| Definition | Clostridium botulinum B1 str. Okra, complete genome. |
|---|---|
| Accession | NC_010516 |
| Length | 3,958,233 |
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The map label for this gene is mrdA [H]
Identifier: 170755210
GI number: 170755210
Start: 3250007
End: 3252781
Strand: Reverse
Name: mrdA [H]
Synonym: CLD_1545
Alternate gene names: 170755210
Gene position: 3252781-3250007 (Counterclockwise)
Preceding gene: 170757079
Following gene: 170754502
Centisome position: 82.18
GC content: 31.24
Gene sequence:
>2775_bases ATGAAGATAAACAAAGATAAAAAATTTACTAGATTTGGTGGTCTTATGTTGGTAATGGTATTTATAATTACCGCAATTAT ATCTAGACTTGTTTATTTGCAACTTATGAATTCACAGGAGTACAAAGAAAAAGCAAATAATAGATCCATAAGAGAAATTC CAGATCCTGCTCCAAGAGGGAACATAATAGACAAAAATGGAGTAGTATTAGCCACTAATAAGCAAAATTACATGCTTATA TATAATGAAACAGCAGAAAATAAAGAAAGTTTTTTTTCTACTATGGAAAAAGTATTTAGCATATTAGATCAATATAAAGA AAAACAAACGGATGATCTTGAGCTTAAAATTAATCCTTATAGATTTGAATTTAAAGCTAGTACAGCTGAGGCAAAAAGGG CAGCAGAGTTAAGATTTAAAAAAGATAGAGGATTAGATGATGTAGTTACAAAAAAATTATTTTCAGGTAAAAATAAAAAA GAAAAAGAAGAATTAACAAAAGAAGATAAAGCTAAAATAGATGAGGAGTTATTAAAAATAACTCCAGAAGAAACCTTTAA ATATTTATTAGAACAATATAAAATAGATACAAAGAAATATTCTCTAGAACAGCAAAGAAGATTTATGATAGTAAAAGATG CAGCAAAAATGCAAAGTTTTTCAGGATATAAATCTGTTGATGTGGCAAGTAATATAAAGAAAGAAACAGCTTTTATATTC CTTCAGAAATTAAATGATTTACCAGGTATAGATGTAAGCACTCAACCCATAAGAGTTTATCCTCATAAGGAAGTAGGATC AGCAGTACTAGGCTATATATCTAAAATAAGTGGTGATAATGAGAAATATAAAGAAAAGGGATATGACCCAAGCTCTGATT ATATAGGTATAGATGGAATAGAGGGTGTTTTTGAAGAAAGACTTAAGGGATCTAAAGGTGGAAGAATTGTTAAACTTAAT AAAACAGGAAGGGTTATAGAAGAATTAGGAAGAAGAGAACCTTATCCAGGTCAAAATATACAACTTACTATAGATACGAA TATACAAAAGGCTGCAGAAGATTCGCTGGATTCTGTAATGAAAGATCTTCAACAAAGAGGAGTACAAGGCGATGTTAATA CATCTAATGCTACAAGAGGTGCTGCAGTAGCATTAGATGTAAATACAGGAGAAATTTTAGCTTTGGCAAGTAGGCCAGGC TTTGATCCTAACTTATTTGCAACTCCAGGAGGATTAACCCCAGAACTATATAAACAATATTTTAACCCGGATTTAACTGA GTTTGGAAAACAATATATACTTAATAAAGGATTAATGGGCTATTATCCGGGAAATACTTTAGACGAGGTTTTAGAAAAGC TATTTCCTTTAGATAAAAGTATAAAAAATAATAAAACTATGAGGCAAGATTTATATGATATATATCCAAAGCCTTTTTAT AATTATGCTACTATGTCTGTAGTACCTCCAGGATCTACATTTAAAGCTATGACAGCTATTGCAGGATTAGAATCAGGGGT TATAACTCCTGGATATTCTATAAGCGATACTGGCGTTTTTGATGACGGAAAGAAGTTTGTAAAGAAATTCGCAGTAGGTG GCTATGGTTCCGTTGATTTATATAGAGGATTAGAGGTTTCTAGTAACCCTTATTTTATGACAGTAGGTAAATTATTAAGA GAGAGTTTTGGTGATGATATACTTGCAAAATACGCTTGGAAATTTGGGTTAGGTGTTCCACCAAATTCAGATGAAAAAGC TTCTACAGGAATAGAAATTCCTGAAAAGTTTGGTCAAGTATTTAATACTTATACATTATCTAATGTTTATGCAACTCAAT ACTTGTGGCAAACTATGTCTACTTTAAAATCAGGAACAGATGCTAGGGGAAATAAATTTCCATCTATAAATTTATATGAT AGCGAAAAAGATTCAGACAAGGTTAAGGAATTAAAGAAACAAATTAAAAATTCTATACAGGACTCTATAAAAGAAGGAAC TAAAAAGTTTGATTCTAATAATTATATAAAAATGCTTACAGAGTTAGTTAATCAAGATCCTATCTATAAAGGTAAAAATG TAAGTAAAGATCAAATAAAAAGAATTGTAGATGTTATATACTATGTAACAATATCAGATGCTCACTTCCAGTTAGGTGTA GGTGCTAATATGTACAATGCTGCTATAGGACAAGGTATAAGTAACTTTACTCCATTACAATTGGCTAATTTTGTTAGTAC TATAGCTAATGGAGGAGATAGATATAAATTGCATTTGGTGAAAGAAATAAAAGATGCAGATGGTAATGTAGTAGAAACAG TTAAACCAGAGATAGTTGAAAAGACTAATGTAAGCAAAGAGAATTTAGCTGCTGTAAGGCAAGGAATGGCACGAGTTAAT GAGGGAACAGATGGTACTGCAGCAGCAGTGTTTAAGGATTTCCCTATACAAACAGCAGGTAAGACAGGATCTTCTACTTT TAGTAATAGTCAAGATTATTATGGAAGAACATCTTATGGTGTTTATATAGGATTTGCTCCAGTTGATAATCCAAAAATAG CAGTATGTGTTATTGTATTTGATGGTGGACATGGAGGATCTGTTGCACCTGTAGCAAAGGCTATGTATGAAGCCTATTTT AAGAAGGAACTTAATAAAGACAAACCAGATGCAGCTAAAGCATCAAATTCAGGGCAAAAGGAAAATACTAAGAATTCAGA TGCTAGCGGAGATGATGCAAAGGACATAGAAAATAAAAATACAAATAACAGATAA
Upstream 100 bases:
>100_bases GGGGTAATAACAATTATAGTTTATAAATTAGTATATAATTTATGTAGAAAAAAATATATGGATAAAAAGTGGGAATTCTA AAGGAATGGTGATTTAGTCA
Downstream 100 bases:
>100_bases ATAAATATAAACTATATCCACACTATAAATTATTTTATATAATAGATAGTGTGGATATTTTAAAATGGAAAATATTAAAT AAATAAAAGTATAATAAACA
Product: penicillin-binding protein
Products: NA
Alternate protein names: PBP-2 [H]
Number of amino acids: Translated: 924; Mature: 924
Protein sequence:
>924_residues MKINKDKKFTRFGGLMLVMVFIITAIISRLVYLQLMNSQEYKEKANNRSIREIPDPAPRGNIIDKNGVVLATNKQNYMLI YNETAENKESFFSTMEKVFSILDQYKEKQTDDLELKINPYRFEFKASTAEAKRAAELRFKKDRGLDDVVTKKLFSGKNKK EKEELTKEDKAKIDEELLKITPEETFKYLLEQYKIDTKKYSLEQQRRFMIVKDAAKMQSFSGYKSVDVASNIKKETAFIF LQKLNDLPGIDVSTQPIRVYPHKEVGSAVLGYISKISGDNEKYKEKGYDPSSDYIGIDGIEGVFEERLKGSKGGRIVKLN KTGRVIEELGRREPYPGQNIQLTIDTNIQKAAEDSLDSVMKDLQQRGVQGDVNTSNATRGAAVALDVNTGEILALASRPG FDPNLFATPGGLTPELYKQYFNPDLTEFGKQYILNKGLMGYYPGNTLDEVLEKLFPLDKSIKNNKTMRQDLYDIYPKPFY NYATMSVVPPGSTFKAMTAIAGLESGVITPGYSISDTGVFDDGKKFVKKFAVGGYGSVDLYRGLEVSSNPYFMTVGKLLR ESFGDDILAKYAWKFGLGVPPNSDEKASTGIEIPEKFGQVFNTYTLSNVYATQYLWQTMSTLKSGTDARGNKFPSINLYD SEKDSDKVKELKKQIKNSIQDSIKEGTKKFDSNNYIKMLTELVNQDPIYKGKNVSKDQIKRIVDVIYYVTISDAHFQLGV GANMYNAAIGQGISNFTPLQLANFVSTIANGGDRYKLHLVKEIKDADGNVVETVKPEIVEKTNVSKENLAAVRQGMARVN EGTDGTAAAVFKDFPIQTAGKTGSSTFSNSQDYYGRTSYGVYIGFAPVDNPKIAVCVIVFDGGHGGSVAPVAKAMYEAYF KKELNKDKPDAAKASNSGQKENTKNSDASGDDAKDIENKNTNNR
Sequences:
>Translated_924_residues MKINKDKKFTRFGGLMLVMVFIITAIISRLVYLQLMNSQEYKEKANNRSIREIPDPAPRGNIIDKNGVVLATNKQNYMLI YNETAENKESFFSTMEKVFSILDQYKEKQTDDLELKINPYRFEFKASTAEAKRAAELRFKKDRGLDDVVTKKLFSGKNKK EKEELTKEDKAKIDEELLKITPEETFKYLLEQYKIDTKKYSLEQQRRFMIVKDAAKMQSFSGYKSVDVASNIKKETAFIF LQKLNDLPGIDVSTQPIRVYPHKEVGSAVLGYISKISGDNEKYKEKGYDPSSDYIGIDGIEGVFEERLKGSKGGRIVKLN KTGRVIEELGRREPYPGQNIQLTIDTNIQKAAEDSLDSVMKDLQQRGVQGDVNTSNATRGAAVALDVNTGEILALASRPG FDPNLFATPGGLTPELYKQYFNPDLTEFGKQYILNKGLMGYYPGNTLDEVLEKLFPLDKSIKNNKTMRQDLYDIYPKPFY NYATMSVVPPGSTFKAMTAIAGLESGVITPGYSISDTGVFDDGKKFVKKFAVGGYGSVDLYRGLEVSSNPYFMTVGKLLR ESFGDDILAKYAWKFGLGVPPNSDEKASTGIEIPEKFGQVFNTYTLSNVYATQYLWQTMSTLKSGTDARGNKFPSINLYD SEKDSDKVKELKKQIKNSIQDSIKEGTKKFDSNNYIKMLTELVNQDPIYKGKNVSKDQIKRIVDVIYYVTISDAHFQLGV GANMYNAAIGQGISNFTPLQLANFVSTIANGGDRYKLHLVKEIKDADGNVVETVKPEIVEKTNVSKENLAAVRQGMARVN EGTDGTAAAVFKDFPIQTAGKTGSSTFSNSQDYYGRTSYGVYIGFAPVDNPKIAVCVIVFDGGHGGSVAPVAKAMYEAYF KKELNKDKPDAAKASNSGQKENTKNSDASGDDAKDIENKNTNNR >Mature_924_residues MKINKDKKFTRFGGLMLVMVFIITAIISRLVYLQLMNSQEYKEKANNRSIREIPDPAPRGNIIDKNGVVLATNKQNYMLI YNETAENKESFFSTMEKVFSILDQYKEKQTDDLELKINPYRFEFKASTAEAKRAAELRFKKDRGLDDVVTKKLFSGKNKK EKEELTKEDKAKIDEELLKITPEETFKYLLEQYKIDTKKYSLEQQRRFMIVKDAAKMQSFSGYKSVDVASNIKKETAFIF LQKLNDLPGIDVSTQPIRVYPHKEVGSAVLGYISKISGDNEKYKEKGYDPSSDYIGIDGIEGVFEERLKGSKGGRIVKLN KTGRVIEELGRREPYPGQNIQLTIDTNIQKAAEDSLDSVMKDLQQRGVQGDVNTSNATRGAAVALDVNTGEILALASRPG FDPNLFATPGGLTPELYKQYFNPDLTEFGKQYILNKGLMGYYPGNTLDEVLEKLFPLDKSIKNNKTMRQDLYDIYPKPFY NYATMSVVPPGSTFKAMTAIAGLESGVITPGYSISDTGVFDDGKKFVKKFAVGGYGSVDLYRGLEVSSNPYFMTVGKLLR ESFGDDILAKYAWKFGLGVPPNSDEKASTGIEIPEKFGQVFNTYTLSNVYATQYLWQTMSTLKSGTDARGNKFPSINLYD SEKDSDKVKELKKQIKNSIQDSIKEGTKKFDSNNYIKMLTELVNQDPIYKGKNVSKDQIKRIVDVIYYVTISDAHFQLGV GANMYNAAIGQGISNFTPLQLANFVSTIANGGDRYKLHLVKEIKDADGNVVETVKPEIVEKTNVSKENLAAVRQGMARVN EGTDGTAAAVFKDFPIQTAGKTGSSTFSNSQDYYGRTSYGVYIGFAPVDNPKIAVCVIVFDGGHGGSVAPVAKAMYEAYF KKELNKDKPDAAKASNSGQKENTKNSDASGDDAKDIENKNTNNR
Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]
COG id: COG0768
COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2
Gene ontology:
Cell location: Cell inner membrane [H]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the transpeptidase family [H]
Homologues:
Organism=Escherichia coli, GI1786854, Length=392, Percent_Identity=26.7857142857143, Blast_Score=107, Evalue=4e-24, Organism=Escherichia coli, GI1786272, Length=171, Percent_Identity=32.7485380116959, Blast_Score=82, Evalue=2e-16,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012338 - InterPro: IPR005311 - InterPro: IPR001460 - InterPro: IPR017790 [H]
Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]
EC number: NA
Molecular weight: Translated: 103057; Mature: 103057
Theoretical pI: Translated: 9.24; Mature: 9.24
Prosite motif: PS00216 SUGAR_TRANSPORT_1
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.1 %Cys (Translated Protein) 2.1 %Met (Translated Protein) 2.2 %Cys+Met (Translated Protein) 0.1 %Cys (Mature Protein) 2.1 %Met (Mature Protein) 2.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKINKDKKFTRFGGLMLVMVFIITAIISRLVYLQLMNSQEYKEKANNRSIREIPDPAPRG CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCC NIIDKNGVVLATNKQNYMLIYNETAENKESFFSTMEKVFSILDQYKEKQTDDLELKINPY CEECCCCEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCE RFEFKASTAEAKRAAELRFKKDRGLDDVVTKKLFSGKNKKEKEELTKEDKAKIDEELLKI EEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHC TPEETFKYLLEQYKIDTKKYSLEQQRRFMIVKDAAKMQSFSGYKSVDVASNIKKETAFIF CHHHHHHHHHHHHHCCHHHHHHHHHHCEEEEECHHHHHHCCCCCCHHHHHHHHHHHHHHH LQKLNDLPGIDVSTQPIRVYPHKEVGSAVLGYISKISGDNEKYKEKGYDPSSDYIGIDGI HHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCEECCCCH EGVFEERLKGSKGGRIVKLNKTGRVIEELGRREPYPGQNIQLTIDTNIQKAAEDSLDSVM HHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHH KDLQQRGVQGDVNTSNATRGAAVALDVNTGEILALASRPGFDPNLFATPGGLTPELYKQY HHHHHCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCEECCCCCCHHHHHHH FNPDLTEFGKQYILNKGLMGYYPGNTLDEVLEKLFPLDKSIKNNKTMRQDLYDIYPKPFY CCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCHHHHCCHHHHHHHHHHCCCCCC NYATMSVVPPGSTFKAMTAIAGLESGVITPGYSISDTGVFDDGKKFVKKFAVGGYGSVDL CCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHH YRGLEVSSNPYFMTVGKLLRESFGDDILAKYAWKFGLGVPPNSDEKASTGIEIPEKFGQV HCCCCCCCCCEEEHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHH FNTYTLSNVYATQYLWQTMSTLKSGTDARGNKFPSINLYDSEKDSDKVKELKKQIKNSIQ HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHH DSIKEGTKKFDSNNYIKMLTELVNQDPIYKGKNVSKDQIKRIVDVIYYVTISDAHFQLGV HHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHEEECCCEEEEEC GANMYNAAIGQGISNFTPLQLANFVSTIANGGDRYKLHLVKEIKDADGNVVETVKPEIVE CCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEHHHHCCCCCEEECCCHHHHH KTNVSKENLAAVRQGMARVNEGTDGTAAAVFKDFPIQTAGKTGSSTFSNSQDYYGRTSYG HCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCE VYIGFAPVDNPKIAVCVIVFDGGHGGSVAPVAKAMYEAYFKKELNKDKPDAAKASNSGQK EEEEEECCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCC ENTKNSDASGDDAKDIENKNTNNR CCCCCCCCCCCCHHHHCCCCCCCC >Mature Secondary Structure MKINKDKKFTRFGGLMLVMVFIITAIISRLVYLQLMNSQEYKEKANNRSIREIPDPAPRG CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCC NIIDKNGVVLATNKQNYMLIYNETAENKESFFSTMEKVFSILDQYKEKQTDDLELKINPY CEECCCCEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCE RFEFKASTAEAKRAAELRFKKDRGLDDVVTKKLFSGKNKKEKEELTKEDKAKIDEELLKI EEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHC TPEETFKYLLEQYKIDTKKYSLEQQRRFMIVKDAAKMQSFSGYKSVDVASNIKKETAFIF CHHHHHHHHHHHHHCCHHHHHHHHHHCEEEEECHHHHHHCCCCCCHHHHHHHHHHHHHHH LQKLNDLPGIDVSTQPIRVYPHKEVGSAVLGYISKISGDNEKYKEKGYDPSSDYIGIDGI HHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCEECCCCH EGVFEERLKGSKGGRIVKLNKTGRVIEELGRREPYPGQNIQLTIDTNIQKAAEDSLDSVM HHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHH KDLQQRGVQGDVNTSNATRGAAVALDVNTGEILALASRPGFDPNLFATPGGLTPELYKQY HHHHHCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCEECCCCCCHHHHHHH FNPDLTEFGKQYILNKGLMGYYPGNTLDEVLEKLFPLDKSIKNNKTMRQDLYDIYPKPFY CCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCHHHHCCHHHHHHHHHHCCCCCC NYATMSVVPPGSTFKAMTAIAGLESGVITPGYSISDTGVFDDGKKFVKKFAVGGYGSVDL CCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHH YRGLEVSSNPYFMTVGKLLRESFGDDILAKYAWKFGLGVPPNSDEKASTGIEIPEKFGQV HCCCCCCCCCEEEHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHH FNTYTLSNVYATQYLWQTMSTLKSGTDARGNKFPSINLYDSEKDSDKVKELKKQIKNSIQ HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHH DSIKEGTKKFDSNNYIKMLTELVNQDPIYKGKNVSKDQIKRIVDVIYYVTISDAHFQLGV HHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHEEECCCEEEEEC GANMYNAAIGQGISNFTPLQLANFVSTIANGGDRYKLHLVKEIKDADGNVVETVKPEIVE CCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEHHHHCCCCCEEECCCHHHHH KTNVSKENLAAVRQGMARVNEGTDGTAAAVFKDFPIQTAGKTGSSTFSNSQDYYGRTSYG HCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCE VYIGFAPVDNPKIAVCVIVFDGGHGGSVAPVAKAMYEAYFKKELNKDKPDAAKASNSGQK EEEEEECCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCC ENTKNSDASGDDAKDIENKNTNNR CCCCCCCCCCCCHHHHCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 7542800 [H]