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The map label for this gene is truB

Identifier: 170754990

GI number: 170754990

Start: 2534500

End: 2535369

Strand: Reverse

Name: truB

Synonym: CLD_2225

Alternate gene names: 170754990

Gene position: 2535369-2534500 (Counterclockwise)

Preceding gene: 170756269

Following gene: 170754240

Centisome position: 64.05

GC content: 28.16

Gene sequence:

>870_bases
ATGGATGGAGTAATAAATGTTTTAAAACCAAAGGGAATAACATCCTTTGATGTAGTTAGAGATATAAGAAAAATAGCGAA
AATAAAAAAAGTTGGTCATACAGGTACATTAGATCCCTTAGCTTCTGGTGTTCTCCCAGTATGTATAGGTAAAGCTACAA
AAATAGTAGACTATATAATGGAGGGAACCAAGACCTATAGAGTAGAAATGAAACTTGGCACTACTACGGATACCTATGAT
AGAGAAGGTACTGTGTTAGAGGAAAAAGAAGTTAGTGTATCTTCTACAGAAGTAGAAAAGGCTATAGAAAAATATAAAGG
TGATATAGAACAAGTGCCACCTATGTATTCTGCCTTGAAAATTAATGGACAAAAACTTTATGATTTAGCTAGAAAAGGTA
TAGAGGTAGAAAGAGAAGCAAGAAAAATACATATATATGATATATGTATTTTAAGTATAGATCTACCTTATATTATATTT
GATGTTAAGTGTTCAAAGGGGACTTATATAAGGAGTTTGTGTTTTGATATAGGAAATGATTTAGGCTCAGGAGCAGTAAT
GTGGAATCTGCAAAGATTAGAAGCAAGCCCCTTTAATATAAAAGAAGCAATAAAATTAGAAGATTTAAATGAGAAAAATA
TAAAACAGTATATAACACCTATAGATGAAGCTTTAAATGACTATGAAAAATTATATTTAGATAAAAAATTTGAAAAACTT
GTACTAAATGGTGTAATATTAAAAGATAAAAGAGTTTTAGATAATATAGAAGAAAACAAGCTATATAGAACTTACATAGA
AAATGATAATTTTATAGGATTAGGAATGAAAAATGCATATGGATTTAAAATTCATAAATTATTAACTTAG

Upstream 100 bases:

>100_bases
TGGAGGCCATACTCGTGCCTCAGGAGCCTTTATAAAGGGAGAAGCTTTAAATATAGTAGAAAAAAAATTAATAGAAACTA
TAAAAAAAGAGTTGATATAA

Downstream 100 bases:

>100_bases
GAGTAATAGCTTATGATAGTTATGGAGGATAACTTTTCTACTAAATTAGAAGATAAAACTTATATAGCTTTAGGAAGTTT
TGATGGACTTCATAAAGGTC

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MDGVINVLKPKGITSFDVVRDIRKIAKIKKVGHTGTLDPLASGVLPVCIGKATKIVDYIMEGTKTYRVEMKLGTTTDTYD
REGTVLEEKEVSVSSTEVEKAIEKYKGDIEQVPPMYSALKINGQKLYDLARKGIEVEREARKIHIYDICILSIDLPYIIF
DVKCSKGTYIRSLCFDIGNDLGSGAVMWNLQRLEASPFNIKEAIKLEDLNEKNIKQYITPIDEALNDYEKLYLDKKFEKL
VLNGVILKDKRVLDNIEENKLYRTYIENDNFIGLGMKNAYGFKIHKLLT

Sequences:

>Translated_289_residues
MDGVINVLKPKGITSFDVVRDIRKIAKIKKVGHTGTLDPLASGVLPVCIGKATKIVDYIMEGTKTYRVEMKLGTTTDTYD
REGTVLEEKEVSVSSTEVEKAIEKYKGDIEQVPPMYSALKINGQKLYDLARKGIEVEREARKIHIYDICILSIDLPYIIF
DVKCSKGTYIRSLCFDIGNDLGSGAVMWNLQRLEASPFNIKEAIKLEDLNEKNIKQYITPIDEALNDYEKLYLDKKFEKL
VLNGVILKDKRVLDNIEENKLYRTYIENDNFIGLGMKNAYGFKIHKLLT
>Mature_289_residues
MDGVINVLKPKGITSFDVVRDIRKIAKIKKVGHTGTLDPLASGVLPVCIGKATKIVDYIMEGTKTYRVEMKLGTTTDTYD
REGTVLEEKEVSVSSTEVEKAIEKYKGDIEQVPPMYSALKINGQKLYDLARKGIEVEREARKIHIYDICILSIDLPYIIF
DVKCSKGTYIRSLCFDIGNDLGSGAVMWNLQRLEASPFNIKEAIKLEDLNEKNIKQYITPIDEALNDYEKLYLDKKFEKL
VLNGVILKDKRVLDNIEENKLYRTYIENDNFIGLGMKNAYGFKIHKLLT

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=226, Percent_Identity=38.0530973451327, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI4503337, Length=279, Percent_Identity=27.5985663082437, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI215599015, Length=279, Percent_Identity=27.5985663082437, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI2367200, Length=288, Percent_Identity=37.5, Blast_Score=203, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17553978, Length=192, Percent_Identity=31.7708333333333, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324037, Length=140, Percent_Identity=40, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6323204, Length=243, Percent_Identity=27.5720164609054, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281364189, Length=195, Percent_Identity=32.8205128205128, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI281364187, Length=195, Percent_Identity=32.8205128205128, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI281364185, Length=195, Percent_Identity=32.8205128205128, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI281364183, Length=195, Percent_Identity=32.8205128205128, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI62471759, Length=195, Percent_Identity=32.8205128205128, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI17975520, Length=195, Percent_Identity=32.8205128205128, Blast_Score=95, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 32872; Mature: 32872

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGVINVLKPKGITSFDVVRDIRKIAKIKKVGHTGTLDPLASGVLPVCIGKATKIVDYIM
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHH
EGTKTYRVEMKLGTTTDTYDREGTVLEEKEVSVSSTEVEKAIEKYKGDIEQVPPMYSALK
CCCEEEEEEEEECCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCHHHCCCCHHHEE
INGQKLYDLARKGIEVEREARKIHIYDICILSIDLPYIIFDVKCSKGTYIRSLCFDIGND
ECCHHHHHHHHCCCCEECCCCEEEEEEEEEEEECCCEEEEEEEECCCHHHHHHHHHHCCC
LGSGAVMWNLQRLEASPFNIKEAIKLEDLNEKNIKQYITPIDEALNDYEKLYLDKKFEKL
CCCCEEEEEEHHHCCCCCCHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLNGVILKDKRVLDNIEENKLYRTYIENDNFIGLGMKNAYGFKIHKLLT
HHCCEEEECHHHHCCHHHCCEEEEEECCCCEEEEECCCCCCEEEEEECC
>Mature Secondary Structure
MDGVINVLKPKGITSFDVVRDIRKIAKIKKVGHTGTLDPLASGVLPVCIGKATKIVDYIM
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHH
EGTKTYRVEMKLGTTTDTYDREGTVLEEKEVSVSSTEVEKAIEKYKGDIEQVPPMYSALK
CCCEEEEEEEEECCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCHHHCCCCHHHEE
INGQKLYDLARKGIEVEREARKIHIYDICILSIDLPYIIFDVKCSKGTYIRSLCFDIGND
ECCHHHHHHHHCCCCEECCCCEEEEEEEEEEEECCCEEEEEEEECCCHHHHHHHHHHCCC
LGSGAVMWNLQRLEASPFNIKEAIKLEDLNEKNIKQYITPIDEALNDYEKLYLDKKFEKL
CCCCEEEEEEHHHCCCCCCHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLNGVILKDKRVLDNIEENKLYRTYIENDNFIGLGMKNAYGFKIHKLLT
HHCCEEEECHHHHCCHHHCCEEEEEECCCCEEEEECCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA