Click here to switch to the map view.

The map label for this gene is thiM

Identifier: 170756389

GI number: 170756389

Start: 2449433

End: 2450230

Strand: Reverse

Name: thiM

Synonym: CLD_2322

Alternate gene names: 170756389

Gene position: 2450230-2449433 (Counterclockwise)

Preceding gene: 170757388

Following gene: 170754785

Centisome position: 61.9

GC content: 31.83

Gene sequence:

>798_bases
TTGCAAATAAGACAGAGTGTTAAATTAAAAAAACCTCTCATTCACTATATTACAAATCCAATTTCAATAAATGATTGTGC
AAATATAATTCTTGCTGCTGGCGCAAAGCCCATTATGGCCGAGCATCCTTTGGAAGTTTCCGAAATCACTTCTGTTTCAA
AATCCCTTGGAGTTAATCTTGGGAATATAACAGATAATAAAATGAAATCTATGTTGATTTCAGGTAAAACAGCCTATGAA
AATAAGATTCCGCAAGTAATTGATCTTGTAGGCGTAGGTTGCAGTAAACTTCGTCTAGATTATGCAAAGAAATTTATTTT
AGAGTGTCATCCAAATGTTATTAAAGGTAATATGTCCGAAATAAAAGCAATCTATGGCATAAAAAGTAGTGCAAAAGGAA
TTGATGTTGGAGCATGTGATATTATAACAGAGCAAAATTTTGATGAAAATATAGAAATGATAAAGAGGTTATCCATGGAA
ACCGGTTCTGTTGTTGCAGCTACAGGGGTAGTGGATATAATAAGCAATGGTACTTATACATATATAATATCAAATGGTTG
TGAAATGCTTTCAATGATAACTGGAACAGGTTGTATGCTTACTGGAATTATAGCAAGTTATATTTCTTCTGGGAATATAC
TAGAAGGTACTGCTCTTGCTATAGTTCTTATGGGAATATGTGGTGAACTTTCTCAGCATGTTAAGGGCACTGGGAGTTTC
AGAAATGAGCTTATAGATAATATTTTTAGTATTTCCGATGATATTATAATAAAAAAAATAAGAATAAATAGTTATTAA

Upstream 100 bases:

>100_bases
TTTCTAGGAGCATTAAGTAGGACAGGTAATCTTACAAAAATTCAAAGAAGTTTGGGGGCAAAAATTTATGACAAAGCAAG
AATTAATAATAAACAAACTA

Downstream 100 bases:

>100_bases
TTATGGATACTGGATTTTTATTTTACTATATTTCCAATCTGAATATAGTTAATAGCTAATTTTTGTATAAGTGAAACTGT
AAGAAGCTTTAAATAGTTTA

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase 1; TH kinase 1; Thz kinase 1 [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MQIRQSVKLKKPLIHYITNPISINDCANIILAAGAKPIMAEHPLEVSEITSVSKSLGVNLGNITDNKMKSMLISGKTAYE
NKIPQVIDLVGVGCSKLRLDYAKKFILECHPNVIKGNMSEIKAIYGIKSSAKGIDVGACDIITEQNFDENIEMIKRLSME
TGSVVAATGVVDIISNGTYTYIISNGCEMLSMITGTGCMLTGIIASYISSGNILEGTALAIVLMGICGELSQHVKGTGSF
RNELIDNIFSISDDIIIKKIRINSY

Sequences:

>Translated_265_residues
MQIRQSVKLKKPLIHYITNPISINDCANIILAAGAKPIMAEHPLEVSEITSVSKSLGVNLGNITDNKMKSMLISGKTAYE
NKIPQVIDLVGVGCSKLRLDYAKKFILECHPNVIKGNMSEIKAIYGIKSSAKGIDVGACDIITEQNFDENIEMIKRLSME
TGSVVAATGVVDIISNGTYTYIISNGCEMLSMITGTGCMLTGIIASYISSGNILEGTALAIVLMGICGELSQHVKGTGSF
RNELIDNIFSISDDIIIKKIRINSY
>Mature_265_residues
MQIRQSVKLKKPLIHYITNPISINDCANIILAAGAKPIMAEHPLEVSEITSVSKSLGVNLGNITDNKMKSMLISGKTAYE
NKIPQVIDLVGVGCSKLRLDYAKKFILECHPNVIKGNMSEIKAIYGIKSSAKGIDVGACDIITEQNFDENIEMIKRLSME
TGSVVAATGVVDIISNGTYTYIISNGCEMLSMITGTGCMLTGIIASYISSGNILEGTALAIVLMGICGELSQHVKGTGSF
RNELIDNIFSISDDIIIKKIRINSY

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family [H]

Homologues:

Organism=Escherichia coli, GI1788421, Length=251, Percent_Identity=29.8804780876494, Blast_Score=121, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6325042, Length=269, Percent_Identity=29.7397769516729, Blast_Score=92, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000417 [H]

Pfam domain/function: PF02110 HK [H]

EC number: =2.7.1.50 [H]

Molecular weight: Translated: 28568; Mature: 28568

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIRQSVKLKKPLIHYITNPISINDCANIILAAGAKPIMAEHPLEVSEITSVSKSLGVNL
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCC
GNITDNKMKSMLISGKTAYENKIPQVIDLVGVGCSKLRLDYAKKFILECHPNVIKGNMSE
CCCCHHHHHHHHHCCCHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHCCCHHH
IKAIYGIKSSAKGIDVGACDIITEQNFDENIEMIKRLSMETGSVVAATGVVDIISNGTYT
HHHHHCCCCCCCCCCCCHHHEEECCCCCHHHHHHHHHCCCCCCEEEHHHHHHHHCCCEEE
YIISNGCEMLSMITGTGCMLTGIIASYISSGNILEGTALAIVLMGICGELSQHVKGTGSF
EEECCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHH
RNELIDNIFSISDDIIIKKIRINSY
HHHHHHHHHHCCCCCEEEEEEECCC
>Mature Secondary Structure
MQIRQSVKLKKPLIHYITNPISINDCANIILAAGAKPIMAEHPLEVSEITSVSKSLGVNL
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCC
GNITDNKMKSMLISGKTAYENKIPQVIDLVGVGCSKLRLDYAKKFILECHPNVIKGNMSE
CCCCHHHHHHHHHCCCHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHCCCHHH
IKAIYGIKSSAKGIDVGACDIITEQNFDENIEMIKRLSMETGSVVAATGVVDIISNGTYT
HHHHHCCCCCCCCCCCCHHHEEECCCCCHHHHHHHHHCCCCCCEEEHHHHHHHHCCCEEE
YIISNGCEMLSMITGTGCMLTGIIASYISSGNILEGTALAIVLMGICGELSQHVKGTGSF
EEECCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHH
RNELIDNIFSISDDIIIKKIRINSY
HHHHHHHHHHCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA