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The map label for this gene is ksgA

Identifier: 170755195

GI number: 170755195

Start: 111623

End: 112450

Strand: Direct

Name: ksgA

Synonym: CLD_0706

Alternate gene names: 170755195

Gene position: 111623-112450 (Clockwise)

Preceding gene: 170755088

Following gene: 170757375

Centisome position: 2.82

GC content: 26.93

Gene sequence:

>828_bases
ATGAATACAAAGGAAATAGTTAACAAATATGAATTTAAGTTTAATAAGAATTTAGGACAAAACTTTTTAATAGATGAATC
TGTATTGGAAGATATAATAGAGGGAGCTGAAATAAGCAAAGAAGATACTGTTATAGAAATAGGTCCCGGAGTTGGAACTT
TAACTAAAGAGCTTTTAGAAAGAGCAAAAGAAGTTTACTCTATAGAATTAGATGGTGATTTAATACCTATTTTGCAAGAG
GAGTTAAAGGAATATAATAATTTTACATTAATACATAAAGATGCGTTAAAAATTAATTTTAATGAGCTAATGGAAAATAA
GGATTCCATAAAATTAGTAGCCAATCTACCTTATTATGTAACTACACCTATTATATCAAGATTACTTACAGAAAAATGTG
ATTTTAAATCACTAACCATAATGATTCAAAAGGAAGTAGCGGAAAGAATAAATGCTGAACCTAACTGTAAGGAGTATGGT
TCATTAACCGTTTTAGTACAATATTATTGTAATACAAAAATAATAAGAAAAGTATCTCCAAATTCTTTTATACCAAAACC
TAAAGTGGATTCTATAGTTATAAAATTAGATAGACTTTCAGAACCAAGAGTAAGAGTGAAAAGCCAAAAATTATTTTTTA
ATGTAGTAAGAAGTTCATTTAATATGAGAAGAAAAACTTTATGGAATTCATTAAAAAGTTTAAATATAGATAAAGAAAGT
ATGGAAAATGCTTTTGAAAGAGCAGGGATAGATCCAAAACGAAGGGGCGAGACTTTATCCATAGAGGAATTCGGAAAACT
TTCAGATTGTATATATGATATATTATAA

Upstream 100 bases:

>100_bases
TGCAATACTAATCAGTTTATAAAAAGACTTAACAATTTTGGTATAACCAAAGAAGAATTTATAAAGGCTATAAAAGATTT
AGATAAGGGGAATGAATAAC

Downstream 100 bases:

>100_bases
TTTAAATAATTAAGTTCACTTTTTGTAAGATAACTCATATATTATATTGAATAATTATATTTGGGGGTACAATAAAAAGT
GGCACAAAAAAAGAATTATA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MNTKEIVNKYEFKFNKNLGQNFLIDESVLEDIIEGAEISKEDTVIEIGPGVGTLTKELLERAKEVYSIELDGDLIPILQE
ELKEYNNFTLIHKDALKINFNELMENKDSIKLVANLPYYVTTPIISRLLTEKCDFKSLTIMIQKEVAERINAEPNCKEYG
SLTVLVQYYCNTKIIRKVSPNSFIPKPKVDSIVIKLDRLSEPRVRVKSQKLFFNVVRSSFNMRRKTLWNSLKSLNIDKES
MENAFERAGIDPKRRGETLSIEEFGKLSDCIYDIL

Sequences:

>Translated_275_residues
MNTKEIVNKYEFKFNKNLGQNFLIDESVLEDIIEGAEISKEDTVIEIGPGVGTLTKELLERAKEVYSIELDGDLIPILQE
ELKEYNNFTLIHKDALKINFNELMENKDSIKLVANLPYYVTTPIISRLLTEKCDFKSLTIMIQKEVAERINAEPNCKEYG
SLTVLVQYYCNTKIIRKVSPNSFIPKPKVDSIVIKLDRLSEPRVRVKSQKLFFNVVRSSFNMRRKTLWNSLKSLNIDKES
MENAFERAGIDPKRRGETLSIEEFGKLSDCIYDIL
>Mature_275_residues
MNTKEIVNKYEFKFNKNLGQNFLIDESVLEDIIEGAEISKEDTVIEIGPGVGTLTKELLERAKEVYSIELDGDLIPILQE
ELKEYNNFTLIHKDALKINFNELMENKDSIKLVANLPYYVTTPIISRLLTEKCDFKSLTIMIQKEVAERINAEPNCKEYG
SLTVLVQYYCNTKIIRKVSPNSFIPKPKVDSIVIKLDRLSEPRVRVKSQKLFFNVVRSSFNMRRKTLWNSLKSLNIDKES
MENAFERAGIDPKRRGETLSIEEFGKLSDCIYDIL

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI156415992, Length=288, Percent_Identity=30.9027777777778, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI7657198, Length=223, Percent_Identity=32.2869955156951, Blast_Score=110, Evalue=1e-24,
Organism=Escherichia coli, GI1786236, Length=257, Percent_Identity=36.1867704280156, Blast_Score=170, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI25141369, Length=293, Percent_Identity=26.6211604095563, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI25146882, Length=197, Percent_Identity=34.010152284264, Blast_Score=111, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6324989, Length=227, Percent_Identity=31.7180616740088, Blast_Score=112, Evalue=6e-26,
Organism=Drosophila melanogaster, GI21358017, Length=201, Percent_Identity=34.3283582089552, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI21357273, Length=284, Percent_Identity=29.2253521126761, Blast_Score=112, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 31757; Mature: 31757

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTKEIVNKYEFKFNKNLGQNFLIDESVLEDIIEGAEISKEDTVIEIGPGVGTLTKELLE
CCHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHH
RAKEVYSIELDGDLIPILQEELKEYNNFTLIHKDALKINFNELMENKDSIKLVANLPYYV
HHHHHEEEEECCCCHHHHHHHHHHHCCEEEEECCCEEEEHHHHHCCCCCEEEEECCCHHH
TTPIISRLLTEKCDFKSLTIMIQKEVAERINAEPNCKEYGSLTVLVQYYCNTKIIRKVSP
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHCCEEEEEEHHHCCCEEEECCC
NSFIPKPKVDSIVIKLDRLSEPRVRVKSQKLFFNVVRSSFNMRRKTLWNSLKSLNIDKES
CCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
MENAFERAGIDPKRRGETLSIEEFGKLSDCIYDIL
HHHHHHHCCCCHHHCCCEECHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNTKEIVNKYEFKFNKNLGQNFLIDESVLEDIIEGAEISKEDTVIEIGPGVGTLTKELLE
CCHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHH
RAKEVYSIELDGDLIPILQEELKEYNNFTLIHKDALKINFNELMENKDSIKLVANLPYYV
HHHHHEEEEECCCCHHHHHHHHHHHCCEEEEECCCEEEEHHHHHCCCCCEEEEECCCHHH
TTPIISRLLTEKCDFKSLTIMIQKEVAERINAEPNCKEYGSLTVLVQYYCNTKIIRKVSP
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHCCEEEEEEHHHCCCEEEECCC
NSFIPKPKVDSIVIKLDRLSEPRVRVKSQKLFFNVVRSSFNMRRKTLWNSLKSLNIDKES
CCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
MENAFERAGIDPKRRGETLSIEEFGKLSDCIYDIL
HHHHHHHCCCCHHHCCCEECHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA