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The map label for this gene is prsA

Identifier: 170755263

GI number: 170755263

Start: 3861343

End: 3862302

Strand: Reverse

Name: prsA

Synonym: CLD_0939

Alternate gene names: 170755263

Gene position: 3862302-3861343 (Counterclockwise)

Preceding gene: 170754276

Following gene: 170755568

Centisome position: 97.58

GC content: 30.94

Gene sequence:

>960_bases
ATGATAACCCATGGAAAAGGTATTAAAATTTTTTCAGGTAATTCTCACCCGAAATTAGCTCAAGATATTGCTAGTATTTT
AGGTACTACTGTAGGTGATTCACAAGTAGGAACATTTAGTGATGGGGAAATATCTGTTAATATTAATGAAACAGTTAGGG
GAACAGATTTATTTATTGTACAATCAACTAATGAACCAGTTAATGATAACTTAATGGAACTTTTAATAATGATTGATGCA
TTTAAAAGAGCTTCAGCAGGAAGAATAACAGCAGTTGTTCCTTATTATGGATATGCAAGACAAGATAGAAAAGCTAAGGC
TAGGGATCCAATTACAGCTAAATTAGTTGCGGACCTTTTAACAGCAGCAGGAGCAGATAGAGTATTAACAATGGATTTAC
ATGCATCTCAAATTCAAGGATATTTTAACATTCCATTAGATCATTTATTAGGATCACCAATACTTGCAAAATATTTTGTA
CAAAAAGGATTTGCAGATAGAAATGATATAGTTGTAGTATCTCCAGATTTAGGAAGTGTAACTAGAGCTAGAAAATTTGC
TGATAAGCTACAATGCCCGATAGCTATTATAGATAAAAGAAGACCTAAAGCAAACGTATCAGAGGTAATGAATATAATAG
GAGATATAAAAGATAAGACTGTTATATTAGTTGATGATATGATAGATACAGCTGGAACTATAACTAATGGAGCTAATGCT
TTAATAGAAATGGGAGCTAAAGAAGTTTATGCTTGTTGCACTCATGCTGTTTTATCTGGACCAGCTATAGAAAGAATAGA
AAAGTCAGTAATAAAAGAATTAGTTATGTTAAACACTATAGATTTACCAGAAGAAAAAAATCTAGATAAATTTAAAGTTT
TATCAGTTGCGCCTGTATTTGCAGAAGCAATTAAGAGAATATACGAAGATACATCAGTAAGTAAAATATTTGAAGATTAA

Upstream 100 bases:

>100_bases
AAGGTTCTTTAGCAATTGCTAGATCAAAGCAGATCAATAAAGAAGGCTGGTTAGATAAAAAAGGATTATTAAAGAAATAA
AATTTAAGGAGGACTATATA

Downstream 100 bases:

>100_bases
TCAATACATATAAAATAAAGTACTCATAGAAAATAAATTCTATGGGTACTTTATTTATATAGTTAACTATTTGTAAATTT
TGAAGAATTATTTATTTTTA

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MITHGKGIKIFSGNSHPKLAQDIASILGTTVGDSQVGTFSDGEISVNINETVRGTDLFIVQSTNEPVNDNLMELLIMIDA
FKRASAGRITAVVPYYGYARQDRKAKARDPITAKLVADLLTAAGADRVLTMDLHASQIQGYFNIPLDHLLGSPILAKYFV
QKGFADRNDIVVVSPDLGSVTRARKFADKLQCPIAIIDKRRPKANVSEVMNIIGDIKDKTVILVDDMIDTAGTITNGANA
LIEMGAKEVYACCTHAVLSGPAIERIEKSVIKELVMLNTIDLPEEKNLDKFKVLSVAPVFAEAIKRIYEDTSVSKIFED

Sequences:

>Translated_319_residues
MITHGKGIKIFSGNSHPKLAQDIASILGTTVGDSQVGTFSDGEISVNINETVRGTDLFIVQSTNEPVNDNLMELLIMIDA
FKRASAGRITAVVPYYGYARQDRKAKARDPITAKLVADLLTAAGADRVLTMDLHASQIQGYFNIPLDHLLGSPILAKYFV
QKGFADRNDIVVVSPDLGSVTRARKFADKLQCPIAIIDKRRPKANVSEVMNIIGDIKDKTVILVDDMIDTAGTITNGANA
LIEMGAKEVYACCTHAVLSGPAIERIEKSVIKELVMLNTIDLPEEKNLDKFKVLSVAPVFAEAIKRIYEDTSVSKIFED
>Mature_319_residues
MITHGKGIKIFSGNSHPKLAQDIASILGTTVGDSQVGTFSDGEISVNINETVRGTDLFIVQSTNEPVNDNLMELLIMIDA
FKRASAGRITAVVPYYGYARQDRKAKARDPITAKLVADLLTAAGADRVLTMDLHASQIQGYFNIPLDHLLGSPILAKYFV
QKGFADRNDIVVVSPDLGSVTRARKFADKLQCPIAIIDKRRPKANVSEVMNIIGDIKDKTVILVDDMIDTAGTITNGANA
LIEMGAKEVYACCTHAVLSGPAIERIEKSVIKELVMLNTIDLPEEKNLDKFKVLSVAPVFAEAIKRIYEDTSVSKIFED

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506127, Length=313, Percent_Identity=49.2012779552716, Blast_Score=301, Evalue=7e-82,
Organism=Homo sapiens, GI4506129, Length=313, Percent_Identity=47.9233226837061, Blast_Score=297, Evalue=1e-80,
Organism=Homo sapiens, GI84875539, Length=316, Percent_Identity=47.4683544303797, Blast_Score=291, Evalue=4e-79,
Organism=Homo sapiens, GI28557709, Length=311, Percent_Identity=47.9099678456592, Blast_Score=291, Evalue=7e-79,
Organism=Homo sapiens, GI4506133, Length=353, Percent_Identity=35.6940509915014, Blast_Score=188, Evalue=7e-48,
Organism=Homo sapiens, GI194018537, Length=348, Percent_Identity=34.1954022988506, Blast_Score=176, Evalue=4e-44,
Organism=Homo sapiens, GI310128524, Length=144, Percent_Identity=32.6388888888889, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI310115209, Length=144, Percent_Identity=32.6388888888889, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI310118259, Length=144, Percent_Identity=32.6388888888889, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI310119946, Length=144, Percent_Identity=32.6388888888889, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI1787458, Length=314, Percent_Identity=55.7324840764331, Blast_Score=347, Evalue=6e-97,
Organism=Caenorhabditis elegans, GI25149168, Length=313, Percent_Identity=46.3258785942492, Blast_Score=288, Evalue=3e-78,
Organism=Caenorhabditis elegans, GI17554702, Length=313, Percent_Identity=46.3258785942492, Blast_Score=287, Evalue=4e-78,
Organism=Caenorhabditis elegans, GI71989924, Length=314, Percent_Identity=46.1783439490446, Blast_Score=286, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI17554704, Length=311, Percent_Identity=45.9807073954984, Blast_Score=285, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI17570245, Length=342, Percent_Identity=32.1637426900585, Blast_Score=193, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6320946, Length=312, Percent_Identity=46.1538461538462, Blast_Score=270, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6319403, Length=317, Percent_Identity=45.7413249211356, Blast_Score=270, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6321776, Length=315, Percent_Identity=45.3968253968254, Blast_Score=263, Evalue=4e-71,
Organism=Saccharomyces cerevisiae, GI6322667, Length=200, Percent_Identity=43, Blast_Score=169, Evalue=7e-43,
Organism=Saccharomyces cerevisiae, GI6324511, Length=112, Percent_Identity=41.0714285714286, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21355239, Length=315, Percent_Identity=48.8888888888889, Blast_Score=301, Evalue=5e-82,
Organism=Drosophila melanogaster, GI45551540, Length=338, Percent_Identity=45.5621301775148, Blast_Score=287, Evalue=5e-78,
Organism=Drosophila melanogaster, GI24651458, Length=361, Percent_Identity=32.409972299169, Blast_Score=191, Evalue=6e-49,
Organism=Drosophila melanogaster, GI24651456, Length=361, Percent_Identity=32.409972299169, Blast_Score=191, Evalue=6e-49,
Organism=Drosophila melanogaster, GI281362873, Length=361, Percent_Identity=32.409972299169, Blast_Score=191, Evalue=8e-49,
Organism=Drosophila melanogaster, GI24651454, Length=361, Percent_Identity=32.409972299169, Blast_Score=191, Evalue=8e-49,
Organism=Drosophila melanogaster, GI24651462, Length=207, Percent_Identity=37.6811594202899, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24651464, Length=207, Percent_Identity=37.6811594202899, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI45552010, Length=207, Percent_Identity=37.6811594202899, Blast_Score=145, Evalue=3e-35,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 34749; Mature: 34749

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00114 PRPP_SYNTHETASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITHGKGIKIFSGNSHPKLAQDIASILGTTVGDSQVGTFSDGEISVNINETVRGTDLFIV
CCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEE
QSTNEPVNDNLMELLIMIDAFKRASAGRITAVVPYYGYARQDRKAKARDPITAKLVADLL
ECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHH
TAAGADRVLTMDLHASQIQGYFNIPLDHLLGSPILAKYFVQKGFADRNDIVVVSPDLGSV
HHCCCCCEEEEECCHHHHCCEECCCHHHHHCCHHHHHHHHHHCCCCCCCEEEECCCCCHH
TRARKFADKLQCPIAIIDKRRPKANVSEVMNIIGDIKDKTVILVDDMIDTAGTITNGANA
HHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEECHHHCCCCCCCCHHH
LIEMGAKEVYACCTHAVLSGPAIERIEKSVIKELVMLNTIDLPEEKNLDKFKVLSVAPVF
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
AEAIKRIYEDTSVSKIFED
HHHHHHHHCCCHHHHHHCC
>Mature Secondary Structure
MITHGKGIKIFSGNSHPKLAQDIASILGTTVGDSQVGTFSDGEISVNINETVRGTDLFIV
CCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEE
QSTNEPVNDNLMELLIMIDAFKRASAGRITAVVPYYGYARQDRKAKARDPITAKLVADLL
ECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHH
TAAGADRVLTMDLHASQIQGYFNIPLDHLLGSPILAKYFVQKGFADRNDIVVVSPDLGSV
HHCCCCCEEEEECCHHHHCCEECCCHHHHHCCHHHHHHHHHHCCCCCCCEEEECCCCCHH
TRARKFADKLQCPIAIIDKRRPKANVSEVMNIIGDIKDKTVILVDDMIDTAGTITNGANA
HHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEECHHHCCCCCCCCHHH
LIEMGAKEVYACCTHAVLSGPAIERIEKSVIKELVMLNTIDLPEEKNLDKFKVLSVAPVF
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
AEAIKRIYEDTSVSKIFED
HHHHHHHHCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792842 [H]