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The map label for this gene is gph [C]

Identifier: 170750888

GI number: 170750888

Start: 4781198

End: 4781875

Strand: Direct

Name: gph [C]

Synonym: Mrad2831_4499

Alternate gene names: 170750888

Gene position: 4781198-4781875 (Clockwise)

Preceding gene: 170750887

Following gene: 170750889

Centisome position: 78.67

GC content: 71.53

Gene sequence:

>678_bases
ATGACCGACGCAGCAAAACCCCTCGCGGGCGTGAAGGCCGCGGTGTTCGACGCCTACGGCACGCTGTTCGACGTGAATGC
CGCGGTCCAGCGCTACGCCGAGGCGGTGGGGCCGGACGCCGCCCACCTCTCCGAGGTCTGGCGCAACAAGCAGCTCGAGT
ACAGCTGGACCCTGTCGCTGATGGGCCAGTACGCCGCCTTCTGGGACCTCACCGTGCGGGCCCTCGACTATGCCCTGGCG
ATCCACCCGAACGTCGATCCCGGCCTGCGCGAGCGGCTCCTCGACGCCTACCGCGACCTCGAGGCCTACCCGGAGGTCCC
GGGCGTGCTGGCGGCCCTGCGCAAGCGCGGCATCCGCACCGCCGTCCTCACCAACGGCAACGCCGCCATGGTCGACCGGG
CGGTCGCCTCGGCCGGGCTCGCCGACCACCTCGACGCGGTCCTCTCGGTGGACGACGCGCAGGTGTTCAAGACCCATCCC
GACGCCTACCGGATCGCCCTGACCCGGCTGGCGGTGGGGCAGGGCGACGTGCTGTTCTGCTCGTCGAACCGCTGGGACGT
GGCGGGGGCAAGGGCCTTCGGCTTCCGGACCGCCTGGGTCAACCGCAAGGGGCTGCCCGACGAGTATGCCGACCTCGCGC
CCACGGTCGTGGTCGAGTCCCTCGACGGGCTGCTCTGA

Upstream 100 bases:

>100_bases
TGTTCGGCCTGCGCCTGCGCCGGGCCTGAATGACCCGGCCGGACTCGCCGCCGCGCGGGTCCGGCCCCAGATCGGGCGAG
AGCACCCAGTGAGGAACGCC

Downstream 100 bases:

>100_bases
CGGGCTGTCCGCTGAGCGTCCCCGATCCGAGACGCGGCCGGCGTCGGGACGTCGCCACGGCAGCGTCCCGCCTCAGGCTG
GAGCGACCGCGGGCCGCCTT

Product: haloacid dehalogenase

Products: NA

Alternate protein names: 2-haloalkanoic acid dehalogenase I; DEHCI; Halocarboxylic acid halidohydrolase I; L-2-haloacid dehalogenase I [H]

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MTDAAKPLAGVKAAVFDAYGTLFDVNAAVQRYAEAVGPDAAHLSEVWRNKQLEYSWTLSLMGQYAAFWDLTVRALDYALA
IHPNVDPGLRERLLDAYRDLEAYPEVPGVLAALRKRGIRTAVLTNGNAAMVDRAVASAGLADHLDAVLSVDDAQVFKTHP
DAYRIALTRLAVGQGDVLFCSSNRWDVAGARAFGFRTAWVNRKGLPDEYADLAPTVVVESLDGLL

Sequences:

>Translated_225_residues
MTDAAKPLAGVKAAVFDAYGTLFDVNAAVQRYAEAVGPDAAHLSEVWRNKQLEYSWTLSLMGQYAAFWDLTVRALDYALA
IHPNVDPGLRERLLDAYRDLEAYPEVPGVLAALRKRGIRTAVLTNGNAAMVDRAVASAGLADHLDAVLSVDDAQVFKTHP
DAYRIALTRLAVGQGDVLFCSSNRWDVAGARAFGFRTAWVNRKGLPDEYADLAPTVVVESLDGLL
>Mature_224_residues
TDAAKPLAGVKAAVFDAYGTLFDVNAAVQRYAEAVGPDAAHLSEVWRNKQLEYSWTLSLMGQYAAFWDLTVRALDYALAI
HPNVDPGLRERLLDAYRDLEAYPEVPGVLAALRKRGIRTAVLTNGNAAMVDRAVASAGLADHLDAVLSVDDAQVFKTHPD
AYRIALTRLAVGQGDVLFCSSNRWDVAGARAFGFRTAWVNRKGLPDEYADLAPTVVVESLDGLL

Specific function: Catalyzes the hydrolytic dehalogenation of small (S)-2- haloalkanoic acids to yield the corresponding (R)-2- hydroxyalkanoic acids. Acts on acids of short chain lengths, C(2) to C(4), with inversion of configuration at C-2 [H]

COG id: COG1011

COG function: function code R; Predicted hydrolase (HAD superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. S-2- haloalkanoic acid dehalogenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006388
- InterPro:   IPR006328
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.8.1.2 [H]

Molecular weight: Translated: 24420; Mature: 24289

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDAAKPLAGVKAAVFDAYGTLFDVNAAVQRYAEAVGPDAAHLSEVWRNKQLEYSWTLSL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEHHHH
MGQYAAFWDLTVRALDYALAIHPNVDPGLRERLLDAYRDLEAYPEVPGVLAALRKRGIRT
HHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEE
AVLTNGNAAMVDRAVASAGLADHLDAVLSVDDAQVFKTHPDAYRIALTRLAVGQGDVLFC
EEEECCCHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHCCCCCCEEEE
SSNRWDVAGARAFGFRTAWVNRKGLPDEYADLAPTVVVESLDGLL
CCCCCCCCCCHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TDAAKPLAGVKAAVFDAYGTLFDVNAAVQRYAEAVGPDAAHLSEVWRNKQLEYSWTLSL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEHHHH
MGQYAAFWDLTVRALDYALAIHPNVDPGLRERLLDAYRDLEAYPEVPGVLAALRKRGIRT
HHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEE
AVLTNGNAAMVDRAVASAGLADHLDAVLSVDDAQVFKTHPDAYRIALTRLAVGQGDVLFC
EEEECCCHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHCCCCCCEEEE
SSNRWDVAGARAFGFRTAWVNRKGLPDEYADLAPTVVVESLDGLL
CCCCCCCCCCHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1995594 [H]