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The map label for this gene is ogt [H]

Identifier: 170755012

GI number: 170755012

Start: 758803

End: 759255

Strand: Reverse

Name: ogt [H]

Synonym: CLD_0122

Alternate gene names: 170755012

Gene position: 759255-758803 (Counterclockwise)

Preceding gene: 170754344

Following gene: 170757659

Centisome position: 19.18

GC content: 42.38

Gene sequence:

>453_bases
ATGATAAGGTACGCATTTTACGATTTTGAATTTGGAACTCTAAAAATAGGATATACCGATGAAAGAGTCGTCTTCTTGAA
GCGGGTGGAACAAATCAACGCAGATAATGAACCTTCTGAGCTTTCCGATCTTGCCTTTGGACAGGTGCGTGAATATCTCA
AAGGAAATCGTCATGACTTTGACTTTCCATACGAGCTTGATGGTACGGATTTTCAGAAAAAAGTGTGGAATGCACTTTGC
CGGATACCCTATGGAGAGACTCGCACCTACAAACAGATTGCCATGGTGGTCGGAAATTCGAAAGCGAGTCGTGCAGTTGG
CATGGCAAACAACAAAAATCCTTTGACAATTGTCGTTCCGTGCCATCGAGTAGTTGGAACAAACGGAAATCTTATTGGCT
ATGCTGGTGGTTTGAATATGAAAAAAGCTCTCCTAGAGCTTGAACGGAAGTAA

Upstream 100 bases:

>100_bases
TTGCTTACTCTGTAGGATTCTCCGGTCTATCATCCTTTTACCGCTTTTTCAAAACTGGTACCGGATTGTCTCCTGCCGCT
TATAGAAAGGAGCATCGTCA

Downstream 100 bases:

>100_bases
AACACAATAGATGAACGGAATATATTTCCAAATACAAATTAAAATGTTGGTAATTAAAGCGAAAAGTACCTTGCAGGCAC
TTCTCGCTTTTAAATTCTTT

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 150; Mature: 150

Protein sequence:

>150_residues
MIRYAFYDFEFGTLKIGYTDERVVFLKRVEQINADNEPSELSDLAFGQVREYLKGNRHDFDFPYELDGTDFQKKVWNALC
RIPYGETRTYKQIAMVVGNSKASRAVGMANNKNPLTIVVPCHRVVGTNGNLIGYAGGLNMKKALLELERK

Sequences:

>Translated_150_residues
MIRYAFYDFEFGTLKIGYTDERVVFLKRVEQINADNEPSELSDLAFGQVREYLKGNRHDFDFPYELDGTDFQKKVWNALC
RIPYGETRTYKQIAMVVGNSKASRAVGMANNKNPLTIVVPCHRVVGTNGNLIGYAGGLNMKKALLELERK
>Mature_150_residues
MIRYAFYDFEFGTLKIGYTDERVVFLKRVEQINADNEPSELSDLAFGQVREYLKGNRHDFDFPYELDGTDFQKKVWNALC
RIPYGETRTYKQIAMVVGNSKASRAVGMANNKNPLTIVVPCHRVVGTNGNLIGYAGGLNMKKALLELERK

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=80, Percent_Identity=45, Blast_Score=74, Evalue=6e-14,
Organism=Escherichia coli, GI1787596, Length=120, Percent_Identity=48.3333333333333, Blast_Score=108, Evalue=1e-25,
Organism=Escherichia coli, GI1788542, Length=163, Percent_Identity=38.6503067484663, Blast_Score=105, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI115533070, Length=80, Percent_Identity=48.75, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI115533068, Length=80, Percent_Identity=48.75, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320001, Length=89, Percent_Identity=46.0674157303371, Blast_Score=84, Evalue=6e-18,
Organism=Drosophila melanogaster, GI17137554, Length=88, Percent_Identity=46.5909090909091, Blast_Score=66, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 17091; Mature: 17091

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRYAFYDFEFGTLKIGYTDERVVFLKRVEQINADNEPSELSDLAFGQVREYLKGNRHDF
CEEEEEEECCCCEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
DFPYELDGTDFQKKVWNALCRIPYGETRTYKQIAMVVGNSKASRAVGMANNKNPLTIVVP
CCCEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEECCCCCCCCEEEEEE
CHRVVGTNGNLIGYAGGLNMKKALLELERK
CHHEECCCCCEEEEECCCCHHHHHHHHCCC
>Mature Secondary Structure
MIRYAFYDFEFGTLKIGYTDERVVFLKRVEQINADNEPSELSDLAFGQVREYLKGNRHDF
CEEEEEEECCCCEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
DFPYELDGTDFQKKVWNALCRIPYGETRTYKQIAMVVGNSKASRAVGMANNKNPLTIVVP
CCCEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEECCCCCCCCEEEEEE
CHRVVGTNGNLIGYAGGLNMKKALLELERK
CHHEECCCCCEEEEECCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]