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The map label for this gene is ykoW [H]

Identifier: 170755524

GI number: 170755524

Start: 1348350

End: 1349609

Strand: Direct

Name: ykoW [H]

Synonym: CLD_3355

Alternate gene names: 170755524

Gene position: 1348350-1349609 (Clockwise)

Preceding gene: 170757787

Following gene: 170754410

Centisome position: 34.06

GC content: 25.71

Gene sequence:

>1260_bases
ATGAAAGAGAAAAATTTAATAAAACAAATGGAGTTATCTGTTATAGAAAAAATGGGTATAGCTTATTGTTGCTGCAAAAT
AAGTTTGAACTCATATGGTCAGTTAGAGGATTTTATTTTTATGGAATCTAATGATTATTTTAAAGATTTAACGGGTTTTA
CAAAAGAATATATAAAAAATAAAAAAGCTAAAGATATAATTGCCTTTGGAAGTCATAGAATAAATATAATGAAAGCCTTA
GAAAAAATTTTATTATATAAAGAAGAAAAAATAGAATTTGAAGACTATTTTGATATTACTAAAAAGTGGGTTAAAGTATT
ATTGTATACACAAAAAAAACAGGTTTTTTATATCACATTTTATGATATAACCAAATATAAAGTCAAAGAAATAGAATTGA
AAAATACAATTAAAAAATACAAATATCTTATAGAGTTATCTGCGGACGCAATAATAATTAGAAATTTAAATGGGGATATA
ATATATTGTAACAAATCTGCTCTTAATCTGTTTGGATATTCCTTGGAAGAAATGAAAAATCTAAATATGATAGACTTAGT
CCCTAGATCAGTTATGAAAGATATAGGGTTTAATATGTCCATAGGAGATAAGCCTGTTGAGAGAGTACATAAAAGAAAAG
ATGGAACACATTTTTATGGGGAGGAAACAACAAAATTAATAAATATAGAAGGGGAATTGGGAATTGCAACATATATAAGG
GATATAACAGAACGGAAAATATATAATGATAAAACGAAACAAATGGCTTATTTTGACTCTCTTACAGAGTTACCTAATAG
AAACTCTTTTTTAAAACAATTAGAGCATGAAATAAAATTAAGCAGGAAAAAACAAAACTTATTGGCTATAATGTTTCTAG
ATCTTGATAAATTTAAGGAAGTAAATGATAATTTTGGTCATTGCACTGGGGATAAACTTTTATGGCAAGTAGCTAAAAGG
GTAAAGAATACTATTAGTTCTAAAGATTTAATAGCTAGATTTGGAGGAGATGAATTTACTATATTAATAAGAAATATAGC
TCACGAAAAACAAGTTGAAGATTTAGCCAGAGATATAATAGAGGTCTTTAAAGAACCTATATGTATAGAAGGAATATATG
TAAATATAAAGACTAGTATAGGTATAAGCTTTTTTCCAAAAGATGGCCATACATCACAAGAACTAATAAAAAAAGCAGAT
AAAGCTATGTATGATGCTAAAAAAAGAGGGAGTAATAAATTTCAAATATATAAAGATTAA

Upstream 100 bases:

>100_bases
AGTTATTGGGGAAAATTCCTTAATTATAAATACTTATTTGAAGCTATAGGGAATATATTAATAGTATATCAATATATAAC
TGAACATAAGAGGTGAAACT

Downstream 100 bases:

>100_bases
AAAAGTATTGTGTAAAGCTTAAATATAATAATTTAATATTTTTAATAAAAATTAGGGATGTATTTTTATAGATAATAGGT
ATGTTATTTATATAAATATA

Product: putative sensory box-containing diguanylate cyclase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MKEKNLIKQMELSVIEKMGIAYCCCKISLNSYGQLEDFIFMESNDYFKDLTGFTKEYIKNKKAKDIIAFGSHRINIMKAL
EKILLYKEEKIEFEDYFDITKKWVKVLLYTQKKQVFYITFYDITKYKVKEIELKNTIKKYKYLIELSADAIIIRNLNGDI
IYCNKSALNLFGYSLEEMKNLNMIDLVPRSVMKDIGFNMSIGDKPVERVHKRKDGTHFYGEETTKLINIEGELGIATYIR
DITERKIYNDKTKQMAYFDSLTELPNRNSFLKQLEHEIKLSRKKQNLLAIMFLDLDKFKEVNDNFGHCTGDKLLWQVAKR
VKNTISSKDLIARFGGDEFTILIRNIAHEKQVEDLARDIIEVFKEPICIEGIYVNIKTSIGISFFPKDGHTSQELIKKAD
KAMYDAKKRGSNKFQIYKD

Sequences:

>Translated_419_residues
MKEKNLIKQMELSVIEKMGIAYCCCKISLNSYGQLEDFIFMESNDYFKDLTGFTKEYIKNKKAKDIIAFGSHRINIMKAL
EKILLYKEEKIEFEDYFDITKKWVKVLLYTQKKQVFYITFYDITKYKVKEIELKNTIKKYKYLIELSADAIIIRNLNGDI
IYCNKSALNLFGYSLEEMKNLNMIDLVPRSVMKDIGFNMSIGDKPVERVHKRKDGTHFYGEETTKLINIEGELGIATYIR
DITERKIYNDKTKQMAYFDSLTELPNRNSFLKQLEHEIKLSRKKQNLLAIMFLDLDKFKEVNDNFGHCTGDKLLWQVAKR
VKNTISSKDLIARFGGDEFTILIRNIAHEKQVEDLARDIIEVFKEPICIEGIYVNIKTSIGISFFPKDGHTSQELIKKAD
KAMYDAKKRGSNKFQIYKD
>Mature_419_residues
MKEKNLIKQMELSVIEKMGIAYCCCKISLNSYGQLEDFIFMESNDYFKDLTGFTKEYIKNKKAKDIIAFGSHRINIMKAL
EKILLYKEEKIEFEDYFDITKKWVKVLLYTQKKQVFYITFYDITKYKVKEIELKNTIKKYKYLIELSADAIIIRNLNGDI
IYCNKSALNLFGYSLEEMKNLNMIDLVPRSVMKDIGFNMSIGDKPVERVHKRKDGTHFYGEETTKLINIEGELGIATYIR
DITERKIYNDKTKQMAYFDSLTELPNRNSFLKQLEHEIKLSRKKQNLLAIMFLDLDKFKEVNDNFGHCTGDKLLWQVAKR
VKNTISSKDLIARFGGDEFTILIRNIAHEKQVEDLARDIIEVFKEPICIEGIYVNIKTSIGISFFPKDGHTSQELIKKAD
KAMYDAKKRGSNKFQIYKD

Specific function: Probable signaling protein whose physiological role is not yet known [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1787541, Length=177, Percent_Identity=36.1581920903955, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI87081881, Length=316, Percent_Identity=29.746835443038, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1788381, Length=282, Percent_Identity=27.6595744680851, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI145693134, Length=176, Percent_Identity=32.3863636363636, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787262, Length=164, Percent_Identity=32.3170731707317, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI87082007, Length=185, Percent_Identity=27.5675675675676, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786584, Length=169, Percent_Identity=27.810650887574, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787816, Length=181, Percent_Identity=29.2817679558011, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788956, Length=168, Percent_Identity=30.952380952381, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI87081977, Length=114, Percent_Identity=37.719298245614, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1787802, Length=181, Percent_Identity=30.3867403314917, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787056, Length=156, Percent_Identity=32.6923076923077, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788085, Length=168, Percent_Identity=29.1666666666667, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR005330
- InterPro:   IPR000014 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF03707 MHYT [H]

EC number: NA

Molecular weight: Translated: 49124; Mature: 49124

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: PS50112 PAS ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEKNLIKQMELSVIEKMGIAYCCCKISLNSYGQLEDFIFMESNDYFKDLTGFTKEYIKN
CCCHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHHCC
KKAKDIIAFGSHRINIMKALEKILLYKEEKIEFEDYFDITKKWVKVLLYTQKKQVFYITF
CCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEECCCEEEEEEE
YDITKYKVKEIELKNTIKKYKYLIELSADAIIIRNLNGDIIYCNKSALNLFGYSLEEMKN
EECCCHHHHHHHHHHHHHHHHHHEEECCCEEEEEECCCCEEEECCHHHHHHCCCHHHHCC
LNMIDLVPRSVMKDIGFNMSIGDKPVERVHKRKDGTHFYGEETTKLINIEGELGIATYIR
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEECCCCCEEEEEECCCCHHHHHHH
DITERKIYNDKTKQMAYFDSLTELPNRNSFLKQLEHEIKLSRKKQNLLAIMFLDLDKFKE
HHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCEEEEEEECHHHHHH
VNDNFGHCTGDKLLWQVAKRVKNTISSKDLIARFGGDEFTILIRNIAHEKQVEDLARDII
HCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHH
EVFKEPICIEGIYVNIKTSIGISFFPKDGHTSQELIKKADKAMYDAKKRGSNKFQIYKD
HHHCCCEEEEEEEEEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEC
>Mature Secondary Structure
MKEKNLIKQMELSVIEKMGIAYCCCKISLNSYGQLEDFIFMESNDYFKDLTGFTKEYIKN
CCCHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHHCC
KKAKDIIAFGSHRINIMKALEKILLYKEEKIEFEDYFDITKKWVKVLLYTQKKQVFYITF
CCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEECCCEEEEEEE
YDITKYKVKEIELKNTIKKYKYLIELSADAIIIRNLNGDIIYCNKSALNLFGYSLEEMKN
EECCCHHHHHHHHHHHHHHHHHHEEECCCEEEEEECCCCEEEECCHHHHHHCCCHHHHCC
LNMIDLVPRSVMKDIGFNMSIGDKPVERVHKRKDGTHFYGEETTKLINIEGELGIATYIR
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEECCCCCEEEEEECCCCHHHHHHH
DITERKIYNDKTKQMAYFDSLTELPNRNSFLKQLEHEIKLSRKKQNLLAIMFLDLDKFKE
HHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCEEEEEEECHHHHHH
VNDNFGHCTGDKLLWQVAKRVKNTISSKDLIARFGGDEFTILIRNIAHEKQVEDLARDII
HCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHH
EVFKEPICIEGIYVNIKTSIGISFFPKDGHTSQELIKKADKAMYDAKKRGSNKFQIYKD
HHHCCCEEEEEEEEEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 11728710 [H]