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The map label for this gene is ispG

Identifier: 170756217

GI number: 170756217

Start: 2546167

End: 2547216

Strand: Reverse

Name: ispG

Synonym: CLD_2216

Alternate gene names: 170756217

Gene position: 2547216-2546167 (Counterclockwise)

Preceding gene: 170756743

Following gene: 170754900

Centisome position: 64.35

GC content: 32.48

Gene sequence:

>1050_bases
ATGATGAGAAAATCAACTAAAAAAGTAAAAGTAGGAAGTATATATGTAGGAGGAGATTCTCCTATATCCATACAATCCAT
GACAAATACAGATACTAGAGATGTTAAAAGTACACTAAATCAAATAAATAAGTTAGAAAAAATAGGCTGTGATATAATAA
GATGCGCAGTACCAGATATAGAAGCTAGTGAAGCTTTAAAAATAATAACCAAGGAATCAAAAATTCCAGTGGTGGCAGAT
ATACATTTTGATCATAAATTAGCCTTAGAATCTATAAAAAATGGAGTAGATGCCTTAAGAATAAACCCAGGAAATATAGG
GTCCATGGAAAGGGTTAAGATGGTAGCAGAAGCCGCAAAGGGAAAATCTATTCCTATAAGGGTAGGAGTAAACTCAGGTT
CCTTAAAAAAAGATATATTAGATAAATATGGTAGAGTATGCCCAGAAGCATTAGTAGAAAGTGCCCTTCAGCATGTGAAC
ATATTAGAAAAATGCAATTTTAATGATATAGTAATATCTATAAAGTCTTCTAGCGTTATACAAATGATAGAAAGTTATAG
ATTAATATCAGAAAAAGTAAATTATCCTTTGCATTTAGGAGTTACAGAAGCAGGAACTACCTTTAGAGGAACTATAAAAT
CTAGTGTAGGTATAGGTACCCTGCTCGCAGAAGGTATAGGAGATACTATAAGAGTATCTATTACAGGAGATCCGCTAGAA
GAAATTAAAATAGGAAAAGAAATATTGAGATCCTTAGGATATGTAAATGAAGGAATAGAATTTGTATCTTGTCCAACTTG
TGGAAGAACTAATATAGATTTAATATCTATAGCCCAGGAGGTAGAAAAAAGGCTTTCAAACTGTAATAAGAATATTAAAG
TAGCGGTAATGGGATGCGTAGTTAATGGGCCTGGGGAAGCAAGGGAAGCAGATATAGGTATAGCAGGAGGCAAAGGAGAA
GGGCTTATATTCAAAAAGGGAGAGGTTATAAAAAAAGTTAAAGAAGAGAATATAATAGCTGAACTTATAAAAGAAATAGA
AAAGATGTAA

Upstream 100 bases:

>100_bases
AAATTTCATCGAAATCTAAGGATAACTTTATAAAAAACTTTGCTTAACATTATGAATTCATTGATTTTATTAGGGAAGTT
TAAAACTAGGAGATGAAACA

Downstream 100 bases:

>100_bases
TTTGTGAATACTTTAGTTTATTTAAATTAATTTGAATTTCAACATACATAGAAGATAGAGTATATAGAAGGTTCTTAGCT
TTAGATAATAGAGTTTTAAC

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MMRKSTKKVKVGSIYVGGDSPISIQSMTNTDTRDVKSTLNQINKLEKIGCDIIRCAVPDIEASEALKIITKESKIPVVAD
IHFDHKLALESIKNGVDALRINPGNIGSMERVKMVAEAAKGKSIPIRVGVNSGSLKKDILDKYGRVCPEALVESALQHVN
ILEKCNFNDIVISIKSSSVIQMIESYRLISEKVNYPLHLGVTEAGTTFRGTIKSSVGIGTLLAEGIGDTIRVSITGDPLE
EIKIGKEILRSLGYVNEGIEFVSCPTCGRTNIDLISIAQEVEKRLSNCNKNIKVAVMGCVVNGPGEAREADIGIAGGKGE
GLIFKKGEVIKKVKEENIIAELIKEIEKM

Sequences:

>Translated_349_residues
MMRKSTKKVKVGSIYVGGDSPISIQSMTNTDTRDVKSTLNQINKLEKIGCDIIRCAVPDIEASEALKIITKESKIPVVAD
IHFDHKLALESIKNGVDALRINPGNIGSMERVKMVAEAAKGKSIPIRVGVNSGSLKKDILDKYGRVCPEALVESALQHVN
ILEKCNFNDIVISIKSSSVIQMIESYRLISEKVNYPLHLGVTEAGTTFRGTIKSSVGIGTLLAEGIGDTIRVSITGDPLE
EIKIGKEILRSLGYVNEGIEFVSCPTCGRTNIDLISIAQEVEKRLSNCNKNIKVAVMGCVVNGPGEAREADIGIAGGKGE
GLIFKKGEVIKKVKEENIIAELIKEIEKM
>Mature_349_residues
MMRKSTKKVKVGSIYVGGDSPISIQSMTNTDTRDVKSTLNQINKLEKIGCDIIRCAVPDIEASEALKIITKESKIPVVAD
IHFDHKLALESIKNGVDALRINPGNIGSMERVKMVAEAAKGKSIPIRVGVNSGSLKKDILDKYGRVCPEALVESALQHVN
ILEKCNFNDIVISIKSSSVIQMIESYRLISEKVNYPLHLGVTEAGTTFRGTIKSSVGIGTLLAEGIGDTIRVSITGDPLE
EIKIGKEILRSLGYVNEGIEFVSCPTCGRTNIDLISIAQEVEKRLSNCNKNIKVAVMGCVVNGPGEAREADIGIAGGKGE
GLIFKKGEVIKKVKEENIIAELIKEIEKM

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=342, Percent_Identity=47.0760233918129, Blast_Score=361, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 37758; Mature: 37758

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRKSTKKVKVGSIYVGGDSPISIQSMTNTDTRDVKSTLNQINKLEKIGCDIIRCAVPDI
CCCCCCCEEEEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCC
EASEALKIITKESKIPVVADIHFDHKLALESIKNGVDALRINPGNIGSMERVKMVAEAAK
CHHHHHHHEECCCCCCEEEEECCCHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHC
GKSIPIRVGVNSGSLKKDILDKYGRVCPEALVESALQHVNILEKCNFNDIVISIKSSSVI
CCCCEEEEECCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHH
QMIESYRLISEKVNYPLHLGVTEAGTTFRGTIKSSVGIGTLLAEGIGDTIRVSITGDPLE
HHHHHHHHHHHHCCCCEEEEEECCCCEEEEEHHCCCCHHHHHHHCCCCEEEEEECCCCHH
EIKIGKEILRSLGYVNEGIEFVSCPTCGRTNIDLISIAQEVEKRLSNCNKNIKVAVMGCV
HHHHHHHHHHHHCCHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEE
VNGPGEAREADIGIAGGKGEGLIFKKGEVIKKVKEENIIAELIKEIEKM
ECCCCCCCCCCEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMRKSTKKVKVGSIYVGGDSPISIQSMTNTDTRDVKSTLNQINKLEKIGCDIIRCAVPDI
CCCCCCCEEEEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCC
EASEALKIITKESKIPVVADIHFDHKLALESIKNGVDALRINPGNIGSMERVKMVAEAAK
CHHHHHHHEECCCCCCEEEEECCCHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHC
GKSIPIRVGVNSGSLKKDILDKYGRVCPEALVESALQHVNILEKCNFNDIVISIKSSSVI
CCCCEEEEECCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHH
QMIESYRLISEKVNYPLHLGVTEAGTTFRGTIKSSVGIGTLLAEGIGDTIRVSITGDPLE
HHHHHHHHHHHHCCCCEEEEEECCCCEEEEEHHCCCCHHHHHHHCCCCEEEEEECCCCHH
EIKIGKEILRSLGYVNEGIEFVSCPTCGRTNIDLISIAQEVEKRLSNCNKNIKVAVMGCV
HHHHHHHHHHHHCCHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEE
VNGPGEAREADIGIAGGKGEGLIFKKGEVIKKVKEENIIAELIKEIEKM
ECCCCCCCCCCEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]