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The map label for this gene is bioB

Identifier: 170754679

GI number: 170754679

Start: 2453941

End: 2454897

Strand: Reverse

Name: bioB

Synonym: CLD_2316

Alternate gene names: 170754679

Gene position: 2454897-2453941 (Counterclockwise)

Preceding gene: 170755678

Following gene: 170755845

Centisome position: 62.02

GC content: 30.3

Gene sequence:

>957_bases
ATGAGTAATATAATTAAATATAAGAAAAAAATTTTAAATGGAGATTTACTAACAAAAGAAGAAGTAGAAGAGCTTTTAGA
GGAAGATATTACAGATCTTGCAGCAACAGCTAATGAAATTAGAGAATCTCTATGTGGAAATAAATTCGACTTATGTACAA
TTATAAATGGGAAGAGTGGAAGGTGCCAAGAAAACTGTAAATATTGTGCTCAATCTGCTCACTTTGACACAGATATAATA
GAGTACAACATCTTAAATAGTGATAGAATTATGAATAGTGCAATATCAAATTATAATAAAGGAGTACATCGTTTTTCAGT
AGTAACCTCAGGTCGTGCACTAAATAATAATGAAGTAGATACATTGTGTAAAACTTATTTAAAATTAAAAGAAACATGTT
CTATTAGACTTTGCGCATCCCATGGTTTATTAAAATATGAAGATCTAAAGAGGTTAAAAGATTCTGGAGTAACGCGTTAT
CATAATAATTTGGAAACCTCAAGAAAATTTTTTACAAAGATATGCACTACCCATAAATATGATGACAAAATTGAAACTAT
AAAAAATGCAAAGAAAGCTGGTTTTGAGATATGTAGTGGTGGAATTATTGGTCTTGGAGAAACCATGGAGGACCGAATCG
ATATGGCTTTTACTTTAAGAGAGCTTTCAGTTGAAAGTGTTCCAGTTAATATTCTTAATCCTATTAAAGGAACACCACTT
GAAAATCAAGAAATTTTATCCTATGAAGAAATAATTAAAACTCTAGCACTTTTCCGTTTTATTTTACCAACAGTACAAAT
AAGGTTAGCTGGAGGTCGTACTATTATAAGTGATAAGGGGAAAAAGGCCTTAGAAAGTGGAGTAAATGGAGCAATTTCTG
GAGATATGCTAACTACTTTAGGAATTGAAACTTCTGAGGACATTAAAATGATAAAGAATTTAGGATTTGAGGTATAA

Upstream 100 bases:

>100_bases
TTATCTTGTTTTCCATTAGACATGCCTGGTGACATATTTCTATGCATAATGAGTGCATTATTTGCTAATAAAATTAACCC
AATTTTAAGGAGAGAAAGAA

Downstream 100 bases:

>100_bases
ATATGGCAAGAGGAATATTTATAACAGCCACAGGAACTGATATTGGAAAAACTTACGTAACGGCTTTAATTATTAAGAGA
TTAAGAGAAACTAATATAAA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MSNIIKYKKKILNGDLLTKEEVEELLEEDITDLAATANEIRESLCGNKFDLCTIINGKSGRCQENCKYCAQSAHFDTDII
EYNILNSDRIMNSAISNYNKGVHRFSVVTSGRALNNNEVDTLCKTYLKLKETCSIRLCASHGLLKYEDLKRLKDSGVTRY
HNNLETSRKFFTKICTTHKYDDKIETIKNAKKAGFEICSGGIIGLGETMEDRIDMAFTLRELSVESVPVNILNPIKGTPL
ENQEILSYEEIIKTLALFRFILPTVQIRLAGGRTIISDKGKKALESGVNGAISGDMLTTLGIETSEDIKMIKNLGFEV

Sequences:

>Translated_318_residues
MSNIIKYKKKILNGDLLTKEEVEELLEEDITDLAATANEIRESLCGNKFDLCTIINGKSGRCQENCKYCAQSAHFDTDII
EYNILNSDRIMNSAISNYNKGVHRFSVVTSGRALNNNEVDTLCKTYLKLKETCSIRLCASHGLLKYEDLKRLKDSGVTRY
HNNLETSRKFFTKICTTHKYDDKIETIKNAKKAGFEICSGGIIGLGETMEDRIDMAFTLRELSVESVPVNILNPIKGTPL
ENQEILSYEEIIKTLALFRFILPTVQIRLAGGRTIISDKGKKALESGVNGAISGDMLTTLGIETSEDIKMIKNLGFEV
>Mature_317_residues
SNIIKYKKKILNGDLLTKEEVEELLEEDITDLAATANEIRESLCGNKFDLCTIINGKSGRCQENCKYCAQSAHFDTDIIE
YNILNSDRIMNSAISNYNKGVHRFSVVTSGRALNNNEVDTLCKTYLKLKETCSIRLCASHGLLKYEDLKRLKDSGVTRYH
NNLETSRKFFTKICTTHKYDDKIETIKNAKKAGFEICSGGIIGLGETMEDRIDMAFTLRELSVESVPVNILNPIKGTPLE
NQEILSYEEIIKTLALFRFILPTVQIRLAGGRTIISDKGKKALESGVNGAISGDMLTTLGIETSEDIKMIKNLGFEV

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=305, Percent_Identity=30.1639344262295, Blast_Score=169, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6321725, Length=314, Percent_Identity=32.8025477707006, Blast_Score=177, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 35584; Mature: 35453

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNIIKYKKKILNGDLLTKEEVEELLEEDITDLAATANEIRESLCGNKFDLCTIINGKSG
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCC
RCQENCKYCAQSAHFDTDIIEYNILNSDRIMNSAISNYNKGVHRFSVVTSGRALNNNEVD
CCHHHHHHHHHHCCCCCHHEEEEECCHHHHHHHHHHHHHCCHHEEEEEECCCCCCCCHHH
TLCKTYLKLKETCSIRLCASHGLLKYEDLKRLKDSGVTRYHNNLETSRKFFTKICTTHKY
HHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
DDKIETIKNAKKAGFEICSGGIIGLGETMEDRIDMAFTLRELSVESVPVNILNPIKGTPL
CHHHHHHHHHHHHCHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCC
ENQEILSYEEIIKTLALFRFILPTVQIRLAGGRTIISDKGKKALESGVNGAISGDMLTTL
CCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEECCCCHHHHHHCCCCCCCCCHHHHC
GIETSEDIKMIKNLGFEV
CCCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
SNIIKYKKKILNGDLLTKEEVEELLEEDITDLAATANEIRESLCGNKFDLCTIINGKSG
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCC
RCQENCKYCAQSAHFDTDIIEYNILNSDRIMNSAISNYNKGVHRFSVVTSGRALNNNEVD
CCHHHHHHHHHHCCCCCHHEEEEECCHHHHHHHHHHHHHCCHHEEEEEECCCCCCCCHHH
TLCKTYLKLKETCSIRLCASHGLLKYEDLKRLKDSGVTRYHNNLETSRKFFTKICTTHKY
HHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
DDKIETIKNAKKAGFEICSGGIIGLGETMEDRIDMAFTLRELSVESVPVNILNPIKGTPL
CHHHHHHHHHHHHCHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCC
ENQEILSYEEIIKTLALFRFILPTVQIRLAGGRTIISDKGKKALESGVNGAISGDMLTTL
CCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEECCCCHHHHHHCCCCCCCCCHHHHC
GIETSEDIKMIKNLGFEV
CCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA