Click here to switch to the map view.

The map label for this gene is adaA [H]

Identifier: 170754344

GI number: 170754344

Start: 759252

End: 759800

Strand: Reverse

Name: adaA [H]

Synonym: CLD_0121

Alternate gene names: 170754344

Gene position: 759800-759252 (Counterclockwise)

Preceding gene: 170757241

Following gene: 170755012

Centisome position: 19.2

GC content: 43.72

Gene sequence:

>549_bases
ATGATTGAGGAAGAAATGTGGAAAGCAGTATCCGAAAATGATGTAGCCTATGACGGTATCTTTTTCTACGCAGTCAAGTC
CACTGGAATTTATTGCCGCCCCTCCTGTAAATCAAAAAGACCCAAACGTGAAAATGTGCGCTTCTTTGATACTGCGGATC
AAGCGAGAGCTGCAGGCTTTCGTCCCTGTAAACGATGCAGAAGCGACCTTTTAGACTACCAGCCAATCAAAGAAATTGCA
GAAAAGGCAAAGCGGCTTCTGGACGATTATTTTCAAAAAAATCAGGAACTAAATCAGGAACTGCGTCATTTGGGCATATC
CCAGCGGCGCATGGTTGAAATTTTCAAGGACGAATATGGTGTTACTTTGTCCGAATATGTGGGCAATCTGCGCTTGACAG
AAGCAAAACGACTGCTATCAGACACCAATGACGAAATTGTCAACATTGCTTACTCTGTAGGATTCTCCGGTCTATCATCC
TTTTACCGCTTTTTCAAAACTGGTACCGGATTGTCTCCTGCCGCTTATAGAAAGGAGCATCGTCAATGA

Upstream 100 bases:

>100_bases
ATGGAACGGTAGCAGGCCTGTATCTTTGGGCGATTGCCGGGGGTGCGATTCCGGAAATGCGGGATTATGCGCCAAAAAAG
AAAAAGGAGGTGACAAAGAA

Downstream 100 bases:

>100_bases
TAAGGTACGCATTTTACGATTTTGAATTTGGAACTCTAAAAATAGGATATACCGATGAAAGAGTCGTCTTCTTGAAGCGG
GTGGAACAAATCAACGCAGA

Product: methylphosphotriester-DNA alkyltransferase

Products: DNA; protein S-methyl-L-cysteine

Alternate protein names: NA

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MIEEEMWKAVSENDVAYDGIFFYAVKSTGIYCRPSCKSKRPKRENVRFFDTADQARAAGFRPCKRCRSDLLDYQPIKEIA
EKAKRLLDDYFQKNQELNQELRHLGISQRRMVEIFKDEYGVTLSEYVGNLRLTEAKRLLSDTNDEIVNIAYSVGFSGLSS
FYRFFKTGTGLSPAAYRKEHRQ

Sequences:

>Translated_182_residues
MIEEEMWKAVSENDVAYDGIFFYAVKSTGIYCRPSCKSKRPKRENVRFFDTADQARAAGFRPCKRCRSDLLDYQPIKEIA
EKAKRLLDDYFQKNQELNQELRHLGISQRRMVEIFKDEYGVTLSEYVGNLRLTEAKRLLSDTNDEIVNIAYSVGFSGLSS
FYRFFKTGTGLSPAAYRKEHRQ
>Mature_182_residues
MIEEEMWKAVSENDVAYDGIFFYAVKSTGIYCRPSCKSKRPKRENVRFFDTADQARAAGFRPCKRCRSDLLDYQPIKEIA
EKAKRLLDDYFQKNQELNQELRHLGISQRRMVEIFKDEYGVTLSEYVGNLRLTEAKRLLSDTNDEIVNIAYSVGFSGLSS
FYRFFKTGTGLSPAAYRKEHRQ

Specific function: Methylphosphotriester-DNA alkyltransferase is one of two genes required for the adaptive response of bacteria to alkylating agents. It accepts a methyl group from methylphospho-triesters and then acts as a transcriptional activator of the ada operon [H]

COG id: COG2169

COG function: function code F; Adenosine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788542, Length=185, Percent_Identity=31.3513513513514, Blast_Score=92, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR016220 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF00165 HTH_AraC [H]

EC number: 2.1.1.63

Molecular weight: Translated: 21198; Mature: 21198

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEEEMWKAVSENDVAYDGIFFYAVKSTGIYCRPSCKSKRPKRENVRFFDTADQARAAGF
CCHHHHHHHHCCCCCEECCEEEEEEECCCEEECCCCCCCCCCCCCCCEECCHHHHHHCCC
RPCKRCRSDLLDYQPIKEIAEKAKRLLDDYFQKNQELNQELRHLGISQRRMVEIFKDEYG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
VTLSEYVGNLRLTEAKRLLSDTNDEIVNIAYSVGFSGLSSFYRFFKTGTGLSPAAYRKEH
CCHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHHHHHC
RQ
CC
>Mature Secondary Structure
MIEEEMWKAVSENDVAYDGIFFYAVKSTGIYCRPSCKSKRPKRENVRFFDTADQARAAGF
CCHHHHHHHHCCCCCEECCEEEEEEECCCEEECCCCCCCCCCCCCCCEECCHHHHHHCCC
RPCKRCRSDLLDYQPIKEIAEKAKRLLDDYFQKNQELNQELRHLGISQRRMVEIFKDEYG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
VTLSEYVGNLRLTEAKRLLSDTNDEIVNIAYSVGFSGLSSFYRFFKTGTGLSPAAYRKEH
CCHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHHHHHC
RQ
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA containing 6-O-methylguanine; protein L-cysteine

Specific reaction: DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2120677; 9384377; 1744039 [H]