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The map label for this gene is flp [H]

Identifier: 170754966

GI number: 170754966

Start: 784716

End: 785426

Strand: Direct

Name: flp [H]

Synonym: CLD_0100

Alternate gene names: 170754966

Gene position: 784716-785426 (Clockwise)

Preceding gene: 170757413

Following gene: 170757213

Centisome position: 19.82

GC content: 27.29

Gene sequence:

>711_bases
ATGAAAGAGTGTACTTGTGGAAGATGTCATCACAATATTTGTGCAAGGAAAGTTCCTATATTTTCTTCTTTATCCGAAGA
AGAGTTAATAAAAATTGTAAATATGACGGGACATGAGGCTTTTAAAAAGGGAGAAGCCATTTGCAATGAAGGAGATAAAT
CAGAAACTTTAATTATTATAAATGAAGGGAAAGTAAAACTTTGTAAGCTTACTAAAGAAGGAAAAGAACAAATTATTCAT
ATACTTTCCAGTGGAGATTTTTTTGGAGAACTAAATCTTTTTAATGATAATAAAAGCTATAATTTTTCCGCTTATGCTAT
ATCCGATGTAAAAATATGTACCCTTACAAAAAATAAGATGGACGAGATATTATTAAAAAATCCAGATATATCTTTAAAGA
TATTAAAAGAAGTTACTAAAAGACTTGCAGAAACGGAAACTTTAGCTCAAAATTTAGCTACCAATGATGCAGATATTAGA
ATAGCTAATATGATATTAGATTTTGGTGAAAAATATGGAGTAAATAAAGAACAATATATAGAAATAAAACTGCCTATAAA
TAGGGAAGAGATGGCAAACTATGTAGGGGTTACAAGAGAAACTATAAGTAGAAAATTATCTAAATTCGAGGATTTAGATA
TTATAAAACTTGTTGGTAATAAGGTAATTATAATTAAAGATGAAGATGCATTAAAAGAATTTATAGAATAG

Upstream 100 bases:

>100_bases
TATCTTATAATTCTATAGATTATAACAATATAGGCTATATGTTATATCTGCCATAAAGGTAATTTGAATAATAATTATAA
TAACAAAGGGGGTGTTTTTT

Downstream 100 bases:

>100_bases
ATTATTTTAGAGAAAAGTGCTTTGCACTTTTTTATTTTTTTGAAAATTTGATTTGAATCATATTTTTAAATTCCAATAGT
TTATATAATGAAGCCATAAC

Product: Crp/FNR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MKECTCGRCHHNICARKVPIFSSLSEEELIKIVNMTGHEAFKKGEAICNEGDKSETLIIINEGKVKLCKLTKEGKEQIIH
ILSSGDFFGELNLFNDNKSYNFSAYAISDVKICTLTKNKMDEILLKNPDISLKILKEVTKRLAETETLAQNLATNDADIR
IANMILDFGEKYGVNKEQYIEIKLPINREEMANYVGVTRETISRKLSKFEDLDIIKLVGNKVIIIKDEDALKEFIE

Sequences:

>Translated_236_residues
MKECTCGRCHHNICARKVPIFSSLSEEELIKIVNMTGHEAFKKGEAICNEGDKSETLIIINEGKVKLCKLTKEGKEQIIH
ILSSGDFFGELNLFNDNKSYNFSAYAISDVKICTLTKNKMDEILLKNPDISLKILKEVTKRLAETETLAQNLATNDADIR
IANMILDFGEKYGVNKEQYIEIKLPINREEMANYVGVTRETISRKLSKFEDLDIIKLVGNKVIIIKDEDALKEFIE
>Mature_236_residues
MKECTCGRCHHNICARKVPIFSSLSEEELIKIVNMTGHEAFKKGEAICNEGDKSETLIIINEGKVKLCKLTKEGKEQIIH
ILSSGDFFGELNLFNDNKSYNFSAYAISDVKICTLTKNKMDEILLKNPDISLKILKEVTKRLAETETLAQNLATNDADIR
IANMILDFGEKYGVNKEQYIEIKLPINREEMANYVGVTRETISRKLSKFEDLDIIKLVGNKVIIIKDEDALKEFIE

Specific function: This Protein Complexes With Cyclic AMP And Binds To Specific DNA Sites Near The Promoter To Regulate The Transcription Of Several Catabolite-Sensitive Operons. The Protein Induces A Severe Bend In The DNA. Acts As A Negative Regulator Of Its Own Synthesi

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI4758958, Length=114, Percent_Identity=35.0877192982456, Blast_Score=64, Evalue=1e-10,
Organism=Escherichia coli, GI1789756, Length=185, Percent_Identity=29.7297297297297, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI1787595, Length=213, Percent_Identity=25.3521126760563, Blast_Score=81, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 26794; Mature: 26794

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKECTCGRCHHNICARKVPIFSSLSEEELIKIVNMTGHEAFKKGEAICNEGDKSETLIII
CCCCCCCHHHHHHHHHCCCHHHCCCHHHHHHHHCCCCHHHHHHCHHHHCCCCCCCEEEEE
NEGKVKLCKLTKEGKEQIIHILSSGDFFGELNLFNDNKSYNFSAYAISDVKICTLTKNKM
ECCCEEEEECCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEEEEECCEEEEEECCCHH
DEILLKNPDISLKILKEVTKRLAETETLAQNLATNDADIRIANMILDFGEKYGVNKEQYI
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHCCCCHHEE
EIKLPINREEMANYVGVTRETISRKLSKFEDLDIIKLVGNKVIIIKDEDALKEFIE
EEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECHHHHHHHCC
>Mature Secondary Structure
MKECTCGRCHHNICARKVPIFSSLSEEELIKIVNMTGHEAFKKGEAICNEGDKSETLIII
CCCCCCCHHHHHHHHHCCCHHHCCCHHHHHHHHCCCCHHHHHHCHHHHCCCCCCCEEEEE
NEGKVKLCKLTKEGKEQIIHILSSGDFFGELNLFNDNKSYNFSAYAISDVKICTLTKNKM
ECCCEEEEECCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEEEEECCEEEEEECCCHH
DEILLKNPDISLKILKEVTKRLAETETLAQNLATNDADIRIANMILDFGEKYGVNKEQYI
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHCCCCHHEE
EIKLPINREEMANYVGVTRETISRKLSKFEDLDIIKLVGNKVIIIKDEDALKEFIE
EEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2113923; 8441692 [H]