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The map label for this gene is glyA

Identifier: 170755750

GI number: 170755750

Start: 2772174

End: 2773415

Strand: Reverse

Name: glyA

Synonym: CLD_1970

Alternate gene names: 170755750

Gene position: 2773415-2772174 (Counterclockwise)

Preceding gene: 170756194

Following gene: 170755451

Centisome position: 70.07

GC content: 30.84

Gene sequence:

>1242_bases
ATGGACTTTACAAATTTAAAAAACACAGATCCAGAGCTATTAGACATGATAAAGAAAGAAGAGGAAAGACAAGAATATAA
TATCGAATTAATAGCTTCAGAAAATTTTACTAGTTTATCTGTAATGGAGGCTATGGGATCTTTATTGACAAATAAATATG
CAGAAGGATACCCACATAAAAGATATTATGGAGGATGTGAATTTGTAGATGAGGTAGAGGATTTAGCAAGAGAAAGATTA
AAAAAATTATTTGTTGCAGAACATGCCAATGTACAACCACATTCTGGTTCACAAGCCAATATGGCAGTTTATATGTCTGT
GCTTCAGACTGGTGATACTATATTAGGTATGGATTTATCCCATGGCGGACATTTAACCCATGGAAGTCCAGTAAATTTTT
CAGGAAAATTATATAATTTTATATCCTATGGTGTAGATAAAGAAACAGAAACTATAGATTATGATCAATTAAAGAAAATA
GCCTTAGAAAATAGACCTAAAATGATAGTATCTGGAGCTAGTGCTTATCCTAGAATTATAGATTTTGAAAAGATAAGAGA
AATTTGTGATGAAATAGATGCTTATATGATGGTAGATATGGCTCATATAGCAGGATTAGTAGCTACAGGAATACATCCAT
CACCAGTACCTTATGCAGATTTTGTTACAACAACTACTCATAAAACTTTAAGAGGACCTAGAGGTGGGGCTATTTTATGT
AAAGAAAAATATGCAAAAGCAGTAGATAAAGCTATATTCCCAGGCATTCAAGGGGGACCATTAATGCATACTATAGCAGC
AAAAGCCGTTTGTTTTGGGGAAGCTTTAAGAGAAGATTATAAAGAATATATGCAGCAAGTTGTTAAAAATACTAAAGTTT
TAGGAGAAGAATTAAAAAATTATGGATTTAGACTAATATCTGGTGGTACAGATAACCATTTATTACTAATAGATTTAACT
AATAAAAATATAACAGGAAAAGATGCAGAAAAACTTTTAGATTCAGTAGGAATTACTGTGAATAAAAATACAATACCTTT
TGAAACTTTAAGCCCTTTTATAACTAGTGGAATTAGAATAGGAACTCCAGCGGTAACTACGAGAGGATTTAAGGAAGAGG
AAATGAAAAAAATAGCCTATTTCATGAATTATTCTATAGAACATAGGGAAGAGAATTTATCTCAAATAAAAGAGCAAATA
AAAGAAATTTGTAAAAAATACCCATTGCATCAAAATGCATAA

Upstream 100 bases:

>100_bases
AATTTTTGTAGAAACATAAAAGTTAAATTTTATTGATTATTTTAAATTTTACAACTATAATTTATAATTAATATATGTTT
ATTTTTAGGAGCGTGATAAT

Downstream 100 bases:

>100_bases
AAAATGTAGAATTTACACATAATAGATAGGGCTTATGTCCCAAAACAGGAGCATAAGCCCATATTCTTGTGATATATTGT
AGAAAAACTATATAAATTCA

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MDFTNLKNTDPELLDMIKKEEERQEYNIELIASENFTSLSVMEAMGSLLTNKYAEGYPHKRYYGGCEFVDEVEDLARERL
KKLFVAEHANVQPHSGSQANMAVYMSVLQTGDTILGMDLSHGGHLTHGSPVNFSGKLYNFISYGVDKETETIDYDQLKKI
ALENRPKMIVSGASAYPRIIDFEKIREICDEIDAYMMVDMAHIAGLVATGIHPSPVPYADFVTTTTHKTLRGPRGGAILC
KEKYAKAVDKAIFPGIQGGPLMHTIAAKAVCFGEALREDYKEYMQQVVKNTKVLGEELKNYGFRLISGGTDNHLLLIDLT
NKNITGKDAEKLLDSVGITVNKNTIPFETLSPFITSGIRIGTPAVTTRGFKEEEMKKIAYFMNYSIEHREENLSQIKEQI
KEICKKYPLHQNA

Sequences:

>Translated_413_residues
MDFTNLKNTDPELLDMIKKEEERQEYNIELIASENFTSLSVMEAMGSLLTNKYAEGYPHKRYYGGCEFVDEVEDLARERL
KKLFVAEHANVQPHSGSQANMAVYMSVLQTGDTILGMDLSHGGHLTHGSPVNFSGKLYNFISYGVDKETETIDYDQLKKI
ALENRPKMIVSGASAYPRIIDFEKIREICDEIDAYMMVDMAHIAGLVATGIHPSPVPYADFVTTTTHKTLRGPRGGAILC
KEKYAKAVDKAIFPGIQGGPLMHTIAAKAVCFGEALREDYKEYMQQVVKNTKVLGEELKNYGFRLISGGTDNHLLLIDLT
NKNITGKDAEKLLDSVGITVNKNTIPFETLSPFITSGIRIGTPAVTTRGFKEEEMKKIAYFMNYSIEHREENLSQIKEQI
KEICKKYPLHQNA
>Mature_413_residues
MDFTNLKNTDPELLDMIKKEEERQEYNIELIASENFTSLSVMEAMGSLLTNKYAEGYPHKRYYGGCEFVDEVEDLARERL
KKLFVAEHANVQPHSGSQANMAVYMSVLQTGDTILGMDLSHGGHLTHGSPVNFSGKLYNFISYGVDKETETIDYDQLKKI
ALENRPKMIVSGASAYPRIIDFEKIREICDEIDAYMMVDMAHIAGLVATGIHPSPVPYADFVTTTTHKTLRGPRGGAILC
KEKYAKAVDKAIFPGIQGGPLMHTIAAKAVCFGEALREDYKEYMQQVVKNTKVLGEELKNYGFRLISGGTDNHLLLIDLT
NKNITGKDAEKLLDSVGITVNKNTIPFETLSPFITSGIRIGTPAVTTRGFKEEEMKKIAYFMNYSIEHREENLSQIKEQI
KEICKKYPLHQNA

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI19923315, Length=407, Percent_Identity=45.2088452088452, Blast_Score=368, Evalue=1e-102,
Organism=Homo sapiens, GI261862352, Length=407, Percent_Identity=45.2088452088452, Blast_Score=368, Evalue=1e-102,
Organism=Homo sapiens, GI261862350, Length=407, Percent_Identity=45.2088452088452, Blast_Score=368, Evalue=1e-102,
Organism=Homo sapiens, GI261862348, Length=407, Percent_Identity=45.2088452088452, Blast_Score=368, Evalue=1e-102,
Organism=Homo sapiens, GI261862346, Length=403, Percent_Identity=44.9131513647643, Blast_Score=350, Evalue=1e-96,
Organism=Homo sapiens, GI22547186, Length=407, Percent_Identity=44.963144963145, Blast_Score=336, Evalue=2e-92,
Organism=Homo sapiens, GI22547189, Length=391, Percent_Identity=43.7340153452685, Blast_Score=309, Evalue=3e-84,
Organism=Escherichia coli, GI1788902, Length=411, Percent_Identity=58.1508515815085, Blast_Score=478, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI25144732, Length=406, Percent_Identity=47.0443349753695, Blast_Score=372, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI25144729, Length=406, Percent_Identity=47.0443349753695, Blast_Score=372, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6319739, Length=416, Percent_Identity=44.4711538461538, Blast_Score=371, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6323087, Length=427, Percent_Identity=44.2622950819672, Blast_Score=359, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24640005, Length=407, Percent_Identity=45.945945945946, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI221329721, Length=407, Percent_Identity=45.945945945946, Blast_Score=377, Evalue=1e-105,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 46328; Mature: 46328

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFTNLKNTDPELLDMIKKEEERQEYNIELIASENFTSLSVMEAMGSLLTNKYAEGYPHK
CCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCC
RYYGGCEFVDEVEDLARERLKKLFVAEHANVQPHSGSQANMAVYMSVLQTGDTILGMDLS
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECC
HGGHLTHGSPVNFSGKLYNFISYGVDKETETIDYDQLKKIALENRPKMIVSGASAYPRII
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCC
DFEKIREICDEIDAYMMVDMAHIAGLVATGIHPSPVPYADFVTTTTHKTLRGPRGGAILC
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCHHHHCCCCCCEEEE
KEKYAKAVDKAIFPGIQGGPLMHTIAAKAVCFGEALREDYKEYMQQVVKNTKVLGEELKN
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGFRLISGGTDNHLLLIDLTNKNITGKDAEKLLDSVGITVNKNTIPFETLSPFITSGIRI
CCEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHCCCEECCCCCCHHHHHHHHHCCEEE
GTPAVTTRGFKEEEMKKIAYFMNYSIEHREENLSQIKEQIKEICKKYPLHQNA
CCCCHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDFTNLKNTDPELLDMIKKEEERQEYNIELIASENFTSLSVMEAMGSLLTNKYAEGYPHK
CCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCC
RYYGGCEFVDEVEDLARERLKKLFVAEHANVQPHSGSQANMAVYMSVLQTGDTILGMDLS
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECC
HGGHLTHGSPVNFSGKLYNFISYGVDKETETIDYDQLKKIALENRPKMIVSGASAYPRII
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCC
DFEKIREICDEIDAYMMVDMAHIAGLVATGIHPSPVPYADFVTTTTHKTLRGPRGGAILC
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCHHHHCCCCCCEEEE
KEKYAKAVDKAIFPGIQGGPLMHTIAAKAVCFGEALREDYKEYMQQVVKNTKVLGEELKN
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGFRLISGGTDNHLLLIDLTNKNITGKDAEKLLDSVGITVNKNTIPFETLSPFITSGIRI
CCEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHCCCEECCCCCCHHHHHHHHHCCEEE
GTPAVTTRGFKEEEMKKIAYFMNYSIEHREENLSQIKEQIKEICKKYPLHQNA
CCCCHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA