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The map label for this gene is dhaA [H]

Identifier: 170751070

GI number: 170751070

Start: 4987354

End: 4988319

Strand: Reverse

Name: dhaA [H]

Synonym: Mrad2831_4681

Alternate gene names: 170751070

Gene position: 4988319-4987354 (Counterclockwise)

Preceding gene: 170751071

Following gene: 170751068

Centisome position: 82.07

GC content: 67.08

Gene sequence:

>966_bases
ATGAAACACCTTCTGCTTGCCCTGATGCTCACGACGGCCGGCACCGTCTTTCCCCCTCATGCCAAGGCGGAGCAGGCCCC
GGTCGCTATCCAGGCACCGGCGACGGTCCACTACCGTTCCGCGACGGTCGACGGCGTGAAGGTCTTCTACCGCGAGGCCG
GCCCGGCCGACGGCCCGGTCGTGCTGCTCCTGCACGGCTTCCCGACCTCCTCGCACATGTTCCGCAACCTGATCCCGACG
CTCGCGGACCGCTACCGCGTCATCGCGCCCGACTACCCCGGCTTCGGCCAGAGCGACGCGCCTGACCACACCCAGTTCGC
CTACACCTTCGGCCACTACGCCGACCTTGTGGACGGCCTGCTCGGCCAACTCGGCGCCACCTCCTACGCGATGTACGTGA
TGGATTACGGCGCGCCGGTCGGCTACCGCCTCGCCCTCAAGCACCCCGACCGGGTGACCGGCCTGATCGTCCAGAACGGC
AACGCCTACGACGAGGGGTTGCGCGAGTTCTGGGATCCGATCAAGGCGTACTGGGCCGACGGCTCGGCCAAGAGCCGCGA
GGCGCTGTCGGGTCTCGTGACCCTCCCCACGACGATCTTCCAGTACACGGACGGCGTGCGGGACGTCTCCAGGATCAGCC
CGGACAACTGGGTCCACGACCAGGCCCTGCTCGACCGGCCCGGCAACAAGGATGTCCAGCTCGACCTGTTCTACGACTAC
CGGACGAACGTGCCGCTCTACCCGCAATTCCAGGCATTCTTCCGCGAGCGCAAGCCGCCGACGCTGATCGTCTGGGGCAA
GAACGACAAGATCTTCCCCGAGGAAGGGGCGCACCCCTACCTGAGGGACCTGCCGGACGCGGAGCTGCACATCCTCGATA
CTGGCCACTTCGCGCTGGAGGACAAGCTGGACGAGATGGCGCCCCTCATCCGCGGCTTCCTCGACAAGCAGGTGGCGAAG
CGCTGA

Upstream 100 bases:

>100_bases
ATGCCTTCCACACGAAGCCGATTAACGCCTGCAAGCTGAAGACCGATGATGTCTCCATGAGGCGGCGAGCTCCTCGACGC
CCCCACGAAAGGAGACACCG

Downstream 100 bases:

>100_bases
CCCTTCTGACGGGTCTGGCTGGCAGGCGCGACGCGGACCGCGCCCCTTACATGGGGGTGCGGTCCTTGGCATTCCCGCCG
CGTTTCGGTGACCGTGAGAG

Product: alpha/beta hydrolase fold protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MKHLLLALMLTTAGTVFPPHAKAEQAPVAIQAPATVHYRSATVDGVKVFYREAGPADGPVVLLLHGFPTSSHMFRNLIPT
LADRYRVIAPDYPGFGQSDAPDHTQFAYTFGHYADLVDGLLGQLGATSYAMYVMDYGAPVGYRLALKHPDRVTGLIVQNG
NAYDEGLREFWDPIKAYWADGSAKSREALSGLVTLPTTIFQYTDGVRDVSRISPDNWVHDQALLDRPGNKDVQLDLFYDY
RTNVPLYPQFQAFFRERKPPTLIVWGKNDKIFPEEGAHPYLRDLPDAELHILDTGHFALEDKLDEMAPLIRGFLDKQVAK
R

Sequences:

>Translated_321_residues
MKHLLLALMLTTAGTVFPPHAKAEQAPVAIQAPATVHYRSATVDGVKVFYREAGPADGPVVLLLHGFPTSSHMFRNLIPT
LADRYRVIAPDYPGFGQSDAPDHTQFAYTFGHYADLVDGLLGQLGATSYAMYVMDYGAPVGYRLALKHPDRVTGLIVQNG
NAYDEGLREFWDPIKAYWADGSAKSREALSGLVTLPTTIFQYTDGVRDVSRISPDNWVHDQALLDRPGNKDVQLDLFYDY
RTNVPLYPQFQAFFRERKPPTLIVWGKNDKIFPEEGAHPYLRDLPDAELHILDTGHFALEDKLDEMAPLIRGFLDKQVAK
R
>Mature_321_residues
MKHLLLALMLTTAGTVFPPHAKAEQAPVAIQAPATVHYRSATVDGVKVFYREAGPADGPVVLLLHGFPTSSHMFRNLIPT
LADRYRVIAPDYPGFGQSDAPDHTQFAYTFGHYADLVDGLLGQLGATSYAMYVMDYGAPVGYRLALKHPDRVTGLIVQNG
NAYDEGLREFWDPIKAYWADGSAKSREALSGLVTLPTTIFQYTDGVRDVSRISPDNWVHDQALLDRPGNKDVQLDLFYDY
RTNVPLYPQFQAFFRERKPPTLIVWGKNDKIFPEEGAHPYLRDLPDAELHILDTGHFALEDKLDEMAPLIRGFLDKQVAK
R

Specific function: Catalyzes hydrolytic cleavage of carbon-halogen bonds in halogenated aliphatic compounds, leading to the formation of the corresponding primary alcohols, halide ions and protons [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the haloalkane dehalogenase family. Type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI218777837, Length=317, Percent_Identity=24.2902208201893, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI218777840, Length=285, Percent_Identity=25.6140350877193, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI13376164, Length=285, Percent_Identity=25.6140350877193, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324392, Length=271, Percent_Identity=47.6014760147601, Blast_Score=259, Evalue=4e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.8.1.5 [H]

Molecular weight: Translated: 35930; Mature: 35930

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHLLLALMLTTAGTVFPPHAKAEQAPVAIQAPATVHYRSATVDGVKVFYREAGPADGPV
CHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCEEEEECCCCCHHHHEEECCCCCCCCE
VLLLHGFPTSSHMFRNLIPTLADRYRVIAPDYPGFGQSDAPDHTQFAYTFGHYADLVDGL
EEEEECCCCHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHH
LGQLGATSYAMYVMDYGAPVGYRLALKHPDRVTGLIVQNGNAYDEGLREFWDPIKAYWAD
HHHHCCCCEEEEEEECCCCCCEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCC
GSAKSREALSGLVTLPTTIFQYTDGVRDVSRISPDNWVHDQALLDRPGNKDVQLDLFYDY
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHCCCCCCCEEEEEEEEE
RTNVPLYPQFQAFFRERKPPTLIVWGKNDKIFPEEGAHPYLRDLPDAELHILDTGHFALE
CCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHCCCCCEEEEEECCCCHHH
DKLDEMAPLIRGFLDKQVAKR
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKHLLLALMLTTAGTVFPPHAKAEQAPVAIQAPATVHYRSATVDGVKVFYREAGPADGPV
CHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCEEEEECCCCCHHHHEEECCCCCCCCE
VLLLHGFPTSSHMFRNLIPTLADRYRVIAPDYPGFGQSDAPDHTQFAYTFGHYADLVDGL
EEEEECCCCHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHH
LGQLGATSYAMYVMDYGAPVGYRLALKHPDRVTGLIVQNGNAYDEGLREFWDPIKAYWAD
HHHHCCCCEEEEEEECCCCCCEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCC
GSAKSREALSGLVTLPTTIFQYTDGVRDVSRISPDNWVHDQALLDRPGNKDVQLDLFYDY
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHCCCCCCCEEEEEEEEE
RTNVPLYPQFQAFFRERKPPTLIVWGKNDKIFPEEGAHPYLRDLPDAELHILDTGHFALE
CCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHCCCCCEEEEEECCCCHHH
DKLDEMAPLIRGFLDKQVAKR
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]