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The map label for this gene is splB

Identifier: 170755736

GI number: 170755736

Start: 259555

End: 260562

Strand: Direct

Name: splB

Synonym: CLD_0559

Alternate gene names: 170755736

Gene position: 259555-260562 (Clockwise)

Preceding gene: 170756505

Following gene: 170757793

Centisome position: 6.56

GC content: 28.37

Gene sequence:

>1008_bases
ATGTTTATTCCCAAAACAGTTATATTTGAGGAAAAAGCTTTAGAGTATGTTAGAGGTAAAGAAATTTTAAGAGAATTAAA
AAATAAAAATATAGAAATAAAATATTCCAAGACAGGAAGAGTAACAGGGCGGAGAGATAAAACTCCAAATGAAATGTATT
CTGAAGGTAAAGATACATTAGTAATAGGTATAAGAAAGTCTTTAGATTTTCAAAGCTGTAAGCCATCAGCTCATTACCAA
TTGCCTTTAGTAAGCGGATGTATGGGAATGTGTGAATATTGTTATTTAAATACTCAAATGGGAAAAAGACCTTACACAAA
AGTGTATGTAAATACAGAAGAGATATTAAATAAGGCTGAAAAATATTCAAGAGAAAGATTACCTGAAGTTACAGTTTTTG
AAGGAGCGGCTACATCAGATCCTATACCAGTAGAACCGTATACACATTCCTTAAAGGATACTATAGAATTCTTTGGGCAA
AAACATAATATGAAATTTAGATTTGTAACTAAATTTACAGATGTAAATAGCTTAGTTCATTTAAATCATAACAGAAATAC
TACCATTAGATTTAGTATAAATACAGATAATATAATAAGAAATTTTGAACATAGAACACCCCTGGTTATAGATAGGTTGA
AGGCAGCCGAAAAGATAGCTAAAGCTGGGTATAGTTTAGGATTTATTATAGCACCAGTGTTTATATATAGAGGTTGGGAA
AAAGAGTATTTAGATTTATTAAAAAACATAAATATTATGTTTAAAGGTTTTCCCATAGAATTTGAAGTTATATCCCATAG
ATTTACCAAAAGGGCTAAGGATAACATATTAAGTGTGTTTTCAAACACAACTTTGCCTATGGTAGAAGAGAATAGAAAAT
TTAAATATGGTCAGTTTGGATATGGAAAATATATATATAAAGATGAAGAGTTACAGAATATAAAAAAATTCTTTAGAGAT
AGTATAACAGAATTTTTTGGAGAAGATAACATTAACTACATTATATAG

Upstream 100 bases:

>100_bases
TGAAATATTTTCTTTTACCATTTCATTCATATGGATATTATATAATATTATTACATATACTTTAGAAATAAATTGCATAC
TATAACTAAGGAGTGATAGT

Downstream 100 bases:

>100_bases
AGAATTAACATTTTGTTAATTCTTTATATTCTTTACTAAAGCTTTATAATAGAGTAATATAGTTATTGTAATAACCCTTG
TATACTTATTAATTTAAATA

Product: spore photoproduct lyase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MFIPKTVIFEEKALEYVRGKEILRELKNKNIEIKYSKTGRVTGRRDKTPNEMYSEGKDTLVIGIRKSLDFQSCKPSAHYQ
LPLVSGCMGMCEYCYLNTQMGKRPYTKVYVNTEEILNKAEKYSRERLPEVTVFEGAATSDPIPVEPYTHSLKDTIEFFGQ
KHNMKFRFVTKFTDVNSLVHLNHNRNTTIRFSINTDNIIRNFEHRTPLVIDRLKAAEKIAKAGYSLGFIIAPVFIYRGWE
KEYLDLLKNINIMFKGFPIEFEVISHRFTKRAKDNILSVFSNTTLPMVEENRKFKYGQFGYGKYIYKDEELQNIKKFFRD
SITEFFGEDNINYII

Sequences:

>Translated_335_residues
MFIPKTVIFEEKALEYVRGKEILRELKNKNIEIKYSKTGRVTGRRDKTPNEMYSEGKDTLVIGIRKSLDFQSCKPSAHYQ
LPLVSGCMGMCEYCYLNTQMGKRPYTKVYVNTEEILNKAEKYSRERLPEVTVFEGAATSDPIPVEPYTHSLKDTIEFFGQ
KHNMKFRFVTKFTDVNSLVHLNHNRNTTIRFSINTDNIIRNFEHRTPLVIDRLKAAEKIAKAGYSLGFIIAPVFIYRGWE
KEYLDLLKNINIMFKGFPIEFEVISHRFTKRAKDNILSVFSNTTLPMVEENRKFKYGQFGYGKYIYKDEELQNIKKFFRD
SITEFFGEDNINYII
>Mature_335_residues
MFIPKTVIFEEKALEYVRGKEILRELKNKNIEIKYSKTGRVTGRRDKTPNEMYSEGKDTLVIGIRKSLDFQSCKPSAHYQ
LPLVSGCMGMCEYCYLNTQMGKRPYTKVYVNTEEILNKAEKYSRERLPEVTVFEGAATSDPIPVEPYTHSLKDTIEFFGQ
KHNMKFRFVTKFTDVNSLVHLNHNRNTTIRFSINTDNIIRNFEHRTPLVIDRLKAAEKIAKAGYSLGFIIAPVFIYRGWE
KEYLDLLKNINIMFKGFPIEFEVISHRFTKRAKDNILSVFSNTTLPMVEENRKFKYGQFGYGKYIYKDEELQNIKKFFRD
SITEFFGEDNINYII

Specific function: Involved in repair of UV radiation-induced DNA damage during spore germination. Can repair thymine dimer 5-thyminyl-5,6- dihydrothymine (known as spore photoproduct (SP)) by in situ monomerization of SP to two thymines [H]

COG id: COG1533

COG function: function code L; DNA repair photolyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. SPL family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =4.1.99.14 [H]

Molecular weight: Translated: 39327; Mature: 39327

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFIPKTVIFEEKALEYVRGKEILRELKNKNIEIKYSKTGRVTGRRDKTPNEMYSEGKDTL
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHCCCCEE
VIGIRKSLDFQSCKPSAHYQLPLVSGCMGMCEYCYLNTQMGKRPYTKVYVNTEEILNKAE
EEEEECCCCCCCCCCCCCEECHHHHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHHHHHH
KYSRERLPEVTVFEGAATSDPIPVEPYTHSLKDTIEFFGQKHNMKFRFVTKFTDVNSLVH
HHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCEEE
LNHNRNTTIRFSINTDNIIRNFEHRTPLVIDRLKAAEKIAKAGYSLGFIIAPVFIYRGWE
EECCCCEEEEEEECCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
KEYLDLLKNINIMFKGFPIEFEVISHRFTKRAKDNILSVFSNTTLPMVEENRKFKYGQFG
HHHHHHHHCCCEEEECCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCEECCCCC
YGKYIYKDEELQNIKKFFRDSITEFFGEDNINYII
CCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEC
>Mature Secondary Structure
MFIPKTVIFEEKALEYVRGKEILRELKNKNIEIKYSKTGRVTGRRDKTPNEMYSEGKDTL
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHCCCCEE
VIGIRKSLDFQSCKPSAHYQLPLVSGCMGMCEYCYLNTQMGKRPYTKVYVNTEEILNKAE
EEEEECCCCCCCCCCCCCEECHHHHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHHHHHH
KYSRERLPEVTVFEGAATSDPIPVEPYTHSLKDTIEFFGQKHNMKFRFVTKFTDVNSLVH
HHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCEEE
LNHNRNTTIRFSINTDNIIRNFEHRTPLVIDRLKAAEKIAKAGYSLGFIIAPVFIYRGWE
EECCCCEEEEEEECCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
KEYLDLLKNINIMFKGFPIEFEVISHRFTKRAKDNILSVFSNTTLPMVEENRKFKYGQFG
HHHHHHHHCCCEEEECCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCEECCCCC
YGKYIYKDEELQNIKKFFRDSITEFFGEDNINYII
CCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]