GGTAAGGAAAGACAAGATTCTGTTTATAATGGACTAAAGGCTGTATCAAAGGATTGTGAGGTAGTATTAATTCATGATGC
AGCAAGACCTTTTGTTACTAGTGATATTATAGAGAATGGTATAAGATATACGAATCAATATGGGGCTGCTGCTTGTGGGG
TAATTCCAAAAGATACCATAAAAATAAAAAGTGAAAAAGGATTTGCAATAGATACTCCTAACAGAGAGGATCTATTTATA
GTTCAAACTCCTCAGTGTTTTAATTACAATATAATATTAGATTGTCATGAAAAGTTAAAAAAGCATAATAAAAAGGTAAC
AGATGATACTATGGTTTTAGAGAATTACGGTAAATCAGTATATTTATATGAAGGAAACTATAGTAATATAAAAATAACAA
CTCCTGAAGATTTAATATTAGGAGAACAAATATTAGAAAAGCTAACTTAG

Upstream 100 bases:

>100_bases
AAAAAACGTATAGGGCAAAATTTAGACGTAGTAGTAACCTCTGTTTTACAAACAGCGGCAGGAAGAATGATTTTTGCTAA
GAGAAAAGGGGAGTCTTAGC

Downstream 100 bases:

>100_bases
CAATAAAACTCGTCCTAATAAGGGGCGAGTTTTGTTTATATATTGCATGTTTAAAATTTCTATCGAGGATATTTTTAAAT
ATAATGAATAATTTGTTAGG

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT [H]

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MSKNSVIIVAAGKGKRMNSSISKQFLQIKNKPILYYTLSKFSTHESIDEIVLVTLEDKIEVCGDIIDKYNINKVSKIVPG
GKERQDSVYNGLKAVSKDCEVVLIHDAARPFVTSDIIENGIRYTNQYGAAACGVIPKDTIKIKSEKGFAIDTPNREDLFI
VQTPQCFNYNIILDCHEKLKKHNKKVTDDTMVLENYGKSVYLYEGNYSNIKITTPEDLILGEQILEKLT

Sequences:

>Translated_229_residues
MSKNSVIIVAAGKGKRMNSSISKQFLQIKNKPILYYTLSKFSTHESIDEIVLVTLEDKIEVCGDIIDKYNINKVSKIVPG
GKERQDSVYNGLKAVSKDCEVVLIHDAARPFVTSDIIENGIRYTNQYGAAACGVIPKDTIKIKSEKGFAIDTPNREDLFI
VQTPQCFNYNIILDCHEKLKKHNKKVTDDTMVLENYGKSVYLYEGNYSNIKITTPEDLILGEQILEKLT
>Mature_228_residues
SKNSVIIVAAGKGKRMNSSISKQFLQIKNKPILYYTLSKFSTHESIDEIVLVTLEDKIEVCGDIIDKYNINKVSKIVPGG
KERQDSVYNGLKAVSKDCEVVLIHDAARPFVTSDIIENGIRYTNQYGAAACGVIPKDTIKIKSEKGFAIDTPNREDLFIV
QTPQCFNYNIILDCHEKLKKHNKKVTDDTMVLENYGKSVYLYEGNYSNIKITTPEDLILGEQILEKLT

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP) [H]

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family [H]

Homologues:

Organism=Homo sapiens, GI157412259, Length=233, Percent_Identity=27.8969957081545, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI157671913, Length=134, Percent_Identity=33.5820895522388, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1789104, Length=227, Percent_Identity=32.5991189427313, Blast_Score=108, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294 [H]

Pfam domain/function: PF01128 IspD [H]

EC number: =2.7.7.60 [H]

Molecular weight: Translated: 25772; Mature: 25641

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKNSVIIVAAGKGKRMNSSISKQFLQIKNKPILYYTLSKFSTHESIDEIVLVTLEDKIE
CCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEEEECHHHH
VCGDIIDKYNINKVSKIVPGGKERQDSVYNGLKAVSKDCEVVLIHDAARPFVTSDIIENG
HHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCC
IRYTNQYGAAACGVIPKDTIKIKSEKGFAIDTPNREDLFIVQTPQCFNYNIILDCHEKLK
HHCCCCCCCEECCCCCCCEEEEECCCCEEEECCCCCCEEEEECCCEECEEEEEEHHHHHH
KHNKKVTDDTMVLENYGKSVYLYEGNYSNIKITTPEDLILGEQILEKLT
HCCCCCCCHHHHHHHCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKNSVIIVAAGKGKRMNSSISKQFLQIKNKPILYYTLSKFSTHESIDEIVLVTLEDKIE
CCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEEEECHHHH
VCGDIIDKYNINKVSKIVPGGKERQDSVYNGLKAVSKDCEVVLIHDAARPFVTSDIIENG
HHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCC
IRYTNQYGAAACGVIPKDTIKIKSEKGFAIDTPNREDLFIVQTPQCFNYNIILDCHEKLK
HHCCCCCCCEECCCCCCCEEEEECCCCEEEECCCCCCEEEEECCCEECEEEEEEHHHHHH
KHNKKVTDDTMVLENYGKSVYLYEGNYSNIKITTPEDLILGEQILEKLT
HCCCCCCCHHHHHHHCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA