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The map label for this gene is pinE [H]

Identifier: 170752072

GI number: 170752072

Start: 6030161

End: 6031012

Strand: Reverse

Name: pinE [H]

Synonym: Mrad2831_5704

Alternate gene names: 170752072

Gene position: 6031012-6030161 (Counterclockwise)

Preceding gene: 170752073

Following gene: 170752071

Centisome position: 99.23

GC content: 70.54

Gene sequence:

>852_bases
GTGGTTTCGGGCTCACGTCGTGTGGGCCGCCAGCGGGCCCGGAATAAAATCCAGACTGAGACGAAGGCAGCCAGCGCCGT
CCGGGCGCTTGGGTACCTGCGGGTCTCGACGGAAGAGCAGGCCGCGACCGGCCACGGGCTTGAGGCGCAGGAGAAGGCGG
TCCGCTCCTTCGCCGAGAGCCAGGGCTACGAGCTGATCGAGGTTCTCACCGACCCGGGCGTCTCGGGCGCGACCCGGCCC
GCCGATCGCCCCGCCTTCGGGCAGGCGCTTGAGCTCGCTGCGGCCAAGGCCTTCGACGTGCTGCTGGTGGCGAAGATAGA
CCGGCTGTCCCGCGACATCCGCCACGCGATGACGACGGTCTCGGATCTGGCAGAGCAGCACGAGGTCGCCTTCCGGTCGG
TCACGGAATCGGTGATCGACACCTCGAACCCGATGGGGCGGACCTTCTTCGCCATCTTCGCCGGCATGGCGGAGAACGAG
CGGTTCGTGATCCGCGACCGGACCGCCGGCGGCCGGGTGGCCAAGGCCGGGAAGGGCGGCTTCGCCGGGGGGCAGGCGCC
CTACGGCTACACGAAGGATCTCGAGGGCGGCCTCCGGGTCGTCCCGGAGCAGGCGCGCATCGTCCGCCGGATCTACCAGG
AGCGCCGCCGGAAGCGGACGCTCCAGGCCATCGCCGACGGCTTGAACGCCGAGGGCATTCCGGCGCCGAAGGGCGGCCGC
TGGGCCGTCTCCACGGTCGCCTACGTGACCGACAACCCGAAATATCGCGGCGCGATCGAGTACCTGTTCCGCTGGAACGG
GACCGAGACGCACGTCCTGCAGCCGGGCGCCCACGCCGCGATCATCGGCTGA

Upstream 100 bases:

>100_bases
GTCGGGCAGCCTGATCGGAGGCCATTTCCAAGGTCTCCTGAGATCTTGGAAATATCGGCCGTTTAGACGCTAAAACGTAA
GCAAAATCAGAGGCTTGCAG

Downstream 100 bases:

>100_bases
CGAAGGCTTCCATGCGCATCGAACTCGTCCACGAGCCCTCCGCCGGGCCTCGTCCCTTCCTGCTGCGCGCTCGTCTCGCC
GGCGGCCTCGTCCAGGAGGG

Product: resolvase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MVSGSRRVGRQRARNKIQTETKAASAVRALGYLRVSTEEQAATGHGLEAQEKAVRSFAESQGYELIEVLTDPGVSGATRP
ADRPAFGQALELAAAKAFDVLLVAKIDRLSRDIRHAMTTVSDLAEQHEVAFRSVTESVIDTSNPMGRTFFAIFAGMAENE
RFVIRDRTAGGRVAKAGKGGFAGGQAPYGYTKDLEGGLRVVPEQARIVRRIYQERRRKRTLQAIADGLNAEGIPAPKGGR
WAVSTVAYVTDNPKYRGAIEYLFRWNGTETHVLQPGAHAAIIG

Sequences:

>Translated_283_residues
MVSGSRRVGRQRARNKIQTETKAASAVRALGYLRVSTEEQAATGHGLEAQEKAVRSFAESQGYELIEVLTDPGVSGATRP
ADRPAFGQALELAAAKAFDVLLVAKIDRLSRDIRHAMTTVSDLAEQHEVAFRSVTESVIDTSNPMGRTFFAIFAGMAENE
RFVIRDRTAGGRVAKAGKGGFAGGQAPYGYTKDLEGGLRVVPEQARIVRRIYQERRRKRTLQAIADGLNAEGIPAPKGGR
WAVSTVAYVTDNPKYRGAIEYLFRWNGTETHVLQPGAHAAIIG
>Mature_283_residues
MVSGSRRVGRQRARNKIQTETKAASAVRALGYLRVSTEEQAATGHGLEAQEKAVRSFAESQGYELIEVLTDPGVSGATRP
ADRPAFGQALELAAAKAFDVLLVAKIDRLSRDIRHAMTTVSDLAEQHEVAFRSVTESVIDTSNPMGRTFFAIFAGMAENE
RFVIRDRTAGGRVAKAGKGGFAGGQAPYGYTKDLEGGLRVVPEQARIVRRIYQERRRKRTLQAIADGLNAEGIPAPKGGR
WAVSTVAYVTDNPKYRGAIEYLFRWNGTETHVLQPGAHAAIIG

Specific function: This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=158, Percent_Identity=37.9746835443038, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 30725; Mature: 30725

Theoretical pI: Translated: 10.43; Mature: 10.43

Prosite motif: PS00397 RECOMBINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSGSRRVGRQRARNKIQTETKAASAVRALGYLRVSTEEQAATGHGLEAQEKAVRSFAES
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHCCCCCHHHHHHHHHHHHH
QGYELIEVLTDPGVSGATRPADRPAFGQALELAAAKAFDVLLVAKIDRLSRDIRHAMTTV
CCCEEEEHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SDLAEQHEVAFRSVTESVIDTSNPMGRTFFAIFAGMAENERFVIRDRTAGGRVAKAGKGG
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCEEECCCCC
FAGGQAPYGYTKDLEGGLRVVPEQARIVRRIYQERRRKRTLQAIADGLNAEGIPAPKGGR
CCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
WAVSTVAYVTDNPKYRGAIEYLFRWNGTETHVLQPGAHAAIIG
EEEEEEEEEECCCCHHHHHHHHEECCCCCCEEECCCCCEECCC
>Mature Secondary Structure
MVSGSRRVGRQRARNKIQTETKAASAVRALGYLRVSTEEQAATGHGLEAQEKAVRSFAES
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHCCCCCHHHHHHHHHHHHH
QGYELIEVLTDPGVSGATRPADRPAFGQALELAAAKAFDVLLVAKIDRLSRDIRHAMTTV
CCCEEEEHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SDLAEQHEVAFRSVTESVIDTSNPMGRTFFAIFAGMAENERFVIRDRTAGGRVAKAGKGG
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCEEECCCCC
FAGGQAPYGYTKDLEGGLRVVPEQARIVRRIYQERRRKRTLQAIADGLNAEGIPAPKGGR
CCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
WAVSTVAYVTDNPKYRGAIEYLFRWNGTETHVLQPGAHAAIIG
EEEEEEEEEECCCCHHHHHHHHEECCCCCCEEECCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3894006; 6310572; 8905232; 9278503 [H]