Click here to switch to the map view.

The map label for this gene is rpoH [H]

Identifier: 170752008

GI number: 170752008

Start: 5970478

End: 5971371

Strand: Reverse

Name: rpoH [H]

Synonym: Mrad2831_5640

Alternate gene names: 170752008

Gene position: 5971371-5970478 (Counterclockwise)

Preceding gene: 170752009

Following gene: 170751998

Centisome position: 98.25

GC content: 66.89

Gene sequence:

>894_bases
ATGGCCGGTACGCTGCCCGTGCTCGCCAACGAAGGTGGCCTCTCGCGCTACCTCGACGAGATTCGCAAGTTCCCGATGCT
GGAGCCGTCGGAGGAGTTCACGCTGGCGAAGGCTTGGCGGGACGCCGGCGACCGCGAGGCCGCGCACCGGCTCGTGACGT
CGCATCTCCGCCTCGTGGCCAAGATTGCCATGGGTTACCGCGGCTACGGCCTGCCGATCGGCGAGGTCGTGTCGGAGGGC
AATGTCGGTCTCATGCAGGCGGTCAAGCGCTTCGATCCCGACAAGGGCTTCCGTTTGGCCACCTACGCAATGTGGTGGAT
CAAGGCGGCGATCCAGGAATACATCCTGCGCTCGTGGTCCCTCGTGAAGATGGGCACGACCGCGAACCAGAAGAAGCTGT
TCTTCAATCTGCGGAAGGCGAAGGGCAAGATCTCGGCCCTCGACGAGGGCGATCTGAAGCCCGATCAGGTGAAGCAGATC
GCCACCCGCCTCGGTGTGCCTGAGCAGGACGTGATCGACATGAACCGGCGCCTGTCCGGCGACACGTCGCTCAACGCGCC
CCTGCGCGAGGAGGGCGAGGGCGAGTGGCAGGACTGGCTCGTGGACAACAGCCCGAGCCAGGAGACCGTGCTGGCGCGGG
AGCAGGAGGGCCAGAACCGGCTCTCCGCGCTCAAGGACGCTCTCGGCGTACTGAACCCGCGCGAGCGCCGCATCTTCGAG
GCCCGGCGTCTCGCCGAGGACCCGATCACTCTCGAGGATCTCTCCGGCGAGTTCGGCGTCTCCCGTGAGCGCGTCCGGCA
GATCGAGGTTCGCGCCTTCGAGAAGGTGCAGGAGGCGGTCAAGCGCAACATCGCCACCCGCGAGTTGCCCCGGACCGGCA
TCGAGGCCCGCTGA

Upstream 100 bases:

>100_bases
ATGGGGGGCCGGCAGGGTCGGCTCAAGAGCGGCCCGAGTGGACGGCACTGTTGTCCGTCGCGTCCGCCCGACAGGGGGAC
ATTAGGTCCAGGAGAATGCC

Downstream 100 bases:

>100_bases
CCCGCACGCCCGGCGCTCGCGAGGCGCCGGGCTCGCCCAGCCCGGTCAGGGCTCGGCGGTCAGGGCTCGAACGAATGCCG
ACGCCGCGCGGTCACTGCCA

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MAGTLPVLANEGGLSRYLDEIRKFPMLEPSEEFTLAKAWRDAGDREAAHRLVTSHLRLVAKIAMGYRGYGLPIGEVVSEG
NVGLMQAVKRFDPDKGFRLATYAMWWIKAAIQEYILRSWSLVKMGTTANQKKLFFNLRKAKGKISALDEGDLKPDQVKQI
ATRLGVPEQDVIDMNRRLSGDTSLNAPLREEGEGEWQDWLVDNSPSQETVLAREQEGQNRLSALKDALGVLNPRERRIFE
ARRLAEDPITLEDLSGEFGVSRERVRQIEVRAFEKVQEAVKRNIATRELPRTGIEAR

Sequences:

>Translated_297_residues
MAGTLPVLANEGGLSRYLDEIRKFPMLEPSEEFTLAKAWRDAGDREAAHRLVTSHLRLVAKIAMGYRGYGLPIGEVVSEG
NVGLMQAVKRFDPDKGFRLATYAMWWIKAAIQEYILRSWSLVKMGTTANQKKLFFNLRKAKGKISALDEGDLKPDQVKQI
ATRLGVPEQDVIDMNRRLSGDTSLNAPLREEGEGEWQDWLVDNSPSQETVLAREQEGQNRLSALKDALGVLNPRERRIFE
ARRLAEDPITLEDLSGEFGVSRERVRQIEVRAFEKVQEAVKRNIATRELPRTGIEAR
>Mature_296_residues
AGTLPVLANEGGLSRYLDEIRKFPMLEPSEEFTLAKAWRDAGDREAAHRLVTSHLRLVAKIAMGYRGYGLPIGEVVSEGN
VGLMQAVKRFDPDKGFRLATYAMWWIKAAIQEYILRSWSLVKMGTTANQKKLFFNLRKAKGKISALDEGDLKPDQVKQIA
TRLGVPEQDVIDMNRRLSGDTSLNAPLREEGEGEWQDWLVDNSPSQETVLAREQEGQNRLSALKDALGVLNPRERRIFEA
RRLAEDPITLEDLSGEFGVSRERVRQIEVRAFEKVQEAVKRNIATRELPRTGIEAR

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=290, Percent_Identity=39.6551724137931, Blast_Score=187, Evalue=5e-49,
Organism=Escherichia coli, GI1789098, Length=272, Percent_Identity=36.3970588235294, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1789448, Length=248, Percent_Identity=28.6290322580645, Blast_Score=91, Evalue=6e-20,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 33587; Mature: 33456

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGTLPVLANEGGLSRYLDEIRKFPMLEPSEEFTLAKAWRDAGDREAAHRLVTSHLRLVA
CCCCCCCEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
KIAMGYRGYGLPIGEVVSEGNVGLMQAVKRFDPDKGFRLATYAMWWIKAAIQEYILRSWS
HHHHCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
LVKMGTTANQKKLFFNLRKAKGKISALDEGDLKPDQVKQIATRLGVPEQDVIDMNRRLSG
CEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCC
DTSLNAPLREEGEGEWQDWLVDNSPSQETVLAREQEGQNRLSALKDALGVLNPRERRIFE
CCCCCCCCHHCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
ARRLAEDPITLEDLSGEFGVSRERVRQIEVRAFEKVQEAVKRNIATRELPRTGIEAR
HHHHHCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AGTLPVLANEGGLSRYLDEIRKFPMLEPSEEFTLAKAWRDAGDREAAHRLVTSHLRLVA
CCCCCCEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
KIAMGYRGYGLPIGEVVSEGNVGLMQAVKRFDPDKGFRLATYAMWWIKAAIQEYILRSWS
HHHHCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
LVKMGTTANQKKLFFNLRKAKGKISALDEGDLKPDQVKQIATRLGVPEQDVIDMNRRLSG
CEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCC
DTSLNAPLREEGEGEWQDWLVDNSPSQETVLAREQEGQNRLSALKDALGVLNPRERRIFE
CCCCCCCCHHCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
ARRLAEDPITLEDLSGEFGVSRERVRQIEVRAFEKVQEAVKRNIATRELPRTGIEAR
HHHHHCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7501460 [H]