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The map label for this gene is fliN

Identifier: 170756013

GI number: 170756013

Start: 2912728

End: 2913888

Strand: Reverse

Name: fliN

Synonym: CLD_1831

Alternate gene names: 170756013

Gene position: 2913888-2912728 (Counterclockwise)

Preceding gene: 170756595

Following gene: 170754843

Centisome position: 73.62

GC content: 33.76

Gene sequence:

>1161_bases
ATGAGTAACGGGTTTCTTTCACAGGAAGAAATAAATGCTCTTCTAAATGGAGATGGAAGCCAAGGGGAGGAAGCAAAAAA
AGAAGCAACAGATACTGAACTACCAGAATTACCTGATCTTACAGATCTTGAAAAAGATCTTCTAGGAGAAGTGGGAAATA
TATCTATGGGTTCAGCCTCCACAGCGCTTTCAACTATAATAAATCAGCAGGTTAATATAACAACACCTGTGGTAAGTGTT
ACAACTTTAGAAAGATTAAAAAATTCTTTTGAAATTCCTAATATAGCGCTAGAGGTAAAATATACCAGTGGTATAGTAGG
AGAAAACCTATTAGTTATGAAAATTGACGATGCCTCTGTAATATCAAATTTAATGATGGGTGGAAATGGGATTGTTGAAG
GAGAAATAAAAGAGCTATCAGAAATAGAAATAAGTGCTGTATCAGAAGCTATGAATCAAATGATAGGTTCAGCAGCTACA
TCTATGGCCACTATGTTTGCAAGAGAAGTAAATATATCTCCACCACTATCAAAAATTTGGTCAGATTTAACAGCACCATT
AGCAGAAGGAATAAGTGATGATGAACCAGTGGTTTTAGTATCCTTTTCTTTAAATATAGGTGAGTTAGTAGATAGTAGTA
TAATGCAATTATTACCTATGAAAACAGCTAAAAAAATAGTTGCGATTATGATGGGAGAAGAAAGTATGGATGAGCCATCA
GTAGAAAACAATTCAACTCAAGCATCAACTGTAGAGGAGAAAAAGCCTGCTAAAGCAGTAGAAGCACCAAAGCAAGAAAT
TGTATATGAAAAACCAATAGAAAGAGAAGCTCCAAAGGTAGAAGTAAAGCAAGCTTCATTTGAACCATTATCTGTAAGAG
AATCTGCAACTACTATGCCTAATAATATAGACCTAATATTAGATGTACCACTTCAAATATCTGTAGTATTAGGAAGAACA
AAAATGAATGTAAAGGATATATTAAATTTAGGCAATGGTTCTTTAATAGAATTAGACAAGCTAGCAGAAGAGCCAGTAGA
AATACAAGTAAATGGTAAAAAAGTAGCCTATGGAGAAGTAGTTGTAGTAGACGAAAACTTTGGGGTAAGAATAACTAGTA
TAGTTAGCAGTCAAGAAAGAATAAAATCTTTAGGAAAGTAG

Upstream 100 bases:

>100_bases
TTAATAGAAGAAGAACCAGGTTATTATGCTAAACCAGGAATTATAGGCAAGAATATAGGGTTACAGATACTAGATCAAAT
AGATAAGGATGTGGAAAGTT

Downstream 100 bases:

>100_bases
AAAGAGTTATATAGTAATGTGTCTCAGAATAAACTAGGTTTAATTTTAAAACTGTAAAGATAAAAAATACATTCATAAAC
TAATTTAATTAACTAATAAA

Product: flagellar motor switch protein

Products: NA

Alternate protein names: CheY-P phosphatase FliY [H]

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MSNGFLSQEEINALLNGDGSQGEEAKKEATDTELPELPDLTDLEKDLLGEVGNISMGSASTALSTIINQQVNITTPVVSV
TTLERLKNSFEIPNIALEVKYTSGIVGENLLVMKIDDASVISNLMMGGNGIVEGEIKELSEIEISAVSEAMNQMIGSAAT
SMATMFAREVNISPPLSKIWSDLTAPLAEGISDDEPVVLVSFSLNIGELVDSSIMQLLPMKTAKKIVAIMMGEESMDEPS
VENNSTQASTVEEKKPAKAVEAPKQEIVYEKPIEREAPKVEVKQASFEPLSVRESATTMPNNIDLILDVPLQISVVLGRT
KMNVKDILNLGNGSLIELDKLAEEPVEIQVNGKKVAYGEVVVVDENFGVRITSIVSSQERIKSLGK

Sequences:

>Translated_386_residues
MSNGFLSQEEINALLNGDGSQGEEAKKEATDTELPELPDLTDLEKDLLGEVGNISMGSASTALSTIINQQVNITTPVVSV
TTLERLKNSFEIPNIALEVKYTSGIVGENLLVMKIDDASVISNLMMGGNGIVEGEIKELSEIEISAVSEAMNQMIGSAAT
SMATMFAREVNISPPLSKIWSDLTAPLAEGISDDEPVVLVSFSLNIGELVDSSIMQLLPMKTAKKIVAIMMGEESMDEPS
VENNSTQASTVEEKKPAKAVEAPKQEIVYEKPIEREAPKVEVKQASFEPLSVRESATTMPNNIDLILDVPLQISVVLGRT
KMNVKDILNLGNGSLIELDKLAEEPVEIQVNGKKVAYGEVVVVDENFGVRITSIVSSQERIKSLGK
>Mature_385_residues
SNGFLSQEEINALLNGDGSQGEEAKKEATDTELPELPDLTDLEKDLLGEVGNISMGSASTALSTIINQQVNITTPVVSVT
TLERLKNSFEIPNIALEVKYTSGIVGENLLVMKIDDASVISNLMMGGNGIVEGEIKELSEIEISAVSEAMNQMIGSAATS
MATMFAREVNISPPLSKIWSDLTAPLAEGISDDEPVVLVSFSLNIGELVDSSIMQLLPMKTAKKIVAIMMGEESMDEPSV
ENNSTQASTVEEKKPAKAVEAPKQEIVYEKPIEREAPKVEVKQASFEPLSVRESATTMPNNIDLILDVPLQISVVLGRTK
MNVKDILNLGNGSLIELDKLAEEPVEIQVNGKKVAYGEVVVVDENFGVRITSIVSSQERIKSLGK

Specific function: Component of the flagellar switch. Binds CheY-P and increases its hydrolysis rate in vitro. May function constitutively to remove CheY-P around the flagellar switch to maintain an optimal level of CheY-P whereas CheC may function after addition of an attr

COG id: COG1776

COG function: function code NT; Chemotaxis protein CheC, inhibitor of MCP methylation

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=The switch is localized at the base of the flagellar motor, in or near the C-ring, attached to the MS-ring (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliN/MopA/SpaO family [H]

Homologues:

Organism=Escherichia coli, GI1788256, Length=85, Percent_Identity=49.4117647058824, Blast_Score=95, Evalue=9e-21,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007597
- InterPro:   IPR012826
- InterPro:   IPR001172
- InterPro:   IPR001543 [H]

Pfam domain/function: PF04509 CheC; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 41546; Mature: 41415

Theoretical pI: Translated: 4.09; Mature: 4.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNGFLSQEEINALLNGDGSQGEEAKKEATDTELPELPDLTDLEKDLLGEVGNISMGSAS
CCCCCCCHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHH
TALSTIINQQVNITTPVVSVTTLERLKNSFEIPNIALEVKYTSGIVGENLLVMKIDDASV
HHHHHHHCCCCEEECCEEHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEEEECCHHH
ISNLMMGGNGIVEGEIKELSEIEISAVSEAMNQMIGSAATSMATMFAREVNISPPLSKIW
HHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
SDLTAPLAEGISDDEPVVLVSFSLNIGELVDSSIMQLLPMKTAKKIVAIMMGEESMDEPS
HHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCC
VENNSTQASTVEEKKPAKAVEAPKQEIVYEKPIEREAPKVEVKQASFEPLSVRESATTMP
CCCCCCCHHHHHHHCCHHHHHCCHHHHHCCCCCCCCCCCEEEECCCCCCCCCCHHCCCCC
NNIDLILDVPLQISVVLGRTKMNVKDILNLGNGSLIELDKLAEEPVEIQVNGKKVAYGEV
CCCEEEEECCEEEEEEECCCCCCHHHHHHCCCCCEEEHHHHHCCCEEEEECCEEEEEEEE
VVVDENFGVRITSIVSSQERIKSLGK
EEEECCCCEEEEHHHHHHHHHHHHCC
>Mature Secondary Structure 
SNGFLSQEEINALLNGDGSQGEEAKKEATDTELPELPDLTDLEKDLLGEVGNISMGSAS
CCCCCCHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHH
TALSTIINQQVNITTPVVSVTTLERLKNSFEIPNIALEVKYTSGIVGENLLVMKIDDASV
HHHHHHHCCCCEEECCEEHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEEEECCHHH
ISNLMMGGNGIVEGEIKELSEIEISAVSEAMNQMIGSAATSMATMFAREVNISPPLSKIW
HHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
SDLTAPLAEGISDDEPVVLVSFSLNIGELVDSSIMQLLPMKTAKKIVAIMMGEESMDEPS
HHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCC
VENNSTQASTVEEKKPAKAVEAPKQEIVYEKPIEREAPKVEVKQASFEPLSVRESATTMP
CCCCCCCHHHHHHHCCHHHHHCCHHHHHCCCCCCCCCCCEEEECCCCCCCCCCHHCCCCC
NNIDLILDVPLQISVVLGRTKMNVKDILNLGNGSLIELDKLAEEPVEIQVNGKKVAYGEV
CCCEEEEECCEEEEEEECCCCCCHHHHHHCCCCCEEEHHHHHCCCEEEEECCEEEEEEEE
VVVDENFGVRITSIVSSQERIKSLGK
EEEECCCCEEEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1447979; 9384377; 1898932 [H]