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The map label for this gene is ycjG [H]

Identifier: 170751047

GI number: 170751047

Start: 4959032

End: 4960015

Strand: Direct

Name: ycjG [H]

Synonym: Mrad2831_4658

Alternate gene names: 170751047

Gene position: 4959032-4960015 (Clockwise)

Preceding gene: 170751046

Following gene: 170751048

Centisome position: 81.59

GC content: 74.8

Gene sequence:

>984_bases
ATGGCGCGCCGCCTGACCCTCGCCGTCGAGCGCTTTCCCATCGCCGGCGCCTTCACGATCTCGCGCGGCAGCCGCACCGA
GATCGCCGTCGTGGTGGCGACGATCGCGGACGGCGACGCGGTCGGGCGCGGCGAGTGCGTGCCCTATCCGCGCTACGGCG
AGAGCGTCGAGAGCGTCAGCGCCCTGATCGAGGGCCAGGCGGAGGCGATCGCCGCCGGGATCGAGCGGGACGCGCTCATG
GCCCGCATGAAGGCGGGCGCCGCGCGCAACGCCCTCGACTGCGCGCTGTTCGACCTCGAGGCGAAGCGCCAGGGCCGCCC
CGCCTGGGAGATCGCGGGTCTCGCCGAGCCCCGGCCGGCGATCACCGCCTACACGCTGAGCCTCGGCACGCCGGAGAGCA
TGGCGGAGGCCGCCCGCAAGTCCGCCCACCGGCCGCTGCTCAAGGTCAAGCTCGGCGGGGACGGCGATCCGGAGCGCATC
GCCGCGGTGCGGGCCGGCGCGCCGGAATCCCGGCTGATCGTCGACGCCAACGAGGCGTGGCGGCCCGAGAACGTCGAGGC
CAACCTCGCGGCCTGCCTGAAGGCGGGCGTCGCGCTGATCGAGCAGCCGCTGCCGGCCGACGCCGACGCCCTGCTGGCCG
AGATCCCCCACCCGGTGCCGGTCTGCGCCGACGAGAGCCTGCACGACCGTGCCGGACTCGACGCGCTGGCCGGGCGCTAC
GACGCGATCAACATCAAGCTCGACAAGACCGGCGGCCTGACCGAGGCGGTGCTCCTCGCCCGCGAGGCGCGGGCCCGGGG
GCTCTCGATCATGGTCGGCTGCATGCTCGGCACCTCGCTGGGCATGGCGCCGGCGGCACTGCTCGCCAACGCCGCGGAGT
TCGTCGACCTCGACGGACCGCTGCTGCTGGCCTGCGACCGTGACCCGCCGCTGCGCTACGAGGGCAGCCTGATGCACCCG
CCGACGCCGGAGCTCTGGGGCTAG

Upstream 100 bases:

>100_bases
AAGCTCGCGTCCGAGCACGGCCTGCCCGCCACCGACCCGGTGCGCTTCGGCGTCGACGGGCTGGTCGACCGGATCGTCGC
CGACTTCCCGGAGGCCTGAC

Downstream 100 bases:

>100_bases
GGCACGCGGGCGGAGCGGTCGCCGTCCGCGGTCCGAGACCGCCCAGCGCGAGATCCCGGAGGCTCGGCCCTGTGGAGATC
CCAGGTGCGGACGATAGGGA

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MARRLTLAVERFPIAGAFTISRGSRTEIAVVVATIADGDAVGRGECVPYPRYGESVESVSALIEGQAEAIAAGIERDALM
ARMKAGAARNALDCALFDLEAKRQGRPAWEIAGLAEPRPAITAYTLSLGTPESMAEAARKSAHRPLLKVKLGGDGDPERI
AAVRAGAPESRLIVDANEAWRPENVEANLAACLKAGVALIEQPLPADADALLAEIPHPVPVCADESLHDRAGLDALAGRY
DAINIKLDKTGGLTEAVLLAREARARGLSIMVGCMLGTSLGMAPAALLANAAEFVDLDGPLLLACDRDPPLRYEGSLMHP
PTPELWG

Sequences:

>Translated_327_residues
MARRLTLAVERFPIAGAFTISRGSRTEIAVVVATIADGDAVGRGECVPYPRYGESVESVSALIEGQAEAIAAGIERDALM
ARMKAGAARNALDCALFDLEAKRQGRPAWEIAGLAEPRPAITAYTLSLGTPESMAEAARKSAHRPLLKVKLGGDGDPERI
AAVRAGAPESRLIVDANEAWRPENVEANLAACLKAGVALIEQPLPADADALLAEIPHPVPVCADESLHDRAGLDALAGRY
DAINIKLDKTGGLTEAVLLAREARARGLSIMVGCMLGTSLGMAPAALLANAAEFVDLDGPLLLACDRDPPLRYEGSLMHP
PTPELWG
>Mature_326_residues
ARRLTLAVERFPIAGAFTISRGSRTEIAVVVATIADGDAVGRGECVPYPRYGESVESVSALIEGQAEAIAAGIERDALMA
RMKAGAARNALDCALFDLEAKRQGRPAWEIAGLAEPRPAITAYTLSLGTPESMAEAARKSAHRPLLKVKLGGDGDPERIA
AVRAGAPESRLIVDANEAWRPENVEANLAACLKAGVALIEQPLPADADALLAEIPHPVPVCADESLHDRAGLDALAGRYD
AINIKLDKTGGLTEAVLLAREARARGLSIMVGCMLGTSLGMAPAALLANAAEFVDLDGPLLLACDRDPPLRYEGSLMHPP
TPELWG

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has a role in the recycling of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of all the L-Ala-X dipeptides, excep

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=319, Percent_Identity=48.2758620689655, Blast_Score=277, Evalue=6e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 34317; Mature: 34186

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRLTLAVERFPIAGAFTISRGSRTEIAVVVATIADGDAVGRGECVPYPRYGESVESVS
CCCEEEEEEECCCCCEEEEECCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHH
ALIEGQAEAIAAGIERDALMARMKAGAARNALDCALFDLEAKRQGRPAWEIAGLAEPRPA
HHHHCCHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEEECHHHHCCCCCEEECCCCCCCCC
ITAYTLSLGTPESMAEAARKSAHRPLLKVKLGGDGDPERIAAVRAGAPESRLIVDANEAW
EEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHEEEEECCCCCCEEEEECCCCC
RPENVEANLAACLKAGVALIEQPLPADADALLAEIPHPVPVCADESLHDRAGLDALAGRY
CCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCHHHCCHHHHHCCC
DAINIKLDKTGGLTEAVLLAREARARGLSIMVGCMLGTSLGMAPAALLANAAEFVDLDGP
EEEEEEEECCCCHHHHHHHHHHHHHCCHHEEEHHHHHCCCCCHHHHHHHCHHHHEECCCC
LLLACDRDPPLRYEGSLMHPPTPELWG
EEEEECCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ARRLTLAVERFPIAGAFTISRGSRTEIAVVVATIADGDAVGRGECVPYPRYGESVESVS
CCEEEEEEECCCCCEEEEECCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHH
ALIEGQAEAIAAGIERDALMARMKAGAARNALDCALFDLEAKRQGRPAWEIAGLAEPRPA
HHHHCCHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEEECHHHHCCCCCEEECCCCCCCCC
ITAYTLSLGTPESMAEAARKSAHRPLLKVKLGGDGDPERIAAVRAGAPESRLIVDANEAW
EEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHEEEEECCCCCCEEEEECCCCC
RPENVEANLAACLKAGVALIEQPLPADADALLAEIPHPVPVCADESLHDRAGLDALAGRY
CCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCHHHCCHHHHHCCC
DAINIKLDKTGGLTEAVLLAREARARGLSIMVGCMLGTSLGMAPAALLANAAEFVDLDGP
EEEEEEEECCCCHHHHHHHHHHHHHCCHHEEEHHHHHCCCCCHHHHHHHCHHHHEECCCC
LLLACDRDPPLRYEGSLMHPPTPELWG
EEEEECCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7499381; 9097039; 9278503 [H]