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The map label for this gene is gyrB

Identifier: 170756292

GI number: 170756292

Start: 4431

End: 6344

Strand: Direct

Name: gyrB

Synonym: CLD_0820

Alternate gene names: 170756292

Gene position: 4431-6344 (Clockwise)

Preceding gene: 170755684

Following gene: 170756835

Centisome position: 0.11

GC content: 31.87

Gene sequence:

>1914_bases
ATGTCACAAGAAAATAAACAAGTTTATGATGAAAGTCAAATTCAAGTGTTGGAAGGTTTAGAAGCTGTTAGAAAAAGACC
AGGAATGTATATTGGAAGCACTAGTTTAAGGGGACTTCATCATTTGGTTTATGAAATAGTTGATAATAGTATAGATGAAG
CGCTAGCTGGTTTTTGTACACATATAGAAGTTTTTATACACAAAGATAATTCTATAACTGTGGTAGACGATGGACGTGGA
ATGCCAGTTGGTATGCATTCAAAGGTAAAAAAACCTGCTGTTGAAGTAATAATGACTATATTACATGCAGGTGGAAAATT
TGGTGGTGGAGGCTACAAGGTTTCTGGTGGACTTCATGGTGTTGGAGCTTCTGTTGTTAATGCCTTATCAGAACAATGTG
AAGTAGAAGTAAGAAGAGAAGGCCATATTTGGAAACAAAAATTCGAAAGAGGTATAACAAAAACTGGATTAGATATAGTT
GGAGATACGGAAGATCATGGTACAAAAATATATTTTAAGCCAGATATTGAAATATTTGATGAATTAGAATTTGAATATGA
TACTTTAGCTCACAGACTCAGAGAATTAGCCTTTTTAAACAAAGGTATAAAAATTTCATTAACAGATGAAAGATATGATA
AAAAAGAAATGTTCCACTATGAGGGCGGATTGAGATCTTTTGTACTTTATTTAAATAGAAATAAGGAAAAATTACATCAA
CAACCTATATATGTTGATGAAAATAAAGATGGATGTATAGTTGAGATTGCTATGCAATATAACGATGGTTATGCAGAAAA
TATTTTTTCCTTTGCTAACAATATAGATACTATAGAAGGTGGAACTCATTTAGCTGGATTTAAATCAGCTCTAACAAGAG
TTATAAATGACTATGCTAGGAAATTTAATTACTTAAAGGATATAGATAAAAATTTATCAGGAGACGATGTTAGGGAAGGT
CTAACAGCAGTTATTTCAGTAAAATTAACAGATCCTCAATTTGAAGGACAGACAAAGACAAAATTAGGAAATGGTGAAGT
AAGAGGAATTGTAGATACAATAGTAGGCCAGAGTATAGGTTCTTTTTTAGAGGAAAATCCTAATGTAGGTAAAATAATAA
TTGAAAAAGGCCTATCAGCTTCAAGAGCTAGAGAGGCTGCAAAAAGAGCAAGAGAACTTACTAGAAGAAAATCTATTTTA
GAAAGCACGTCTCTTCCAGGAAAATTATCAGATTGTTCTTCAAAGGATCCATCATTATGTGAAATTTATTTGGTCGAAGG
AGATTCCGCAGGTGGATCTGCAAAACAAGGAAGAAATAGAGAATTCCAAGCTATATTACCTTTAAAGGGTAAAATAATGA
ATGTGGAAAAGCAAAGATTGGATAAAATATTAGCTTCAGATGAAATACGTGCTATGATAACTGCATTTGGAGCAGGTATA
GGAAAAGAATTTGATATTGATAAAATAAGATATAATAGAATAATTATAATGACAGATGCTGACGTAGATGGAGCCCATAT
AAGAACTTTACTTTTAACATTCTTTTATAGATACATGAAAGAATTAATAGAAGAAGGTCATGTTTATATAGCTCAACCAC
CACTTTATAGAATATATAAGGCTAAAAAAGAAACTTATGTTTATTCAGATCCAGAATTGGATGCTGCATTATTAGAATTG
GGTGGAAAAGATACCAATACTAATATTCAAAGATACAAAGGTCTTGGAGAAATGAATCCAGAACAACTTTGGGAAACTAC
TATGGACCCAGAACATAGAACTTTATTACAGGTAACAGTAGAGGACGCTATGGAGGCAGATGAAATATTTACTATACTTA
TGGGAACAAAAGTAGAACCAAGAAGAGACTTTATAGAAAGTAATGCTGATAAAGTAGTTAACTTGGATATATAA

Upstream 100 bases:

>100_bases
ATAAAAAGAGTAAGATATATTTGTCTCCTATATCTTCAGCTACACTGACTAAAAGAACAAAAGTTTTGTATAATGAGCTG
TAAAAGTTAGGAGGATAAAC

Downstream 100 bases:

>100_bases
GATAAGATTGTAAAGAGGTGTAATCATGCTAAACGAAGGAAAGATTTTGCCTGTAGATGTAAGTAAAGAAATGAAGAAAT
GTTATATAGACTATGCCATG

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 637; Mature: 636

Protein sequence:

>637_residues
MSQENKQVYDESQIQVLEGLEAVRKRPGMYIGSTSLRGLHHLVYEIVDNSIDEALAGFCTHIEVFIHKDNSITVVDDGRG
MPVGMHSKVKKPAVEVIMTILHAGGKFGGGGYKVSGGLHGVGASVVNALSEQCEVEVRREGHIWKQKFERGITKTGLDIV
GDTEDHGTKIYFKPDIEIFDELEFEYDTLAHRLRELAFLNKGIKISLTDERYDKKEMFHYEGGLRSFVLYLNRNKEKLHQ
QPIYVDENKDGCIVEIAMQYNDGYAENIFSFANNIDTIEGGTHLAGFKSALTRVINDYARKFNYLKDIDKNLSGDDVREG
LTAVISVKLTDPQFEGQTKTKLGNGEVRGIVDTIVGQSIGSFLEENPNVGKIIIEKGLSASRAREAAKRARELTRRKSIL
ESTSLPGKLSDCSSKDPSLCEIYLVEGDSAGGSAKQGRNREFQAILPLKGKIMNVEKQRLDKILASDEIRAMITAFGAGI
GKEFDIDKIRYNRIIIMTDADVDGAHIRTLLLTFFYRYMKELIEEGHVYIAQPPLYRIYKAKKETYVYSDPELDAALLEL
GGKDTNTNIQRYKGLGEMNPEQLWETTMDPEHRTLLQVTVEDAMEADEIFTILMGTKVEPRRDFIESNADKVVNLDI

Sequences:

>Translated_637_residues
MSQENKQVYDESQIQVLEGLEAVRKRPGMYIGSTSLRGLHHLVYEIVDNSIDEALAGFCTHIEVFIHKDNSITVVDDGRG
MPVGMHSKVKKPAVEVIMTILHAGGKFGGGGYKVSGGLHGVGASVVNALSEQCEVEVRREGHIWKQKFERGITKTGLDIV
GDTEDHGTKIYFKPDIEIFDELEFEYDTLAHRLRELAFLNKGIKISLTDERYDKKEMFHYEGGLRSFVLYLNRNKEKLHQ
QPIYVDENKDGCIVEIAMQYNDGYAENIFSFANNIDTIEGGTHLAGFKSALTRVINDYARKFNYLKDIDKNLSGDDVREG
LTAVISVKLTDPQFEGQTKTKLGNGEVRGIVDTIVGQSIGSFLEENPNVGKIIIEKGLSASRAREAAKRARELTRRKSIL
ESTSLPGKLSDCSSKDPSLCEIYLVEGDSAGGSAKQGRNREFQAILPLKGKIMNVEKQRLDKILASDEIRAMITAFGAGI
GKEFDIDKIRYNRIIIMTDADVDGAHIRTLLLTFFYRYMKELIEEGHVYIAQPPLYRIYKAKKETYVYSDPELDAALLEL
GGKDTNTNIQRYKGLGEMNPEQLWETTMDPEHRTLLQVTVEDAMEADEIFTILMGTKVEPRRDFIESNADKVVNLDI
>Mature_636_residues
SQENKQVYDESQIQVLEGLEAVRKRPGMYIGSTSLRGLHHLVYEIVDNSIDEALAGFCTHIEVFIHKDNSITVVDDGRGM
PVGMHSKVKKPAVEVIMTILHAGGKFGGGGYKVSGGLHGVGASVVNALSEQCEVEVRREGHIWKQKFERGITKTGLDIVG
DTEDHGTKIYFKPDIEIFDELEFEYDTLAHRLRELAFLNKGIKISLTDERYDKKEMFHYEGGLRSFVLYLNRNKEKLHQQ
PIYVDENKDGCIVEIAMQYNDGYAENIFSFANNIDTIEGGTHLAGFKSALTRVINDYARKFNYLKDIDKNLSGDDVREGL
TAVISVKLTDPQFEGQTKTKLGNGEVRGIVDTIVGQSIGSFLEENPNVGKIIIEKGLSASRAREAAKRARELTRRKSILE
STSLPGKLSDCSSKDPSLCEIYLVEGDSAGGSAKQGRNREFQAILPLKGKIMNVEKQRLDKILASDEIRAMITAFGAGIG
KEFDIDKIRYNRIIIMTDADVDGAHIRTLLLTFFYRYMKELIEEGHVYIAQPPLYRIYKAKKETYVYSDPELDAALLELG
GKDTNTNIQRYKGLGEMNPEQLWETTMDPEHRTLLQVTVEDAMEADEIFTILMGTKVEPRRDFIESNADKVVNLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=668, Percent_Identity=26.7964071856287, Blast_Score=146, Evalue=7e-35,
Organism=Homo sapiens, GI19913406, Length=611, Percent_Identity=25.69558101473, Blast_Score=135, Evalue=2e-31,
Organism=Escherichia coli, GI48994957, Length=565, Percent_Identity=57.3451327433628, Blast_Score=634, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=630, Percent_Identity=40.4761904761905, Blast_Score=466, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17535065, Length=669, Percent_Identity=25.7100149476831, Blast_Score=155, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI212645845, Length=613, Percent_Identity=25.9380097879282, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI212645657, Length=270, Percent_Identity=26.2962962962963, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324241, Length=626, Percent_Identity=26.517571884984, Blast_Score=117, Evalue=6e-27,
Organism=Drosophila melanogaster, GI17136538, Length=681, Percent_Identity=24.2290748898678, Blast_Score=145, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 71559; Mature: 71428

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00687 ALDEHYDE_DEHYDR_GLU ; PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQENKQVYDESQIQVLEGLEAVRKRPGMYIGSTSLRGLHHLVYEIVDNSIDEALAGFCT
CCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHCHHHHHHHHHHH
HIEVFIHKDNSITVVDDGRGMPVGMHSKVKKPAVEVIMTILHAGGKFGGGGYKVSGGLHG
EEEEEEECCCEEEEEECCCCCCCCCHHHHCHHHHHHHHHHHHCCCCCCCCCEEECCCCCC
VGASVVNALSEQCEVEVRREGHIWKQKFERGITKTGLDIVGDTEDHGTKIYFKPDIEIFD
HHHHHHHHHHHHHCCHHHHCCCHHHHHHHCCCCCCCCEEECCCCCCCCEEEECCCCHHHH
ELEFEYDTLAHRLRELAFLNKGIKISLTDERYDKKEMFHYEGGLRSFVLYLNRNKEKLHQ
HCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHCCCCEEEEEEEECCHHHHHC
QPIYVDENKDGCIVEIAMQYNDGYAENIFSFANNIDTIEGGTHLAGFKSALTRVINDYAR
CCEEEECCCCCEEEEEEEECCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
KFNYLKDIDKNLSGDDVREGLTAVISVKLTDPQFEGQTKTKLGNGEVRGIVDTIVGQSIG
HHHHHHHHHCCCCCHHHHHCCEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
SFLEENPNVGKIIIEKGLSASRAREAAKRARELTRRKSILESTSLPGKLSDCSSKDPSLC
HHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEE
EIYLVEGDSAGGSAKQGRNREFQAILPLKGKIMNVEKQRLDKILASDEIRAMITAFGAGI
EEEEEECCCCCCCCCCCCCCCEEEECCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCC
GKEFDIDKIRYNRIIIMTDADVDGAHIRTLLLTFFYRYMKELIEEGHVYIAQPPLYRIYK
CCCCCCCEEEECEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHH
AKKETYVYSDPELDAALLELGGKDTNTNIQRYKGLGEMNPEQLWETTMDPEHRTLLQVTV
HCCCEEEECCCCHHHHHHHCCCCCCCCCHHHHCCCCCCCHHHHHHCCCCCCCHHEEEEEH
EDAMEADEIFTILMGTKVEPRRDFIESNADKVVNLDI
HHHHCHHHHHHHHHCCCCCHHHHHHHCCCCCEEEECC
>Mature Secondary Structure 
SQENKQVYDESQIQVLEGLEAVRKRPGMYIGSTSLRGLHHLVYEIVDNSIDEALAGFCT
CCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHCHHHHHHHHHHH
HIEVFIHKDNSITVVDDGRGMPVGMHSKVKKPAVEVIMTILHAGGKFGGGGYKVSGGLHG
EEEEEEECCCEEEEEECCCCCCCCCHHHHCHHHHHHHHHHHHCCCCCCCCCEEECCCCCC
VGASVVNALSEQCEVEVRREGHIWKQKFERGITKTGLDIVGDTEDHGTKIYFKPDIEIFD
HHHHHHHHHHHHHCCHHHHCCCHHHHHHHCCCCCCCCEEECCCCCCCCEEEECCCCHHHH
ELEFEYDTLAHRLRELAFLNKGIKISLTDERYDKKEMFHYEGGLRSFVLYLNRNKEKLHQ
HCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHCCCCEEEEEEEECCHHHHHC
QPIYVDENKDGCIVEIAMQYNDGYAENIFSFANNIDTIEGGTHLAGFKSALTRVINDYAR
CCEEEECCCCCEEEEEEEECCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
KFNYLKDIDKNLSGDDVREGLTAVISVKLTDPQFEGQTKTKLGNGEVRGIVDTIVGQSIG
HHHHHHHHHCCCCCHHHHHCCEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
SFLEENPNVGKIIIEKGLSASRAREAAKRARELTRRKSILESTSLPGKLSDCSSKDPSLC
HHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEE
EIYLVEGDSAGGSAKQGRNREFQAILPLKGKIMNVEKQRLDKILASDEIRAMITAFGAGI
EEEEEECCCCCCCCCCCCCCCEEEECCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCC
GKEFDIDKIRYNRIIIMTDADVDGAHIRTLLLTFFYRYMKELIEEGHVYIAQPPLYRIYK
CCCCCCCEEEECEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHH
AKKETYVYSDPELDAALLELGGKDTNTNIQRYKGLGEMNPEQLWETTMDPEHRTLLQVTV
HCCCEEEECCCCHHHHHHHCCCCCCCCCHHHHCCCCCCCHHHHHHCCCCCCCHHEEEEEH
EDAMEADEIFTILMGTKVEPRRDFIESNADKVVNLDI
HHHHCHHHHHHHHHCCCCCHHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]