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The map label for this gene is vanS [H]

Identifier: 170756435

GI number: 170756435

Start: 321191

End: 322309

Strand: Direct

Name: vanS [H]

Synonym: CLD_0505

Alternate gene names: 170756435

Gene position: 321191-322309 (Clockwise)

Preceding gene: 170755164

Following gene: 170757812

Centisome position: 8.11

GC content: 29.4

Gene sequence:

>1119_bases
TTGAAGCGTAAAAAATGGAAATGGAATCATTATATTATAGCATTTATAGCATTTATCGTCATATTAATTTTTATTGATGT
GTATTTAGAAGGAAGTGTTGGTGAATACTTTGTAAGGACATTACTTGATGATAGAGAAGAAGTAATATTAAGTTACAGTG
AACATCATGCAATAAGGGGAACTTTTAGATGGGATAGATTAAGAAGCATATTAACATTTATAGCTGTCACAGGTTTCTTA
ATAGTAGAAATTAGTATTTATTTTGCTTCTAAAATATCGCGAAATCGTGAGAAGGAGAAAGTAGTAACCCAAGTGGAGGA
GAGACTATGTCAGTTTAGAGATGATAAAAATCCTACTGAAGTTCAAGAGTTAAGGCCTATTGATGTGGGAATTAAATCAA
TTTTAAATGAAAAGGCAAGAATAGAACAAGAAAAAGAAATGGAAATTCAAAAGAAAAATGATTTGGTAACTTATTTGGCA
CATGATTTAAAAACACCATTAACAGCTATTATTGGTTACTTGAGTATTTTGGAAGAATCTGAAGTTCCAGAAAAAATTCA
GAAAGATTATTTAAAGAAAGTTTTAGATAAAGCCTATCATTTAGAGGAATTAACCAATCAGTTTTTTGATATTACAAGGT
TTAATCTTCAAGAAATTCCAATAAATAAAACAAAAATTGATGGAGAATTTTTTTTGCAACAGATAACAGATGAGTTTTAT
CCATTGTGTTTACCAAAAAAACTACAGATTGATTTAAATATATCACCAAACTTTAAAATATATGGTGATGGTGGTCTTTT
GGCAAGAGTTTTAAATAATATTCTAAAAAATGCTATCAGCTATAGTAATAAGAATTCTGTTATTAAAGTTACAGGTAAAG
AAGAGGGAGAAATAACTAGTATAACTGCGGAAAATGATGGAGATGTTATATCAAATCAAGAACTTGAACTAATATTTCAA
AAATTCTACCGCAGAGATAAAGCAAGAAGTCAATCATCAGGGTCAGGACTGGGACTTGCTATTGCTAAAGAAATAGTAAT
GCGTCATGGCGGAACATTAAAGGCAGAAAGCGTAAATGGTCATACAACCTTTAGTATTCATTTACCAAAATCTTTATAA

Upstream 100 bases:

>100_bases
AATACTATTACTGTTCATGTTAGAAATTTGAGAGAAAAAATGGATGATTCTTTTGAGAAACCAGAGTATATTAAAACAGT
GTGGGGGGTAGGTTATATTA

Downstream 100 bases:

>100_bases
AATCTTTATAAAACATATATAAATTATGAATAAACTTTTGTCTAATAATTGATAAAGTAATGATTAGACAAAAGTTTATT
ATTTTTTGGAGGGTCTAAAA

Product: sensor protein VanS (vancomycin resistance protein vanS)(vancomycin histidine protein kinase)

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MKRKKWKWNHYIIAFIAFIVILIFIDVYLEGSVGEYFVRTLLDDREEVILSYSEHHAIRGTFRWDRLRSILTFIAVTGFL
IVEISIYFASKISRNREKEKVVTQVEERLCQFRDDKNPTEVQELRPIDVGIKSILNEKARIEQEKEMEIQKKNDLVTYLA
HDLKTPLTAIIGYLSILEESEVPEKIQKDYLKKVLDKAYHLEELTNQFFDITRFNLQEIPINKTKIDGEFFLQQITDEFY
PLCLPKKLQIDLNISPNFKIYGDGGLLARVLNNILKNAISYSNKNSVIKVTGKEEGEITSITAENDGDVISNQELELIFQ
KFYRRDKARSQSSGSGLGLAIAKEIVMRHGGTLKAESVNGHTTFSIHLPKSL

Sequences:

>Translated_372_residues
MKRKKWKWNHYIIAFIAFIVILIFIDVYLEGSVGEYFVRTLLDDREEVILSYSEHHAIRGTFRWDRLRSILTFIAVTGFL
IVEISIYFASKISRNREKEKVVTQVEERLCQFRDDKNPTEVQELRPIDVGIKSILNEKARIEQEKEMEIQKKNDLVTYLA
HDLKTPLTAIIGYLSILEESEVPEKIQKDYLKKVLDKAYHLEELTNQFFDITRFNLQEIPINKTKIDGEFFLQQITDEFY
PLCLPKKLQIDLNISPNFKIYGDGGLLARVLNNILKNAISYSNKNSVIKVTGKEEGEITSITAENDGDVISNQELELIFQ
KFYRRDKARSQSSGSGLGLAIAKEIVMRHGGTLKAESVNGHTTFSIHLPKSL
>Mature_372_residues
MKRKKWKWNHYIIAFIAFIVILIFIDVYLEGSVGEYFVRTLLDDREEVILSYSEHHAIRGTFRWDRLRSILTFIAVTGFL
IVEISIYFASKISRNREKEKVVTQVEERLCQFRDDKNPTEVQELRPIDVGIKSILNEKARIEQEKEMEIQKKNDLVTYLA
HDLKTPLTAIIGYLSILEESEVPEKIQKDYLKKVLDKAYHLEELTNQFFDITRFNLQEIPINKTKIDGEFFLQQITDEFY
PLCLPKKLQIDLNISPNFKIYGDGGLLARVLNNILKNAISYSNKNSVIKVTGKEEGEITSITAENDGDVISNQELELIFQ
KFYRRDKARSQSSGSGLGLAIAKEIVMRHGGTLKAESVNGHTTFSIHLPKSL

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786600, Length=252, Percent_Identity=27.3809523809524, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1786912, Length=246, Percent_Identity=30.8943089430894, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI48994928, Length=250, Percent_Identity=24.8, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1790346, Length=252, Percent_Identity=28.5714285714286, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI145693157, Length=248, Percent_Identity=23.7903225806452, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788393, Length=222, Percent_Identity=22.972972972973, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786783, Length=220, Percent_Identity=25.9090909090909, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1788279, Length=313, Percent_Identity=25.8785942492013, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790861, Length=275, Percent_Identity=26.1818181818182, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790436, Length=244, Percent_Identity=28.2786885245902, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788713, Length=241, Percent_Identity=24.896265560166, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788549, Length=244, Percent_Identity=27.4590163934426, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787894, Length=223, Percent_Identity=26.9058295964126, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI87081816, Length=287, Percent_Identity=25.4355400696864, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1789808, Length=228, Percent_Identity=30.2631578947368, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI87082128, Length=238, Percent_Identity=26.4705882352941, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1789149, Length=325, Percent_Identity=24, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787374, Length=295, Percent_Identity=24.0677966101695, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790300, Length=258, Percent_Identity=22.8682170542636, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 42967; Mature: 42967

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKKWKWNHYIIAFIAFIVILIFIDVYLEGSVGEYFVRTLLDDREEVILSYSEHHAIRG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHCCCEEC
TFRWDRLRSILTFIAVTGFLIVEISIYFASKISRNREKEKVVTQVEERLCQFRDDKNPTE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCH
VQELRPIDVGIKSILNEKARIEQEKEMEIQKKNDLVTYLAHDLKTPLTAIIGYLSILEES
HHHHCCHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHC
EVPEKIQKDYLKKVLDKAYHLEELTNQFFDITRFNLQEIPINKTKIDGEFFLQQITDEFY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCEECCHHHHHHHHHHHHC
PLCLPKKLQIDLNISPNFKIYGDGGLLARVLNNILKNAISYSNKNSVIKVTGKEEGEITS
CCCCCCEEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEE
ITAENDGDVISNQELELIFQKFYRRDKARSQSSGSGLGLAIAKEIVMRHGGTLKAESVNG
EEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCC
HTTFSIHLPKSL
EEEEEEECCCCC
>Mature Secondary Structure
MKRKKWKWNHYIIAFIAFIVILIFIDVYLEGSVGEYFVRTLLDDREEVILSYSEHHAIRG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHCCCEEC
TFRWDRLRSILTFIAVTGFLIVEISIYFASKISRNREKEKVVTQVEERLCQFRDDKNPTE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCH
VQELRPIDVGIKSILNEKARIEQEKEMEIQKKNDLVTYLAHDLKTPLTAIIGYLSILEES
HHHHCCHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHC
EVPEKIQKDYLKKVLDKAYHLEELTNQFFDITRFNLQEIPINKTKIDGEFFLQQITDEFY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCEECCHHHHHHHHHHHHC
PLCLPKKLQIDLNISPNFKIYGDGGLLARVLNNILKNAISYSNKNSVIKVTGKEEGEITS
CCCCCCEEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEE
ITAENDGDVISNQELELIFQKFYRRDKARSQSSGSGLGLAIAKEIVMRHGGTLKAESVNG
EEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCC
HTTFSIHLPKSL
EEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]