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The map label for this gene is azoB [H]

Identifier: 170754481

GI number: 170754481

Start: 2370026

End: 2370886

Strand: Reverse

Name: azoB [H]

Synonym: CLD_2396

Alternate gene names: 170754481

Gene position: 2370886-2370026 (Counterclockwise)

Preceding gene: 170757164

Following gene: 170755387

Centisome position: 59.9

GC content: 36.7

Gene sequence:

>861_bases
ATGATACTTATTACTGGAGCAAATGGGCAAACTGGTCGTGCAATAATTAAGGCTTTATTATCTAAAGGTGAGCGGATTAG
GGCTTTCGTTCATACAACTGAACAAATTCAGGAAATTAAATCCTTAGGTGAGATGGAAGTAGTTGCAGGAGACATGATGA
ATCAAAGGGACGTAGAGGAAGCATTTATCGGAGTCAGCGCTGTATACCACATTTGTTCTGCTGTAAACCCCAATGAAGTT
GAGATAGAACAAATGGCAATAAACGCTGCTCGCAAGGCTAAAGTAGAGCATTTCGTATATCATTCAGTATTGCATTCTGT
ACTTCAAGATATGCTACATCATCAGAAAAAACTCAAGGTTGAGGAACTTCTAGTAAATTCAGCAATCCCATATACTATTA
TTCAGCCAGCAGTATTTATGCAGAATATACTAGAGTCTTGGAATTCACTTAGTGAAAAAGGCATATTTCAACAGAAGTTT
TTTACTACTCAAGAAACTCGCATGTGTATGGTTGACTTAGAAGACTTAGCCGAAGCTGTTTCAATAATTCTCACAAGTCC
AGGTCATACGGGGGCTACATATGAACTTTGTGGACCTGAAGATTTATCTTTGTTTGATATGATAGCTACTATGGAACAGC
ATATTGGTCTTAAAATCAAAGTCGAAACTCCTCAAGATGAAATGTTCGCAGCACAATTAAAAAAGCTTGGTGTAGGAGAC
TATCAAGTTAATACACTATTAAAGATGTTTCAACATTATAATGAACATGGATTCATCGGTAATCCAAATGTTTTGACGTG
GATATTAGGTCGAAGGCCAAATGATTTTTCATCCTTCATTCTTAGAACATTGAGAAGCTAA

Upstream 100 bases:

>100_bases
CTAATGGCTGGACTTGAGGATGGTCAATTGAAGGAATTAACCACTTTACTTAATCTATTATCAAAGAATTTATTATGATA
TAGAAAGGTGAGATATTATT

Downstream 100 bases:

>100_bases
ATATAGTAGTTGTTAATTAAAATCCTAGAGCAAAGAAATAATATAAGATAATATAATGGGAGGTAATAAATTATGAATTT
TTGCTGGATAACATTAAATG

Product: putative nucleoside-diphosphate-sugar epimerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MILITGANGQTGRAIIKALLSKGERIRAFVHTTEQIQEIKSLGEMEVVAGDMMNQRDVEEAFIGVSAVYHICSAVNPNEV
EIEQMAINAARKAKVEHFVYHSVLHSVLQDMLHHQKKLKVEELLVNSAIPYTIIQPAVFMQNILESWNSLSEKGIFQQKF
FTTQETRMCMVDLEDLAEAVSIILTSPGHTGATYELCGPEDLSLFDMIATMEQHIGLKIKVETPQDEMFAAQLKKLGVGD
YQVNTLLKMFQHYNEHGFIGNPNVLTWILGRRPNDFSSFILRTLRS

Sequences:

>Translated_286_residues
MILITGANGQTGRAIIKALLSKGERIRAFVHTTEQIQEIKSLGEMEVVAGDMMNQRDVEEAFIGVSAVYHICSAVNPNEV
EIEQMAINAARKAKVEHFVYHSVLHSVLQDMLHHQKKLKVEELLVNSAIPYTIIQPAVFMQNILESWNSLSEKGIFQQKF
FTTQETRMCMVDLEDLAEAVSIILTSPGHTGATYELCGPEDLSLFDMIATMEQHIGLKIKVETPQDEMFAAQLKKLGVGD
YQVNTLLKMFQHYNEHGFIGNPNVLTWILGRRPNDFSSFILRTLRS
>Mature_286_residues
MILITGANGQTGRAIIKALLSKGERIRAFVHTTEQIQEIKSLGEMEVVAGDMMNQRDVEEAFIGVSAVYHICSAVNPNEV
EIEQMAINAARKAKVEHFVYHSVLHSVLQDMLHHQKKLKVEELLVNSAIPYTIIQPAVFMQNILESWNSLSEKGIFQQKF
FTTQETRMCMVDLEDLAEAVSIILTSPGHTGATYELCGPEDLSLFDMIATMEQHIGLKIKVETPQDEMFAAQLKKLGVGD
YQVNTLLKMFQHYNEHGFIGNPNVLTWILGRRPNDFSSFILRTLRS

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Uses preferentially NADPH rather than NADH as an electron donor for its activity. The enzyme reductively cleaved Orange II and carboxy-Orange II, and can a

COG id: COG0702

COG function: function code MG; Predicted nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NmrA-type oxidoreductase family. Azoreductase type 3 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790656, Length=272, Percent_Identity=25.3676470588235, Blast_Score=85, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR008030 [H]

Pfam domain/function: PF05368 NmrA [H]

EC number: NA

Molecular weight: Translated: 32235; Mature: 32235

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILITGANGQTGRAIIKALLSKGERIRAFVHTTEQIQEIKSLGEMEVVAGDMMNQRDVEE
CEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHH
AFIGVSAVYHICSAVNPNEVEIEQMAINAARKAKVEHFVYHSVLHSVLQDMLHHQKKLKV
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EELLVNSAIPYTIIQPAVFMQNILESWNSLSEKGIFQQKFFTTQETRMCMVDLEDLAEAV
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHH
SIILTSPGHTGATYELCGPEDLSLFDMIATMEQHIGLKIKVETPQDEMFAAQLKKLGVGD
HHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHCCCCC
YQVNTLLKMFQHYNEHGFIGNPNVLTWILGRRPNDFSSFILRTLRS
HHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MILITGANGQTGRAIIKALLSKGERIRAFVHTTEQIQEIKSLGEMEVVAGDMMNQRDVEE
CEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHH
AFIGVSAVYHICSAVNPNEVEIEQMAINAARKAKVEHFVYHSVLHSVLQDMLHHQKKLKV
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EELLVNSAIPYTIIQPAVFMQNILESWNSLSEKGIFQQKFFTTQETRMCMVDLEDLAEAV
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHH
SIILTSPGHTGATYELCGPEDLSLFDMIATMEQHIGLKIKVETPQDEMFAAQLKKLGVGD
HHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHCCCCC
YQVNTLLKMFQHYNEHGFIGNPNVLTWILGRRPNDFSSFILRTLRS
HHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12147495 [H]