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The map label for this gene is folD

Identifier: 170754644

GI number: 170754644

Start: 1298534

End: 1299382

Strand: Direct

Name: folD

Synonym: CLD_3397

Alternate gene names: 170754644

Gene position: 1298534-1299382 (Clockwise)

Preceding gene: 170756609

Following gene: 170754328

Centisome position: 32.81

GC content: 26.27

Gene sequence:

>849_bases
ATGACTAAAATATTATATGGAAATGAAGTAGCTTTAAAAATAAAGGAAGATTTAAATTTAAGAATGGACAAATTAAAAGA
AAAAAATATAATACCTAAGTTAGCAATTTTACGTATGGGAAATAAACAGGACGATATAGCCTATGAAAGAAGTATAATAA
AAAGCTGTGAAAAACTAAACATAGAAACTAAGGTAGAAGAATTAAATGAAGATATATTAGAAGAAGATTTTTTAAAGTTA
ATGGAAAATTTAAATAATGAAAAGGATATTCATGGTATATTAGTCTTTAGACCTTACCCTAAACATTTAAATGAAAATAT
AATAAACTCTTCTATAGCATTAAATAAAGATGTGGATTGTATGCATCCTTTAAATTTAGAAAGGATATTTGAAGGTGATT
TAGATGGAGTTATGCCTTGTACTCCAGAGGCTGTAATAGAAATATTAAAATATTATGATATAGATTTAAAAGGAAAGAAT
ATAGTTATTATAAACAGAAGTATGGTAGTGGGCAAACCATTGAGTATGATGGTCTTATCTAATAATGCTACAGTTACTAT
ATGTCATTCAAAAACTATAGATTTACCATCCATAACTAAAAAAGCAGATATAGTAGTGACAGCTATAGGAAAAGCTAAAT
TAATAAAAGAGGAATATTTTAATGAAAATTCTATAATTATAGATGTAAGTATTAATGTAGATGAAAATGGAAAACTATGT
GGAGATGTGGATTTTGAAAATGTAAAAGAAAAAGTAGGAGCTATAACTCCAGTTCCAAAAGGAGTAGGAAGTGTTACAAC
TACCTTGTTATTAAAACACATAGTAGAAGCAGCAGAGAGAAATAGTTAA

Upstream 100 bases:

>100_bases
ATTCTTTGGAAGAAGAAATCAACACCTTAGTTTCTAAATATTCAACTAAGGCAGATAAAGTTTATAGTTATGTAGAAAAT
TTAATAAGGGGAAATGAATA

Downstream 100 bases:

>100_bases
ATATAAAAATTAAAAAGTAAAGCCTGTATCTTATGTTAGTTTATATAGGATACAGGATTATTTTTCTTATAATAAATAAT
ATATAAGATTTTATAGATTC

Product: methylenetetrahydrofolate dehydrogenase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MTKILYGNEVALKIKEDLNLRMDKLKEKNIIPKLAILRMGNKQDDIAYERSIIKSCEKLNIETKVEELNEDILEEDFLKL
MENLNNEKDIHGILVFRPYPKHLNENIINSSIALNKDVDCMHPLNLERIFEGDLDGVMPCTPEAVIEILKYYDIDLKGKN
IVIINRSMVVGKPLSMMVLSNNATVTICHSKTIDLPSITKKADIVVTAIGKAKLIKEEYFNENSIIIDVSINVDENGKLC
GDVDFENVKEKVGAITPVPKGVGSVTTTLLLKHIVEAAERNS

Sequences:

>Translated_282_residues
MTKILYGNEVALKIKEDLNLRMDKLKEKNIIPKLAILRMGNKQDDIAYERSIIKSCEKLNIETKVEELNEDILEEDFLKL
MENLNNEKDIHGILVFRPYPKHLNENIINSSIALNKDVDCMHPLNLERIFEGDLDGVMPCTPEAVIEILKYYDIDLKGKN
IVIINRSMVVGKPLSMMVLSNNATVTICHSKTIDLPSITKKADIVVTAIGKAKLIKEEYFNENSIIIDVSINVDENGKLC
GDVDFENVKEKVGAITPVPKGVGSVTTTLLLKHIVEAAERNS
>Mature_281_residues
TKILYGNEVALKIKEDLNLRMDKLKEKNIIPKLAILRMGNKQDDIAYERSIIKSCEKLNIETKVEELNEDILEEDFLKLM
ENLNNEKDIHGILVFRPYPKHLNENIINSSIALNKDVDCMHPLNLERIFEGDLDGVMPCTPEAVIEILKYYDIDLKGKNI
VIINRSMVVGKPLSMMVLSNNATVTICHSKTIDLPSITKKADIVVTAIGKAKLIKEEYFNENSIIIDVSINVDENGKLCG
DVDFENVKEKVGAITPVPKGVGSVTTTLLLKHIVEAAERNS

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222136639, Length=293, Percent_Identity=36.518771331058, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI222418558, Length=293, Percent_Identity=36.1774744027304, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI94721354, Length=293, Percent_Identity=34.1296928327645, Blast_Score=143, Evalue=1e-34,
Organism=Escherichia coli, GI1786741, Length=277, Percent_Identity=33.5740072202166, Blast_Score=168, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17568735, Length=291, Percent_Identity=32.6460481099656, Blast_Score=153, Evalue=9e-38,
Organism=Saccharomyces cerevisiae, GI6319558, Length=287, Percent_Identity=36.9337979094077, Blast_Score=200, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6321643, Length=269, Percent_Identity=36.8029739776952, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6322933, Length=251, Percent_Identity=22.3107569721116, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI62472483, Length=288, Percent_Identity=34.375, Blast_Score=181, Evalue=4e-46,
Organism=Drosophila melanogaster, GI45551871, Length=288, Percent_Identity=34.375, Blast_Score=181, Evalue=4e-46,
Organism=Drosophila melanogaster, GI24645718, Length=288, Percent_Identity=34.375, Blast_Score=181, Evalue=5e-46,
Organism=Drosophila melanogaster, GI17137370, Length=288, Percent_Identity=34.375, Blast_Score=181, Evalue=5e-46,
Organism=Drosophila melanogaster, GI17136818, Length=297, Percent_Identity=31.6498316498317, Blast_Score=154, Evalue=8e-38,
Organism=Drosophila melanogaster, GI17136816, Length=297, Percent_Identity=31.6498316498317, Blast_Score=153, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 31685; Mature: 31554

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKILYGNEVALKIKEDLNLRMDKLKEKNIIPKLAILRMGNKQDDIAYERSIIKSCEKLN
CCEEEECCEEEEEEECCCCHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCC
IETKVEELNEDILEEDFLKLMENLNNEKDIHGILVFRPYPKHLNENIINSSIALNKDVDC
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHCCCEEECCCCCC
MHPLNLERIFEGDLDGVMPCTPEAVIEILKYYDIDLKGKNIVIINRSMVVGKPLSMMVLS
CCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHCCEECCCEEEEEECCEEECCCEEEEEEE
NNATVTICHSKTIDLPSITKKADIVVTAIGKAKLIKEEYFNENSIIIDVSINVDENGKLC
CCCEEEEEECCCCCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCEEEEEEEEECCCCCEE
GDVDFENVKEKVGAITPVPKGVGSVTTTLLLKHIVEAAERNS
CCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKILYGNEVALKIKEDLNLRMDKLKEKNIIPKLAILRMGNKQDDIAYERSIIKSCEKLN
CEEEECCEEEEEEECCCCHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCC
IETKVEELNEDILEEDFLKLMENLNNEKDIHGILVFRPYPKHLNENIINSSIALNKDVDC
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHCCCEEECCCCCC
MHPLNLERIFEGDLDGVMPCTPEAVIEILKYYDIDLKGKNIVIINRSMVVGKPLSMMVLS
CCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHCCEECCCEEEEEECCEEECCCEEEEEEE
NNATVTICHSKTIDLPSITKKADIVVTAIGKAKLIKEEYFNENSIIIDVSINVDENGKLC
CCCEEEEEECCCCCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCEEEEEEEEECCCCCEE
GDVDFENVKEKVGAITPVPKGVGSVTTTLLLKHIVEAAERNS
CCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA