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The map label for this gene is cobS

Identifier: 170755235

GI number: 170755235

Start: 933763

End: 934509

Strand: Direct

Name: cobS

Synonym: CLD_3751

Alternate gene names: 170755235

Gene position: 933763-934509 (Clockwise)

Preceding gene: 170754284

Following gene: 170754215

Centisome position: 23.59

GC content: 26.24

Gene sequence:

>747_bases
ATGAAGAGTATATTAAATGATTTTTTATTAATGATTCAATTTTTTACACGTATACCTGTAAATAAAAACTTACAGTGTGA
AAAAGAAAATTTTAGAAGGGGAGCTTTTTTTCTTCCAGTAGTTGCTTTCATTATAGGTGGAATGGAGTTTTTAATATATT
TAGCCCTTAAAAATTTTTTACCGCCTAATGTAATAATAGTGTTATTGATTTTATTTACAGCTATGATTACTGGAGGCCTT
CATATGGATGGACTAGCAGATACCTGTGATGGTTTTTTTTCTTTAAGAGATAAGGAAAGAATAATAGAAATTATGAAAGA
CAGTAGAATAGGGTCCTACGGAACCATAGCATTAATAATTGATTTGCTATTAAAATATCAACTCCTATATTCTTTAGTTT
TAAAAGGATACAGTATAGCTATAGTCTTAGCGCCTATCATAGGAAGAATTAGTATTTTATTTTTATGTTTATCTAAAAGA
ACAGCGAAAAAAAATGGTTCCGGAAATATATTTATAGGAAATATGAGTAAACCTATAATATTTTTTATTACCACAATAGT
ATTAGCGTTAAGTACATATTTTTTAGGATTGAGAGCTACAATAATTCCATTTATAGGAGTTTTGTTAATAACTTATTTAT
TATATTTATTATGTTTAAACAAAATAAATGGACTTACAGGTGATACCTTGGGAGCTTGTAATGAACTAGGTGAAATAACA
TTTTTATTAATTTTATTAATGATTTAA

Upstream 100 bases:

>100_bases
ATCCATTATTTATTTTTGGAAAACTATAATATGGAATTTAATAAATTTTTGTTTATACGAAATTTAGGTAATTAATAATA
AATATAAAAGAGGGGAAAAC

Downstream 100 bases:

>100_bases
AAACTTTAATAGAAGGTGTTCTAAAATAAATCTATTTTGAAATATTTTCATAAGAGTAAGAGGGATATTAAAAAAATTCT
TTTAGCTTTTAATGAGAGTA

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MKSILNDFLLMIQFFTRIPVNKNLQCEKENFRRGAFFLPVVAFIIGGMEFLIYLALKNFLPPNVIIVLLILFTAMITGGL
HMDGLADTCDGFFSLRDKERIIEIMKDSRIGSYGTIALIIDLLLKYQLLYSLVLKGYSIAIVLAPIIGRISILFLCLSKR
TAKKNGSGNIFIGNMSKPIIFFITTIVLALSTYFLGLRATIIPFIGVLLITYLLYLLCLNKINGLTGDTLGACNELGEIT
FLLILLMI

Sequences:

>Translated_248_residues
MKSILNDFLLMIQFFTRIPVNKNLQCEKENFRRGAFFLPVVAFIIGGMEFLIYLALKNFLPPNVIIVLLILFTAMITGGL
HMDGLADTCDGFFSLRDKERIIEIMKDSRIGSYGTIALIIDLLLKYQLLYSLVLKGYSIAIVLAPIIGRISILFLCLSKR
TAKKNGSGNIFIGNMSKPIIFFITTIVLALSTYFLGLRATIIPFIGVLLITYLLYLLCLNKINGLTGDTLGACNELGEIT
FLLILLMI
>Mature_248_residues
MKSILNDFLLMIQFFTRIPVNKNLQCEKENFRRGAFFLPVVAFIIGGMEFLIYLALKNFLPPNVIIVLLILFTAMITGGL
HMDGLADTCDGFFSLRDKERIIEIMKDSRIGSYGTIALIIDLLLKYQLLYSLVLKGYSIAIVLAPIIGRISILFLCLSKR
TAKKNGSGNIFIGNMSKPIIFFITTIVLALSTYFLGLRATIIPFIGVLLITYLLYLLCLNKINGLTGDTLGACNELGEIT
FLLILLMI

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=251, Percent_Identity=34.6613545816733, Blast_Score=148, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 27691; Mature: 27691

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSILNDFLLMIQFFTRIPVNKNLQCEKENFRRGAFFLPVVAFIIGGMEFLIYLALKNFL
CHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
PPNVIIVLLILFTAMITGGLHMDGLADTCDGFFSLRDKERIIEIMKDSRIGSYGTIALII
CHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
DLLLKYQLLYSLVLKGYSIAIVLAPIIGRISILFLCLSKRTAKKNGSGNIFIGNMSKPII
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHH
FFITTIVLALSTYFLGLRATIIPFIGVLLITYLLYLLCLNKINGLTGDTLGACNELGEIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
FLLILLMI
HHHHHHHC
>Mature Secondary Structure
MKSILNDFLLMIQFFTRIPVNKNLQCEKENFRRGAFFLPVVAFIIGGMEFLIYLALKNFL
CHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
PPNVIIVLLILFTAMITGGLHMDGLADTCDGFFSLRDKERIIEIMKDSRIGSYGTIALII
CHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
DLLLKYQLLYSLVLKGYSIAIVLAPIIGRISILFLCLSKRTAKKNGSGNIFIGNMSKPII
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHH
FFITTIVLALSTYFLGLRATIIPFIGVLLITYLLYLLCLNKINGLTGDTLGACNELGEIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
FLLILLMI
HHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA