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The map label for this gene is pleD [H]

Identifier: 170750974

GI number: 170750974

Start: 4880969

End: 4881814

Strand: Direct

Name: pleD [H]

Synonym: Mrad2831_4585

Alternate gene names: 170750974

Gene position: 4880969-4881814 (Clockwise)

Preceding gene: 170750973

Following gene: 170750975

Centisome position: 80.31

GC content: 74.47

Gene sequence:

>846_bases
GTGAGCCCGCACCGGGCGCCGGCCCACGATCTCCTGTCCGAGCCGGCGGACGCCTTCAGCCTGTTCGACACCGAGGAGGC
CGTCCTCGGCCGGTCCGAGGTGATGCTGGCCCGCCTCGCCGAGGTCGGCGACGGGGTGCGGGAGCTCGCCGGCGCCTACC
GGCGCAGCTACCACGAGCAGCGCCGGCTGGTGCGGCTGAGCGACCGGATGCAGCTCGACCTGCACCGGGCCAACCAGCGG
CTCGCCGAGCAGCAGCGCGCCCTGCGCCACCTCAACGACGCCCTCTCGGCGGAGATCGAGCACCGGGCGCGCCTCGAGGC
CGAGCTCCGCCGCCTCGCCGACACCGACCACCTCACCGACGCCCTGTCGCGCCGCCGCTTCCTGCAGATCTGCGAGGAGG
TCTGGGCCCGGACGCAGTCGGGCGACACGCCCTCCAGCGTGCTGATGCTCGACCTCGACCGCTTCAAGCAGATCAACGAC
CGCTACGGCCACAGCGCGGGTGACACGGTCCTCAAGGCCTTCGTCGAGACCTGCCGCGACAGCCTGCGCGCGGTCGACGT
GATCGGCCGCATGGGCGGCGAGGAATTCGCCATCCTCGTCACCGGCACCGTGCTGGCCGAGGCGCAGGCCATCGCGGAGC
AGCTCCGCCACGCGGTGGCGCAGCGGCCCGTGCGCCTGGAGCACGGGCCGCTGCCGATCTCGGTGAGCATCGGCCTCGCG
GCGTTCGGCCCGAGCGAGTCGATGTCGGCGACGCTCCGGCGGGCCGACGCGGCGCTCTACGCCGCCAAGCACGCCGGCCG
CGACTGCGTGCGCTGCGCGGCGGAGCCGGGCGCGTCCCTGACCTGA

Upstream 100 bases:

>100_bases
GGATGTTCGGCGCCGACGACGACGTGATCCAGGAGGCCGGCGAGGATTTCGCGGCCGATTTCGCGCATGCCCGGTTCGAG
ATGATCGCGAGCGTCCCCGC

Downstream 100 bases:

>100_bases
GGTGCCGCCCGGCGCATGCGCGCAACCGCTGTGACACGGAGTGACGTCGCGTGAGCGGACCTGACACGGCCCTGGACAGC
CCGCGTCCCGTCGGGGGGAT

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MSPHRAPAHDLLSEPADAFSLFDTEEAVLGRSEVMLARLAEVGDGVRELAGAYRRSYHEQRRLVRLSDRMQLDLHRANQR
LAEQQRALRHLNDALSAEIEHRARLEAELRRLADTDHLTDALSRRRFLQICEEVWARTQSGDTPSSVLMLDLDRFKQIND
RYGHSAGDTVLKAFVETCRDSLRAVDVIGRMGGEEFAILVTGTVLAEAQAIAEQLRHAVAQRPVRLEHGPLPISVSIGLA
AFGPSESMSATLRRADAALYAAKHAGRDCVRCAAEPGASLT

Sequences:

>Translated_281_residues
MSPHRAPAHDLLSEPADAFSLFDTEEAVLGRSEVMLARLAEVGDGVRELAGAYRRSYHEQRRLVRLSDRMQLDLHRANQR
LAEQQRALRHLNDALSAEIEHRARLEAELRRLADTDHLTDALSRRRFLQICEEVWARTQSGDTPSSVLMLDLDRFKQIND
RYGHSAGDTVLKAFVETCRDSLRAVDVIGRMGGEEFAILVTGTVLAEAQAIAEQLRHAVAQRPVRLEHGPLPISVSIGLA
AFGPSESMSATLRRADAALYAAKHAGRDCVRCAAEPGASLT
>Mature_280_residues
SPHRAPAHDLLSEPADAFSLFDTEEAVLGRSEVMLARLAEVGDGVRELAGAYRRSYHEQRRLVRLSDRMQLDLHRANQRL
AEQQRALRHLNDALSAEIEHRARLEAELRRLADTDHLTDALSRRRFLQICEEVWARTQSGDTPSSVLMLDLDRFKQINDR
YGHSAGDTVLKAFVETCRDSLRAVDVIGRMGGEEFAILVTGTVLAEAQAIAEQLRHAVAQRPVRLEHGPLPISVSIGLAA
FGPSESMSATLRRADAALYAAKHAGRDCVRCAAEPGASLT

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=177, Percent_Identity=40.1129943502825, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI145693134, Length=236, Percent_Identity=30.9322033898305, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786584, Length=177, Percent_Identity=33.8983050847458, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787262, Length=172, Percent_Identity=35.4651162790698, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI87082007, Length=175, Percent_Identity=36, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787802, Length=161, Percent_Identity=31.6770186335404, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788085, Length=168, Percent_Identity=33.3333333333333, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1787816, Length=126, Percent_Identity=34.1269841269841, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1787541, Length=178, Percent_Identity=31.4606741573034, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787056, Length=157, Percent_Identity=32.484076433121, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788381, Length=195, Percent_Identity=29.2307692307692, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI87081977, Length=155, Percent_Identity=28.3870967741935, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 31089; Mature: 30957

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPHRAPAHDLLSEPADAFSLFDTEEAVLGRSEVMLARLAEVGDGVRELAGAYRRSYHEQ
CCCCCCCHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
RRLVRLSDRMQLDLHRANQRLAEQQRALRHLNDALSAEIEHRARLEAELRRLADTDHLTD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
ALSRRRFLQICEEVWARTQSGDTPSSVLMLDLDRFKQINDRYGHSAGDTVLKAFVETCRD
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
SLRAVDVIGRMGGEEFAILVTGTVLAEAQAIAEQLRHAVAQRPVRLEHGPLPISVSIGLA
HHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCEEEECCEE
AFGPSESMSATLRRADAALYAAKHAGRDCVRCAAEPGASLT
ECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SPHRAPAHDLLSEPADAFSLFDTEEAVLGRSEVMLARLAEVGDGVRELAGAYRRSYHEQ
CCCCCCHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
RRLVRLSDRMQLDLHRANQRLAEQQRALRHLNDALSAEIEHRARLEAELRRLADTDHLTD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
ALSRRRFLQICEEVWARTQSGDTPSSVLMLDLDRFKQINDRYGHSAGDTVLKAFVETCRD
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
SLRAVDVIGRMGGEEFAILVTGTVLAEAQAIAEQLRHAVAQRPVRLEHGPLPISVSIGLA
HHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCEEEECCEE
AFGPSESMSATLRRADAALYAAKHAGRDCVRCAAEPGASLT
ECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA