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The map label for this gene is prfB [H]

Identifier: 170751458

GI number: 170751458

Start: 5393163

End: 5394128

Strand: Direct

Name: prfB [H]

Synonym: Mrad2831_5077

Alternate gene names: 170751458

Gene position: 5393163-5394128 (Clockwise)

Preceding gene: 170751456

Following gene: 170751465

Centisome position: 88.73

GC content: 68.01

Gene sequence:

>966_bases
ATGCGCGAGCGCCAGCAGCTCGACGAGGCCGTCACGGCGATCAAGAAGCTCGAGCGCGACCTCGAGGACGGCGCCACGCT
GATCGAACTCGGCGAGATGGAGGGTGACGAATCCTCGATCCGCGAGGGCGAGGCGGCGATCCTGGCCGTCGAGAAGGAGG
CCGCGAGCCGGCAGGTCGAGACCCTACTGTCCGGCGAGGCCGACGGCTTCGACACCTATCTCGAAGTGCATGCGGGCGCC
GGCGGCACCGAGAGCCAGGACTGGGCCAACATGCTCCAGCGCATGTACGCCCGCTGGGCCGAGCGCCGGAAGTACAAGGT
CGAGGTCGTCGAGATGACCGACGGCGAGGAGGCCGGGATCAAGGGCGCCACGCTCCTGATCAAGGGTCACAACGCCTACG
GGTGGCTCAAGACCGAGTCCGGCGTGCACCGGCTGGTGCGGATCTCGCCCTACGATTCCAGCGCGCGGCGGCACACCAGC
TTCGCCAGCGTCTGGGTCTACCCGGTGATCGACGACCGGATCGAGATCGAGATCAAGGAATCCGACTGCCGGATCGACAC
CTACCGGTCGTCCGGCGCCGGCGGCCAGCACGTCAACACCACCGACTCGGCGGTGCGCATCACCCACAATCCCACCGGGA
TCGTGGTGGCGTGTCAGCAGGAGCGTTCGCAGCACAAGAACCGGGCGACCGCCTGGAACATGCTGCGCGCCCGCCTGTAC
GAGCTGGAGCTGAAGAAGCGCGAGGAGAAGGCCAACGCCGAGGCCGCCGCCAAGACGGATATCGGCTGGGGCCACCAGAT
CCGGTCCTACGTGCTGCAGCCGTACCAGCTGGTGAAGGACCTGCGCACCGGCACCCAGTCCACCGATCCGGACGAGGTGC
TCGACGGCGACCTCGACCCGTTCATGGAGGCCTCGCTGGCCCAGCGGGTCTACGGCGGCGGCGAGGCGGTCGAGGATATC
GACTGA

Upstream 100 bases:

>100_bases
CATCTTTGACTGGGACACAGTCGATAAACGCCTCGCGGAGCTGAACGCAGCCTCCGAGGACCCCGACCTCTGGAACGACG
CCGAGGCCGCCCAGAAGGTC

Downstream 100 bases:

>100_bases
TCGGCGGTCTCGATCCGCAACGCCGTCATCACGAGCGGAGCGAAGTGGCCCAGGGCAGCACGCGATCGCTCAGCGTGGCG
CCACCCGGGATCGCTTCGCT

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MRERQQLDEAVTAIKKLERDLEDGATLIELGEMEGDESSIREGEAAILAVEKEAASRQVETLLSGEADGFDTYLEVHAGA
GGTESQDWANMLQRMYARWAERRKYKVEVVEMTDGEEAGIKGATLLIKGHNAYGWLKTESGVHRLVRISPYDSSARRHTS
FASVWVYPVIDDRIEIEIKESDCRIDTYRSSGAGGQHVNTTDSAVRITHNPTGIVVACQQERSQHKNRATAWNMLRARLY
ELELKKREEKANAEAAAKTDIGWGHQIRSYVLQPYQLVKDLRTGTQSTDPDEVLDGDLDPFMEASLAQRVYGGGEAVEDI
D

Sequences:

>Translated_321_residues
MRERQQLDEAVTAIKKLERDLEDGATLIELGEMEGDESSIREGEAAILAVEKEAASRQVETLLSGEADGFDTYLEVHAGA
GGTESQDWANMLQRMYARWAERRKYKVEVVEMTDGEEAGIKGATLLIKGHNAYGWLKTESGVHRLVRISPYDSSARRHTS
FASVWVYPVIDDRIEIEIKESDCRIDTYRSSGAGGQHVNTTDSAVRITHNPTGIVVACQQERSQHKNRATAWNMLRARLY
ELELKKREEKANAEAAAKTDIGWGHQIRSYVLQPYQLVKDLRTGTQSTDPDEVLDGDLDPFMEASLAQRVYGGGEAVEDI
D
>Mature_321_residues
MRERQQLDEAVTAIKKLERDLEDGATLIELGEMEGDESSIREGEAAILAVEKEAASRQVETLLSGEADGFDTYLEVHAGA
GGTESQDWANMLQRMYARWAERRKYKVEVVEMTDGEEAGIKGATLLIKGHNAYGWLKTESGVHRLVRISPYDSSARRHTS
FASVWVYPVIDDRIEIEIKESDCRIDTYRSSGAGGQHVNTTDSAVRITHNPTGIVVACQQERSQHKNRATAWNMLRARLY
ELELKKREEKANAEAAAKTDIGWGHQIRSYVLQPYQLVKDLRTGTQSTDPDEVLDGDLDPFMEASLAQRVYGGGEAVEDI
D

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=299, Percent_Identity=37.7926421404682, Blast_Score=165, Evalue=5e-41,
Organism=Homo sapiens, GI34577120, Length=237, Percent_Identity=39.662447257384, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI166795305, Length=211, Percent_Identity=37.4407582938389, Blast_Score=119, Evalue=3e-27,
Organism=Escherichia coli, GI2367172, Length=305, Percent_Identity=54.0983606557377, Blast_Score=340, Evalue=7e-95,
Organism=Escherichia coli, GI1787462, Length=240, Percent_Identity=46.6666666666667, Blast_Score=195, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI17542784, Length=207, Percent_Identity=32.3671497584541, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6321295, Length=294, Percent_Identity=40.1360544217687, Blast_Score=153, Evalue=4e-38,
Organism=Drosophila melanogaster, GI19921226, Length=196, Percent_Identity=42.8571428571429, Blast_Score=150, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 35888; Mature: 35888

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRERQQLDEAVTAIKKLERDLEDGATLIELGEMEGDESSIREGEAAILAVEKEAASRQVE
CCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHCCCCEEEEEEEHHHHHHHHH
TLLSGEADGFDTYLEVHAGAGGTESQDWANMLQRMYARWAERRKYKVEVVEMTDGEEAGI
HHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCC
KGATLLIKGHNAYGWLKTESGVHRLVRISPYDSSARRHTSFASVWVYPVIDDRIEIEIKE
CCEEEEEECCCCEEEEECCCCCEEEEEECCCCCCHHHCCCEEEEEEEEEECCEEEEEEEC
SDCRIDTYRSSGAGGQHVNTTDSAVRITHNPTGIVVACQQERSQHKNRATAWNMLRARLY
CCCEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEEECHHHHHHHHHHHHHHHHHHHHH
ELELKKREEKANAEAAAKTDIGWGHQIRSYVLQPYQLVKDLRTGTQSTDPDEVLDGDLDP
HHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHCHHHHHHHHHCCCCCCCHHHHHCCCCCH
FMEASLAQRVYGGGEAVEDID
HHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRERQQLDEAVTAIKKLERDLEDGATLIELGEMEGDESSIREGEAAILAVEKEAASRQVE
CCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHCCCCEEEEEEEHHHHHHHHH
TLLSGEADGFDTYLEVHAGAGGTESQDWANMLQRMYARWAERRKYKVEVVEMTDGEEAGI
HHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCC
KGATLLIKGHNAYGWLKTESGVHRLVRISPYDSSARRHTSFASVWVYPVIDDRIEIEIKE
CCEEEEEECCCCEEEEECCCCCEEEEEECCCCCCHHHCCCEEEEEEEEEECCEEEEEEEC
SDCRIDTYRSSGAGGQHVNTTDSAVRITHNPTGIVVACQQERSQHKNRATAWNMLRARLY
CCCEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEEECHHHHHHHHHHHHHHHHHHHHH
ELELKKREEKANAEAAAKTDIGWGHQIRSYVLQPYQLVKDLRTGTQSTDPDEVLDGDLDP
HHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHCHHHHHHHHHCCCCCCCHHHHHCCCCCH
FMEASLAQRVYGGGEAVEDID
HHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA