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The map label for this gene is nagB

Identifier: 170756475

GI number: 170756475

Start: 3026539

End: 3027273

Strand: Reverse

Name: nagB

Synonym: CLD_1741

Alternate gene names: 170756475

Gene position: 3027273-3026539 (Counterclockwise)

Preceding gene: 170756790

Following gene: 170754702

Centisome position: 76.48

GC content: 28.03

Gene sequence:

>735_bases
ATGAGAATTATAGTAGTGGATAATTATGAAGAAATGAGTAAAAAAGCAGCAGCTATGATAGCTAGCCAAGTTATTTTAAA
ACCAGATAGTGTCCTTGGATTGGCAACTGGAGATACCCCTATAGGAATGTATAAAGAAATCATAAATATTTATAAAAATG
AAAAGATGAATTTCTCTAAAGTTAAAACTTTTAATTTAGATGAATACTATGGGCTAAATAGAGAAAATCCTCAAAGTTAT
TATTATTACATGATGAATAATTTATTTAATCATGTAAATATTGATAAAAATAATATTAATATTCCTAATGGAATGGCTGA
TAACATAGAAGTAGAGTGTAAGGAATATGAAAGAAAAATAGATAAAGCAGGTGGTATTGATTTACAAATTCTTGGTATTG
GTGTCAATGGCCATATTGGGTTTAATGAACCCAATATAAGTTTTGAATCAGAAACTCATTTAGTTAATCTAAATGAAAAA
ACTATAGAGTCTAATTCAAGATTTTTTAGCTCGAAAGAGGAAGTTCCAACAAAAGCTATTAGTATGGGGATTAAAAGTAT
AATACATTCAAAAAAGATAATCCTATTAGCCTGTGGTAGTGCAAAATCTGATGCTGTTTCTAAAGCTATAAATGGTAAAA
TTAACCCTAATATACCTGCATCTATTCTTCAATTACACAGAGATGTTGTTGTTATTATAGATAAGGAAGCTGCTAGCAAG
TTAAAATTAAAGTAG

Upstream 100 bases:

>100_bases
ATTGTTTATAAAAAATGAAGTATTATGACTTAAATTATTAGACACAATTAGTTATTGATTTAATAAAAGAGAAATTTAAA
TTTTAATGAGGTGTGTTTTT

Downstream 100 bases:

>100_bases
AGTAATTTAAAATATAAGTGTTAGGAATTAATGTCCTTAAGTTATGAGGAAGAATTGCCTAACACTTTTTTATATTCATT
AATATGTAAAATATACAAAA

Product: glucosamine-6-phosphate deaminase

Products: NA

Alternate protein names: GlcN6P deaminase; GNPDA; Glucosamine-6-phosphate isomerase [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MRIIVVDNYEEMSKKAAAMIASQVILKPDSVLGLATGDTPIGMYKEIINIYKNEKMNFSKVKTFNLDEYYGLNRENPQSY
YYYMMNNLFNHVNIDKNNINIPNGMADNIEVECKEYERKIDKAGGIDLQILGIGVNGHIGFNEPNISFESETHLVNLNEK
TIESNSRFFSSKEEVPTKAISMGIKSIIHSKKIILLACGSAKSDAVSKAINGKINPNIPASILQLHRDVVVIIDKEAASK
LKLK

Sequences:

>Translated_244_residues
MRIIVVDNYEEMSKKAAAMIASQVILKPDSVLGLATGDTPIGMYKEIINIYKNEKMNFSKVKTFNLDEYYGLNRENPQSY
YYYMMNNLFNHVNIDKNNINIPNGMADNIEVECKEYERKIDKAGGIDLQILGIGVNGHIGFNEPNISFESETHLVNLNEK
TIESNSRFFSSKEEVPTKAISMGIKSIIHSKKIILLACGSAKSDAVSKAINGKINPNIPASILQLHRDVVVIIDKEAASK
LKLK
>Mature_244_residues
MRIIVVDNYEEMSKKAAAMIASQVILKPDSVLGLATGDTPIGMYKEIINIYKNEKMNFSKVKTFNLDEYYGLNRENPQSY
YYYMMNNLFNHVNIDKNNINIPNGMADNIEVECKEYERKIDKAGGIDLQILGIGVNGHIGFNEPNISFESETHLVNLNEK
TIESNSRFFSSKEEVPTKAISMGIKSIIHSKKIILLACGSAKSDAVSKAINGKINPNIPASILQLHRDVVVIIDKEAASK
LKLK

Specific function: Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion [H]

COG id: COG0363

COG function: function code G; 6-phosphogluconolactonase/Glucosamine-6-phosphate isomerase/deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily [H]

Homologues:

Organism=Homo sapiens, GI19923881, Length=250, Percent_Identity=41.2, Blast_Score=207, Evalue=5e-54,
Organism=Homo sapiens, GI13027378, Length=250, Percent_Identity=40, Blast_Score=199, Evalue=2e-51,
Organism=Escherichia coli, GI1786893, Length=250, Percent_Identity=39.2, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1789530, Length=229, Percent_Identity=27.9475982532751, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI48994958, Length=235, Percent_Identity=27.6595744680851, Blast_Score=102, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17554876, Length=249, Percent_Identity=40.5622489959839, Blast_Score=206, Evalue=7e-54,
Organism=Drosophila melanogaster, GI24581960, Length=250, Percent_Identity=39.2, Blast_Score=204, Evalue=6e-53,
Organism=Drosophila melanogaster, GI19920764, Length=250, Percent_Identity=39.2, Blast_Score=204, Evalue=6e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006148
- InterPro:   IPR004547
- InterPro:   IPR018321 [H]

Pfam domain/function: PF01182 Glucosamine_iso [H]

EC number: =3.5.99.6 [H]

Molecular weight: Translated: 27303; Mature: 27303

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: PS01161 GLC_GALNAC_ISOMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIIVVDNYEEMSKKAAAMIASQVILKPDSVLGLATGDTPIGMYKEIINIYKNEKMNFSK
CEEEEECCHHHHHHHHHHHHHHHHEECCCCEEEEECCCCCHHHHHHHHHHHHCCCCCHHH
VKTFNLDEYYGLNRENPQSYYYYMMNNLFNHVNIDKNNINIPNGMADNIEVECKEYERKI
EEEECCHHHCCCCCCCCCCEEHEEHHHHHHHCCCCCCCCCCCCCCCCCEEEEHHHHHHHH
DKAGGIDLQILGIGVNGHIGFNEPNISFESETHLVNLNEKTIESNSRFFSSKEEVPTKAI
HHCCCCEEEEEEEEECCEECCCCCCCCCCCCEEEEECCHHHHCCCCHHCCCCHHCCHHHH
SMGIKSIIHSKKIILLACGSAKSDAVSKAINGKINPNIPASILQLHRDVVVIIDKEAASK
HHHHHHHHCCCEEEEEEECCCCHHHHHHHHCCCCCCCCCHHHHHHCCCEEEEEECCCCCC
LKLK
CCCC
>Mature Secondary Structure
MRIIVVDNYEEMSKKAAAMIASQVILKPDSVLGLATGDTPIGMYKEIINIYKNEKMNFSK
CEEEEECCHHHHHHHHHHHHHHHHEECCCCEEEEECCCCCHHHHHHHHHHHHCCCCCHHH
VKTFNLDEYYGLNRENPQSYYYYMMNNLFNHVNIDKNNINIPNGMADNIEVECKEYERKI
EEEECCHHHCCCCCCCCCCEEHEEHHHHHHHCCCCCCCCCCCCCCCCCEEEEHHHHHHHH
DKAGGIDLQILGIGVNGHIGFNEPNISFESETHLVNLNEKTIESNSRFFSSKEEVPTKAI
HHCCCCEEEEEEEEECCEECCCCCCCCCCCCEEEEECCHHHHCCCCHHCCCCHHCCHHHH
SMGIKSIIHSKKIILLACGSAKSDAVSKAINGKINPNIPASILQLHRDVVVIIDKEAASK
HHHHHHHHCCCEEEEEEECCCCHHHHHHHHCCCCCCCCCHHHHHHCCCEEEEEECCCCCC
LKLK
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA