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The map label for this gene is pucF [H]

Identifier: 170756045

GI number: 170756045

Start: 751368

End: 752591

Strand: Reverse

Name: pucF [H]

Synonym: CLD_0128

Alternate gene names: 170756045

Gene position: 752591-751368 (Counterclockwise)

Preceding gene: 170754743

Following gene: 170755017

Centisome position: 19.01

GC content: 34.31

Gene sequence:

>1224_bases
ATGTTAAGTTGTAATAAAAAAAGATTAGAAGATAAAATTATTACCTTCAGTAAATTCGGCGCTACAGAAAAAGGTGGAAT
AACAAGATTATCTTTATCAAAGCCAGCCCTTGAAGCAAGAGCAGAATTTTCAAAAAGAATGAATGGACTAGGAGCAACTA
TAACAACAGATGATATGGGAAATATGTATGCTACTTTCAAAGGATCTGAAGAAAACCTTCCCCATATTGCAATGGGATCG
CATTGTGATTCAGTGGTACAAGGTGGAAATTATGATGGTATTTTAGGGGTACTTACAGCAATGGAAGTAGCTGAAACTAT
AGTTACAGAAAAAATTCCCCATCGTCATCCAATTACAGTTATGATTTGGACTAATGAAGAAGGCGCTCGTTTTGACCCTG
CTATGATGTCCTCAGGTGTTATTACAGGTAAATTTGATAAAGCAGAAATGTTAGATTCTAAGGATAAAGAGGGTATATCC
TTTGGTGAGTCACTAGATGCAAGCGGATATAAAGGTGAAGAAAAAAACAGAATAAATCCCAAAGATTATATGGCTCTTCT
AGAACTTCATATTGAACAAGGTCCAGTTCTAGAATCTTCAAAAATAGATATAGGTGTTGTAGAAGGTGTTGTTGGAATGG
TAAATTATGAGTTTGAATTTATTGGCCAAGCCGGTCATGCTGGAACAGTTTCACAAAAAATGAGAAAGGATGCTCTTTTA
GCAGCTTCTGAAGCCATTCAATATTTGCATAAAGAACTTGATAAGCTGGATGATAACTTAGTGTATACTACGGGTAGAAT
AATTTGTTTCCCTAATATACATACGATTATACCGGATAATGTTAAATTCACATTAGATGCAAGACATCAAGATCCAAAAG
TAATACAACGAGTGGTTAAAATTATCGAAAATATCCCCAAAGAATTAGGAAATTGTAAAGTCACTTACAGGAAATTATGG
TCACGTAAAACTATTAGTTTCAATAATGAATTGATTAATTTCGTGGAGAAAAATGCTAATCTTTACGGTTACTCTAATAT
GAGAATATACAGCGGTCCCGGCCATGATGCCCAATTTGCAGCAGATATGTTACCTACAACCATGATTTTCGTTCCAAGTA
TTGGTGGCCACAGTCATTGTGAAATAGAAAAGACACCACTTGATAATTGTTTAAAAGGAGCTAATGTGCTTCTACAAACA
ATTCTAGATATAGATAAAAAATAA

Upstream 100 bases:

>100_bases
TTTTAGTACGTTGTACACTATATATAAGAATAAATGTATAGTGAGGGTTTACTTCTTAGAATACATTCTCTCACTAAAAA
TAAAAGATGGGGTGTGTTGT

Downstream 100 bases:

>100_bases
ATATTCACTCATATGGAATCCCAGCAACAGCTTTATTATTACTGGGATTCCACATTTATATTAGTTAATTTTAAATTTTA
CTTATCTAATCCCCTTTTGT

Product: allantoate amidohydrolase

Products: NA

Alternate protein names: Allantoate deiminase [H]

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MLSCNKKRLEDKIITFSKFGATEKGGITRLSLSKPALEARAEFSKRMNGLGATITTDDMGNMYATFKGSEENLPHIAMGS
HCDSVVQGGNYDGILGVLTAMEVAETIVTEKIPHRHPITVMIWTNEEGARFDPAMMSSGVITGKFDKAEMLDSKDKEGIS
FGESLDASGYKGEEKNRINPKDYMALLELHIEQGPVLESSKIDIGVVEGVVGMVNYEFEFIGQAGHAGTVSQKMRKDALL
AASEAIQYLHKELDKLDDNLVYTTGRIICFPNIHTIIPDNVKFTLDARHQDPKVIQRVVKIIENIPKELGNCKVTYRKLW
SRKTISFNNELINFVEKNANLYGYSNMRIYSGPGHDAQFAADMLPTTMIFVPSIGGHSHCEIEKTPLDNCLKGANVLLQT
ILDIDKK

Sequences:

>Translated_407_residues
MLSCNKKRLEDKIITFSKFGATEKGGITRLSLSKPALEARAEFSKRMNGLGATITTDDMGNMYATFKGSEENLPHIAMGS
HCDSVVQGGNYDGILGVLTAMEVAETIVTEKIPHRHPITVMIWTNEEGARFDPAMMSSGVITGKFDKAEMLDSKDKEGIS
FGESLDASGYKGEEKNRINPKDYMALLELHIEQGPVLESSKIDIGVVEGVVGMVNYEFEFIGQAGHAGTVSQKMRKDALL
AASEAIQYLHKELDKLDDNLVYTTGRIICFPNIHTIIPDNVKFTLDARHQDPKVIQRVVKIIENIPKELGNCKVTYRKLW
SRKTISFNNELINFVEKNANLYGYSNMRIYSGPGHDAQFAADMLPTTMIFVPSIGGHSHCEIEKTPLDNCLKGANVLLQT
ILDIDKK
>Mature_407_residues
MLSCNKKRLEDKIITFSKFGATEKGGITRLSLSKPALEARAEFSKRMNGLGATITTDDMGNMYATFKGSEENLPHIAMGS
HCDSVVQGGNYDGILGVLTAMEVAETIVTEKIPHRHPITVMIWTNEEGARFDPAMMSSGVITGKFDKAEMLDSKDKEGIS
FGESLDASGYKGEEKNRINPKDYMALLELHIEQGPVLESSKIDIGVVEGVVGMVNYEFEFIGQAGHAGTVSQKMRKDALL
AASEAIQYLHKELDKLDDNLVYTTGRIICFPNIHTIIPDNVKFTLDARHQDPKVIQRVVKIIENIPKELGNCKVTYRKLW
SRKTISFNNELINFVEKNANLYGYSNMRIYSGPGHDAQFAADMLPTTMIFVPSIGGHSHCEIEKTPLDNCLKGANVLLQT
ILDIDKK

Specific function: Involved in the anaerobic utilization of allantoin. Converts allantoate to (S)-ureidoglycolate and ammonia [H]

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI1786726, Length=401, Percent_Identity=32.1695760598504, Blast_Score=206, Evalue=3e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010158
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF01546 Peptidase_M20 [H]

EC number: 3.5.3.- [C]

Molecular weight: Translated: 45095; Mature: 45095

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSCNKKRLEDKIITFSKFGATEKGGITRLSLSKPALEARAEFSKRMNGLGATITTDDMG
CCCCCHHHHHHHHEEHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCC
NMYATFKGSEENLPHIAMGSHCDSVVQGGNYDGILGVLTAMEVAETIVTEKIPHRHPITV
CEEEEEECCCCCCCEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEE
MIWTNEEGARFDPAMMSSGVITGKFDKAEMLDSKDKEGISFGESLDASGYKGEEKNRINP
EEEECCCCCCCCHHHHHCCCEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
KDYMALLELHIEQGPVLESSKIDIGVVEGVVGMVNYEFEFIGQAGHAGTVSQKMRKDALL
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHEEEEEEEECCCCCCHHHHHHHHHHHH
AASEAIQYLHKELDKLDDNLVYTTGRIICFPNIHTIIPDNVKFTLDARHQDPKVIQRVVK
HHHHHHHHHHHHHHHCCCCEEEECCEEEEECCCCEECCCCEEEEEECCCCCHHHHHHHHH
IIENIPKELGNCKVTYRKLWSRKTISFNNELINFVEKNANLYGYSNMRIYSGPGHDAQFA
HHHHHHHHHCCCEEEHHHHHCCCCCCCCHHHHHHHHHCCCEEEECCEEEECCCCCCHHHH
ADMLPTTMIFVPSIGGHSHCEIEKTPLDNCLKGANVLLQTILDIDKK
HHHCCEEEEEEECCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLSCNKKRLEDKIITFSKFGATEKGGITRLSLSKPALEARAEFSKRMNGLGATITTDDMG
CCCCCHHHHHHHHEEHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCC
NMYATFKGSEENLPHIAMGSHCDSVVQGGNYDGILGVLTAMEVAETIVTEKIPHRHPITV
CEEEEEECCCCCCCEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEE
MIWTNEEGARFDPAMMSSGVITGKFDKAEMLDSKDKEGISFGESLDASGYKGEEKNRINP
EEEECCCCCCCCHHHHHCCCEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
KDYMALLELHIEQGPVLESSKIDIGVVEGVVGMVNYEFEFIGQAGHAGTVSQKMRKDALL
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHEEEEEEEECCCCCCHHHHHHHHHHHH
AASEAIQYLHKELDKLDDNLVYTTGRIICFPNIHTIIPDNVKFTLDARHQDPKVIQRVVK
HHHHHHHHHHHHHHHCCCCEEEECCEEEEECCCCEECCCCEEEEEECCCCCHHHHHHHHH
IIENIPKELGNCKVTYRKLWSRKTISFNNELINFVEKNANLYGYSNMRIYSGPGHDAQFA
HHHHHHHHHCCCEEEHHHHHCCCCCCCCHHHHHHHHHCCCEEEECCEEEECCCCCCHHHH
ADMLPTTMIFVPSIGGHSHCEIEKTPLDNCLKGANVLLQTILDIDKK
HHHCCEEEEEEECCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]