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The map label for this gene is ytcI [H]

Identifier: 170751826

GI number: 170751826

Start: 5767445

End: 5768986

Strand: Reverse

Name: ytcI [H]

Synonym: Mrad2831_5458

Alternate gene names: 170751826

Gene position: 5768986-5767445 (Counterclockwise)

Preceding gene: 170751827

Following gene: 170751824

Centisome position: 94.92

GC content: 73.87

Gene sequence:

>1542_bases
GTGTCCGACAGCCAGCACGATGATCGCCGTCCGCCGGCCCGCTTCAACGCGGCGCGGTACTGCCTCGCCGAGAACGCGCG
CCTGCGCCCCGACAAGACCGCCCTGGTGATGGTCGGCGACGGCACCGCGGACCGCCTCAGCTTCGGCGCGGTCGAGCGCG
CGGTGCGCGGCATCGCGGCGGGCCTCCTCGGCCTCGGCCTGAAGCCCGGCGACCGGGTGATGATCCGCATGGGCAACGAG
GCCGACTACGTCCTCGTCTACTTCGGTGCCCTGGCGGCCGGCCTCGTGGCGCTGCCCTCCTCGCCGCAGCTCACGGAGGG
CGAGGCCGCCTTCCTGATGGAGAATTCCGGCGCCGCCGCCGTGGTCGTCGGGAGCGGCTGCACGGTCGCGCCCGAGGCTC
TGGGCGGGCGCCTCCTGCTCGACGCCGCCGCGGTCGCCGCCCTGAAGGCGGGCCCGCCCCTGCCCGACTACGCCGACACC
GCCGCCGACGACCCGGCGATGCTGATCTACACCTCCGGCACGACGAGCCGGCCGAAGGGCGTGCTGCACGCCCACCGCGC
CGCCTACGGCCGCCGCCCGATGCTCGACCACTGGCTCGGGCTCACCGAATCCGACGTGATGCTCCACGCCGGCACCATGA
ACTGGACCTACACGCTGGGCGTCGGCCTGAGCGATCCCTGGTCGGTCGGCGCGACCGCGGTGCTCTACAACGGCCCGCGC
GATCCCGGGGTGTGGCCGCGCCTGATCGCCGCCTACGGCGCGACCCTGTTCGCCGCCGTCCCGACGCTCTACCGCCAGAT
CCTCAAGTACGGCGACCTCGGCGCCCACGACCTGTCGAGCCTGCGCCACGGCTGCACGGCCGGCGAGCCCCTCGCGGTGG
AGCTTCTGGAGGCCTGGGGTCAGGCCACCGGCAAGCCCCTCTACGAGGCACTCGGGATGAGCGAGATCTCGACCTACATC
TCCAGCGGCCCGACCATCCCGGTGCGCCCCGGCTCGCCCGGTAAGCCGCAGCCGGGCCGGCGCGTGGCGATCCTGCCCGT
CGCGGGCGAGCCCGTGCCGCTTCCGGCCGGCGAGAGCGGGCTCCTCGCCGTCCACCGGACCGAGCCGGCGCTGATGCTCG
GCTACTGGAACCGGCCGGAAGAAGAGGCCGAGGTCATGCGCGGCGACTGGTTCGTCGGCGGCGACCTCGCCCGTCTCGAC
GCCGACGGCTACCTGTGGTTCGAGGGCCGCAACAACGACCTGATGAAGGCGATGGGCTACCGGGTCTCGCCCAACGAGGT
GGAGGCGGTGCTGTCGACCCACCCGTCCGTGGCGGAGGTGGGCGTCGCCGAGCTGCCGGTGCGTGCGGACGTCTCGGTCA
TCTGCGGCTTCGTGGTGCTCCAGCCGGGCGTCGAGCCCGACGCGGAGGGCCTGCTCGCGTGGTGTGCCGAGCGGCTCGCC
GCCTACAAGCGCCCGCGCGAGATCCGCTTCCTGGATGCCCTGCCGCGCACCGCGAACGGCAAGGTTCAGCGGAAGCGGCT
GGCCGAGGCGGTGGCGGGCTGA

Upstream 100 bases:

>100_bases
CGCCGGCGGACCTCGCGCCGTGCGCGGCGGCGGCCCCTGTTGACGCAGGCGCGCCCAGGCGGGACACCCGGCGAAAAGCC
CGCCCGCGGGGGAGCGCCCG

Downstream 100 bases:

>100_bases
AGCAGGCTCGAACGCCATCCGGACGGGAGGCGGGTCCCCGCTCCCGCACGGGATAAGGGGCCGGTCGCACGCGGTCATCC
ACGGCATTGGCCGGGCTGCG

Product: AMP-dependent synthetase and ligase

Products: AMP; diphosphate; acetyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 513; Mature: 512

Protein sequence:

>513_residues
MSDSQHDDRRPPARFNAARYCLAENARLRPDKTALVMVGDGTADRLSFGAVERAVRGIAAGLLGLGLKPGDRVMIRMGNE
ADYVLVYFGALAAGLVALPSSPQLTEGEAAFLMENSGAAAVVVGSGCTVAPEALGGRLLLDAAAVAALKAGPPLPDYADT
AADDPAMLIYTSGTTSRPKGVLHAHRAAYGRRPMLDHWLGLTESDVMLHAGTMNWTYTLGVGLSDPWSVGATAVLYNGPR
DPGVWPRLIAAYGATLFAAVPTLYRQILKYGDLGAHDLSSLRHGCTAGEPLAVELLEAWGQATGKPLYEALGMSEISTYI
SSGPTIPVRPGSPGKPQPGRRVAILPVAGEPVPLPAGESGLLAVHRTEPALMLGYWNRPEEEAEVMRGDWFVGGDLARLD
ADGYLWFEGRNNDLMKAMGYRVSPNEVEAVLSTHPSVAEVGVAELPVRADVSVICGFVVLQPGVEPDAEGLLAWCAERLA
AYKRPREIRFLDALPRTANGKVQRKRLAEAVAG

Sequences:

>Translated_513_residues
MSDSQHDDRRPPARFNAARYCLAENARLRPDKTALVMVGDGTADRLSFGAVERAVRGIAAGLLGLGLKPGDRVMIRMGNE
ADYVLVYFGALAAGLVALPSSPQLTEGEAAFLMENSGAAAVVVGSGCTVAPEALGGRLLLDAAAVAALKAGPPLPDYADT
AADDPAMLIYTSGTTSRPKGVLHAHRAAYGRRPMLDHWLGLTESDVMLHAGTMNWTYTLGVGLSDPWSVGATAVLYNGPR
DPGVWPRLIAAYGATLFAAVPTLYRQILKYGDLGAHDLSSLRHGCTAGEPLAVELLEAWGQATGKPLYEALGMSEISTYI
SSGPTIPVRPGSPGKPQPGRRVAILPVAGEPVPLPAGESGLLAVHRTEPALMLGYWNRPEEEAEVMRGDWFVGGDLARLD
ADGYLWFEGRNNDLMKAMGYRVSPNEVEAVLSTHPSVAEVGVAELPVRADVSVICGFVVLQPGVEPDAEGLLAWCAERLA
AYKRPREIRFLDALPRTANGKVQRKRLAEAVAG
>Mature_512_residues
SDSQHDDRRPPARFNAARYCLAENARLRPDKTALVMVGDGTADRLSFGAVERAVRGIAAGLLGLGLKPGDRVMIRMGNEA
DYVLVYFGALAAGLVALPSSPQLTEGEAAFLMENSGAAAVVVGSGCTVAPEALGGRLLLDAAAVAALKAGPPLPDYADTA
ADDPAMLIYTSGTTSRPKGVLHAHRAAYGRRPMLDHWLGLTESDVMLHAGTMNWTYTLGVGLSDPWSVGATAVLYNGPRD
PGVWPRLIAAYGATLFAAVPTLYRQILKYGDLGAHDLSSLRHGCTAGEPLAVELLEAWGQATGKPLYEALGMSEISTYIS
SGPTIPVRPGSPGKPQPGRRVAILPVAGEPVPLPAGESGLLAVHRTEPALMLGYWNRPEEEAEVMRGDWFVGGDLARLDA
DGYLWFEGRNNDLMKAMGYRVSPNEVEAVLSTHPSVAEVGVAELPVRADVSVICGFVVLQPGVEPDAEGLLAWCAERLAA
YKRPREIRFLDALPRTANGKVQRKRLAEAVAG

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI42544132, Length=532, Percent_Identity=29.5112781954887, Blast_Score=230, Evalue=2e-60,
Organism=Homo sapiens, GI38505220, Length=536, Percent_Identity=32.2761194029851, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI58082049, Length=536, Percent_Identity=29.6641791044776, Blast_Score=202, Evalue=9e-52,
Organism=Homo sapiens, GI122937307, Length=534, Percent_Identity=29.4007490636704, Blast_Score=199, Evalue=6e-51,
Organism=Homo sapiens, GI157311624, Length=536, Percent_Identity=28.9179104477612, Blast_Score=192, Evalue=5e-49,
Organism=Homo sapiens, GI157311622, Length=536, Percent_Identity=28.9179104477612, Blast_Score=192, Evalue=5e-49,
Organism=Homo sapiens, GI115511026, Length=542, Percent_Identity=27.859778597786, Blast_Score=189, Evalue=7e-48,
Organism=Homo sapiens, GI187761345, Length=487, Percent_Identity=29.3634496919918, Blast_Score=162, Evalue=8e-40,
Organism=Homo sapiens, GI187761343, Length=487, Percent_Identity=29.3634496919918, Blast_Score=162, Evalue=8e-40,
Organism=Homo sapiens, GI156151445, Length=551, Percent_Identity=26.8602540834846, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI195947378, Length=442, Percent_Identity=26.6968325791855, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI42544134, Length=363, Percent_Identity=26.4462809917355, Blast_Score=129, Evalue=8e-30,
Organism=Homo sapiens, GI253970489, Length=376, Percent_Identity=29.5212765957447, Blast_Score=109, Evalue=7e-24,
Organism=Homo sapiens, GI8923896, Length=376, Percent_Identity=29.5212765957447, Blast_Score=109, Evalue=7e-24,
Organism=Homo sapiens, GI28416953, Length=566, Percent_Identity=25.2650176678445, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI38524616, Length=528, Percent_Identity=25.3787878787879, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI13375727, Length=387, Percent_Identity=27.1317829457364, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI13325057, Length=374, Percent_Identity=28.0748663101604, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1788107, Length=553, Percent_Identity=28.2097649186257, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1790505, Length=557, Percent_Identity=26.032315978456, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI221142682, Length=511, Percent_Identity=25.8317025440313, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI145693145, Length=518, Percent_Identity=24.7104247104247, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1786529, Length=578, Percent_Identity=26.2975778546713, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1789201, Length=352, Percent_Identity=25.5681818181818, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1786810, Length=510, Percent_Identity=26.4705882352941, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786801, Length=524, Percent_Identity=25.5725190839695, Blast_Score=97, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17558820, Length=507, Percent_Identity=26.232741617357, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI32563687, Length=498, Percent_Identity=25.9036144578313, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71994694, Length=538, Percent_Identity=23.0483271375465, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI71994690, Length=538, Percent_Identity=23.0483271375465, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI71994703, Length=538, Percent_Identity=23.0483271375465, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17560308, Length=566, Percent_Identity=24.3816254416961, Blast_Score=112, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17557194, Length=529, Percent_Identity=25.1417769376182, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17531443, Length=523, Percent_Identity=24.0917782026769, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71982997, Length=579, Percent_Identity=26.7702936096718, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI71983001, Length=579, Percent_Identity=26.7702936096718, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17538037, Length=525, Percent_Identity=24.5714285714286, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17559526, Length=545, Percent_Identity=22.7522935779817, Blast_Score=98, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17560140, Length=523, Percent_Identity=21.9885277246654, Blast_Score=83, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323182, Length=568, Percent_Identity=26.5845070422535, Blast_Score=155, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6319699, Length=489, Percent_Identity=26.1758691206544, Blast_Score=134, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6319264, Length=571, Percent_Identity=25.2189141856392, Blast_Score=133, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24667959, Length=376, Percent_Identity=29.5212765957447, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI62472339, Length=376, Percent_Identity=29.5212765957447, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24667955, Length=376, Percent_Identity=29.5212765957447, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24648260, Length=515, Percent_Identity=25.8252427184466, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24581924, Length=528, Percent_Identity=24.2424242424242, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI18859661, Length=514, Percent_Identity=26.0700389105058, Blast_Score=115, Evalue=8e-26,
Organism=Drosophila melanogaster, GI19922652, Length=512, Percent_Identity=24.0234375, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI281365686, Length=356, Percent_Identity=22.1910112359551, Blast_Score=100, Evalue=5e-21,
Organism=Drosophila melanogaster, GI21355181, Length=538, Percent_Identity=23.9776951672862, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21355225, Length=546, Percent_Identity=24.1758241758242, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI21356441, Length=353, Percent_Identity=25.4957507082153, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24648253, Length=375, Percent_Identity=25.0666666666667, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24648255, Length=375, Percent_Identity=25.3333333333333, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24656500, Length=529, Percent_Identity=23.4404536862004, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648257, Length=370, Percent_Identity=25.1351351351351, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI20130357, Length=354, Percent_Identity=23.4463276836158, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI21358303, Length=356, Percent_Identity=22.752808988764, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24653035, Length=356, Percent_Identity=22.1910112359551, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21356947, Length=342, Percent_Identity=23.0994152046784, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI161076582, Length=369, Percent_Identity=22.4932249322493, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.1

Molecular weight: Translated: 54385; Mature: 54254

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSQHDDRRPPARFNAARYCLAENARLRPDKTALVMVGDGTADRLSFGAVERAVRGIAA
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHH
GLLGLGLKPGDRVMIRMGNEADYVLVYFGALAAGLVALPSSPQLTEGEAAFLMENSGAAA
HHHHCCCCCCCEEEEEECCCCCEEEEEHHHHHHHHEECCCCCCCCCCCEEEEEECCCCEE
VVVGSGCTVAPEALGGRLLLDAAAVAALKAGPPLPDYADTAADDPAMLIYTSGTTSRPKG
EEECCCCEECHHHCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCH
VLHAHRAAYGRRPMLDHWLGLTESDVMLHAGTMNWTYTLGVGLSDPWSVGATAVLYNGPR
HHHHHHHHHCCCCHHHHHCCCCCCCEEEEECCCCEEEEEECCCCCCCCCCCEEEEECCCC
DPGVWPRLIAAYGATLFAAVPTLYRQILKYGDLGAHDLSSLRHGCTAGEPLAVELLEAWG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
QATGKPLYEALGMSEISTYISSGPTIPVRPGSPGKPQPGRRVAILPVAGEPVPLPAGESG
CCCCCHHHHHHCHHHHHHHHHCCCEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCCC
LLAVHRTEPALMLGYWNRPEEEAEVMRGDWFVGGDLARLDADGYLWFEGRNNDLMKAMGY
EEEEEECCCEEEEEECCCCHHHHHHHCCCEEECCCEEEECCCCEEEEECCCCCHHHHCCC
RVSPNEVEAVLSTHPSVAEVGVAELPVRADVSVICGFVVLQPGVEPDAEGLLAWCAERLA
CCCHHHHHHHHHCCCCHHHCCHHHCCCCCCHHHHHHHHEECCCCCCCHHHHHHHHHHHHH
AYKRPREIRFLDALPRTANGKVQRKRLAEAVAG
HHCCCCCEEHHHHCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SDSQHDDRRPPARFNAARYCLAENARLRPDKTALVMVGDGTADRLSFGAVERAVRGIAA
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHH
GLLGLGLKPGDRVMIRMGNEADYVLVYFGALAAGLVALPSSPQLTEGEAAFLMENSGAAA
HHHHCCCCCCCEEEEEECCCCCEEEEEHHHHHHHHEECCCCCCCCCCCEEEEEECCCCEE
VVVGSGCTVAPEALGGRLLLDAAAVAALKAGPPLPDYADTAADDPAMLIYTSGTTSRPKG
EEECCCCEECHHHCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCH
VLHAHRAAYGRRPMLDHWLGLTESDVMLHAGTMNWTYTLGVGLSDPWSVGATAVLYNGPR
HHHHHHHHHCCCCHHHHHCCCCCCCEEEEECCCCEEEEEECCCCCCCCCCCEEEEECCCC
DPGVWPRLIAAYGATLFAAVPTLYRQILKYGDLGAHDLSSLRHGCTAGEPLAVELLEAWG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
QATGKPLYEALGMSEISTYISSGPTIPVRPGSPGKPQPGRRVAILPVAGEPVPLPAGESG
CCCCCHHHHHHCHHHHHHHHHCCCEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCCC
LLAVHRTEPALMLGYWNRPEEEAEVMRGDWFVGGDLARLDADGYLWFEGRNNDLMKAMGY
EEEEEECCCEEEEEECCCCHHHHHHHCCCEEECCCEEEECCCCEEEEECCCCCHHHHCCC
RVSPNEVEAVLSTHPSVAEVGVAELPVRADVSVICGFVVLQPGVEPDAEGLLAWCAERLA
CCCHHHHHHHHHCCCCHHHCCHHHCCCCCCHHHHHHHHEECCCCCCCHHHHHHHHHHHHH
AYKRPREIRFLDALPRTANGKVQRKRLAEAVAG
HHCCCCCEEHHHHCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; acetate; CoA

Specific reaction: ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]