Click here to switch to the map view.

The map label for this gene is epsE [H]

Identifier: 170756610

GI number: 170756610

Start: 2036271

End: 2037800

Strand: Reverse

Name: epsE [H]

Synonym: CLD_2718

Alternate gene names: 170756610

Gene position: 2037800-2036271 (Counterclockwise)

Preceding gene: 170755288

Following gene: 170757657

Centisome position: 51.48

GC content: 26.6

Gene sequence:

>1530_bases
ATGAGCTTTTCTATGAATTATTTAGATTTAAAGGAGACTGTACAAAACTATGAAGAATTAAGAGGGGGAAAGCAGTGTGA
AAATATAGACTTTAGTACTTTAAAAATAGAATCTTCTCTGCTAAAATTGCTGCCAGAGCATATTTGTAGAAAATATAATA
TATTACCCCTAAAAATAATTGGCGAACAATTGTATATAGCATCTTCAAATTACTTAAAAGAAGATGCTTTATATAAAATA
TCCTTTATTACGGGTAAAAATATAAAAATAGTTCTTTGTAATAAAGAAGATATTTTAGGGGCTATAAAGAGATTTTATAC
AAACTATGAAGGAAAATATAATGTTAAAGGAATAAAGGAAGAAAAAGCTATATCCTATGAAGAAAATAAAGAAAAAAATT
TAGAAGGCCCTATAATAAAGTTAACAGATTCTATAATAGAAGAGGCTGTTTGGAGAAAGGCTAGTGATATACATATAGAA
CCCTTCAAAGATTTTGCTTTAATAAGATTTAGAATAGATGGCATTTTAATAGAATTCAAAAAAATATCAAAAAAAATTTA
CACTTCTATTTGCACTCGTATAAAAATAATAAGTTATATGAACATAGCAGAAAAAAACATTCCTCAGGATGGTAAAATAC
AAAACAATTATAGAGAAAATAATGATTTTAGAGTATCTACATTGCCCACAGTATATGGCGAAAAACTAGTGATAAGAATA
CTATATAAAAATGAAAAAATAGCAGATTTAAAATCTTTAGGATTTTTAAAAGGAGATAGAAAAAATATAGAAAAAATGCT
TAAAAAACCTAATGGATTAATTTTAATTACAGGGCCTACAGGCAGTGGTAAATCTACAACTTTATATTCTATGCTTCAAT
ATATAAACAATAAAGAAAAAAACATAATAACCATTGAAGAGCCAGTAGAATATACTATAGATGGTATAAACCAAGTTAAT
GTAGATTATAAAAGGGAACTTACTTTTCTAAAAGGTTTAAAATCAATACTTAGACAGGACCCGGATATAATAATGGTAGG
AGAAATTAGAGATGAAGAAACAGCTAAAGTAGCGGTAAGAGCCTCTATAACAGGACATTTAGTTTTATCTACTCTACATA
CTAATGGAGCCTTAGAATCTATAATAAGGCTTTTGGATATGAAAATAGAACCCTATATTCTAAGTGATGCCTTAAGAGGA
ATTATTTCACAAAGATTGGTGAGAAAGATATGTCCTTATTGCAAAGAAGCATATATACCTTCAACGGAGGAAAAGAACTT
TGTAAATTTAAAAGAAGAGTTTTTACTATATAAGGGTGAAGGATGCAATAAATGCAATAATACAGGTTATATAGGTAGAA
GCGTTCTTTATGAATATATAAATATTGATAAAGAAAAAAAGGACTTAATTAAAAACATAGTAAAAAACAATAATGAAGAA
ATTTTTTTAGAAAATAGCTTAAAAGAAAATGTTAATAAAGCATTAATACAAGGAAGGACATCCTTTAGTGAAATCCAAAG
AATAATATAA

Upstream 100 bases:

>100_bases
TTTAAAACATAGTCAATATATAAAATAAAGTGTTTACTTTTTATATATGTGTCTATAATATAGATAAATTTTCAAATTTT
AATATAGGAGAGAATTAAAT

Downstream 100 bases:

>100_bases
ACAGAATTTTTAGTAACTAAACATCCTTACTAAAGAAACAACTAGATTGCTTTAAGATTCATTTTCAGTATATATTTCTA
AGAAAATGTCTTTTAAACTT

Product: general secretion pathway protein E

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MSFSMNYLDLKETVQNYEELRGGKQCENIDFSTLKIESSLLKLLPEHICRKYNILPLKIIGEQLYIASSNYLKEDALYKI
SFITGKNIKIVLCNKEDILGAIKRFYTNYEGKYNVKGIKEEKAISYEENKEKNLEGPIIKLTDSIIEEAVWRKASDIHIE
PFKDFALIRFRIDGILIEFKKISKKIYTSICTRIKIISYMNIAEKNIPQDGKIQNNYRENNDFRVSTLPTVYGEKLVIRI
LYKNEKIADLKSLGFLKGDRKNIEKMLKKPNGLILITGPTGSGKSTTLYSMLQYINNKEKNIITIEEPVEYTIDGINQVN
VDYKRELTFLKGLKSILRQDPDIIMVGEIRDEETAKVAVRASITGHLVLSTLHTNGALESIIRLLDMKIEPYILSDALRG
IISQRLVRKICPYCKEAYIPSTEEKNFVNLKEEFLLYKGEGCNKCNNTGYIGRSVLYEYINIDKEKKDLIKNIVKNNNEE
IFLENSLKENVNKALIQGRTSFSEIQRII

Sequences:

>Translated_509_residues
MSFSMNYLDLKETVQNYEELRGGKQCENIDFSTLKIESSLLKLLPEHICRKYNILPLKIIGEQLYIASSNYLKEDALYKI
SFITGKNIKIVLCNKEDILGAIKRFYTNYEGKYNVKGIKEEKAISYEENKEKNLEGPIIKLTDSIIEEAVWRKASDIHIE
PFKDFALIRFRIDGILIEFKKISKKIYTSICTRIKIISYMNIAEKNIPQDGKIQNNYRENNDFRVSTLPTVYGEKLVIRI
LYKNEKIADLKSLGFLKGDRKNIEKMLKKPNGLILITGPTGSGKSTTLYSMLQYINNKEKNIITIEEPVEYTIDGINQVN
VDYKRELTFLKGLKSILRQDPDIIMVGEIRDEETAKVAVRASITGHLVLSTLHTNGALESIIRLLDMKIEPYILSDALRG
IISQRLVRKICPYCKEAYIPSTEEKNFVNLKEEFLLYKGEGCNKCNNTGYIGRSVLYEYINIDKEKKDLIKNIVKNNNEE
IFLENSLKENVNKALIQGRTSFSEIQRII
>Mature_508_residues
SFSMNYLDLKETVQNYEELRGGKQCENIDFSTLKIESSLLKLLPEHICRKYNILPLKIIGEQLYIASSNYLKEDALYKIS
FITGKNIKIVLCNKEDILGAIKRFYTNYEGKYNVKGIKEEKAISYEENKEKNLEGPIIKLTDSIIEEAVWRKASDIHIEP
FKDFALIRFRIDGILIEFKKISKKIYTSICTRIKIISYMNIAEKNIPQDGKIQNNYRENNDFRVSTLPTVYGEKLVIRIL
YKNEKIADLKSLGFLKGDRKNIEKMLKKPNGLILITGPTGSGKSTTLYSMLQYINNKEKNIITIEEPVEYTIDGINQVNV
DYKRELTFLKGLKSILRQDPDIIMVGEIRDEETAKVAVRASITGHLVLSTLHTNGALESIIRLLDMKIEPYILSDALRGI
ISQRLVRKICPYCKEAYIPSTEEKNFVNLKEEFLLYKGEGCNKCNNTGYIGRSVLYEYINIDKEKKDLIKNIVKNNNEEI
FLENSLKENVNKALIQGRTSFSEIQRII

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=384, Percent_Identity=44.0104166666667, Blast_Score=348, Evalue=5e-97,
Organism=Escherichia coli, GI1786296, Length=476, Percent_Identity=34.453781512605, Blast_Score=297, Evalue=1e-81,
Organism=Escherichia coli, GI87082188, Length=200, Percent_Identity=41, Blast_Score=138, Evalue=7e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 58689; Mature: 58558

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFSMNYLDLKETVQNYEELRGGKQCENIDFSTLKIESSLLKLLPEHICRKYNILPLKII
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCEEEEEE
GEQLYIASSNYLKEDALYKISFITGKNIKIVLCNKEDILGAIKRFYTNYEGKYNVKGIKE
CCEEEEECCCCCCCCCEEEEEEEECCCEEEEEECCHHHHHHHHHHHCCCCCEEECCCCCH
EKAISYEENKEKNLEGPIIKLTDSIIEEAVWRKASDIHIEPFKDFALIRFRIDGILIEFK
HHCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCEEEHHH
KISKKIYTSICTRIKIISYMNIAEKNIPQDGKIQNNYRENNDFRVSTLPTVYGEKLVIRI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCHHCCEEEEEE
LYKNEKIADLKSLGFLKGDRKNIEKMLKKPNGLILITGPTGSGKSTTLYSMLQYINNKEK
EECCCCHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCC
NIITIEEPVEYTIDGINQVNVDYKRELTFLKGLKSILRQDPDIIMVGEIRDEETAKVAVR
CEEEECCCCHHHHCCCEECCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEE
ASITGHLVLSTLHTNGALESIIRLLDMKIEPYILSDALRGIISQRLVRKICPYCKEAYIP
EECCEEEEEEEHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
STEEKNFVNLKEEFLLYKGEGCNKCNNTGYIGRSVLYEYINIDKEKKDLIKNIVKNNNEE
CCCCCCCCCCHHHEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCE
IFLENSLKENVNKALIQGRTSFSEIQRII
EEEECHHHHHHHHHHHHCCCCHHHHHHCC
>Mature Secondary Structure 
SFSMNYLDLKETVQNYEELRGGKQCENIDFSTLKIESSLLKLLPEHICRKYNILPLKII
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCEEEEEE
GEQLYIASSNYLKEDALYKISFITGKNIKIVLCNKEDILGAIKRFYTNYEGKYNVKGIKE
CCEEEEECCCCCCCCCEEEEEEEECCCEEEEEECCHHHHHHHHHHHCCCCCEEECCCCCH
EKAISYEENKEKNLEGPIIKLTDSIIEEAVWRKASDIHIEPFKDFALIRFRIDGILIEFK
HHCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCEEEHHH
KISKKIYTSICTRIKIISYMNIAEKNIPQDGKIQNNYRENNDFRVSTLPTVYGEKLVIRI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCHHCCEEEEEE
LYKNEKIADLKSLGFLKGDRKNIEKMLKKPNGLILITGPTGSGKSTTLYSMLQYINNKEK
EECCCCHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCC
NIITIEEPVEYTIDGINQVNVDYKRELTFLKGLKSILRQDPDIIMVGEIRDEETAKVAVR
CEEEECCCCHHHHCCCEECCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEE
ASITGHLVLSTLHTNGALESIIRLLDMKIEPYILSDALRGIISQRLVRKICPYCKEAYIP
EECCEEEEEEEHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
STEEKNFVNLKEEFLLYKGEGCNKCNNTGYIGRSVLYEYINIDKEKKDLIKNIVKNNNEE
CCCCCCCCCCHHHEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCE
IFLENSLKENVNKALIQGRTSFSEIQRII
EEEECHHHHHHHHHHHHCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]