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The map label for this gene is rpoA

Identifier: 170756437

GI number: 170756437

Start: 3763409

End: 3764356

Strand: Reverse

Name: rpoA

Synonym: CLD_1053

Alternate gene names: 170756437

Gene position: 3764356-3763409 (Counterclockwise)

Preceding gene: 170755638

Following gene: 170757696

Centisome position: 95.1

GC content: 32.07

Gene sequence:

>948_bases
ATGTTAGAAATAGAAAAGCCAAAAATAGAATGTGTTGAAAATGCTGAAGATGGTTCTTATGGTAAATTTGTCATTGAACC
ATTAGAAAGAGGTTATGGAATAACTCTTGGTAATGCTTTGAGAAGAATTCTTTTATCGTCATTACCTGGAGTTGCTGCAG
ACCATATAAAAATTGACAGTGTTCTTCATGAGTTTTCAACAGTACAAGGTGTAAAAGAGGATGTTACAGAATTAATTCTT
AACATCAAATGTTTAGCACTTACTATGAATGGAGAAGGTCCAAAAACTATATATATAGATGAAGTAGGACCTAAAGAAGT
TACAGCAGCTGATATCAAAACAGACGGAGATGTTGAAGTAATAAACAAAGATTTACATATAGCAACCCTTGACGAAAATG
GTAAGATGTATATGGAAATCAACGTTAATCGTGGTAGGGGCTATGTAACTCAAAACAAGAATAAAACTAAGGATATGCCA
ATAGGAAGTATAGCAGTAGATTCAATTTATACACCTGTTAAAAGAGTTAACTTTTCCGTTGAAAATACTAGAGTTGGTCA
AATAACAGACTATGATAAATTGACTATAGAAGTTTGGACTAATGGAACTATAAGACCAGAAGAAGCTGTAAGTTTATCAG
CTAAAATTCTTATAGAGCACTTTAAGTTATTTATGACTTTAACTGACCATGCAGACGATATGGAAATCATGGTTGAAAAA
GAAGAAGATAAGAAAGAAAAGGTTCTAGAAATGACAATTGAAGAACTAGATCTTTCAGTAAGAAGTTATAACTGCTTAAA
AAGAGCTGGAATAAATACAGTTCAAGAACTATGCGAAAGAAGCATGGATGATATGATGAAAGTTAGAAACCTTGGTAAAA
AGTCCTTAGAAGAAGTAGAACAAAAGTTAGAAGCTTTAGGATTAGGCTTAAGAAAAAGTGAAGATTAG

Upstream 100 bases:

>100_bases
GAAACATTAATCGTTGAGTTATACAGCAAATAATAAATAATTTAATTTAGATCAGTTGCCCTCAAAAAGTATAAATATTA
ATAAATGAGGAGGGTTAAGA

Downstream 100 bases:

>100_bases
TAAAGTTAATATAGGCAGTTTAAGGTAAGGAGGTATAGGAGAGATGGCAGGATATCGTAAATTAGGTCGTCCAACTGATC
AACGTAAAGCAATGCTTAGA

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MLEIEKPKIECVENAEDGSYGKFVIEPLERGYGITLGNALRRILLSSLPGVAADHIKIDSVLHEFSTVQGVKEDVTELIL
NIKCLALTMNGEGPKTIYIDEVGPKEVTAADIKTDGDVEVINKDLHIATLDENGKMYMEINVNRGRGYVTQNKNKTKDMP
IGSIAVDSIYTPVKRVNFSVENTRVGQITDYDKLTIEVWTNGTIRPEEAVSLSAKILIEHFKLFMTLTDHADDMEIMVEK
EEDKKEKVLEMTIEELDLSVRSYNCLKRAGINTVQELCERSMDDMMKVRNLGKKSLEEVEQKLEALGLGLRKSED

Sequences:

>Translated_315_residues
MLEIEKPKIECVENAEDGSYGKFVIEPLERGYGITLGNALRRILLSSLPGVAADHIKIDSVLHEFSTVQGVKEDVTELIL
NIKCLALTMNGEGPKTIYIDEVGPKEVTAADIKTDGDVEVINKDLHIATLDENGKMYMEINVNRGRGYVTQNKNKTKDMP
IGSIAVDSIYTPVKRVNFSVENTRVGQITDYDKLTIEVWTNGTIRPEEAVSLSAKILIEHFKLFMTLTDHADDMEIMVEK
EEDKKEKVLEMTIEELDLSVRSYNCLKRAGINTVQELCERSMDDMMKVRNLGKKSLEEVEQKLEALGLGLRKSED
>Mature_315_residues
MLEIEKPKIECVENAEDGSYGKFVIEPLERGYGITLGNALRRILLSSLPGVAADHIKIDSVLHEFSTVQGVKEDVTELIL
NIKCLALTMNGEGPKTIYIDEVGPKEVTAADIKTDGDVEVINKDLHIATLDENGKMYMEINVNRGRGYVTQNKNKTKDMP
IGSIAVDSIYTPVKRVNFSVENTRVGQITDYDKLTIEVWTNGTIRPEEAVSLSAKILIEHFKLFMTLTDHADDMEIMVEK
EEDKKEKVLEMTIEELDLSVRSYNCLKRAGINTVQELCERSMDDMMKVRNLGKKSLEEVEQKLEALGLGLRKSED

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=317, Percent_Identity=44.794952681388, Blast_Score=249, Evalue=2e-67,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_CLOB1 (A7FZ44)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001385767.1
- ProteinModelPortal:   A7FZ44
- STRING:   A7FZ44
- GeneID:   5397605
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_3508
- eggNOG:   COG0202
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- ProtClustDB:   PRK05182
- BioCyc:   CBOT441770:CLB_3508-MONOMER
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 35397; Mature: 35397

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEIEKPKIECVENAEDGSYGKFVIEPLERGYGITLGNALRRILLSSLPGVAADHIKIDS
CCCCCCCCHHEECCCCCCCCCCEEEEHHHCCCCEEHHHHHHHHHHHHCCCCCHHHHHHHH
VLHEFSTVQGVKEDVTELILNIKCLALTMNGEGPKTIYIDEVGPKEVTAADIKTDGDVEV
HHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCEEEEECCCCCCEEEECCCCCCCEEE
INKDLHIATLDENGKMYMEINVNRGRGYVTQNKNKTKDMPIGSIAVDSIYTPVKRVNFSV
EECCEEEEEECCCCEEEEEEEEECCCEEEECCCCCCCCCCCCCEEHHHHHCCHHHHEEEE
ENTRVGQITDYDKLTIEVWTNGTIRPEEAVSLSAKILIEHFKLFMTLTDHADDMEIMVEK
CCCEECCEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEEEC
EEDKKEKVLEMTIEELDLSVRSYNCLKRAGINTVQELCERSMDDMMKVRNLGKKSLEEVE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
QKLEALGLGLRKSED
HHHHHHCCCCCCCCC
>Mature Secondary Structure
MLEIEKPKIECVENAEDGSYGKFVIEPLERGYGITLGNALRRILLSSLPGVAADHIKIDS
CCCCCCCCHHEECCCCCCCCCCEEEEHHHCCCCEEHHHHHHHHHHHHCCCCCHHHHHHHH
VLHEFSTVQGVKEDVTELILNIKCLALTMNGEGPKTIYIDEVGPKEVTAADIKTDGDVEV
HHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCEEEEECCCCCCEEEECCCCCCCEEE
INKDLHIATLDENGKMYMEINVNRGRGYVTQNKNKTKDMPIGSIAVDSIYTPVKRVNFSV
EECCEEEEEECCCCEEEEEEEEECCCEEEECCCCCCCCCCCCCEEHHHHHCCHHHHEEEE
ENTRVGQITDYDKLTIEVWTNGTIRPEEAVSLSAKILIEHFKLFMTLTDHADDMEIMVEK
CCCEECCEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEEEC
EEDKKEKVLEMTIEELDLSVRSYNCLKRAGINTVQELCERSMDDMMKVRNLGKKSLEEVE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
QKLEALGLGLRKSED
HHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA