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The map label for this gene is est [H]

Identifier: 170751820

GI number: 170751820

Start: 5760316

End: 5761218

Strand: Reverse

Name: est [H]

Synonym: Mrad2831_5450

Alternate gene names: 170751820

Gene position: 5761218-5760316 (Counterclockwise)

Preceding gene: 170751821

Following gene: 170751819

Centisome position: 94.79

GC content: 75.53

Gene sequence:

>903_bases
ATGGCGAGCCTGCGCGCGTATCTCGGCGCCTACATGGTCCGCAGCCGGGTGCGGGGCCCCCTGTCCCGGGCGCGGGACGC
GGCCGCGTTCCGCCGGATCTTCGAGGCGCCGACCTTCCCCGACCCCAAGGGCGTCACCTACGAGCCGGGCATCGTCGGCG
GCGTGAAGGGCGAGTGGGTTCGGCCGAAGGCCGGGCCCGGCCGGCGGATGCTCTACATCCACGGCGGCGGCTTCATCGCG
TGCTCGCCGCGCACGCACCGTCCCATCACCGGCGCGCTGGCGAACCGGGGCTTCACGATCTTCGCGCCCGACTACCGCCT
CGCCCCCGAGCACCCGTTCCCCGCGGCGGTCGACGACGTGACCGCCGCCTGGGCGGCGTTCAGCGCCGAGGGCGCCGCCT
GCGTGGCGGGCGAGTCGGCCGGCGGCAACCTCGCCCTCGTGCTGATGGGCCAAGCCCGCGCCCGCGGCCTGCCGATGCCC
TGGGCGGCGGCCCTGTTCTCGCCGGCCACGGACTTCGTCTCCGAGGACGGGTCGCGGGTGACCAATGCGTGGCGCGACGC
CATGTTTGACCCGCAGGCGCTCGCGGCGATCCGGCCGATGTATCTGGGCGCCGCCGATCCGGCCGATCCGCGGGTCTCGC
CGCTCTTCGGCGACCCGACCGGCTTCCCGCCGCTGCTGTTCCATGTCGGCGCCCGCGAGGTCCTGCGCGACGACTCCGTG
CGCATGGCGGAGCGGGCCCGCGCGGCCGGCGTCGAGACCGAGCTCAAGCTCTTCCCGGTCGTGTCCCACGCCTGGCAGTT
CGCCGCCGGCGTGCTGCCCGAGGCGCGAACCTCCCTCGACGAGGCCGCGGCCTTCCTGACGGCGCACGCGCCCCACGCCC
CCTTCGGGAGCCCTCAGCGATGA

Upstream 100 bases:

>100_bases
GTCGTCGGCAAGGACGCCAAGAACGCCGCGCTGATCCAGCGCCTGATGCCGGTGGGCTACTGGAAGACGATCGTGCGCCT
GTCCGGAGGGACGCTCTGAG

Downstream 100 bases:

>100_bases
CCGAGCCCGCGTCCGAGACCCTGGCCGTCCTGATCGTCGGCGCCGGCTTCTCGGGGCTCGCGATGGCGATCCGCTGCCGG
CAGGCGGGAATCGGGCCGCT

Product: alpha/beta hydrolase domain-containing protein

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MASLRAYLGAYMVRSRVRGPLSRARDAAAFRRIFEAPTFPDPKGVTYEPGIVGGVKGEWVRPKAGPGRRMLYIHGGGFIA
CSPRTHRPITGALANRGFTIFAPDYRLAPEHPFPAAVDDVTAAWAAFSAEGAACVAGESAGGNLALVLMGQARARGLPMP
WAAALFSPATDFVSEDGSRVTNAWRDAMFDPQALAAIRPMYLGAADPADPRVSPLFGDPTGFPPLLFHVGAREVLRDDSV
RMAERARAAGVETELKLFPVVSHAWQFAAGVLPEARTSLDEAAAFLTAHAPHAPFGSPQR

Sequences:

>Translated_300_residues
MASLRAYLGAYMVRSRVRGPLSRARDAAAFRRIFEAPTFPDPKGVTYEPGIVGGVKGEWVRPKAGPGRRMLYIHGGGFIA
CSPRTHRPITGALANRGFTIFAPDYRLAPEHPFPAAVDDVTAAWAAFSAEGAACVAGESAGGNLALVLMGQARARGLPMP
WAAALFSPATDFVSEDGSRVTNAWRDAMFDPQALAAIRPMYLGAADPADPRVSPLFGDPTGFPPLLFHVGAREVLRDDSV
RMAERARAAGVETELKLFPVVSHAWQFAAGVLPEARTSLDEAAAFLTAHAPHAPFGSPQR
>Mature_299_residues
ASLRAYLGAYMVRSRVRGPLSRARDAAAFRRIFEAPTFPDPKGVTYEPGIVGGVKGEWVRPKAGPGRRMLYIHGGGFIAC
SPRTHRPITGALANRGFTIFAPDYRLAPEHPFPAAVDDVTAAWAAFSAEGAACVAGESAGGNLALVLMGQARARGLPMPW
AAALFSPATDFVSEDGSRVTNAWRDAMFDPQALAAIRPMYLGAADPADPRVSPLFGDPTGFPPLLFHVGAREVLRDDSVR
MAERARAAGVETELKLFPVVSHAWQFAAGVLPEARTSLDEAAAFLTAHAPHAPFGSPQR

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Secreted (Potential). Note=External to the cytoplasmic membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21328446, Length=123, Percent_Identity=38.2113821138211, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1786682, Length=286, Percent_Identity=27.2727272727273, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 31959; Mature: 31828

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS01173 LIPASE_GDXG_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASLRAYLGAYMVRSRVRGPLSRARDAAAFRRIFEAPTFPDPKGVTYEPGIVGGVKGEWV
CCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCEECCCCCEE
RPKAGPGRRMLYIHGGGFIACSPRTHRPITGALANRGFTIFAPDYRLAPEHPFPAAVDDV
CCCCCCCCEEEEEECCCEEEECCCCCCCCHHHHHCCCEEEECCCCCCCCCCCCCCHHHHH
TAAWAAFSAEGAACVAGESAGGNLALVLMGQARARGLPMPWAAALFSPATDFVSEDGSRV
HHHHHHHCCCCCEEEECCCCCCCEEEEEEECHHHCCCCCHHHHHHHCCHHHHHHCCCHHH
TNAWRDAMFDPQALAAIRPMYLGAADPADPRVSPLFGDPTGFPPLLFHVGAREVLRDDSV
HHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHCHHHHHHCCHH
RMAERARAAGVETELKLFPVVSHAWQFAAGVLPEARTSLDEAAAFLTAHAPHAPFGSPQR
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
ASLRAYLGAYMVRSRVRGPLSRARDAAAFRRIFEAPTFPDPKGVTYEPGIVGGVKGEWV
CHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCEECCCCCEE
RPKAGPGRRMLYIHGGGFIACSPRTHRPITGALANRGFTIFAPDYRLAPEHPFPAAVDDV
CCCCCCCCEEEEEECCCEEEECCCCCCCCHHHHHCCCEEEECCCCCCCCCCCCCCHHHHH
TAAWAAFSAEGAACVAGESAGGNLALVLMGQARARGLPMPWAAALFSPATDFVSEDGSRV
HHHHHHHCCCCCEEEECCCCCCCEEEEEEECHHHCCCCCHHHHHHHCCHHHHHHCCCHHH
TNAWRDAMFDPQALAAIRPMYLGAADPADPRVSPLFGDPTGFPPLLFHVGAREVLRDDSV
HHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHCHHHHHHCCHH
RMAERARAAGVETELKLFPVVSHAWQFAAGVLPEARTSLDEAAAFLTAHAPHAPFGSPQR
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2545531 [H]