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The map label for this gene is exoK [H]

Identifier: 170750989

GI number: 170750989

Start: 4895155

End: 4895988

Strand: Reverse

Name: exoK [H]

Synonym: Mrad2831_4600

Alternate gene names: 170750989

Gene position: 4895988-4895155 (Counterclockwise)

Preceding gene: 170750991

Following gene: 170750988

Centisome position: 80.55

GC content: 70.26

Gene sequence:

>834_bases
ATGACGATCTGGTCCGCCCCGGGCCGCCGCCTCTGCGTGGCGGCGACGCTTCTCCTGCAGGCCTACCCCGCGAGCGCGGG
CGAGACAGCCGCGCCGCCGGCCGCCGCTCCGGCCGCCCAGGGCGGCCCGTCCTTCGTGGAGCTCTTCAGCAGCCTGAACG
ACCGCCGCTGGTACGTCTCGGACGGCTGGGTCAACGGCGACTGGCAGGGCTGCACGTGGTCGAAGCACCGCGCCCGGATC
GCGACGCCCGGCGCTCTCTCGCTGTCCCTGACGGACATCACCTACAAGGAGCGCCCGTTCAGCTGCGCCGAGATCCAGAC
CCGCGAGCGCTACGGCTACGGCACCTACGAAGTCCGGATGCGGGCCGGGGCCGGGTCCGGCATGGTCTCGGCGTTCTTCA
CCTATAACGGCCCCGAGAACGGCGACCGGCGGACGAACGACGAGATCGATTTCGAGTGGCTCGGCAAGGACACGAGCAAG
GTCCAGCTCAACTACTTCGTCGGCGGCGTCGGCGAGCATGTCAGCCTCGACGCCCTCGGCTTCGACGCGGCGACCACCAC
CGCGGACTACGCCTTCGAGTGGCTGCCCGACCGCCTGCGCTGGTACGTCAACGGGCGCCTGCTGCGCGAGGTCATCGGCA
CGCCCGACCGGCCGATCCCGTCGCACCCGAGCAAGATCATGCTCAGCGTCTGGTCGGGACAGGGCCCGGACTTCGCCTCC
TGGCTCGGACCGACGGAGTACTCGGGACAGCCGGTCACCGCGCTGTTCGAGCGGGTCGCGTTCACCCGGATGGGCGATCC
GTGCGGCTTCAAGGAATCGATCGTCTGCCGCTAG

Upstream 100 bases:

>100_bases
GCCCGGGCCGGATCGTCCGAATGATCGCCGGCCGGCATTGCCCCCGCCGCTACGATGCGCGCCGCCGCGATCCTGCGGCG
ACTGGTTTGAGGACGTGCGC

Downstream 100 bases:

>100_bases
CCACACCGGCGGGCGGACGCCGCACCGGAGACCGCCCCTGACATCGACCCGGACATCGCGCCGGACATCGCCCCGCACAC
GGCTCCGCACAGGCCCCCGC

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: Succinoglycan biosynthesis protein exoK [H]

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MTIWSAPGRRLCVAATLLLQAYPASAGETAAPPAAAPAAQGGPSFVELFSSLNDRRWYVSDGWVNGDWQGCTWSKHRARI
ATPGALSLSLTDITYKERPFSCAEIQTRERYGYGTYEVRMRAGAGSGMVSAFFTYNGPENGDRRTNDEIDFEWLGKDTSK
VQLNYFVGGVGEHVSLDALGFDAATTTADYAFEWLPDRLRWYVNGRLLREVIGTPDRPIPSHPSKIMLSVWSGQGPDFAS
WLGPTEYSGQPVTALFERVAFTRMGDPCGFKESIVCR

Sequences:

>Translated_277_residues
MTIWSAPGRRLCVAATLLLQAYPASAGETAAPPAAAPAAQGGPSFVELFSSLNDRRWYVSDGWVNGDWQGCTWSKHRARI
ATPGALSLSLTDITYKERPFSCAEIQTRERYGYGTYEVRMRAGAGSGMVSAFFTYNGPENGDRRTNDEIDFEWLGKDTSK
VQLNYFVGGVGEHVSLDALGFDAATTTADYAFEWLPDRLRWYVNGRLLREVIGTPDRPIPSHPSKIMLSVWSGQGPDFAS
WLGPTEYSGQPVTALFERVAFTRMGDPCGFKESIVCR
>Mature_276_residues
TIWSAPGRRLCVAATLLLQAYPASAGETAAPPAAAPAAQGGPSFVELFSSLNDRRWYVSDGWVNGDWQGCTWSKHRARIA
TPGALSLSLTDITYKERPFSCAEIQTRERYGYGTYEVRMRAGAGSGMVSAFFTYNGPENGDRRTNDEIDFEWLGKDTSKV
QLNYFVGGVGEHVSLDALGFDAATTTADYAFEWLPDRLRWYVNGRLLREVIGTPDRPIPSHPSKIMLSVWSGQGPDFASW
LGPTEYSGQPVTALFERVAFTRMGDPCGFKESIVCR

Specific function: Cleaves high molecular weight succinoglycan to yield LMW succinoglycan. Dynamically regulate the molecular weight distribution of succinoglycan by cleaving nascent succinoglycan only during a limited period after its synthesis, perhaps before it undergoes

COG id: COG2273

COG function: function code G; Beta-glucanase/Beta-glucan synthetase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 16 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6321628, Length=179, Percent_Identity=32.4022346368715, Blast_Score=85, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323242, Length=152, Percent_Identity=30.9210526315789, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008264
- InterPro:   IPR008985
- InterPro:   IPR013320
- InterPro:   IPR000757
- InterPro:   IPR008263 [H]

Pfam domain/function: PF00722 Glyco_hydro_16 [H]

EC number: NA

Molecular weight: Translated: 30532; Mature: 30401

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS01034 GLYCOSYL_HYDROL_F16

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIWSAPGRRLCVAATLLLQAYPASAGETAAPPAAAPAAQGGPSFVELFSSLNDRRWYVS
CCEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEE
DGWVNGDWQGCTWSKHRARIATPGALSLSLTDITYKERPFSCAEIQTRERYGYGTYEVRM
CCEECCCCCCCCCCCCCCEEECCCEEEEEEEECEECCCCCCHHHHHHHHHCCCCEEEEEE
RAGAGSGMVSAFFTYNGPENGDRRTNDEIDFEWLGKDTSKVQLNYFVGGVGEHVSLDALG
ECCCCCCEEEEEEEECCCCCCCCCCCCCCCEEECCCCCCEEEEEEEECCCCCEEEEEEEC
FDAATTTADYAFEWLPDRLRWYVNGRLLREVIGTPDRPIPSHPSKIMLSVWSGQGPDFAS
CCCHHCHHHHHHHHCHHHHEEHHCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCHHH
WLGPTEYSGQPVTALFERVAFTRMGDPCGFKESIVCR
HCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TIWSAPGRRLCVAATLLLQAYPASAGETAAPPAAAPAAQGGPSFVELFSSLNDRRWYVS
CEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEE
DGWVNGDWQGCTWSKHRARIATPGALSLSLTDITYKERPFSCAEIQTRERYGYGTYEVRM
CCEECCCCCCCCCCCCCCEEECCCEEEEEEEECEECCCCCCHHHHHHHHHCCCCEEEEEE
RAGAGSGMVSAFFTYNGPENGDRRTNDEIDFEWLGKDTSKVQLNYFVGGVGEHVSLDALG
ECCCCCCEEEEEEEECCCCCCCCCCCCCCCEEECCCCCCEEEEEEEECCCCCEEEEEEEC
FDAATTTADYAFEWLPDRLRWYVNGRLLREVIGTPDRPIPSHPSKIMLSVWSGQGPDFAS
CCCHHCHHHHHHHHCHHHHEEHHCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCHHH
WLGPTEYSGQPVTALFERVAFTRMGDPCGFKESIVCR
HCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8226645; 8479421; 11481431; 9560202 [H]