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The map label for this gene is ascB [H]

Identifier: 170754806

GI number: 170754806

Start: 992825

End: 994246

Strand: Direct

Name: ascB [H]

Synonym: CLD_3684

Alternate gene names: 170754806

Gene position: 992825-994246 (Clockwise)

Preceding gene: 170757673

Following gene: 170754869

Centisome position: 25.08

GC content: 32.14

Gene sequence:

>1422_bases
ATGAGTGGATTAGCAAAGAATTTTTTATGGGGTGGAGCTACAGCAGCTAACCAATGCGAAGGTGGTTATTTAGAAGGAAA
TAGAGGATTAGGAACTGTGGACGTTATTCCAGCAGGAGTAAATCGTAGAGCTGTTATGCAAGGAAAGATGCATTATAATA
ATTTACAAGAGGATTCTTATTTCCCATCACATGAAGCAATAGATATGTATCATAACTATAAGGAAGATATAGCATTATTT
GCAGAAATGGGCTTTAAATGTTATCGTTTCTCATTTTCATGGTCAAGAATTTTCCCAACAGGAAAGGAACAAGAACCAAA
TGAAGAAGGCTTAGCATTTTATGAAAAATTTATTGATGAACTATTAAAATATAATATTGAACCAGTAGTTACTATATGTC
ACTTTGATGTACCTCTTGCATTGGAGCAGGAATATGGTTCATGGAGAAGTAGAAAATTAATAGATGCATATCTTAGATAT
TGTGAAGCAATATTTAAAAGATTTAATGGGAAAGTAAAGTATTGGATGACTTTTAATGAAATAAATATGCTTATGCATTT
ACCCTTTATGGGAGCTGGAATAATGTTTGAGGAAGGGGAAAATGAGTTAGAAGTAAAATATCAAGCAGCTCATAATGAAT
TAGTGGCATCAGCTTTAGCTACAAAATTAGCAAAGGAAATTAATAAAGATAATAAAATAGGATGCATGTTAGCGGCAGGG
CAGTTCTATCCATATACTTGTAAGCCAGAGGATGTTCTAGAGGCTATGGAGAAAGATAAAAATAATTATTTCTTCATAGA
TGTACAATCTAGGGGAGAATACCCGAGCTATGCTAAAAAATTCTTAGAGAGAAACAATATAAAAATAGAAATGCAGTCAG
AAGATGAGAAGATTTTAAGGGAGAATACTGTTGATTTTATTGCATTCAGTTACTATACTTCAAGACTTGCAAGTAGTGAC
CCCAATGCAGGAGAAAAATCAGCTGGTAATGTAATAACATCATTAAAAAATCCACATTTAAAATCTAGTAAATGGGGATG
GCAAATAGATCCAATAGGCCTTAGAATTACAATGAATTCACTTTATGATCGTTATCAAAAACCGCTATTCATTGTGGAAA
ATGGCTTAGGTGCTGTTGATGTTTTAAATGAAGATGGGACTGTAGATGATGATTATAGAATTGCTTATCTTGAAACTCAT
ATAAAAAATATGATTGATGCAGTTGAATTAGATGGTGTTGAACTATTAGGATATACAACTTGGGGATGCATTGATTTAGT
TAGTGCATCAACAGGGGAAATGAAAAAGCGATATGGTTTTATATATGTAGATAAGGATAATGATGGTCATGGAACTTTAA
AGAGATACAAGAAAAAATCTTTCTATTGGTACAAAAATGTTATTAAAAATAATGGACTATAA

Upstream 100 bases:

>100_bases
TAGCAAAATGAAGTGTTTATAAATGTATTATTTATATTTACAGTTTTAAAATTAAAGCTATTTTGAGACAACACCTTATT
TAATGAAAGGAAGGTAAAAT

Downstream 100 bases:

>100_bases
ATAATTGAAAAAATATATAATATATAAACTTGTAAAAGCAATAGATCCAAAAAATAAGGGAAGATTAGTAATGTAAAATA
TTTTAGGTTTTACTGAGGCA

Product: glycosy hydrolase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MSGLAKNFLWGGATAANQCEGGYLEGNRGLGTVDVIPAGVNRRAVMQGKMHYNNLQEDSYFPSHEAIDMYHNYKEDIALF
AEMGFKCYRFSFSWSRIFPTGKEQEPNEEGLAFYEKFIDELLKYNIEPVVTICHFDVPLALEQEYGSWRSRKLIDAYLRY
CEAIFKRFNGKVKYWMTFNEINMLMHLPFMGAGIMFEEGENELEVKYQAAHNELVASALATKLAKEINKDNKIGCMLAAG
QFYPYTCKPEDVLEAMEKDKNNYFFIDVQSRGEYPSYAKKFLERNNIKIEMQSEDEKILRENTVDFIAFSYYTSRLASSD
PNAGEKSAGNVITSLKNPHLKSSKWGWQIDPIGLRITMNSLYDRYQKPLFIVENGLGAVDVLNEDGTVDDDYRIAYLETH
IKNMIDAVELDGVELLGYTTWGCIDLVSASTGEMKKRYGFIYVDKDNDGHGTLKRYKKKSFYWYKNVIKNNGL

Sequences:

>Translated_473_residues
MSGLAKNFLWGGATAANQCEGGYLEGNRGLGTVDVIPAGVNRRAVMQGKMHYNNLQEDSYFPSHEAIDMYHNYKEDIALF
AEMGFKCYRFSFSWSRIFPTGKEQEPNEEGLAFYEKFIDELLKYNIEPVVTICHFDVPLALEQEYGSWRSRKLIDAYLRY
CEAIFKRFNGKVKYWMTFNEINMLMHLPFMGAGIMFEEGENELEVKYQAAHNELVASALATKLAKEINKDNKIGCMLAAG
QFYPYTCKPEDVLEAMEKDKNNYFFIDVQSRGEYPSYAKKFLERNNIKIEMQSEDEKILRENTVDFIAFSYYTSRLASSD
PNAGEKSAGNVITSLKNPHLKSSKWGWQIDPIGLRITMNSLYDRYQKPLFIVENGLGAVDVLNEDGTVDDDYRIAYLETH
IKNMIDAVELDGVELLGYTTWGCIDLVSASTGEMKKRYGFIYVDKDNDGHGTLKRYKKKSFYWYKNVIKNNGL
>Mature_472_residues
SGLAKNFLWGGATAANQCEGGYLEGNRGLGTVDVIPAGVNRRAVMQGKMHYNNLQEDSYFPSHEAIDMYHNYKEDIALFA
EMGFKCYRFSFSWSRIFPTGKEQEPNEEGLAFYEKFIDELLKYNIEPVVTICHFDVPLALEQEYGSWRSRKLIDAYLRYC
EAIFKRFNGKVKYWMTFNEINMLMHLPFMGAGIMFEEGENELEVKYQAAHNELVASALATKLAKEINKDNKIGCMLAAGQ
FYPYTCKPEDVLEAMEKDKNNYFFIDVQSRGEYPSYAKKFLERNNIKIEMQSEDEKILRENTVDFIAFSYYTSRLASSDP
NAGEKSAGNVITSLKNPHLKSSKWGWQIDPIGLRITMNSLYDRYQKPLFIVENGLGAVDVLNEDGTVDDDYRIAYLETHI
KNMIDAVELDGVELLGYTTWGCIDLVSASTGEMKKRYGFIYVDKDNDGHGTLKRYKKKSFYWYKNVIKNNGL

Specific function: Can hydrolyze salicin, cellobiose, and probably arbutin [H]

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI32481206, Length=493, Percent_Identity=28.3975659229209, Blast_Score=181, Evalue=2e-45,
Organism=Homo sapiens, GI13273313, Length=490, Percent_Identity=28.3673469387755, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI110681710, Length=492, Percent_Identity=28.8617886178862, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI24497614, Length=486, Percent_Identity=27.7777777777778, Blast_Score=150, Evalue=4e-36,
Organism=Homo sapiens, GI28376633, Length=484, Percent_Identity=26.0330578512397, Blast_Score=149, Evalue=4e-36,
Organism=Escherichia coli, GI1789070, Length=472, Percent_Identity=60.3813559322034, Blast_Score=622, Evalue=1e-179,
Organism=Escherichia coli, GI2367174, Length=480, Percent_Identity=51.875, Blast_Score=515, Evalue=1e-147,
Organism=Escherichia coli, GI2367270, Length=473, Percent_Identity=51.7970401691332, Blast_Score=512, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI17552856, Length=489, Percent_Identity=30.2658486707566, Blast_Score=171, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI17539390, Length=493, Percent_Identity=28.3975659229209, Blast_Score=157, Evalue=9e-39,
Organism=Drosophila melanogaster, GI21356577, Length=470, Percent_Identity=25.3191489361702, Blast_Score=137, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 54364; Mature: 54233

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1 ; PS00653 GLYCOSYL_HYDROL_F1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGLAKNFLWGGATAANQCEGGYLEGNRGLGTVDVIPAGVNRRAVMQGKMHYNNLQEDSY
CCCCHHHHHCCCCCCCCCCCCCEEECCCCCCEEEEEECCCCCCEEEECCHHCCCCCCCCC
FPSHEAIDMYHNYKEDIALFAEMGFKCYRFSFSWSRIFPTGKEQEPNEEGLAFYEKFIDE
CCCCHHHHHHHCHHHHHHHHHHCCCEEEEEEEEHHEECCCCCCCCCCHHHHHHHHHHHHH
LLKYNIEPVVTICHFDVPLALEQEYGSWRSRKLIDAYLRYCEAIFKRFNGKVKYWMTFNE
HHHCCCCEEEEEEECCCCEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHH
INMLMHLPFMGAGIMFEEGENELEVKYQAAHNELVASALATKLAKEINKDNKIGCMLAAG
HCEEEECCCCCCCEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEC
QFYPYTCKPEDVLEAMEKDKNNYFFIDVQSRGEYPSYAKKFLERNNIKIEMQSEDEKILR
CCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEECCCHHHHHH
ENTVDFIAFSYYTSRLASSDPNAGEKSAGNVITSLKNPHLKSSKWGWQIDPIGLRITMNS
HCCHHHEEHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEECCEEEEEEHHH
LYDRYQKPLFIVENGLGAVDVLNEDGTVDDDYRIAYLETHIKNMIDAVELDGVELLGYTT
HHHHHCCCEEEEECCCCEEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHCCCEEEEEECH
WGCIDLVSASTGEMKKRYGFIYVDKDNDGHGTLKRYKKKSFYWYKNVIKNNGL
HHHHHHHCCCCCHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SGLAKNFLWGGATAANQCEGGYLEGNRGLGTVDVIPAGVNRRAVMQGKMHYNNLQEDSY
CCCHHHHHCCCCCCCCCCCCCEEECCCCCCEEEEEECCCCCCEEEECCHHCCCCCCCCC
FPSHEAIDMYHNYKEDIALFAEMGFKCYRFSFSWSRIFPTGKEQEPNEEGLAFYEKFIDE
CCCCHHHHHHHCHHHHHHHHHHCCCEEEEEEEEHHEECCCCCCCCCCHHHHHHHHHHHHH
LLKYNIEPVVTICHFDVPLALEQEYGSWRSRKLIDAYLRYCEAIFKRFNGKVKYWMTFNE
HHHCCCCEEEEEEECCCCEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHH
INMLMHLPFMGAGIMFEEGENELEVKYQAAHNELVASALATKLAKEINKDNKIGCMLAAG
HCEEEECCCCCCCEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEC
QFYPYTCKPEDVLEAMEKDKNNYFFIDVQSRGEYPSYAKKFLERNNIKIEMQSEDEKILR
CCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEECCCHHHHHH
ENTVDFIAFSYYTSRLASSDPNAGEKSAGNVITSLKNPHLKSSKWGWQIDPIGLRITMNS
HCCHHHEEHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEECCEEEEEEHHH
LYDRYQKPLFIVENGLGAVDVLNEDGTVDDDYRIAYLETHIKNMIDAVELDGVELLGYTT
HHHHHCCCEEEEECCCCEEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHCCCEEEEEECH
WGCIDLVSASTGEMKKRYGFIYVDKDNDGHGTLKRYKKKSFYWYKNVIKNNGL
HHHHHHHCCCCCHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1630307; 9278503 [H]