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The map label for this gene is potB

Identifier: 170754731

GI number: 170754731

Start: 687374

End: 688333

Strand: Direct

Name: potB

Synonym: CLD_0188

Alternate gene names: 170754731

Gene position: 687374-688333 (Clockwise)

Preceding gene: 170757868

Following gene: 170755689

Centisome position: 17.37

GC content: 29.27

Gene sequence:

>960_bases
ATGTGGGGGAAATTTAGCAATGCTTTTTCAAATATTTTTAAAAAGCAATTGCATAATAAAAATACGGATATTATTTTAGA
AGACAAAGAAGGAATAAGAGGTAAACTAAAAAAATCTACCCCTATATTTAAAACAGTGGGGCCCGTTGCCCTATGGCTCA
TTACATTTTTTATTGTTCCTTTACTTCTTGTATTAGTGGTTAGTTTTCTATCAAGAGGTGAATATGGAGACGTAAGATTA
CCTTTTACACTAAAAAATTATAAAAGACTCATGGATCCTATGTACGGACAAATTTTAATTAAAACTTTAATGATTTCTAT
TAGTACTACTTTAGGGTGTTTGATTATAGGTTATCCTTTTGCATATTTTATTGGAAGATCAAACAAAAAATATAGAGGAA
TACTATTATTATTAGTTATACTACCTTTTTGGACTAATTCTTTAGTTCGAACTTATGCATGGATAATACTTTTAAAAACA
GAGGGAGTAATTAACACATATCTTATGAAATTTGGATTAATTCACTCTCCATTGCAAATGCTTTATACAGATGGTGCTGT
ATTTATAGGAATGCTTTATATAATGTTACCTTTTATGGTGCTACCTATTTATACATCTATAGAAAAATTAGATATGAGTT
ATGTAGAAGCTGCCAATGATCTTGGAGCATCACCTTACAAGACCTTTAAAGAAATAACGTTACCATTAACTCTACCGGGT
ATTATAGCAGGATGTATGCTTGTATTTATACCAACCTTAGGTTATTTCTTTATATCTGACTTAATGGGTGGAAGTAAAAC
TATGTTAATTTCAAATCTAATCAAAAATCAATTTTTAACCTCTAGAGATTGGCCCTTTGGTTCAGCTATAGCAGTAATAT
TAATTATTTTAATGCTTATAGCTATAGGTTTTTATTTTAAAGGAAATAAACCAAAGAAGGGGATAAAGGAGGTTAGCTAA

Upstream 100 bases:

>100_bases
TGGTGTCAGAGCCTATAAGTGATGTGTATTCTCTAGACACCCATAATAAAAACTATGTGTTTGCCACATGGAATCCTAAA
CATGCAGTGGTGATGCATTA

Downstream 100 bases:

>100_bases
TGATGAAGAGCAATAAAGGTTTATTAAGTAAAGTATATGCAACTTTAGTTTATGTTTTTTTATATTTGCCAATCTTTGTT
TTAGTAGTATTTTCTTTTAA

Product: spermidine/putrescine ABC transporter, permease protein PotB

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MWGKFSNAFSNIFKKQLHNKNTDIILEDKEGIRGKLKKSTPIFKTVGPVALWLITFFIVPLLLVLVVSFLSRGEYGDVRL
PFTLKNYKRLMDPMYGQILIKTLMISISTTLGCLIIGYPFAYFIGRSNKKYRGILLLLVILPFWTNSLVRTYAWIILLKT
EGVINTYLMKFGLIHSPLQMLYTDGAVFIGMLYIMLPFMVLPIYTSIEKLDMSYVEAANDLGASPYKTFKEITLPLTLPG
IIAGCMLVFIPTLGYFFISDLMGGSKTMLISNLIKNQFLTSRDWPFGSAIAVILIILMLIAIGFYFKGNKPKKGIKEVS

Sequences:

>Translated_319_residues
MWGKFSNAFSNIFKKQLHNKNTDIILEDKEGIRGKLKKSTPIFKTVGPVALWLITFFIVPLLLVLVVSFLSRGEYGDVRL
PFTLKNYKRLMDPMYGQILIKTLMISISTTLGCLIIGYPFAYFIGRSNKKYRGILLLLVILPFWTNSLVRTYAWIILLKT
EGVINTYLMKFGLIHSPLQMLYTDGAVFIGMLYIMLPFMVLPIYTSIEKLDMSYVEAANDLGASPYKTFKEITLPLTLPG
IIAGCMLVFIPTLGYFFISDLMGGSKTMLISNLIKNQFLTSRDWPFGSAIAVILIILMLIAIGFYFKGNKPKKGIKEVS
>Mature_319_residues
MWGKFSNAFSNIFKKQLHNKNTDIILEDKEGIRGKLKKSTPIFKTVGPVALWLITFFIVPLLLVLVVSFLSRGEYGDVRL
PFTLKNYKRLMDPMYGQILIKTLMISISTTLGCLIIGYPFAYFIGRSNKKYRGILLLLVILPFWTNSLVRTYAWIILLKT
EGVINTYLMKFGLIHSPLQMLYTDGAVFIGMLYIMLPFMVLPIYTSIEKLDMSYVEAANDLGASPYKTFKEITLPLTLPG
IIAGCMLVFIPTLGYFFISDLMGGSKTMLISNLIKNQFLTSRDWPFGSAIAVILIILMLIAIGFYFKGNKPKKGIKEVS

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=259, Percent_Identity=49.4208494208494, Blast_Score=275, Evalue=3e-75,
Organism=Escherichia coli, GI1787080, Length=244, Percent_Identity=45.4918032786885, Blast_Score=221, Evalue=4e-59,
Organism=Escherichia coli, GI1787713, Length=236, Percent_Identity=33.4745762711864, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1788764, Length=183, Percent_Identity=28.9617486338798, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786980, Length=77, Percent_Identity=42.8571428571429, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 36086; Mature: 36086

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWGKFSNAFSNIFKKQLHNKNTDIILEDKEGIRGKLKKSTPIFKTVGPVALWLITFFIVP
CCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHH
LLLVLVVSFLSRGEYGDVRLPFTLKNYKRLMDPMYGQILIKTLMISISTTLGCLIIGYPF
HHHHHHHHHHCCCCCCCEEECEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYFIGRSNKKYRGILLLLVILPFWTNSLVRTYAWIILLKTEGVINTYLMKFGLIHSPLQM
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHH
LYTDGAVFIGMLYIMLPFMVLPIYTSIEKLDMSYVEAANDLGASPYKTFKEITLPLTLPG
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHH
IIAGCMLVFIPTLGYFFISDLMGGSKTMLISNLIKNQFLTSRDWPFGSAIAVILIILMLI
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
AIGFYFKGNKPKKGIKEVS
HHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MWGKFSNAFSNIFKKQLHNKNTDIILEDKEGIRGKLKKSTPIFKTVGPVALWLITFFIVP
CCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHH
LLLVLVVSFLSRGEYGDVRLPFTLKNYKRLMDPMYGQILIKTLMISISTTLGCLIIGYPF
HHHHHHHHHHCCCCCCCEEECEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYFIGRSNKKYRGILLLLVILPFWTNSLVRTYAWIILLKTEGVINTYLMKFGLIHSPLQM
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHH
LYTDGAVFIGMLYIMLPFMVLPIYTSIEKLDMSYVEAANDLGASPYKTFKEITLPLTLPG
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHH
IIAGCMLVFIPTLGYFFISDLMGGSKTMLISNLIKNQFLTSRDWPFGSAIAVILIILMLI
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
AIGFYFKGNKPKKGIKEVS
HHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]