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The map label for this gene is dnaG

Identifier: 170754369

GI number: 170754369

Start: 3185163

End: 3186935

Strand: Reverse

Name: dnaG

Synonym: CLD_1607

Alternate gene names: 170754369

Gene position: 3186935-3185163 (Counterclockwise)

Preceding gene: 170755081

Following gene: 170757237

Centisome position: 80.51

GC content: 27.81

Gene sequence:

>1773_bases
TTGATATCGGAAGATGTCGTCCAAAAGGTAATAGAGCTAAACGATATTGTAGATGTTATATCAGGGGACATAAAGCTTAA
AAACAGTGGAAGAAACTATTTTGGGTTATGTCCTTTTCATCATGAAAAAACACCTTCCTTTAGTGTGTCGCAGGATAAGC
AGATATATAAATGTTTTGGATGCGGAGAAGCAGGAAACGTTGTGACTTATGTAATGAAGACTAAAAACGTTGCTTTCCCA
GAGGCCATAAAAATTTTAGCAGAAAGAGTAAATATAGATATAGAGGAAGATAAAAAAGAAAATACAAATAATCCTAAAGA
TAAAATCTATAAAATAAATGTAGACGCAGCTAGATATTTTTTTAATAATCTTATAATAAATAAAAATGCTATAAATTACT
TTTTAAATAGGGGTGTTACAAAGCCAATAATTAAAAGGTTTGGATTAGGATATTCAAAGGATAGTTGGGATGGGCTTTTA
AGGCATTTAAAAACAAAAGGCTATACGGAACTAGATATGCTATCTGCAGGTCTTATTATAAAGAGTAAAAATGGATCTTA
TTATGATAGATTTAGAAACAGAGTTATGTTTCCAGTCTTTGATTATAGAGGAAAGGTAATAGGCTTTGGAGGAAGGGTAC
TAAATAACGCTAAACCTAAATATTTAAACTCACCAGAAACTATGGTGTTTAAAAAGGGTATAAATTTATATGGATTAAAT
TATGCAGCAAAAGAAAATAAAGATAGAGTATTAATAATAGTAGAAGGATATATGGATTGCATTACATTACATCAATATGG
CATAAAAAATTCTGTAGCATCTCTAGGAACAGCTCTAACTACAAATCAAGCTAGACTTTTAAAACGATATGCAGATAAAG
TCATAATATCTTATGACTCAGATACAGCAGGGCAATTAGCGACTCAAAGGGGCTTAGAAATATTAAAAGATGTAGGATTA
GGTGTAGAAATATTAACTGTACCAGATGGGAAAGATCCAGATCAATTTGTAAAGGCTCATGGAAAAGAATCTTACTTAAA
GTTAGTAAAAGAAGCTTTACCTCTTATAGAGTATAAAATAAAAATTAGTAAGCAGGATTTAAATATTGGAGATAAAAGGC
AAGCTATACAGTATATAGATAGATATATTAAAATTATAGAAAATTTAGATCCTGTAGAAAAAGATATGTACATAAGAAGA
CTTTCAGAGGAAACTAATATAGAAATTCAGACTTTATATGACCAATTAAACAAAAAAAACCAAAATTCTAGGAAAAATGG
AAAAGAAGTGAATATGTTAGAAGCTTTTGGACAAAAATTGTATCTAGAGCCAGCTTATATAAGATCTGAGAGATTTATCC
TGCAATTAATGTTTAATTATAATAATATTTATGAATATATAAAGAATAGTATAAATGAGCAGGATATAATAGAGGAAACA
CATAAGGCAATATATAAATATATAAAAGATACTTTAGAAGACTCAAATATACAAAATAAAAAATTACACATAGAAACTCT
ATGTAATAAGAATGTAGAAACATCTAAAGAATGGGTAAATATATTAAATACCAATTTTATTTACAAAGAAGATGAAAGTA
AAAAATTTATAGATGATGCTATTTTAAATATTAAAAAATATAAATTAGAAAAATATAAGGAAGATTTACTTAAAAAGATT
AAAGAGTATGAAGCACAAGGAAAATTAGATGAAACTATAAAAATGAGCCAGGAACTCATCAAAGTAAAGAAAACCTTAGG
GGCCATGAAATAA

Upstream 100 bases:

>100_bases
AGAAGGAAATATAAAGTTTTTGTCGAATATATTATTTTTACCTATGTATTTTTAAAATAATATATTCATAAAATATTAAA
GAGTAAGGTGGGAGTAGCCA

Downstream 100 bases:

>100_bases
TGGCGAAAGGAGGCTACACTTATAATGAAAGAAAAGAAGAATAATGCTAAAGATAAAAATGAAAAAATGAAGTTAGTTAG
ACGTTTAATAGATAAAGGTA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 590; Mature: 590

Protein sequence:

>590_residues
MISEDVVQKVIELNDIVDVISGDIKLKNSGRNYFGLCPFHHEKTPSFSVSQDKQIYKCFGCGEAGNVVTYVMKTKNVAFP
EAIKILAERVNIDIEEDKKENTNNPKDKIYKINVDAARYFFNNLIINKNAINYFLNRGVTKPIIKRFGLGYSKDSWDGLL
RHLKTKGYTELDMLSAGLIIKSKNGSYYDRFRNRVMFPVFDYRGKVIGFGGRVLNNAKPKYLNSPETMVFKKGINLYGLN
YAAKENKDRVLIIVEGYMDCITLHQYGIKNSVASLGTALTTNQARLLKRYADKVIISYDSDTAGQLATQRGLEILKDVGL
GVEILTVPDGKDPDQFVKAHGKESYLKLVKEALPLIEYKIKISKQDLNIGDKRQAIQYIDRYIKIIENLDPVEKDMYIRR
LSEETNIEIQTLYDQLNKKNQNSRKNGKEVNMLEAFGQKLYLEPAYIRSERFILQLMFNYNNIYEYIKNSINEQDIIEET
HKAIYKYIKDTLEDSNIQNKKLHIETLCNKNVETSKEWVNILNTNFIYKEDESKKFIDDAILNIKKYKLEKYKEDLLKKI
KEYEAQGKLDETIKMSQELIKVKKTLGAMK

Sequences:

>Translated_590_residues
MISEDVVQKVIELNDIVDVISGDIKLKNSGRNYFGLCPFHHEKTPSFSVSQDKQIYKCFGCGEAGNVVTYVMKTKNVAFP
EAIKILAERVNIDIEEDKKENTNNPKDKIYKINVDAARYFFNNLIINKNAINYFLNRGVTKPIIKRFGLGYSKDSWDGLL
RHLKTKGYTELDMLSAGLIIKSKNGSYYDRFRNRVMFPVFDYRGKVIGFGGRVLNNAKPKYLNSPETMVFKKGINLYGLN
YAAKENKDRVLIIVEGYMDCITLHQYGIKNSVASLGTALTTNQARLLKRYADKVIISYDSDTAGQLATQRGLEILKDVGL
GVEILTVPDGKDPDQFVKAHGKESYLKLVKEALPLIEYKIKISKQDLNIGDKRQAIQYIDRYIKIIENLDPVEKDMYIRR
LSEETNIEIQTLYDQLNKKNQNSRKNGKEVNMLEAFGQKLYLEPAYIRSERFILQLMFNYNNIYEYIKNSINEQDIIEET
HKAIYKYIKDTLEDSNIQNKKLHIETLCNKNVETSKEWVNILNTNFIYKEDESKKFIDDAILNIKKYKLEKYKEDLLKKI
KEYEAQGKLDETIKMSQELIKVKKTLGAMK
>Mature_590_residues
MISEDVVQKVIELNDIVDVISGDIKLKNSGRNYFGLCPFHHEKTPSFSVSQDKQIYKCFGCGEAGNVVTYVMKTKNVAFP
EAIKILAERVNIDIEEDKKENTNNPKDKIYKINVDAARYFFNNLIINKNAINYFLNRGVTKPIIKRFGLGYSKDSWDGLL
RHLKTKGYTELDMLSAGLIIKSKNGSYYDRFRNRVMFPVFDYRGKVIGFGGRVLNNAKPKYLNSPETMVFKKGINLYGLN
YAAKENKDRVLIIVEGYMDCITLHQYGIKNSVASLGTALTTNQARLLKRYADKVIISYDSDTAGQLATQRGLEILKDVGL
GVEILTVPDGKDPDQFVKAHGKESYLKLVKEALPLIEYKIKISKQDLNIGDKRQAIQYIDRYIKIIENLDPVEKDMYIRR
LSEETNIEIQTLYDQLNKKNQNSRKNGKEVNMLEAFGQKLYLEPAYIRSERFILQLMFNYNNIYEYIKNSINEQDIIEET
HKAIYKYIKDTLEDSNIQNKKLHIETLCNKNVETSKEWVNILNTNFIYKEDESKKFIDDAILNIKKYKLEKYKEDLLKKI
KEYEAQGKLDETIKMSQELIKVKKTLGAMK

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=392, Percent_Identity=37.7551020408163, Blast_Score=289, Evalue=3e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 68265; Mature: 68265

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISEDVVQKVIELNDIVDVISGDIKLKNSGRNYFGLCPFHHEKTPSFSVSQDKQIYKCFG
CCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEECCCCCCCCCCCCCCCCCHHHHHCC
CGEAGNVVTYVMKTKNVAFPEAIKILAERVNIDIEEDKKENTNNPKDKIYKINVDAARYF
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEHHHHHHH
FNNLIINKNAINYFLNRGVTKPIIKRFGLGYSKDSWDGLLRHLKTKGYTELDMLSAGLII
HHHHEECCHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCEEE
KSKNGSYYDRFRNRVMFPVFDYRGKVIGFGGRVLNNAKPKYLNSPETMVFKKGINLYGLN
ECCCCCHHHHHHCCEEEEEECCCCCEEEECCHHHCCCCCCCCCCCCHHHHHCCCCEEECC
YAAKENKDRVLIIVEGYMDCITLHQYGIKNSVASLGTALTTNQARLLKRYADKVIISYDS
CCCCCCCCEEEEEECCHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHHHHCEEEEEECC
DTAGQLATQRGLEILKDVGLGVEILTVPDGKDPDQFVKAHGKESYLKLVKEALPLIEYKI
CCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHCCHHHHHHHHHHHHHHEEEEE
KISKQDLNIGDKRQAIQYIDRYIKIIENLDPVEKDMYIRRLSEETNIEIQTLYDQLNKKN
EEECCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCC
QNSRKNGKEVNMLEAFGQKLYLEPAYIRSERFILQLMFNYNNIYEYIKNSINEQDIIEET
CCCCCCCCCCHHHHHHCHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
HKAIYKYIKDTLEDSNIQNKKLHIETLCNKNVETSKEWVNILNTNFIYKEDESKKFIDDA
HHHHHHHHHHHHCCCCCCCCEEEHHHHHCCCCCCHHHHHHHHCCCCEEECCCCHHHHHHH
ILNIKKYKLEKYKEDLLKKIKEYEAQGKLDETIKMSQELIKVKKTLGAMK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MISEDVVQKVIELNDIVDVISGDIKLKNSGRNYFGLCPFHHEKTPSFSVSQDKQIYKCFG
CCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEECCCCCCCCCCCCCCCCCHHHHHCC
CGEAGNVVTYVMKTKNVAFPEAIKILAERVNIDIEEDKKENTNNPKDKIYKINVDAARYF
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEHHHHHHH
FNNLIINKNAINYFLNRGVTKPIIKRFGLGYSKDSWDGLLRHLKTKGYTELDMLSAGLII
HHHHEECCHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCEEE
KSKNGSYYDRFRNRVMFPVFDYRGKVIGFGGRVLNNAKPKYLNSPETMVFKKGINLYGLN
ECCCCCHHHHHHCCEEEEEECCCCCEEEECCHHHCCCCCCCCCCCCHHHHHCCCCEEECC
YAAKENKDRVLIIVEGYMDCITLHQYGIKNSVASLGTALTTNQARLLKRYADKVIISYDS
CCCCCCCCEEEEEECCHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHHHHCEEEEEECC
DTAGQLATQRGLEILKDVGLGVEILTVPDGKDPDQFVKAHGKESYLKLVKEALPLIEYKI
CCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHCCHHHHHHHHHHHHHHEEEEE
KISKQDLNIGDKRQAIQYIDRYIKIIENLDPVEKDMYIRRLSEETNIEIQTLYDQLNKKN
EEECCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCC
QNSRKNGKEVNMLEAFGQKLYLEPAYIRSERFILQLMFNYNNIYEYIKNSINEQDIIEET
CCCCCCCCCCHHHHHHCHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
HKAIYKYIKDTLEDSNIQNKKLHIETLCNKNVETSKEWVNILNTNFIYKEDESKKFIDDA
HHHHHHHHHHHHCCCCCCCCEEEHHHHHCCCCCCHHHHHHHHCCCCEEECCCCHHHHHHH
ILNIKKYKLEKYKEDLLKKIKEYEAQGKLDETIKMSQELIKVKKTLGAMK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7961408; 11466286 [H]