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The map label for this gene is ackA

Identifier: 170756436

GI number: 170756436

Start: 2579408

End: 2580601

Strand: Reverse

Name: ackA

Synonym: CLD_2181

Alternate gene names: 170756436

Gene position: 2580601-2579408 (Counterclockwise)

Preceding gene: 170757396

Following gene: 170754849

Centisome position: 65.2

GC content: 32.91

Gene sequence:

>1194_bases
ATGAAAATATTAGTAGTTAACTGCGGAAGTTCGTCATTAAAGTATCAATTAATAGATATTACTTCTGAAGAGGCTTTAGC
AAAGGGATTAGTAGAGAGAATAGGAATAGAAGGCTCTATATTGACTCAAAAGGTTAATGGAGAAAAATATATAATAGAAG
AACCTATGAAAGATCATAAGAAAGCTATAGAGTTAGTTTTAAAAGCATTAGTAGACAAAGAGCATGGTGTAATATCTGAT
ATGTCAGAAATAGCAGCAGTAGGACATAGAGTAGTTCACGGTGGAGAAAAATATGCTAGCTCAGTACTTATAGATGATGA
GGTTATGAAGGCTTTAGAAGATTGTGTTAAATTAGCACCACTTCATAATCCACCAAACATAATAGGAATAAATGCTTGTA
GAGAACTTATGCCTAAAACTCCAATGGTAGCAGTATTTGATACAGCATTCCATCAAACATTACCAGACTATGCATATATG
TATCCATTGCCATATGAATTATATGAACAAAATGGTATAAGAAAATATGGTTTCCATGGAACATCACATAGATATGTATC
CAGTGTAGCGTCAGAGATGATGGAAAAAGATCTTAAAGATCTTAAAGTAATAACTTGTCACTTAGGAAATGGAGCAAGTT
TATGTGCTGTAAAAGAAGGAAAATCAGTAGAAACCTCTATGGGATTCACTCCACTTGCAGGATTAGCTATGGGAACTAGA
TGCGGAGATATAGATCCAGCAATACTTCTTTTCATGGAAAGAGAATTAAAGATGTCACCAGATGAAGTAGATACTGTTAT
AAATAAAAAATCAGGTGTATTAGGAATATCAGGAGTAAGTAGTGACTTTAGAGATATAGAAGGTGCCGCAGAAGAAGGAA
ATAAAAGAGCTAAACTAGCTTTAGATGTATATCATTACACAGTAAGACAGACAATCGGTGCTTATACAGCAGTATTAAAT
GGTGTAGATGCTATAGTATTTACAGCAGGACTAGGAGAAAATTCAGCAGCAAGCAGAGAAGAAATTTTAAATGGATTAGA
ATACTTAGGTATTAAGATAGATGCTGAAAAGAATAAGCAAAGAGGAAAACAAATAGAAATAAGTACTGAAGATTCAAAGG
TAAAGGTATTCGTTATACCAACTGATGAAGAATTAATGATAGCAAGAGATACTAAAGAAATAACAGTTAAATAA

Upstream 100 bases:

>100_bases
TTTTAGATGATAAATTAATGTATTAATATCACGAATAAAAAAATAGATTTGCTTATAATAAAATTTTATATAAAACATAT
AGATGAAGGGTGGAAAATTA

Downstream 100 bases:

>100_bases
TATGTAATATAAGTTATAAATATAGGTATAATAACTTTGATTTACTTATAAAATACTTGACTTTTTAATATGCCCTTTAT
ATAATAAATTGTGGTTGCTA

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase [H]

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MKILVVNCGSSSLKYQLIDITSEEALAKGLVERIGIEGSILTQKVNGEKYIIEEPMKDHKKAIELVLKALVDKEHGVISD
MSEIAAVGHRVVHGGEKYASSVLIDDEVMKALEDCVKLAPLHNPPNIIGINACRELMPKTPMVAVFDTAFHQTLPDYAYM
YPLPYELYEQNGIRKYGFHGTSHRYVSSVASEMMEKDLKDLKVITCHLGNGASLCAVKEGKSVETSMGFTPLAGLAMGTR
CGDIDPAILLFMERELKMSPDEVDTVINKKSGVLGISGVSSDFRDIEGAAEEGNKRAKLALDVYHYTVRQTIGAYTAVLN
GVDAIVFTAGLGENSAASREEILNGLEYLGIKIDAEKNKQRGKQIEISTEDSKVKVFVIPTDEELMIARDTKEITVK

Sequences:

>Translated_397_residues
MKILVVNCGSSSLKYQLIDITSEEALAKGLVERIGIEGSILTQKVNGEKYIIEEPMKDHKKAIELVLKALVDKEHGVISD
MSEIAAVGHRVVHGGEKYASSVLIDDEVMKALEDCVKLAPLHNPPNIIGINACRELMPKTPMVAVFDTAFHQTLPDYAYM
YPLPYELYEQNGIRKYGFHGTSHRYVSSVASEMMEKDLKDLKVITCHLGNGASLCAVKEGKSVETSMGFTPLAGLAMGTR
CGDIDPAILLFMERELKMSPDEVDTVINKKSGVLGISGVSSDFRDIEGAAEEGNKRAKLALDVYHYTVRQTIGAYTAVLN
GVDAIVFTAGLGENSAASREEILNGLEYLGIKIDAEKNKQRGKQIEISTEDSKVKVFVIPTDEELMIARDTKEITVK
>Mature_397_residues
MKILVVNCGSSSLKYQLIDITSEEALAKGLVERIGIEGSILTQKVNGEKYIIEEPMKDHKKAIELVLKALVDKEHGVISD
MSEIAAVGHRVVHGGEKYASSVLIDDEVMKALEDCVKLAPLHNPPNIIGINACRELMPKTPMVAVFDTAFHQTLPDYAYM
YPLPYELYEQNGIRKYGFHGTSHRYVSSVASEMMEKDLKDLKVITCHLGNGASLCAVKEGKSVETSMGFTPLAGLAMGTR
CGDIDPAILLFMERELKMSPDEVDTVINKKSGVLGISGVSSDFRDIEGAAEEGNKRAKLALDVYHYTVRQTIGAYTAVLN
GVDAIVFTAGLGENSAASREEILNGLEYLGIKIDAEKNKQRGKQIEISTEDSKVKVFVIPTDEELMIARDTKEITVK

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=403, Percent_Identity=45.409429280397, Blast_Score=343, Evalue=1e-95,
Organism=Escherichia coli, GI145693184, Length=393, Percent_Identity=43.2569974554707, Blast_Score=323, Evalue=9e-90,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 43486; Mature: 43486

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVVNCGSSSLKYQLIDITSEEALAKGLVERIGIEGSILTQKVNGEKYIIEEPMKDHK
CEEEEEECCCCCEEEEEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCEEEECCCHHHHH
KAIELVLKALVDKEHGVISDMSEIAAVGHRVVHGGEKYASSVLIDDEVMKALEDCVKLAP
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHEEECHHHHHHHHHHHHHCC
LHNPPNIIGINACRELMPKTPMVAVFDTAFHQTLPDYAYMYPLPYELYEQNGIRKYGFHG
CCCCCCEEEHHHHHHHCCCCCEEEEEHHHHHHHCCCCEEECCCCHHHHHCCCCEEECCCC
TSHRYVSSVASEMMEKDLKDLKVITCHLGNGASLCAVKEGKSVETSMGFTPLAGLAMGTR
CCHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCEEEECCCCCCCCCCCCCHHHHHHHCCC
CGDIDPAILLFMERELKMSPDEVDTVINKKSGVLGISGVSSDFRDIEGAAEEGNKRAKLA
CCCCCHHHHHEEHHHCCCCHHHHHHHHCCCCCEEEECCCCCHHHHHCCHHHCCCCEEEEE
LDVYHYTVRQTIGAYTAVLNGVDAIVFTAGLGENSAASREEILNGLEYLGIKIDAEKNKQ
EHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHCHHHHEEEEEECCCCCC
RGKQIEISTEDSKVKVFVIPTDEELMIARDTKEITVK
CCCEEEEECCCCEEEEEEEECCCCEEEEECCCEEEEC
>Mature Secondary Structure
MKILVVNCGSSSLKYQLIDITSEEALAKGLVERIGIEGSILTQKVNGEKYIIEEPMKDHK
CEEEEEECCCCCEEEEEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCEEEECCCHHHHH
KAIELVLKALVDKEHGVISDMSEIAAVGHRVVHGGEKYASSVLIDDEVMKALEDCVKLAP
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHEEECHHHHHHHHHHHHHCC
LHNPPNIIGINACRELMPKTPMVAVFDTAFHQTLPDYAYMYPLPYELYEQNGIRKYGFHG
CCCCCCEEEHHHHHHHCCCCCEEEEEHHHHHHHCCCCEEECCCCHHHHHCCCCEEECCCC
TSHRYVSSVASEMMEKDLKDLKVITCHLGNGASLCAVKEGKSVETSMGFTPLAGLAMGTR
CCHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCEEEECCCCCCCCCCCCCHHHHHHHCCC
CGDIDPAILLFMERELKMSPDEVDTVINKKSGVLGISGVSSDFRDIEGAAEEGNKRAKLA
CCCCCHHHHHEEHHHCCCCHHHHHHHHCCCCCEEEECCCCCHHHHHCCHHHCCCCEEEEE
LDVYHYTVRQTIGAYTAVLNGVDAIVFTAGLGENSAASREEILNGLEYLGIKIDAEKNKQ
EHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHCHHHHEEEEEECCCCCC
RGKQIEISTEDSKVKVFVIPTDEELMIARDTKEITVK
CCCEEEEECCCCEEEEEEEECCCCEEEEECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA