BacMap

Definition	Clostridium botulinum B1 str. Okra, complete genome.
Accession	NC_010516
Length	3,958,233

Click here to switch to the map view.

The map label for this gene is steT [H]

Identifier: 170756526

GI number: 170756526

Start: 1539168

End: 1540487

Strand: Direct

Name: steT [H]

Synonym: CLD_3162

Alternate gene names: 170756526

Gene position: 1539168-1540487 (Clockwise)

Preceding gene: 170757593

Following gene: 170755862

Centisome position: 38.89

GC content: 30.76

Gene sequence:

>1320_bases
ATGAAAAATGCTAAAGAAGAAAATTTGCAAAAAAACCTAGGATTATTTGATGCTATAGCTATAGTTATAGGTATGGTTAT
AGGATCAGGTATATTTTTTAAGCCAGCTATAGTCTTTAAAAATGCTGGTTCACCAATGTTAGGGATATTGGCTTGGTTAG
TTGGAGGCTTAATTACTATAGCTTCTGGTCTTACAATAGCAGAAATTGCTGCAGCTATTCCTAAGACTGGGGGAATATTT
GTTTATATTAAAGAATTATATGGAGAAAAACTTGCATTTTTACTAGGATGGGTCCAATCAATTGTATATATACCAGGGTC
AGCGGCAGCTTTATCCATAATACTTTCTATACAGATTACTAGTTTTATACCTTTAACAGCTATTGAACAAAAAGTTTTAG
CTATAGTATTTATAATATTCATCATGATTTTAAATATTATATCTACTAAATTAGTAAGTAAAATGCAAGGGATAATAAGT
ATTGCTAAACTAATACCTATCATCGCTATAATAGCTTTAGGATTTATAAAGGGAACAGCAAAAGGGGATATACTAAATAT
TAGTTCTTCAGGTACTGTATCTGGGTTTGGAGCTGCACTTTTAGGAACTTTATGGGCTTATGATGGATGGATAAGTGTAG
GTAATATGGCAGGAGAACTTAAAAATCCTAAAAAAGATTTGCCAAGATCTATAATAATAGGTTTAGGTACTACTATAGTA
GTGTATGTACTTATAAATTTAGCTCTAATTAATATAATACCAATGGGTGCAATAATAACATCTGAAAAACCAGCTTCAGA
TGCAGCAGTAGTATTATTAGGAAGCACAGGAGCAAAAATCATTTCAGCAGGAATATTAATTTCTATATTCGGAGCCTTAA
ATGGATATTTGATGACAGGAGTTAGAATACCATTAGCTATGTCTCAGGATAGGAGCATACCTTTTAGTAAGTATTTTAGT
AAAGTTCATGAAAAATCAGGAACACCAGTTAATGCCTTTATATTTGAAACTGTATTAGCTTGTTTATATGTATTATCAGG
ATCATTCAATGTACTAACGGACTTAGTGATGTTTATATTATGGCTATTCTTTACTATGGCAGTAGCAGGCATATTTGTTT
TAAGAACTAGATTTAAACATTTGGATAGACCCTATAAAGTGCCCTTATATCCATTAGTACCTCTTATAGGTATAATAGGA
AGTGGCTATATAATAATAAGTACATTAATAACAAATACTTTCTATGCTCTTTGTGGTATAGGAATAACCTTATTAGGTTT
ACCCGTTTATAGCTATATAAAAAAGAAGAATAAAGTATAA

Upstream 100 bases:

>100_bases
TTATATTTTTAATTTAAGATTATAGACTATAGTAGTTTATGGATAAAATTTTTCAGCAAAGTAATTTGATACTACATACT
AAAGAAAGGGAAGAAATGAT

Downstream 100 bases:

>100_bases
GCATTAGCTCACCATTATATATATTTTGATTTAATATATAAAGAAAAGCAGATTATAAAAAGTTATGTAACTTAGAATCT
TAAAGGATTCTAGAATTTTA

Product: amino acid permease family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MKNAKEENLQKNLGLFDAIAIVIGMVIGSGIFFKPAIVFKNAGSPMLGILAWLVGGLITIASGLTIAEIAAAIPKTGGIF
VYIKELYGEKLAFLLGWVQSIVYIPGSAAALSIILSIQITSFIPLTAIEQKVLAIVFIIFIMILNIISTKLVSKMQGIIS
IAKLIPIIAIIALGFIKGTAKGDILNISSSGTVSGFGAALLGTLWAYDGWISVGNMAGELKNPKKDLPRSIIIGLGTTIV
VYVLINLALINIIPMGAIITSEKPASDAAVVLLGSTGAKIISAGILISIFGALNGYLMTGVRIPLAMSQDRSIPFSKYFS
KVHEKSGTPVNAFIFETVLACLYVLSGSFNVLTDLVMFILWLFFTMAVAGIFVLRTRFKHLDRPYKVPLYPLVPLIGIIG
SGYIIISTLITNTFYALCGIGITLLGLPVYSYIKKKNKV

Sequences:

>Translated_439_residues
MKNAKEENLQKNLGLFDAIAIVIGMVIGSGIFFKPAIVFKNAGSPMLGILAWLVGGLITIASGLTIAEIAAAIPKTGGIF
VYIKELYGEKLAFLLGWVQSIVYIPGSAAALSIILSIQITSFIPLTAIEQKVLAIVFIIFIMILNIISTKLVSKMQGIIS
IAKLIPIIAIIALGFIKGTAKGDILNISSSGTVSGFGAALLGTLWAYDGWISVGNMAGELKNPKKDLPRSIIIGLGTTIV
VYVLINLALINIIPMGAIITSEKPASDAAVVLLGSTGAKIISAGILISIFGALNGYLMTGVRIPLAMSQDRSIPFSKYFS
KVHEKSGTPVNAFIFETVLACLYVLSGSFNVLTDLVMFILWLFFTMAVAGIFVLRTRFKHLDRPYKVPLYPLVPLIGIIG
SGYIIISTLITNTFYALCGIGITLLGLPVYSYIKKKNKV
>Mature_439_residues
MKNAKEENLQKNLGLFDAIAIVIGMVIGSGIFFKPAIVFKNAGSPMLGILAWLVGGLITIASGLTIAEIAAAIPKTGGIF
VYIKELYGEKLAFLLGWVQSIVYIPGSAAALSIILSIQITSFIPLTAIEQKVLAIVFIIFIMILNIISTKLVSKMQGIIS
IAKLIPIIAIIALGFIKGTAKGDILNISSSGTVSGFGAALLGTLWAYDGWISVGNMAGELKNPKKDLPRSIIIGLGTTIV
VYVLINLALINIIPMGAIITSEKPASDAAVVLLGSTGAKIISAGILISIFGALNGYLMTGVRIPLAMSQDRSIPFSKYFS
KVHEKSGTPVNAFIFETVLACLYVLSGSFNVLTDLVMFILWLFFTMAVAGIFVLRTRFKHLDRPYKVPLYPLVPLIGIIG
SGYIIISTLITNTFYALCGIGITLLGLPVYSYIKKKNKV

Specific function: Exhibits an obligate exchange activity for serine, threonine and aromatic amino acids [H]

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family [H]

Homologues:

Organism=Homo sapiens, GI71979932, Length=445, Percent_Identity=32.5842696629214, Blast_Score=182, Evalue=5e-46,
Organism=Homo sapiens, GI187423910, Length=426, Percent_Identity=30.9859154929577, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI7657591, Length=426, Percent_Identity=30.9859154929577, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI33286428, Length=454, Percent_Identity=30.6167400881057, Blast_Score=178, Evalue=8e-45,
Organism=Homo sapiens, GI115648063, Length=440, Percent_Identity=27.9545454545455, Blast_Score=175, Evalue=9e-44,
Organism=Homo sapiens, GI115648022, Length=440, Percent_Identity=27.9545454545455, Blast_Score=175, Evalue=9e-44,
Organism=Homo sapiens, GI186910308, Length=437, Percent_Identity=28.1464530892449, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI186910306, Length=437, Percent_Identity=28.1464530892449, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI186910304, Length=437, Percent_Identity=28.1464530892449, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI7657683, Length=437, Percent_Identity=28.8329519450801, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI9790235, Length=437, Percent_Identity=27.9176201372998, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI33286430, Length=250, Percent_Identity=31.6, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI4507047, Length=426, Percent_Identity=23.2394366197183, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI71999149, Length=423, Percent_Identity=25.531914893617, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI258614005, Length=431, Percent_Identity=22.2737819025522, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI258614003, Length=431, Percent_Identity=22.2737819025522, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI258645169, Length=417, Percent_Identity=22.3021582733813, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI114326544, Length=411, Percent_Identity=22.1411192214112, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI114326550, Length=411, Percent_Identity=22.1411192214112, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI110347453, Length=349, Percent_Identity=24.9283667621776, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI87082250, Length=445, Percent_Identity=30.3370786516854, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI2367353, Length=355, Percent_Identity=28.169014084507, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI87081708, Length=434, Percent_Identity=25.3456221198157, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI1788480, Length=434, Percent_Identity=24.8847926267281, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1786694, Length=450, Percent_Identity=24, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1790653, Length=407, Percent_Identity=20.6388206388206, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI87081915, Length=339, Percent_Identity=25.0737463126844, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790575, Length=338, Percent_Identity=22.4852071005917, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1790584, Length=352, Percent_Identity=27.2727272727273, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1786789, Length=424, Percent_Identity=20.9905660377358, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1787890, Length=367, Percent_Identity=20.708446866485, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1786302, Length=446, Percent_Identity=21.9730941704036, Blast_Score=62, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17540018, Length=452, Percent_Identity=31.1946902654867, Blast_Score=168, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17568033, Length=441, Percent_Identity=27.891156462585, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI71993915, Length=405, Percent_Identity=27.4074074074074, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI71995388, Length=458, Percent_Identity=26.6375545851528, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71982612, Length=444, Percent_Identity=26.3513513513513, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI71995382, Length=458, Percent_Identity=26.6375545851528, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI71982608, Length=444, Percent_Identity=26.3513513513513, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI32566699, Length=410, Percent_Identity=27.8048780487805, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI32566695, Length=399, Percent_Identity=26.5664160401003, Blast_Score=117, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI193208714, Length=399, Percent_Identity=26.5664160401003, Blast_Score=117, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI71990446, Length=454, Percent_Identity=25.3303964757709, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71982623, Length=273, Percent_Identity=27.1062271062271, Blast_Score=101, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI71987339, Length=368, Percent_Identity=25.2717391304348, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17531343, Length=405, Percent_Identity=24.1975308641975, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17533459, Length=410, Percent_Identity=24.8780487804878, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17532491, Length=397, Percent_Identity=22.6700251889169, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321492, Length=352, Percent_Identity=23.8636363636364, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6321751, Length=399, Percent_Identity=25.062656641604, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324990, Length=436, Percent_Identity=22.2477064220184, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6320772, Length=440, Percent_Identity=22.5, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24641993, Length=398, Percent_Identity=30.6532663316583, Blast_Score=172, Evalue=5e-43,
Organism=Drosophila melanogaster, GI24641995, Length=398, Percent_Identity=30.6532663316583, Blast_Score=172, Evalue=6e-43,
Organism=Drosophila melanogaster, GI45550968, Length=439, Percent_Identity=28.7015945330296, Blast_Score=171, Evalue=1e-42,
Organism=Drosophila melanogaster, GI19921172, Length=439, Percent_Identity=28.7015945330296, Blast_Score=171, Evalue=1e-42,
Organism=Drosophila melanogaster, GI21357367, Length=435, Percent_Identity=27.816091954023, Blast_Score=161, Evalue=8e-40,
Organism=Drosophila melanogaster, GI24651635, Length=442, Percent_Identity=28.0542986425339, Blast_Score=158, Evalue=7e-39,
Organism=Drosophila melanogaster, GI24651633, Length=442, Percent_Identity=28.0542986425339, Blast_Score=158, Evalue=7e-39,
Organism=Drosophila melanogaster, GI221331183, Length=435, Percent_Identity=28.2758620689655, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI17647653, Length=435, Percent_Identity=28.2758620689655, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24664379, Length=435, Percent_Identity=28.2758620689655, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI221512776, Length=415, Percent_Identity=23.6144578313253, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24666159, Length=415, Percent_Identity=23.6144578313253, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24667468, Length=412, Percent_Identity=25.7281553398058, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24668806, Length=409, Percent_Identity=23.7163814180929, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21356285, Length=409, Percent_Identity=23.7163814180929, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24668802, Length=409, Percent_Identity=23.7163814180929, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281366235, Length=375, Percent_Identity=23.2, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI116007820, Length=375, Percent_Identity=23.2, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 46852; Mature: 46852

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNAKEENLQKNLGLFDAIAIVIGMVIGSGIFFKPAIVFKNAGSPMLGILAWLVGGLITI
CCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
ASGLTIAEIAAAIPKTGGIFVYIKELYGEKLAFLLGWVQSIVYIPGSAAALSIILSIQIT
HCCCHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
SFIPLTAIEQKVLAIVFIIFIMILNIISTKLVSKMQGIISIAKLIPIIAIIALGFIKGTA
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KGDILNISSSGTVSGFGAALLGTLWAYDGWISVGNMAGELKNPKKDLPRSIIIGLGTTIV
CCCEEEECCCCCCHHHHHHHHHHHHHHCCHHHHCCHHHHCCCCHHHCCHHHHHHHHHHHH
VYVLINLALINIIPMGAIITSEKPASDAAVVLLGSTGAKIISAGILISIFGALNGYLMTG
HHHHHHHHHHHHHHCCCEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHC
VRIPLAMSQDRSIPFSKYFSKVHEKSGTPVNAFIFETVLACLYVLSGSFNVLTDLVMFIL
EEECEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
WLFFTMAVAGIFVLRTRFKHLDRPYKVPLYPLVPLIGIIGSGYIIISTLITNTFYALCGI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
GITLLGLPVYSYIKKKNKV
HHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKNAKEENLQKNLGLFDAIAIVIGMVIGSGIFFKPAIVFKNAGSPMLGILAWLVGGLITI
CCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
ASGLTIAEIAAAIPKTGGIFVYIKELYGEKLAFLLGWVQSIVYIPGSAAALSIILSIQIT
HCCCHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
SFIPLTAIEQKVLAIVFIIFIMILNIISTKLVSKMQGIISIAKLIPIIAIIALGFIKGTA
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KGDILNISSSGTVSGFGAALLGTLWAYDGWISVGNMAGELKNPKKDLPRSIIIGLGTTIV
CCCEEEECCCCCCHHHHHHHHHHHHHHCCHHHHCCHHHHCCCCHHHCCHHHHHHHHHHHH
VYVLINLALINIIPMGAIITSEKPASDAAVVLLGSTGAKIISAGILISIFGALNGYLMTG
HHHHHHHHHHHHHHCCCEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHC
VRIPLAMSQDRSIPFSKYFSKVHEKSGTPVNAFIFETVLACLYVLSGSFNVLTDLVMFIL
EEECEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
WLFFTMAVAGIFVLRTRFKHLDRPYKVPLYPLVPLIGIIGSGYIIISTLITNTFYALCGI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
GITLLGLPVYSYIKKKNKV
HHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]