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The map label for this gene is ygaF [C]

Identifier: 170754904

GI number: 170754904

Start: 2441843

End: 2443291

Strand: Reverse

Name: ygaF [C]

Synonym: CLD_2331

Alternate gene names: 170754904

Gene position: 2443291-2441843 (Counterclockwise)

Preceding gene: 170756057

Following gene: 170756021

Centisome position: 61.73

GC content: 32.16

Gene sequence:

>1449_bases
ATGTATGATGTTAGTATTATAGGTGCTGGAGTTGTTGGATCAGCCATTGCTAGAGAGTTATCAAAGTATAATTTAAAAGT
ATGTCTTATAGAAAAAGAGGAGGATGTTGGAACAGGAGCTTCCAAAGCAAATAGTGGTATTGTTCATGGTGGATATGTAG
CTAAATATGGAACTCTAAAGGGAGAATTATGTATAAGAGGAAACAGTATGTACAATAAGCTGGAAAAAGAGCTTAACTTT
GGATATAGAAATCCAGGTGCATTAGTAATAGGCTTTGATGAAGATGATGAAAATAGAATAAAAAAATTATATGAAAATGG
TATCAAAGTAGGATGCAATGATTTGGAGATAATATACGGGGATAAAATAAAAGAATTGGAGCCTCATATAAATAAAGATG
TTAAAGTTGCATTATATGCTAAAAGTGTTGGTGTAGCTTCTCCTTATGAAATGACTATAGCCCTTGCCGAAAATGCAATA
GAAAATGGAATAGATTTAAAATTAGAAACAAAAGTATTAGCTATAGATAAAGAACATGAGGCTTTTATAATAAATACTAA
TAAAGGAGAAATAAAAAGTAAGTATATTGTAAATGCTGCAGGATTATATAGTGATAAGATAGCCAATATGCTAGAGATGG
ATGATTTTAAAATATTGCCAAGGAGAGGGCAGTATGTACTTTCTACAAAAGATCAGGGTTATCTTGTAAATAAGGTTATA
TTCCAAGTACCAACAGAAAAAGGTAAAGGCATATTAGTTACTACTACTTATCATGGAAATTTTATGATAGGGCCTGATGC
ACAGGAGGTAGTAGACAAAGAAGACATAGGAACGGATATAGAAAGTATAGAATATATAATAAAAACAGCTAGAAAATCTA
TACCAGATTTTGATGTAAGAAGATCTTTAACTACCTTTGCAGGAATAAGAGCAATTAGTAGTACAGGAGATTTTGTAATA
GGAGAAACTAAAGTTAAAGGCTTTATAAATGCAGCAGGTATAGATTCACCAGGACTTACATCTTCACCAGCTATAGCAGA
AAAAATAGTAGGAATCCTTAAGGAATCTGGATTGCAATTAATAAAGAATGAAAAATTCAATCCCTACAGAAAGCCTATAA
TTATTAAAAAAGATAAGTCCTTTGATGGAAAGATAGATCATGAAGATCCAACTAAAAACATAATATGTAGGTGTGAAAAG
GTAACGGAATCAGAAATAATAGATGCAATGAAAAGAGGAATTCCTGTAAAATCTACAGATGCAATAAAAAGACGTACAAG
GGCAGGTATGGGGTTTTGCCAAGGTAATTTCTGTAGACCAAGAGTAAAAGCTATAATAGCAAGAGAAATAGGCATACCTG
CAGAACAGGTTACTGTAAGAGGCAAAGAGGGAGGAGAGCCTCCTAAAAGAGTAAATATTAATGTAATAAGAAAAATTCAA
GGTGTTTGA

Upstream 100 bases:

>100_bases
TTATTTTAGGATGTAAAATAGTATTAAGAAAATAATAGAAAATATTAAAAGATATATATTATTAATAAGCTTGAAATAAT
AAGAATAAGAGGGTGAGACT

Downstream 100 bases:

>100_bases
TTTCTTTTAAATATTAATAAAATATAAGGAAATGCCTAAGATGTAAATAAGGGCTGATAATAAAAAAGTAGCTCTTATTT
ACATTTTAGGTATAACTTTA

Product: oxidoreductase, FAD/[2Fe-2S]-binding

Products: glycerone phosphate; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MYDVSIIGAGVVGSAIARELSKYNLKVCLIEKEEDVGTGASKANSGIVHGGYVAKYGTLKGELCIRGNSMYNKLEKELNF
GYRNPGALVIGFDEDDENRIKKLYENGIKVGCNDLEIIYGDKIKELEPHINKDVKVALYAKSVGVASPYEMTIALAENAI
ENGIDLKLETKVLAIDKEHEAFIINTNKGEIKSKYIVNAAGLYSDKIANMLEMDDFKILPRRGQYVLSTKDQGYLVNKVI
FQVPTEKGKGILVTTTYHGNFMIGPDAQEVVDKEDIGTDIESIEYIIKTARKSIPDFDVRRSLTTFAGIRAISSTGDFVI
GETKVKGFINAAGIDSPGLTSSPAIAEKIVGILKESGLQLIKNEKFNPYRKPIIIKKDKSFDGKIDHEDPTKNIICRCEK
VTESEIIDAMKRGIPVKSTDAIKRRTRAGMGFCQGNFCRPRVKAIIAREIGIPAEQVTVRGKEGGEPPKRVNINVIRKIQ
GV

Sequences:

>Translated_482_residues
MYDVSIIGAGVVGSAIARELSKYNLKVCLIEKEEDVGTGASKANSGIVHGGYVAKYGTLKGELCIRGNSMYNKLEKELNF
GYRNPGALVIGFDEDDENRIKKLYENGIKVGCNDLEIIYGDKIKELEPHINKDVKVALYAKSVGVASPYEMTIALAENAI
ENGIDLKLETKVLAIDKEHEAFIINTNKGEIKSKYIVNAAGLYSDKIANMLEMDDFKILPRRGQYVLSTKDQGYLVNKVI
FQVPTEKGKGILVTTTYHGNFMIGPDAQEVVDKEDIGTDIESIEYIIKTARKSIPDFDVRRSLTTFAGIRAISSTGDFVI
GETKVKGFINAAGIDSPGLTSSPAIAEKIVGILKESGLQLIKNEKFNPYRKPIIIKKDKSFDGKIDHEDPTKNIICRCEK
VTESEIIDAMKRGIPVKSTDAIKRRTRAGMGFCQGNFCRPRVKAIIAREIGIPAEQVTVRGKEGGEPPKRVNINVIRKIQ
GV
>Mature_482_residues
MYDVSIIGAGVVGSAIARELSKYNLKVCLIEKEEDVGTGASKANSGIVHGGYVAKYGTLKGELCIRGNSMYNKLEKELNF
GYRNPGALVIGFDEDDENRIKKLYENGIKVGCNDLEIIYGDKIKELEPHINKDVKVALYAKSVGVASPYEMTIALAENAI
ENGIDLKLETKVLAIDKEHEAFIINTNKGEIKSKYIVNAAGLYSDKIANMLEMDDFKILPRRGQYVLSTKDQGYLVNKVI
FQVPTEKGKGILVTTTYHGNFMIGPDAQEVVDKEDIGTDIESIEYIIKTARKSIPDFDVRRSLTTFAGIRAISSTGDFVI
GETKVKGFINAAGIDSPGLTSSPAIAEKIVGILKESGLQLIKNEKFNPYRKPIIIKKDKSFDGKIDHEDPTKNIICRCEK
VTESEIIDAMKRGIPVKSTDAIKRRTRAGMGFCQGNFCRPRVKAIIAREIGIPAEQVTVRGKEGGEPPKRVNINVIRKIQ
GV

Specific function: Unknown

COG id: COG0579

COG function: function code R; Predicted dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI13376331, Length=278, Percent_Identity=28.0575539568345, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI24797151, Length=237, Percent_Identity=28.2700421940928, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI194306651, Length=359, Percent_Identity=21.7270194986072, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI87082148, Length=257, Percent_Identity=24.9027237354086, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788574, Length=482, Percent_Identity=22.6141078838174, Blast_Score=84, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17565764, Length=402, Percent_Identity=29.3532338308458, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24585081, Length=407, Percent_Identity=28.7469287469287, Blast_Score=111, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.1.99.5

Molecular weight: Translated: 53056; Mature: 53056

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDVSIIGAGVVGSAIARELSKYNLKVCLIEKEEDVGTGASKANSGIVHGGYVAKYGTLK
CEEEEEECCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCEEECCEEEEECCCC
GELCIRGNSMYNKLEKELNFGYRNPGALVIGFDEDDENRIKKLYENGIKVGCNDLEIIYG
CEEEEECCHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHCCCEECCCCEEEEEC
DKIKELEPHINKDVKVALYAKSVGVASPYEMTIALAENAIENGIDLKLETKVLAIDKEHE
CCHHHCCCCCCCCEEEEEEEECCCCCCCHHEEEEEHHHHHHCCCCEEEEEEEEEEECCCC
AFIINTNKGEIKSKYIVNAAGLYSDKIANMLEMDDFKILPRRGQYVLSTKDQGYLVNKVI
EEEEECCCCCCCCEEEEEECCCCHHHHHHHHHCCCEEECCCCCCEEEECCCCCEEEEEEE
FQVPTEKGKGILVTTTYHGNFMIGPDAQEVVDKEDIGTDIESIEYIIKTARKSIPDFDVR
EEECCCCCCEEEEEEEECCCEEECCCHHHHHCHHHCCCCHHHHHHHHHHHHHCCCCHHHH
RSLTTFAGIRAISSTGDFVIGETKVKGFINAAGIDSPGLTSSPAIAEKIVGILKESGLQL
HHHHHHHHHHEECCCCCEEEECHHHCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCHHH
IKNEKFNPYRKPIIIKKDKSFDGKIDHEDPTKNIICRCEKVTESEIIDAMKRGIPVKSTD
HHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHEEEHHCCHHHHHHHHHHCCCCCCHH
AIKRRTRAGMGFCQGNFCRPRVKAIIAREIGIPAEQVTVRGKEGGEPPKRVNINVIRKIQ
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHEEEECCCCCCCCCEECCEEEEEEC
GV
CC
>Mature Secondary Structure
MYDVSIIGAGVVGSAIARELSKYNLKVCLIEKEEDVGTGASKANSGIVHGGYVAKYGTLK
CEEEEEECCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCEEECCEEEEECCCC
GELCIRGNSMYNKLEKELNFGYRNPGALVIGFDEDDENRIKKLYENGIKVGCNDLEIIYG
CEEEEECCHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHCCCEECCCCEEEEEC
DKIKELEPHINKDVKVALYAKSVGVASPYEMTIALAENAIENGIDLKLETKVLAIDKEHE
CCHHHCCCCCCCCEEEEEEEECCCCCCCHHEEEEEHHHHHHCCCCEEEEEEEEEEECCCC
AFIINTNKGEIKSKYIVNAAGLYSDKIANMLEMDDFKILPRRGQYVLSTKDQGYLVNKVI
EEEEECCCCCCCCEEEEEECCCCHHHHHHHHHCCCEEECCCCCCEEEECCCCCEEEEEEE
FQVPTEKGKGILVTTTYHGNFMIGPDAQEVVDKEDIGTDIESIEYIIKTARKSIPDFDVR
EEECCCCCCEEEEEEEECCCEEECCCHHHHHCHHHCCCCHHHHHHHHHHHHHCCCCHHHH
RSLTTFAGIRAISSTGDFVIGETKVKGFINAAGIDSPGLTSSPAIAEKIVGILKESGLQL
HHHHHHHHHHEECCCCCEEEECHHHCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCHHH
IKNEKFNPYRKPIIIKKDKSFDGKIDHEDPTKNIICRCEKVTESEIIDAMKRGIPVKSTD
HHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHEEEHHCCHHHHHHHHHHCCCCCCHH
AIKRRTRAGMGFCQGNFCRPRVKAIIAREIGIPAEQVTVRGKEGGEPPKRVNINVIRKIQ
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHEEEECCCCCCCCCEECCEEEEEEC
GV
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: sn-glycerol 3-phosphate; acceptor

Specific reaction: sn-glycerol 3-phosphate + acceptor = glycerone phosphate + reduced acceptor

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7569993 [H]